Search

Additional file 1: Fig. s1. Serum protein profiling of the BLM-induced SSc model treated with MT-7117. Serum samples from BLM-induced SSc mouse model (therapeutic model) treated with MT-7117 at 10 mg/kg or imatinib at 150 mg/kg from were used for serum protein profiling (all proteins that could be quantified). Using Luminex® assays, 110 proteins were investigated. The graphs show 67 proteins that were detectable in the quantitative range. All values are expressed as an individual plot dot and the mean ± SEM (n = 8). * p < 0.05, ** p < 0.01 by Student’s t-test (vs. BLM_vehicle group).

Additional file 5: Table s2. Microarray-based gene expression profiling of the BLM-induced SSc model treated with MT-7117.

Additional file 3: Fig. s3. Correlation between MC1R scores and mRSS in dcSSc patients. Staining intensity of MC1R in the skin of dcSSc patients (n = 50) was graded from 0 to 3. MC1R scores and mRSS of dcSSc patients were plotted, and the coefficient of determination (R2) was calculated. All values are expressed as an individual plot dot.

Additional file 4: Table s1. List of proteins measured in serum protein profiling.

Additional file 2: Fig. s2. Scores of melanocortin-1 receptor (MC1R) immunostaining in skin sections. The staining intensity of MC1R in skin samples from healthy subjects (n = 30), dcSSc patients (n = 50), and lcSSc (n = 10) patients was graded from 0 to 3. Qualitative assessment was performed by assigning a score based on staining intensity after identifying each stained cell type and tissue element. No staining, 0; faint staining, 0.5; light staining, 1; moderate staining, 2; dark staining: 3. All values are expressed as an individual plot dot and the mean ± SEM.

Tris-bipyridine ruthenium-complexes carrying a disialo complex-type oligosaccharide were prepared via a one-pot transglycosylation using endo-glycosidase (Endo M); they bind to type-A influenza viruses with excellent affinity (IC50 = 8.4 microM), and their luminescence intensity is strongly depressed by virus-binding.