George M. Church, Richard N. Hall, Benjamin W. Pruitt, Suhani Vora, Mohammad R. Ebrahimkhani, Dmitry Ter-Ovanesyan, Jacob Beal, Alejandro Chavez, Samira Kiani, Ron Weiss, Emma J. K. Kowal, James J. Collins, Marcelle Tuttle, Joanna Buchthal, Jason Qian, Raj Chari, MIT Synthetic Biology Center, Massachusetts Institute of Technology. Department of Biological Engineering, Kiani, Samira, Hall, Richard N., Beal, Jacob S, Vora, Suhani Deepak, Kowal, Emma J., Ebrahimkhani, Mohammad Reza, Collins, James J., and Weiss, Ron
We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein., National Human Genome Research Institute (U.S.) (P50 HG005550), United States. Department of Energy (DE-FG02-02ER63445), Wyss Institute for Biologically Inspired Engineering, United States. Army Research Office (DARPA W911NF-11-2-0054), National Science Foundation (U.S.), United States. National Institutes of Health (5R01CA155320-04), United States. National Institutes of Health (P50 GM098792), National Cancer Institute (U.S.) (5T32CA009216-34), Massachusetts Institute of Technology. Department of Biological Engineering, Harvard Medical School. Department of Genetics, Defense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006)