1. Oligodeoxyadenylate stimulates the protein kinase activity of anti-DNA sIgA from human milk
- Author
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Yuriy Kit, Kuligina, E., Semenov, D., and Richter, V.
- Subjects
Milk, Human ,food and beverages ,chemical and pharmacologic phenomena ,DNA ,Chromatography, Agarose ,digestive system ,Chromatography, DEAE-Cellulose ,General Biochemistry, Genetics and Molecular Biology ,Immunoglobulin A ,fluids and secretions ,Oligodeoxyribonucleotides ,stomatognathic system ,Humans ,Female ,Protein Kinases - Abstract
Preparations of anti-DNA sIgA were obtained from human milk by sequential chromatography on protein A-sepharose, DEAE-fractogel and DNA-cellulose. The influence of oligonucleotides on protein kinase activity was investigated. It was discovered that incubation of anti-DNA sIgA with oligodeoxyriboadenylate d(A)12 stimulates the phosphorylation of polypeptides of sIgA in the presence of [gamma-32P]ATP. The greatest was the incorporation of P into the sIgA H-chains. We also demonstrated stimulation of the casein kinase activity of anti-DNA sIgA by d(A)12. The stimulation of the protein kinase activity of anti-DNA sIgA by oligoriboadenylate r(A)12 was not detected.
- Published
- 2002
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