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Pathogenic Escherichia coli infections have been consistently reported annually. The basic characteristics and genome of the newly isolated ΦCJ20 from swine feces was analyzed. To determine basic characteristics, dotting assays and double-layer agar assays were conducted. Bacteriophage particles were analyzed via transmission electron microscopy. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis was performed to determine the sizes of major structural proteins. The complete genome of the phage was analyzed. Bacteriophage particles were identified as Myoviridae, with a head measuring 110.57 ± 1.89 nm and a contractile tail measuring 107.97 ± 3.20 nm and were found to infect E. coli. Major structural proteins of ΦCJ20 showed two well-pronounced bands of approximately 53.6 and 70.9 kDa. The genome size of ΦCJ20 was 169,884 bp, and 118 of 307 open reading frames were annotated. This study provides a baseline for the development of E. coli infection treatment strategies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10068-021-00906-y.

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We investigated the effects of different levels of dietary Bacillus subtilis (BS) on innate immunity, growth performance, feed utilization, and disease resistance of juvenile olive flounder. A control diet was formulated without probiotic, and three other diets were prepared adding different doses (1 × 10⁷, 3 × 10⁷, and 5 × 10⁷ CFU/g feed) of BS (designated as Control, BS1, BS3, and BS5, respectively). Triplicate groups of fish (initial body weight, 25 g) were fed for 12 weeks. Fish fed BS‐containing diets showed significantly higher nonspecific immune responses than fish fed the control diet. Fish fed the BS5 diet showed significantly higher growth performance and feed utilization efficiency than fish fed the control diet. Fish fed the BS diets showed remarkably higher resistance against Streptococcus iniae than fish fed the control diet. These results indicate that BS supplementation at a dose of 5 × 10⁷ CFU/g diet can improve innate immunity, growth performance, feed utilization, and disease resistance against S. iniae in olive flounder.

Additional file 3. Electrophoresis and Sanger sequencing of the RT-PCR product of an aberrant ATM mRNA.

Additional file 1. Annotation of the number of the exons.

Additional file 2. The Log ratio and the genomic position of the SNP around the breakpoint.

Supplemental material, IMR881239 Supplemental Material for Anesthesia in a patient with Charcot-Marie-Tooth disease with pneumothorax: a case report by Jae Won Kim, Goo Kim, Tae Woo Kim, Woong Han, Jin Hyun Maeng, Chang Young Jeong, Jin Ho Choi and Dong Ho Park in Journal of International Medical Research

Magnetic resonance imaging (MRI) is widely used for the diagnosis, staging, and follow-up of diseases. MRI is a very useful diagnostic tool for musculoskeletal and cerebrovascular imaging, because it has excellent soft tissue contrast and is considered to be safer than other modalities, as it does not expose the body to radiation [1]. However, MRI is not without risks. The presence of a metal implant in the patient’s body during an MRI scan can be dangerous due to excessive magnetic field interactions. Developments in medicine have led to the use of various implants, such as dental and orthopedic implants, in our bodies [2]. In the field of craniofacial surgery, the use of titanium plates and screws in open reduction and internal fixation procedures has exploded since the late 20th century, and titanium is now considered to be the material of choice for implants. Titanium plates are frequently used for cranioplasty, reconstruction of the facial bones, and reconstruction of the orbital bone, because they yield excellent results without posing any major problems in terms of biosafety [3]. Some physicians, however, have questioned whether titanium implants are actually safe for MRI examinations. The greater the number of elderly patients, the higher the probability of conducting brain MRI due to cerebrovascular disease. This has led to the question of whether widespread use of titanium implants in the craniofacial region is acceptable given the likelihood that patients will undergo brain MRI in the future. In this paper, we present the mechanism of MRI and its relationship to metals, especially titanium, and review the concerns that have been raised about titanium materials in MRI.

In this study, co-infection of white spot syndrome virus (WSSV) and AHPND-Vibrio parahaemolyticus (VpAHPND), was studied in juvenile Penaeus vannamei under the laboratory condition. Experimental shrimp were divided into 4 groups (group 1: WSSV only; group 2: VpAHPND only; group 3: WSSV + VpAHPND; group 4: not challenged), and for the co-infection, shrimp were challenged with dual pathogens (initially exposed to WSSV and subsequently exposed to VpAHPND). During the experiments, we observed an increased mortality rate in the co-infection group (group 3, N = 7/20 shrimp at day 2 post-infection [p.i.], and N = 15/20 shrimp at day 3 p.i.) than other groups (N = 1/20 shrimp at day 3 p.i., and N = 10/20 shrimp at day 4 p.i. in group 2; no mortalities in group 1 and 4). Also, we found obvious differences in WSSV infection among groups; strong WSSV-positives were detected by immunohistochemistry examination (brown coloration) and qPCR assay (the copy numbers between 2.0 × 103–3.7 × 104) in the co-infection group on the termination day, but these were not detected in the other groups. In addition, there were prominent differences in the histopathology of the hepatopancreas of surviving shrimp among groups; shrimp of the co-infection group showed typical histopathology signs of AHPND in the hepatopancreas, with sloughing of hepatopancreatic tubule epithelial cells (G4) on the termination day, but the hepatopancreas in shrimp of the other groups appeared normal. This study demonstrated that shrimp initially exposed to WSSV allows VpAHPND to cause faster and higher mortality than shrimp infected with VpAHPND alone, and also leads to severe WSSV infection, indicating that WSSV is a risk factor for VpAHPND in ponds. Therefore, shrimp farmers should pay attention to the management of co-infection in the pond.

Comprehensive experimental works are carried out for the optimal design of a centrifugal blower adopted in an indoor unit of an air-conditioner. The models for consideration are typical multi-blade turbo blower and limit loaded one, respectively. The main interest lies on the fluid dynamics performance when the blower Is installed in the practical system. The methodologies are an experimental estimations with a wind tunnel for blower performance and PIV measurement for the detail flow information. A centrifugal blower with limit loaded fan shows pronounced performances in terms of the flow rate and static pressure rise and the reason is explained by the precise measurement of the flows between blades using PIV. Consequently, it is found that the blower is proper for the flows with a resistance in down stream such as a heat exchanger.

Using single cell gel electrophoresis, DNA single strand breaks were determined in various marine organisms. DNA damage on fish blood cells was detected to know whether there was a difference between Incheon, Pohang, Masan, and Tongyeong as a control site. Tongyeong showed the lowest DNA damage among the study areas. Mussels, transplanted to Masan Bay for one month, revealed high DNA damage at sites with high economical activity. In two weeks exposure of polychaete to Incheon sediments, higher DNA damage was detected in the sediment adjacent to Incheon harbor than open sea. These results suggested that the marine organism from the polluted area revealed a relatively high DNA damage. In addition, these areas might be contaminated with genotoxic compounds and comet assay was useful as a bioassay to detect DNA damage in marine organisms.

The morphological and histological changes of gonad in ridgetail prawn (Expalaemon carinicauda) were examined with the development of the external genitalia. The samples were collected monthly in Hampyung Bay on the western coast of Korea, from January to December 2003. The ovaries lie on the dorsal side of the female prawns thorax. The ovary cavity is located below the heart and above the hepatopancreas. The anterior part of the ovary is round and fused, and the posterior part has a pair of tubular structures. Testes are bilaterally symmetrical; the anterior part shows fused feature, and the posterior part consists of a pair of tubules. Based on histological examination, ripe eggs and spermatozoa were observed when mature females and males reach a length of about 14.9 and 11.5 mm CL, respectively. The immature female are almost same shape and structure with matured one in the endopod of first pleopod and appendix interna of the second pleopod, except the number of setae, and appear to be structurally complete after females reach a length of about 14.9 mm CL. The appendix masculina reaching beyond the distal end of the appendix interna of the second pleopod after males reach a length of more than 5.5 mm CL and a fully developed appendix masculina and appendix interna of the second pleopod appear up to a length of 11.5 mm CL.

Experiments were carried out to investigate Cd accumulation, elimination and cell response in juvenile rockfish (Sebastes schlegeli) exposed to sub-chronic dietary Cd (0, 0.5, 5, 25 and 125 mg/kg) for 60 days and depuration periods of 30 days. Cd accumulation in the kidney of cock fish increased with exposure periods and concentrations for the 60 days of dietary Cd exposure. After the end of the dietary Cd exposure, Cd accumulation values in the kidney were and for those exposed to 25 mg/kg and 125 mg/kg Cd, respectively. The accumulation factors increased with the exposure period in the kidney. Cd elimination in the kidney of rockfish did not vary significantly and remained constant after the cessation of the dietary Cd exposure. In the primary exposure periods, the effect of kidney tissue in the rockfish exposed to dietary Cd was observed the swelling of capillary of the glomerulus. In addition, there was also hydropic swelling within the pyknotic nuclei, some of hyaline droplet accumulation and the microvilli showed a positive reaction to alcian blue in the tubular cells. While exposure time and concentrations were increased, there was a lot of hyaline droplet accumulation and the microvilli showed a positive reaction to alcian blue in the tubular cells. Fused renal tubule and its necrosis were observed after 60 days at l25 mg/kg.

We examined the ovaries of 312 robust tonguefish (Cynoglossus robustus) collected each month from January to December 2001, off the coast of Yeosu, Korea. The samples were used to investigate robust tonguefish reproductive activity. Monthly changes in the gonadosomatic indexindicated that spawning peaked between June and August. Fish size at first spawning was 15-19 cm total length (TL). Over of female fish 25-29 cm TL were sexually mature, and this rose to for female fish 35-39 cm TL. The tonguefish spawned four or more times during the spawning season. Most females commenced spawning in their second year. The relationship between total length (cm TL) and fecundity (F) was F=528646Ln(TL)-1E+06.

Maturation and spawning of Acentrogobius pflaumi were studied using samples collected from the Gwangyang Bay, Korea, from January to December, 1999. The gonadosomatic index (GSI) of the female was the highest in May. The spawning season lasted from May to June. There was a significant difference in the 1 : 0.59 sex ratio between males and females (p for the size group between 4.5 cm and 4.9 cm, and for the size group over 5.5 cm in body length. The size of eggs spawned was between 0.4 to 0.6 mm, and the spawning occured once a year. Fecundity varied between 3,600 and 9,700 eggs. The relationship between the fecundity and body length of the fish was expressed in the fecundity equation as; $F

The in vitro effect of bisphenol A (BPA) on ovarian steroidogenesis of the longchin goby (Chasmichthys dolichognathus) was investigated. Oocytes taken during the maturing phase (vitellogenic, fully vitellogenic or germinal vesicle breakdown stage) were incubated with BPA (100 ng/mL) in the presence of exogenous precursor Steroids were extracted from the media and the isolated oocytes, and the extracts were separated and identified by thin layer chromatography and gas chromatography-mass spectrometry. The identities of the major metabolites were progestogens androgens [androstenedione (A4) and testosterone (T)] and estrogens [estrone BPA treatment inhibited production of estrogens in all the maturing phases and progestogens in the germinal vesicle migrating stage. Percentage yield of estrogens was decreased with increased yield of androgens. In conclusion, BPA had an inhibitory effect on the conversion of to estrogens and progestogens. These results demonstrate that BPA can act either estrogenic or anti-estrogenic effects.

Genital organs and gonad development in kishi velvet shrimp (Metapenaeopsis dalei) were studied based on morphological and histological observations. The samples were collected monthly around the Ochongdo area on the western coast of Korea, from April 1997 to December 1998. Thelycum appear structurally complete after females reach a length of about 12.0 mm CL. Petasma rarely Join after males reach a length of 10.6 mm CL and a fully developed petasma shows up to a length of 12.0 mm CL. The ovaries lie on the dorsal side of the female shrimps thorax. The ovary cavity is located below the heart and above the hepatopancreas. The anterior part of the ovary is round and fused, and the posterior part shows a pair of tubule-like structures. Testes are bilaterally symmetrical, the anterior part shows a convoluted tubule, and the posterior part consists of a pair of tubules. In histological observations, ripe eggs and spermatozoon show when mature females and males reach a length of about 12.0 mm CL.

Recently, digital watermarking has been proposed as a viable solution to the need of copyright protection and authentication of multimedia data. A robust wavelet-based watermark casting scheme and a watermark retrieval technique are suggested in this paper. We present a method which can add the watermark to the significant coefficients in the DWT domain, and does not require the original image in the detection process. In adaptive watermark casting method is developed to select perceptually significant coefficients for each subband using multiple threshold. In the proposed method, an adaptive multiple threshold scheme is used to reflect characteristics of each subband and complexity of image. The watermark is adaptively weighted in different subbands to achieve robustness as well as high perceptual quality. The watermark, Gaussian random sequence is added to the large coefficients but not in the lowest subband in the DWT domain. Experimental results show that the proposed algorithm produced visually very good watermarked image which has good invisibility to human eyes and very robust against various image processing and compression attacks.

The effect on kidney, gill, and hepatopancreas of juvenile olive flounder, Paralichthys olivaceus were investigated by histological method under limit concentration of effulent of PCBs for 60 days. The free surface of epithelial cell layer in the renal tubules of kidney showed a strong positive reaction in alcian blue during all exposure period. There were also observed swelling of hemocyte in glomerulus and macrophage. At 60 days after exposure, epithelium of the renal tubules was fused and some of organism showed damage of glomerulus and eosinophlic cell in epithelium of the renal tubules. The phenomena such as the activation and increase of the chloride cell, and swelling of hemocyte in the capillary of gill were observed during all exposure period. At 60 days after exposure, swelling of the gill filament and mucous cell appeared in gill and some of organism showed swelling of the gill lamellar. Swelling of hemocyte in the capillary of hepatopancreas was observed and the free surface of epithelial layer of the bile duct showed positive reaction in alcian blue during all exposure period. Degenerated zymogen in the pancreas and swelling of the hepatocyte were occurred at 60 days after exposure.

Experiments were carried out to investigate the accumulation and the histopathological changes in liver of juvenile rockfish, S. schlegeli, after sub-chronic dietary Cu (0, 50, 125, 250 and 500mg/kg) exposure for 60 days. Cu accumulation in liver was significantly increased with dietary exposure period and concentration for 60 days, and has a linear relation with dietary exposure days. After 60 days of Cu dietary exposure, the Cu concentration in the liver was dry weight and was approximately 11-fold, 18-fold and 51-fold higher than in the control diet group at 125, 250 and 500 mg/kg Cu diet group. The accumulation factors were increased with the dietary exposure period in liver of rockfish. In the primary exposed stage, the effect of hepatic tissue in the rockfish exposed to dietary Cu observed enlargement of hepatocytes nuclei, activity of hepatic cells and the swelling of hepatic cells. While exposed time and concentration were increased, the distinct granulation, irregular shape and necrosis of hepatic cells were observed. It was observed that granule degeneration and necrosis showed a part of cells in hepatic tissue after 60 days at 500 mg/kg.

Effect of PCBs on the skin and gill development of olive flounder, Paralichthys olivaceus were investigated by histological methodology and morphometric data. The olive flounder were exposed to limit concentration of effulent of PCBs (3.0 ) for 60 days. Skin development can be classified into four stages: SSEL (simple squamous epithelial layer), MCA (mucous cell appearance) & CCA (club cell appearance) DLA (dermal layer appearance), and SEL (startification of epidermal layer) stages. Gill development had five stages: GFA (gill filament appearance), IGFE (identification of early gill filament epithelial cell) MCA (mucous cell appearance), PCA (pillar cell appearance), and FGL (formation of gill lamella) stages. The periods of structural completion of skin and gill were 22-30 days and 23-30 days after hatching in the exposure group respectively. The process of development of skin and gill of the exposure group was very similar to that of the control group. Therefore, PCBs (3 ) have no influence on the development of skin and gill in the larval stage of olive flounder.

The authors conducted a survey on essential humanistic competency that medical students should have, and on teaching methods that will effectively develop such attributes. The participants consisted of 154 medical school professors, 589 medical students at Seoul National University College of Medicine, 228 parents, and 161 medical school and university hospital staff. They answered nine questions that the authors created. According to the results, all groups chose "morality and a sense of ethics," a "sense of accountability," "communication skills," and "empathic ability" were selected as essential qualities. According to the evaluation on the extent to which students possess each quality, participants believed students had a high "sense of accountability" and "morality," whereas they thought students had low "empathic ability," "communicate," or "collaborate with others". In terms of effective teaching methods, all sub-groups preferred extracurricular activities including small group activities, debates, and volunteer services. With regard to the speculated effect of humanism education and the awareness of the need for colleges to offer it, all sub-groups had a positive response. However the professors and students expressed a relatively passive stance on introducing humanism education as a credited course. Most participants responded that they preferred a grading method based on their rate of participation, not a relative evaluation. In order to reap more comprehensive and lasting effects of humanism education courses in medical school, it is necessary to conduct faculty training, and continuously strive to develop new teaching methods., Graphical Abstract

The viscous flow confined in a finite cylinder when one endwall disk and the sidewall are spinning about the central axis is studied. We examine the internal flow when a uniform axial suction through the spinning disk and a concomitant uniform radial inflow through the spinning sidewall are imposed. The rotational Reynolds number is large and the cylinder aspect ratio is O(l). Finite-difference techniques are employed to integrate numerically the full Navier-Stokes equations. The core rotation rate, which is uniform in the axial direction, increases as the suction increases. Under a sufficiently strong suction, the core rotation rate exceeds that of the spinning disk. The flows in the core away from the sidewall are depicted well by the predictions of the infinite disk model. A physical description based on an angular momentum argument is given. Due to the presence of the sidewall, the angular velocities in finite configuration vary in the radial direction, and this variation is pronounced under a strong suction. The meridional flow patterns are displayed. When the suction is weak, the bulk of the meridional fluid transport from the sidewall to the spinning disk takes route via the boundary layer near the stationary disk. Under a strong suction, the meridional fluid transport through the main body of the flow field increases. At small and moderate radii, the radial velocities nearly vanish in the core; the axial velocities increase in magnitude as the suction increases. When the suction is strong, the dynamic effects are concentrated in the Ekman layer near the spinning disk.

Pb-free Sn-based perovskite solar cells (PSCs) have significant potential for application in photovoltaic devices because oftheir suitable bandgap, low exciton binding energy, high carrier mobility, and long diffusion length. However, their performance is hampered by several issues. Sn-based perovskites are highly susceptible to oxidation, which induces a high concentration of defects and degrades the chemical stability of the perovskite crystals. Herein, the anion formate (HCOO-) can effectively suppress the oxidation of Sn in the pure formamidinium tin triiodide (FASnI(3)) perovskite without using A-site cationic additives. Moreover, the presence of formate results in a uniform pinhole-free perovskite film with a low trap density, reduced charge carrier recombination, and improved charge extraction. Density functional theory calculations show that formate-treated FASnI(3) has improved stability against oxidative Sn degradation. The formate-treated PSC achieves a power conversion efficiency of 12.11% at a high open-circuit voltage of 0.71 V. The device exhibits improved stability in ambient air in which it maintained over 80% of its initial power conversion efficiency after 180 min because oxidation is inhibited owing to the strong interaction between Sn and formate.