1. Correction: Inhibition of cell movement and proliferation by cell–cell contact-induced interaction of Necl-5 with nectin-3
- Author
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Fujito, Tsutomu, Ikeda, Wataru, Kakunaga, Shigeki, Minami, Yukiko, Kajita, Mihoko, Sakamoto, Yasuhisa, Monden, Morito, and Takai, Yoshimi
- Subjects
Nectins ,Correction ,Down-Regulation ,Cell Biology ,Clathrin ,Endocytosis ,Neoplasm Proteins ,Mice ,Antigens, Neoplasm ,Cell Movement ,Cell Adhesion ,NIH 3T3 Cells ,Animals ,RNA, Small Interfering ,Cell Adhesion Molecules ,Cell Proliferation - Abstract
Immunoglobulin-like Necl-5/Tage4/poliovirus receptor (PVR)/CD155, originally identified as the PVR, has been shown to be up-regulated in cancer cells and to enhance growth factor-induced cell movement and proliferation. In addition, Necl-5 heterophilically trans-interacts with nectin-3, a cell-cell adhesion molecule known to form adherens junctions in cooperation with cadherin. We show here that Necl-5 was down-regulated from cell surface upon cell-cell contacts in NIH3T3 cells. This down-regulation of Necl-5 was initiated by its interaction with nectin-3 and was mainly mediated by clathrin-dependent endocytosis. Then, the down-regulation of Necl-5 induced in this way reduced movement and proliferation of NIH3T3 cells. These results indicate that the down-regulation of Necl-5 induced by its interaction with nectin-3 upon cell-cell contacts may be at least one mechanism underlying contact inhibition of cell movement and proliferation.
- Published
- 2020