173 results on '"Le, Jia"'
Search Results
2. Stropharia rugoso-annulata acetylated polysaccharides alleviate NAFLD via Nrf2/JNK1/AMPK signaling pathways
- Author
-
Xueping, Li, Weijun, Cui, Yanfei, Cui, Xinling, Song, Le, Jia, and Jianjun, Zhang
- Subjects
NF-E2-Related Factor 2 ,General Medicine ,AMP-Activated Protein Kinases ,Diet, High-Fat ,Lipid Metabolism ,GA-Binding Protein Transcription Factor ,Biochemistry ,Mice, Inbred C57BL ,Mice ,Liver ,Non-alcoholic Fatty Liver Disease ,Polysaccharides ,Structural Biology ,Animals ,Mitogen-Activated Protein Kinase 8 ,Agaricales ,Molecular Biology ,Signal Transduction - Abstract
The acetylated Stropharia rugoso-annulata polysaccharides (ASRP) was successfully characterized, and the effects and mechanism on alleviating NAFLD were investigated in HFD-induced mice models. The characterization showed that ASRP was successfully acetylated and rich in galactose. The animal studies demonstrated that ASRP at the dose of 400 mg/kg possessed hepatoprotective effects by potential antioxidation, anti-inflammation and improving hepatocellular histopathology, with the possible mechanisms on regulating the JNK1/AP-1 and activating the Nrf2 signaling pathways. Besides, ASRP could improve the fat metabolism by activating the AMPK/SREBP-1c signaling pathways. The results provided basal theories for the development of ASRP on treating the NAFLD and its complications.
- Published
- 2022
3. Tunable synthesis of furfurylamines or β-amino alcohols via Ru-catalyzed N–H functionalization using biomass-derived polyols
- Author
-
Le Jia, Xue Wang, Xiaolong Gao, Mohamed Makha, Xi-Cun Wang, and Yuehui Li
- Subjects
General Medicine - Published
- 2022
4. A new genus of river snails, Dalipaludina (Gastropoda, Viviparidae), endemic to the Yunnan Plateau of SW China
- Author
-
Le-Jia Zhang, Li-Na Du, and Thomas von Rintelen
- Subjects
taxonomy ,lentic habitat ,Mollusca ,Gastropoda ,Caenogastropoda ,High altitude ,Animalia ,Viviparidae ,Architaenioglossa ,Viviparoidea ,phylogeny ,Biota ,Ecology, Evolution, Behavior and Systematics - Abstract
A new genus of viviparid snail, Dalipaludinagen. nov., from the Yunnan Plateau of China is described within an integrative taxonomic framework based on data from the mitochondrial COI marker and morphology. Dalipaludina can be distinguished from all other viviparid genera by a unique combination shell, operculum and radula characters. Four species are assigned here to the new genus, Dalipaludina delavayanacomb. nov., Dalipaludina oxytropoidescomb. nov., Dalipaludina occidentaliscomb. nov., and Dalipaludina pyramidellacomb. nov., and one species is newly assigned to Margarya, Margarya dianchiensiscomb. nov. The four species of Dalipaludina are allopatrically distributed in shallow water lentic habitats at high altitude regions of the Yunnan Plateau.
- Published
- 2023
5. Structure Analysis, Antioxidant Activity, Anti‐Aging, and Organ Protective Effects of Pholiota adiposa Residue Polysaccharides
- Author
-
Minjie Hou, Xiaoxu Li, Xueping Li, Jianjun Zhang, and Le Jia
- Subjects
Organic Chemistry ,Food Science - Published
- 2023
6. Fast In-Situ Triaxial Remanent Magnetic Field Measurement for Single-Beam SERF Atomic Magnetometer Based on Trisection Algorithm
- Author
-
Tengyue Long, Bangcheng Han, Xinda Song, Yuchen Suo, and Le Jia
- Subjects
Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials - Abstract
We demonstrate a method for quickly and automatically detecting all three components of a remanent magnetic field around a shielded spin-exchange relaxation-free (SERF) atomic magnetometer (AM) using the trisection algorithm (TSA) for zero-field resonance (ZFR). To satisfy the measurement of AMs, a resonance light of the 87Rb D1 line with a spectral width of less than 1MHz is converted to circular polarization by a linear polarizer and a quarter-wave plate. After the light beam has passed through the alkali metal vapor cell, the residual magnetic field can be measured by searching for triaxial ZFR optical peaks. The TSA stably reduces the measurement time to 2.41 s on average and improves the measurement accuracy, significantly outpacing existing methods. The weighted averages of all measurements with corresponding uncertainties are (−15.437 ± 0.022)nT, (6.062 ± 0.021)nT, and (−14.158 ± 0.052)nT on the x-, y-, and z-axes, respectively. These improvements could facilitate more extremely weak magnetic studies in real time, such as magnetoencephalography (MEG) and magnetocardiography (MCG) measurements.
- Published
- 2023
7. Machining Feature Recognition Method Based on Improved Mesh Neural Network
- Author
-
Jia-Le Jia, Sheng-Wen Zhang, You-Ren Cao, Xiao-Long Qi, and null WeZhu
- Subjects
Mechanics of Materials ,Mechanical Engineering ,Computational Mechanics - Published
- 2023
8. Cleavage∕cross-coupling strategy for converting β-O-4 linkage lignin model compounds into high valued benzyl amines via dual C–O bond cleavage
- Author
-
Huiying Zeng, Chao-Jun Li, and Le Jia
- Subjects
Chemistry ,fungi ,technology, industry, and agriculture ,food and beverages ,Lignocellulosic biomass ,Biomass ,macromolecular substances ,General Chemistry ,Cleavage (embryo) ,complex mixtures ,chemistry.chemical_compound ,Organic chemistry ,Lignin ,Degradation (geology) ,Amine gas treating ,Phenols ,Bond cleavage - Abstract
Lignin is the most recalcitrant of the three components of lignocellulosic biomass. The strength and stability of the linkages have long been a great challenge for the degradation and valorization of lignin biomass to obtain bio-fuels and commercial chemicals. Up to now, the selective cleavage of C–O linkages of lignin to afford chemicals contains only C, H and O atoms. Our group has developed a cleavage/cross-coupling strategy for converting 4-O-5 linkage lignin model compounds into high value-added compounds. Herein, we present a palladium-catalyzed cleavage/cross-coupling of the β-O-4 lignin model compounds with amines via dual C–O bond cleavage for the preparation of benzyl amine compounds and phenols.
- Published
- 2022
9. Glucopyranose from Pleurotus geesteranus prevent alcoholic liver diseases by regulating Nrf2/HO-1-TLR4/NF-κB signalling pathways and gut microbiota
- Author
-
Xinling Song, Weijun Cui, Fanyun Meng, Qun Xia, Xueping Li, Minjie Hou, Le Jia, and Jianjun Zhang
- Subjects
General Medicine ,Food Science - Abstract
PGPs from Pleurotus geesteranus enhanced hepatic functions by improving oxidative status via up-regulating Nrf2/HO-1 signalling pathways, and decreasing inflammation via down-regulating TLR4/NF-κB signalling pathways.
- Published
- 2022
10. Coprinus comatus polysaccharides ameliorated carbon tetrachloride-induced liver fibrosis through modulating inflammation and apoptosis
- Author
-
Huajie Zhao, Duan Li, Min Li, Liang Liu, Baoguo Deng, Le Jia, and Fan Yang
- Subjects
General Medicine ,Food Science - Abstract
Liver fibrosis is a serious global public health problem, owing to a lack of effective treatment.
- Published
- 2022
11. Mycelium polysaccharides of Macrolepiota procera alleviate reproductive impairments induced by nonylphenol
- Author
-
Wenshuai Wang, Xiaoxu Li, Yaohan Zhang, Jianjun Zhang, and Le Jia
- Subjects
General Medicine ,Food Science - Abstract
Mycelium polysaccharides of Macrolepiota procera (MMP) had positive role in amelioration of NP-induced reproductive disorders.
- Published
- 2022
12. Effect of nimodipine combined with atorvastatin calcium on microinflammation and oxidative stress levels in patients with cerebral vasospasm after subarachnoid hemorrhage
- Author
-
Ning Gan, Tong-le Jia, Nan Tian, Si-si Liu, and Shan Cao
- Subjects
General Medicine - Abstract
Objective: To evaluate the effect of nimodipine combined with atorvastatin calcium on the micro inflammation and oxidative stress levels in patients with cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH) and its clinical implications. Methods: A total of 80 patients with CVS caused by SAH who had been admitted to Baoding First Central Hospital from August 2021 to August 2022 were selected and randomly divided into two groups. The control group underwent conventional symptomatic treatment, while the experimental group was administered nimodipine combined with atorvastatin calcium on the basis of conventional treatment. The changes in the micro inflammatory cytokines and oxidative stress factors in the two groups were compared, as well as the differences in clinical efficacy and incidence of adverse drug reactions. Results: After treatment, the levels of inflammatory cytokines in the experimental group decreased more significantly than those in the control group (p=0.00). After treatment, the serum levels of oxidative stress factors were obviously higher in the experimental group than in the control group (p=0.00). After treatment, the total efficacy was 77.5% in the experimental group and 55% in the control group, and the difference was statistically significant (p=0.04). Conclusions: Nimodipine combined with atorvastatin calcium could significantly improve the clinical symptoms in patients with CVS after SAH, which would be beneficial, safe, and effective for the patient’s recovery. doi: https://doi.org/10.12669/pjms.39.2.6721 How to cite this: Gan N, Jia T, Tian N, Liu S, Cao S. Effect of nimodipine combined with atorvastatin calcium on microinflammation and oxidative stress levels in patients with cerebral vasospasm after subarachnoid hemorrhage. Pak J Med Sci. 2023;39(2):---------. doi: https://doi.org/10.12669/pjms.39.2.6721 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Published
- 2023
13. DJ-1 activates the noncanonical NF-κB pathway via interaction with Cezanne to inhibit the apoptosis and promote the proliferation of Ishikawa cells
- Author
-
Xue-Ying Wang, Zhong-Qing Xiao, Ting-Ting Zhou, He-Ping Chen, Qi-Zhou Zhu, Xiao-Yan Zhao, Le-Jia Qiu, and Hao-Yue Liu
- Subjects
Cell Survival ,Protein Deglycase DJ-1 ,Gene Expression ,Apoptosis ,Flow cytometry ,chemistry.chemical_compound ,Cell Line, Tumor ,Endopeptidases ,Genetics ,medicine ,Humans ,Viability assay ,Molecular Biology ,Cell Proliferation ,Gene knockdown ,medicine.diagnostic_test ,Chemistry ,Cell growth ,NF-kappa B ,NF-κB ,General Medicine ,female genital diseases and pregnancy complications ,Endometrial Neoplasms ,Cell biology ,Gene Expression Regulation, Neoplastic ,Blot ,Cell culture ,Female ,Signal Transduction - Abstract
Endometrial cancer is generally one of the most evident malignant tumours of the female reproductive system, and the mechanisms underlying its cell proliferation and apoptosis are key to research in gynaecological oncology. In the paper, the in-depth molecular mechanism by which DJ-1 protein regulates the proliferation and apoptosis of Ishikawa cells was investigated. DJ-1 knockdown and overexpressing Ishikawa stable cell lines were established by lentiviral transduction. The levels of DJ-1 and noncanonical NF-κB signaling proteins were evaluated by Western blotting. Cell counting kit-8 (CCK-8) and flow cytometry were applied to analyze the cell viability and apoptosis. Co-immunoprecipitation experiment was utilized to assess the DJ-1-Cezanne interaction. The results showed that DJ-1 overexpression conferred apoptosis resistance and high proliferation on Ishikawa cells, while DJ-1 knockdown in Ishikawa cells produced the opposite results. These findings again suggested that DJ-1 inhibits the apoptosis and promotes the proliferation of Ishikawa cells. More crucially, further data showed that the noncanonical NF-κB activation was required for the regulation of Ishikawa cell proliferation and apoptosis by DJ-1. Meanwhile, it was found that noncanonical NF-κB pathway may be activated by DJ-1 interacting with and negatively regulating Cezanne in Ishikawa cells. Overall, this work revealed that DJ-1 associates with and negatively regulates Cezanne and consequently triggers the noncanonical NF-κB activation, thereby regulating Ishikawa cell proliferation and apoptosis.
- Published
- 2021
14. Polysaccharides from Oudemansiella radicata residues attenuate carbon tetrachloride-induced liver injury
- Author
-
Wenshuai Wang, Yaohan Zhang, Xinchao Liu, Zonghui Liu, Le Jia, and Jianjun Zhang
- Subjects
Structural Biology ,General Medicine ,Molecular Biology ,Biochemistry - Published
- 2023
15. Ganoderma lucidum polysaccharides improve lipid metabolism against high-fat diet-induced dyslipidemia
- Author
-
Wenshuai Wang, Yaohan Zhang, Zhiying Wang, Jianjun Zhang, and Le Jia
- Subjects
Pharmacology ,Drug Discovery - Published
- 2023
16. Suppression of modulation-magnetic-fields crosstalk for single-beam optically-pumped magnetometers
- Author
-
Yuchen Suo, Xinda Song, Liwei Jiang, Le Jia, Tengyue Long, and Zhendong Wu
- Subjects
Applied Mathematics ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation - Published
- 2023
17. Rapid diagnosis of disseminated Mycobacterium mucogenicum infection in formalin-fixed, paraffin-embedded specimen using next-generation sequencing: A case report
- Author
-
Chao Zhuo, Jing Liu, Xiao-Hua Yang, Zhiliang Gao, Ying-Xiong Huang, Le-Jia Xu, Yi-Hua Pang, Bingliang Lin, Jianyun Zhu, Ziying Lei, and Jia-Xing Zheng
- Subjects
Formalin-fixed, paraffin-embedded specimen ,Formalin fixed paraffin embedded ,biology ,business.industry ,Disseminated infection ,Rapid diagnosis ,General Medicine ,biology.organism_classification ,Virology ,DNA sequencing ,Case report ,Mycobacterium mucogenicum ,Next-generation sequencing ,Medicine ,business - Abstract
BACKGROUND Mycobacterium mucogenicum (M. mucogenicum) belongs to the group of rapidly growing Nontuberculous mycobacteria. This microorganism is associated with a wide spectrum of infectious diseases. Due to a low detection rate or the time required for conventional culture methodology, a rapid and broad-spectrum method is necessary to identify rare pathogens. CASE SUMMARY A 12-year-old immunocompetent girl presented with painful masses for five months. The first mass was found in the right upper quadrant of the abdomen, and was about 1 cm × 1.5 cm in size, tough but pliable in texture, with an irregular margin and tenderness. An abscess gradually formed and ulcerated with suppuration of the mass. Three new masses appeared on the back one by one. Chest computed tomography showed patchy and streaky cloudy opacities in both lungs. Needle aspiration of the abscess was performed, but the smear and conventional culture were negative, and the pathological examination showed no pathogens. We then performed next-generation sequencing using a formalin-fixed, paraffin-embedded specimen to identify the pathogen. A significantly high abundance of M. mucogenicum was detected. The patient’s abscesses gradually decreased in size, while inflammation in both lungs improved following 12-wk of treatment. No recurrence was observed four months after the end of the one-year treatment period. CONCLUSION Next-generation sequencing is a promising tool for the rapid and accurate diagnosis of rare pathogens, even when using a formalin-fixed, paraffin-embedded specimen.
- Published
- 2021
18. Transformable Nanosensitizer with Tumor Microenvironment‐Activated Sonodynamic Process and Calcium Release for Enhanced Cancer Immunotherapy
- Author
-
Jingzhao Huang, Yanhong Zhu, Le Jia, Kanyi Pu, Xiangliang Yang, Shasha He, Yiqian Wang, Yan Zhang, Xuan Tan, and School of Chemical and Biomedical Engineering
- Subjects
medicine.medical_treatment ,Ultrasonic Therapy ,Breast Neoplasms ,010402 general chemistry ,01 natural sciences ,Catalysis ,Mice ,Cancer immunotherapy ,Cell Line, Tumor ,medicine ,Tumor Microenvironment ,Animals ,Particle Size ,chemistry.chemical_classification ,Titanium ,Reactive oxygen species ,Tumor microenvironment ,010405 organic chemistry ,Chemistry ,Cancer Sono-Immunotherapy ,Sonodynamic therapy ,Chemical engineering [Engineering] ,General Chemistry ,General Medicine ,Immune Checkpoint Blockade ,0104 chemical sciences ,Blockade ,Toxicity ,Cancer research ,Immunogenic cell death ,Nanoparticles ,Calcium ,Female ,Intracellular - Abstract
Despite the promise of sonodynamic processes in cancer therapy, existing sonosensitizers often fail to regulate the generation of reactive oxygen species (ROS) against tumors, potentially leading to off-target toxicity to normal tissues. We report a transformable core-shell nanosonosensitizer (TiO2 @CaP) that reinvigorates ROS generation and dissolves its CaP shell to release Ca2+ in an acidic tumor microenvironment (TME) under ultrasound activation. Thus, TiO2 @CaP acts as a smart nanosonosensitizer that specifically induces mitochondrial dysfunction via overloading intracellular Ca2+ ions to synergize with the sonodynamic process in the TME. TiO2 @CaP substantially enhances immunogenic cell death, resulting in enhanced T-cell recruitment and infiltration into the immunogenic cold tumor (4T1). In conjunction with checkpoint blockade therapy (anti-PD 1), TiO2 @CaP-mediated sonodynamic therapy elicits systemic antitumor immunity, leading to regression of non-treated distant tumors and inhibition of lung metastasis. This work paves the way to development of "smart" TME-activatable sonosensitizers with temporospatial control over antitumor responses. Agency for Science, Technology and Research (A*STAR) Ministry of Education (MOE) We thank the National Key R&D Program of China (2020YFA0710700), National Natural Science Foundation of China(81627901, 81573013, 81703031, and81773653) and opening foundation of Hubei key laboratory of molecular imaging (2020fzyx025) for financial support. We also thank the Analytical and Testing Centre of Huazhong University of Science and Technology for related analysis.K.P.thanks the Ministry of Education Singapore, Academic Research Fund Tier 1 (2019-T1-002-045 RG125/19 and RT05/20) Academic Research Fund Tier 2 (MOE2018-T2-2-042), and A*STAR SERC AME Programmatic Fund (SERC A18A8b0059) for the financial support.
- Published
- 2021
19. miR-99a-5p: A Potential New Therapy for Atherosclerosis by Targeting mTOR and Then Inhibiting NLRP3 Inflammasome Activation and Promoting Macrophage Autophagy
- Author
-
Gang Wang, Shu-Yan Jing, Gang Liu, Xue-Jia Guo, Wei Zhao, Xin-Le Jia, Ming-Qi Zheng, and Wen-Yun Tan
- Subjects
Article Subject ,Inflammasomes ,Macrophages ,TOR Serine-Threonine Kinases ,Biochemistry (medical) ,Clinical Biochemistry ,General Medicine ,Atherosclerosis ,Lipoproteins, LDL ,Mice ,MicroRNAs ,NLR Family, Pyrin Domain-Containing 3 Protein ,Genetics ,Autophagy ,Animals ,Molecular Biology - Abstract
Objective. MicroRNAs have been revealed to be involved in the development of atherosclerosis. The present study is aimed at exploring the potential of miR-99a-5p as a therapy for atherosclerosis. We suspected that miR-99a-5p might inhibit NLRP3 inflammasome activation and promote macrophage autophagy via constraining mTOR, therefore, alleviating atherosclerosis. Methods. The cell viability in ox-LDL-induced THP-1 macrophages was assessed by CCK-8 assay. Bioinformatic analysis was used to predict the target genes of miR-99a-5p. The binding between miR-99a-5p and mTOR was confirmed by luciferase reporter assay. In vivo, a high-fat-diet-induced atherosclerosis model was established in apolipoprotein E knockout mice. Hematoxylin-eosin, oil red O, and Sirius red staining were performed for the determination of atherosclerotic lesions. MTOR and associated protein levels were detected by Western blot analysis. Results. miR-99a-5p inhibited NLRP3 inflammasome activation and promoted macrophage autophagy by targeting mTOR. Enforced miR-99a-5p significantly reduced the levels of inflammasome complex and inflammatory cytokines. Furthermore, miR-99a-5p overexpression inhibited the expression of mTOR, whereas mTOR overexpression reversed the trend of the above behaviors. In vivo, the specific overexpression of miR-99a-5p significantly reduced atherosclerotic lesions, accompanied by a significant downregulation of autophagy marker CD68 protein expression. Conclusion. We demonstrated for the first time that miR-99a-5p may be considered a therapy for atherosclerosis. The present study has revealed that miR-99a-5p might inhibit NLRP3 inflammasome activation and promote macrophage autophagy by targeting mTOR, therefore, alleviating atherosclerosis.
- Published
- 2022
20. Mycelium polysaccharides of
- Author
-
Wenshuai, Wang, Xiaoxu, Li, Yaohan, Zhang, Jianjun, Zhang, and Le, Jia
- Subjects
Male ,Mice ,Oxidative Stress ,Mycelium ,Phenols ,Polysaccharides ,Testis ,Animals ,Apoptosis ,Agaricales - Abstract
Nonylphenol (NP) exposure has become a crucial inducement of male reproductive disorders in the world. Therefore, it is urgent to seek solutions to alleviate the toxicity of NP. This study was oriented toward studying the protective effects of
- Published
- 2022
21. Characterization and Hepatoprotections of Ganoderma lucidum Polysaccharides against Multiple Organ Dysfunction Syndrome in Mice
- Author
-
Yiwen Zhang, Yanbo Feng, Wenshuai Wang, Le Jia, and Jianjun Zhang
- Subjects
Male ,0301 basic medicine ,Aging ,Reishi ,Antioxidant ,medicine.medical_treatment ,02 engineering and technology ,Pharmacology ,Biochemistry ,Antioxidants ,Mice ,chemistry.chemical_compound ,Malondialdehyde ,Toxicity Tests, Chronic ,Lipid peroxide ,Alanine Transaminase ,Syndrome ,General Medicine ,Catalase ,021001 nanoscience & nanotechnology ,Liver ,Cytokines ,Inflammation Mediators ,0210 nano-technology ,Research Article ,endocrine system ,Article Subject ,Multiple Organ Failure ,Intraperitoneal injection ,Oxidative phosphorylation ,Protective Agents ,Dinoprostone ,03 medical and health sciences ,Polysaccharides ,In vivo ,Toxicity Tests, Acute ,medicine ,Animals ,Aspartate Aminotransferases ,QH573-671 ,Superoxide Dismutase ,business.industry ,Zymosan ,Cell Biology ,Alkaline Phosphatase ,medicine.disease ,030104 developmental biology ,Hepatoprotection ,chemistry ,Cyclooxygenase 2 ,Cytology ,Multiple organ dysfunction syndrome ,business - Abstract
Background. The liver is one of the most commonly affected organs in multiple organ dysfunction syndrome (MODS). In recent years, there have been many studies on Ganoderma lucidum polysaccharides (GLP), but the role of GLP in MODS is still unclear. The purpose of this work was to explore the antioxidant, anti-inflammatory, and protective effects of GLP on the liver in MODS model mice. Methods. The characteristic properties of GLP were processed by physicochemical analysis. The MODS models were successfully established with intraperitoneal injection of zymosan in Kunming strain mice. The antioxidant, anti-inflammatory, and hepatoprotective effects of GLP were processed both in vitro and in vivo by evaluating the oxidative parameters, inflammatory factors, and liver pathological observations. Results. The characterization analysis revealed that GLP was a pyranose mainly composed of glucose with the molecular weights (Mw) of 8309 Da. The experimental results proved that GLP had potential hepatoprotection possibly by improving the antioxidant status (scavenging excessive oxygen radicals, increasing the antioxidant enzyme activities, and reducing the lipid peroxide), alleviating the inflammatory response (reducing the inflammatory factor levels), and guaranteeing the liver functions. Conclusions. This research suggested that GLP had the potential to be developed as a natural medicine for the treatment of multiple organ failure.
- Published
- 2021
22. Antioxidation, anti-inflammation and anti-fibrosis effect of phosphorylated polysaccharides from Pleurotus djamor mycelia on adenine-induced chronic renal failure mice
- Author
-
Huaping Li, Yanbo Feng, Le Jia, Wenxue Sun, and Yi Kong
- Subjects
Antioxidant ,medicine.medical_treatment ,Anti-Inflammatory Agents ,Pleurotus djamor ,02 engineering and technology ,Pharmacology ,Kidney ,Pleurotus ,Polysaccharide ,Biochemistry ,High-performance liquid chromatography ,Antioxidants ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Functional Food ,Structural Biology ,medicine ,Animals ,Phosphorylation ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Mycelium ,biology ,Adenine ,Fungal Polysaccharides ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Phosphate ,Blot ,Disease Models, Animal ,Oxidative Stress ,medicine.anatomical_structure ,chemistry ,Dietary Supplements ,Kidney Failure, Chronic ,Immunohistochemistry ,0210 nano-technology - Abstract
The mycelia polysaccharides (MPS) from Pleurotus djamor were prepared and purified by anion exchange column chromatography, and the phosphate content of phosphorylated MPS (PMPS) was 15.22 ± 0.37%. FT-IR spectra, HPLC and 1H and 13C-NMR results showed the PMPS contained α-pyranose structure and the peak area percentage composition of galacturonic acid and glucose were 13.01% and 85.82%, respectively. Animal experiment investigated the antioxidant, anti-inflammation, anti-fibrosis effects of PMPS on kidney in adenine-induced chronic renal failure (CRF) mice. All results including serum biochemical indices, histopathological observation, qRT-PCR, western blotting, immunohistochemical staining manifested the kidney injury could be remitted by PMPS interventions. This experiment suggested that PMPS could remit CRF and other kidney injury related diseases as one kind of dietary supplements and functional foods without toxic side effects.
- Published
- 2021
23. Cordyceps militaris polysaccharide alleviates diabetic symptoms by regulating gut microbiota against TLR4/NF-κB pathway
- Author
-
Huajie Zhao, Min Li, Liang Liu, Duan Li, Linjing Zhao, Zhen Wu, Mingxu Zhou, Le Jia, and Fan Yang
- Subjects
Structural Biology ,General Medicine ,Molecular Biology ,Biochemistry - Published
- 2023
24. Magnetic field interference suppression for minimized SERF atomic magnetometer
- Author
-
Le Jia, Xinda Song, Yuchen Suo, Jianli Li, Tengyue Long, and Xiaolin Ning
- Subjects
Metals and Alloys ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Instrumentation ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
25. A polysaccharide of PFP-1 from Pleurotus geesteranus attenuates alcoholic liver diseases via Nrf2 and NF-κB signaling pathways
- Author
-
Wenxue Sun, Jianjun Zhang, Xinling Song, Weijun Cui, and Le Jia
- Subjects
Male ,0301 basic medicine ,Alcoholic liver disease ,NF-E2-Related Factor 2 ,02 engineering and technology ,Pharmacology ,Pleurotus ,medicine.disease_cause ,Polysaccharide ,Antioxidants ,Mice ,03 medical and health sciences ,medicine ,Animals ,Liver Diseases, Alcoholic ,Liver injury ,chemistry.chemical_classification ,biology ,Molecular mass ,Chemistry ,NF-kappa B ,Fungal Polysaccharides ,General Medicine ,021001 nanoscience & nanotechnology ,medicine.disease ,biology.organism_classification ,Toll-Like Receptor 4 ,Nf κb signaling ,Oxidative Stress ,030104 developmental biology ,Liver ,Animal studies ,0210 nano-technology ,Oxidative stress ,Signal Transduction ,Food Science - Abstract
A polysaccharide named PFP-1 was isolated from the Pleurotus geesteranus fruiting body, and potential investigations on ameliorating oxidative stress and liver injury against alcoholic liver disease (ALD) were performed in mice. The animal studies demonstrated that PFP-1 had hepatoprotective effects by improving hepatocellular histopathology, modulating alcohol metabolisms and restoring the serum lipid levels. Besides, PFP-1 could attenuate oxidative stress and inflammatory responses by activating the Nrf2-mediated signal pathways and regulating the TLR4-mediated NF-κB signal pathways. The characterization indicated that PFP-1 was a typical pyranose-polysaccharide in a triple-helical conformation, which was linked by t-β-Glcp, 1,6-α-Glcp and 1,2,6-α-Galp. And the characteristic properties of higher water solubility and appropriate molecular weights contributed to the superior bioactivities. The results demonstrated that PFP-1 could be used as a supplement for alleviating alcohol-induced liver damage.
- Published
- 2021
26. Environmental eustress improves postinfarction cardiac repair via enhancing cardiac macrophage survival
- Author
-
Pei-Yuan Bai, Si-Qin Chen, Dai-Le Jia, Li-Hong Pan, Chao-Bao Liu, Jin Liu, Wei Luo, Yang Yang, Ma-Yu Sun, Nai-Fu Wan, Wu-Wei Rong, Ai-Jun Sun, and Jun-Bo Ge
- Subjects
Mice ,Multidisciplinary ,Ventricular Remodeling ,Brain-Derived Neurotrophic Factor ,Macrophages ,Myocardium ,Myocardial Infarction ,Animals ,Heart - Abstract
Macrophages play a vital role in cardiac repair following myocardial infarction (MI). An enriched environment (EE) is involved in the regulation of macrophage-related activities and disease progression; however, whether EE affects the phenotype and function of macrophages to improve postinfarction cardiac repair remains unknown. In this study, we found that EE improved cardiac function, decreased mortality, and ameliorated adverse ventricular remodeling in mice after MI, with these outcomes closely related to the increased survival of Ly6C low macrophages and their CCR2 − MHCII low subsets. EE increased the expression of brain-derived neurotrophic factor (BDNF) in the hypothalamus, leading to higher circulating levels of BDNF, which, in turn, regulated the cardiac macrophages. BDNF bound to tropomyosin receptor kinase B to activate downstream ERK1/2 and AKT pathways, promoting macrophage survival. These findings demonstrate that EE optimizes postinfarction cardiac repair and highlights the significance of EE as a previously unidentified strategy for impeding adverse ventricular remodeling.
- Published
- 2022
27. DJ-1 is involved in the multidrug resistance of SGC7901 gastric cancer cells through PTEN/PI3K/Akt/Nrf2 pathway
- Author
-
Yi-Zhang Deng, He-Ping Chen, Lin Xiao, Zhengming Zhu, Xiao-Ran Li, Xing-Wang Xu, Zhao-Xia Ma, Guang-Ling Duan, Le-Jia Qiu, Hao-Yue Liu, and Le Zhao
- Subjects
0301 basic medicine ,NF-E2-Related Factor 2 ,Morpholines ,Protein Deglycase DJ-1 ,Biophysics ,Biochemistry ,Phosphatidylinositol 3-Kinases ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Cell Line, Tumor ,medicine ,Humans ,PTEN ,ATP Binding Cassette Transporter, Subfamily B, Member 1 ,Phosphorylation ,PI3K/AKT/mTOR pathway ,P-glycoprotein ,Kelch-Like ECH-Associated Protein 1 ,Phosphoinositide 3-kinase ,biology ,Akt/PKB signaling pathway ,PTEN Phosphohydrolase ,Cancer ,General Medicine ,medicine.disease ,Drug Resistance, Multiple ,Up-Regulation ,Multiple drug resistance ,030104 developmental biology ,Proto-Oncogene Proteins c-bcl-2 ,Chromones ,Doxorubicin ,Drug Resistance, Neoplasm ,Vincristine ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Cancer cell ,biology.protein ,Cancer research ,Fluorouracil ,Cisplatin ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Gastric cancer is a common malignancy worldwide. The occurrence of multidrug resistance (MDR) is the major obstacle for effective gastric cancer chemotherapy. In this study, the in-depth molecular mechanism of the DJ-1-induced MDR in SGC7901 gastric cancer cells was investigated. The results showed that DJ-1 expression level was higher in MDR variant SGC7901/VCR cells than that in its parental SGC7901 cells. Moreover, DJ-1 overexpression conferred the MDR phenotype to SGC7901 cells, while DJ-1 knockdown in SGC7901/VCR cells induced re-sensitization to adriamycin, vincristine, cisplatin, and 5-fluorouracil. These results suggested that DJ-1 mediated the development of MDR in SGC7901 gastric cancer cells. Importantly, further data revealed that the activation of PI3k/Akt and Nrf2 signaling pathway were required for the DJ-1-induced MDR phenotype in SGC7901 gastric cancer cells. Meanwhile, we found that PI3k/Akt pathway was activated probably through DJ-1 directly binding to and negatively regulating PTEN, consequently resulting in Nrf2 phosphorylation and activation, and thereby inducing Nrf2-dependent P-glycoprotein (P-gp) and Bcl-2 expressions in the DJ-1-mediated MDR of SGC7901 gastric cancer cells. Overall, these results revealed that activating PTEN/PI3K/Akt/Nrf2 pathway and subsequently upregulating P-gp and Bcl-2 expression could be a critical mechanism by which DJ-1 mediates the development of MDR in SGC7901 gastric cancer cells. The new findings may be helpful for understanding the mechanisms of MDR in gastric cancer cells, prompting its further investigation as a molecular target to overcome MDR.
- Published
- 2020
28. Antioxidant and hepatoprotective effects against acute CCl 4 ‐induced liver damage in mice from red‐fleshed apple flesh flavonoid extract
- Author
-
Zhangwen Guo, Zheng Gao, Zongying Zhang, Le Jia, Xuesen Chen, Zhang Tianliang, Xu Haifeng, Wang Nan, Guanxian Yang, and Qi Zou
- Subjects
Malus ,Antioxidant ,030309 nutrition & dietetics ,medicine.medical_treatment ,Flavonoid ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,0404 agricultural biotechnology ,medicine ,Food science ,chemistry.chemical_classification ,0303 health sciences ,biology ,Glutathione peroxidase ,fungi ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,Malondialdehyde ,040401 food science ,chemistry ,Catalase ,Anthocyanin ,biology.protein ,Food Science - Abstract
Apple (Malus domestica Borkh.) is an important fruit tree species worldwide. Apple fruits are favored by consumers because of their antioxidative, anti-inflammatory, and antitumor effects as well as their protective effects against cardiovascular diseases and other health benefits. There is considerable interest in red-fleshed apple fruits among breeders because of their high flavonoid and anthocyanin contents. However, the flavonoids extracted from red-fleshed apple fruits must still be functionally characterized, especially regarding their protective effects against certain pathologies. In this study, the flavonoid components and contents in the extracts prepared from red-fleshed apple cultivar "Meihong" were determined. Additionally, the in vitro antioxidant activities and protective effects of the extracts against CCl4 -induced acute liver injury were investigated. The red-fleshed apple flesh flavonoid extract (RAFF) exhibited strong in vitro antioxidant activities. Compared with the model control mice treated with CCl4 , the mice pretreated with high (800 mg/kg·bw), middle (400 mg/kg·bw), and low (200 mg/kg·bw) RAFF doses had significantly lower CCl4 -induced serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase activities. Moreover, the RAFF pretreatment also significantly decreased the liver malondialdehyde activity and prevented the CCl4 -induced decrease in liver superoxide dismutase, glutathione peroxidase, catalase, and reduced glutathione levels. Furthermore, a histopathological examination revealed that RAFF inhibited the inflammatory cell infiltration and cell boundary loss caused by CCl4 in the liver. Thus, RAFF is a natural antioxidant with significant antioxidative activities and liver protective effects. The results of this study may be relevant for enhancing the application of the red-fleshed apple fruit extract as a food additive. PRACTICAL APPLICATION: We took the self-selected red-fleshed apple cultivar "Meihong" as the unique research material, and the active ingredients of its flavonoid extract, in vitro antioxidant activity and hepatoprotective effect were analyzed. It is of great significance to promote the development of the red-fleshed apple industry, and also provides an important reference for the development of natural antioxidants.
- Published
- 2020
29. Research on temperature-assisted laser transmission welding of copper foil and polyethylene terephthalate
- Author
-
Yisai Wang, Hao Liu, Le Jia, Baocai Zhang, Jingbin Hao, and Haifeng Yang
- Subjects
0209 industrial biotechnology ,Materials science ,Strategy and Management ,Laser beam welding ,chemistry.chemical_element ,Welding joint ,02 engineering and technology ,Welding ,Management Science and Operations Research ,021001 nanoscience & nanotechnology ,Copper ,Industrial and Manufacturing Engineering ,Flexible electronics ,law.invention ,020901 industrial engineering & automation ,chemistry ,Optical microscope ,law ,Ultimate tensile strength ,Surface roughness ,Composite material ,0210 nano-technology - Abstract
Copper foil is widely used in printed circuit board because of its good conductivity, especially in flexible printed circuit board. A traditional two-layer flexible copper clad laminate avoids introduction of an additional adhesive, its application range is improved, but its peel strength is low. Laser, as an efficient and clean energy, has great potential application value in this field. Based on the research of parameters of laser transmission welding copper foil and polyethylene terephthalate (PET), to prepare a two-layer flexible copper clad plate and improve the bonding strength of welding joints in laser welding, this study proposes to change the surface roughness of the copper foil and preheat the weldment in the welding process. The bonding properties of the welded joint were tested by a micro-tensile test bench. Through the study of laser parameters, a welding speed of 300 mm/min was used to explore the surface roughness of the copper foil, it was found that the overall welding effect was best when the surface roughness of the copper foil was 400 nm. The effect of the preheating temperature on the bonding properties of weldments was studied by preheating samples at different temperatures. A high-speed camera was used to record the instantaneous tensile state of the welding sample, the surface roughness of the debonding sample under different conditions was measured by atomic force microscope. The interface morphology of the sample was characterized by optical microscope, X-ray photoelectron spectrometer, and high-resolution field emission scanning electron microscope. The bonding mechanism was revealed, and the reason for the strength improvement of the welding joint was discussed. In view of the research results of the PET/copper foil high-strength bonding in this paper, it shows that the laser welding technology has an important application prospect in the production of a two-layer flexible copper clad laminate.
- Published
- 2020
30. Mitochondrial Translocation of DJ-1 Is Mediated by Grp75
- Author
-
Xue-Ying Wang, Lei Tang, Jiao Huang, Zhao-Xia Ma, Yi-Zhang Deng, He-Ping Chen, Xing-Wang Xu, Le-Jia Qiu, Hao-Yue Liu, and Ting-Ting Zhou
- Subjects
0301 basic medicine ,Small interfering RNA ,Protein Deglycase DJ-1 ,Myocardial Reperfusion Injury ,Chromosomal translocation ,030204 cardiovascular system & hematology ,Mitochondrion ,medicine.disease_cause ,Antioxidants ,Mitochondria, Heart ,Cell Line ,Electron Transport Complex IV ,Mitochondrial Proteins ,03 medical and health sciences ,NDUFA4 ,0302 clinical medicine ,medicine ,Animals ,HSP70 Heat-Shock Proteins ,Myocytes, Cardiac ,RNA, Small Interfering ,Pharmacology ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,NADH Dehydrogenase ,Cell Hypoxia ,Rats ,Cell biology ,Oxidative Stress ,Protein Transport ,Cytosol ,030104 developmental biology ,chemistry ,Resveratrol ,Chaperone (protein) ,biology.protein ,RNA Interference ,Cardiology and Cardiovascular Medicine ,Oxidative stress - Abstract
Resveratrol (Res) was recently reported to ameliorate hypoxia/reoxygenation (H/R)-caused oxidative stress in H9c2 cardiomyocytes through promoting the mitochondrial translocation of DJ-1 protein and subsequently preserving the activity of mitochondrial complex I. However, it is noteworthy that DJ-1 possesses no mitochondria-targeting sequence. Therefore, how Res induces DJ-1 mitochondrial translocation is an important and interesting question for further exploration. Glucose-regulated protein 75 (Grp75), whose N-terminus contains a 51-amino acid long mitochondrial-targeting signal peptide, is a cytoprotective chaperone that partakes in mitochondrial import of several proteins. Here, the contribution of Grp75 to mitochondrial import of DJ-1 by Res was investigated in a cellular model of H/R. Our results showed that Res upregulated the expression of DJ-1 protein, enhanced the interaction of DJ-1 and Grp75, and promoted DJ-1 translocation to mitochondria from cytosol in H9c2 cardiomyocytes undergoing H/R. Importantly, knockdown of Grp75 markedly reduced the interaction of DJ-1 with Grp75 and subsequent DJ-1 mitochondrial translocation induced by Res. Furthermore, Res pretreatment promoted the association of DJ-1 with ND1 and NDUFA4 subunits of complex I, preserved the activity of complex I, decreased mitochondria-derived reactive oxygen species production, and eventually ameliorated H/R-caused oxidative stress damage. Intriguingly, these effects were largely prevented also by small interfering RNA targeting Grp75. Overall, these results suggested that Grp75 interacts with DJ-1 to facilitate its translocation from cytosol to mitochondria, which is required for Res-mediated preservation of mitochondria complex I and cardioprotection from H/R-caused oxidative stress injury.
- Published
- 2020
31. Ru‐Catalyzed Switchable N ‐Hydroxyethylation and N ‐Acetonylation with Crude Glycerol
- Author
-
Yuehui Li, Chen-Xia Du, Yuxing Huang, Zhuo Xin, and Le Jia
- Subjects
Chemistry ,General Chemical Engineering ,02 engineering and technology ,Raw material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,General Energy ,Glycerol ,Environmental Chemistry ,Organic chemistry ,General Materials Science ,0210 nano-technology ,Bond cleavage - Abstract
Highly efficient Ru-catalyzed selective C-C or C-O bond cleavage of polyols (e.g., crude glycerol) for N-hydroxyethylation or N-acetonylation of amines was achieved through the hydrogen-borrowing approach. A variety of amines were transformed to the desired amino alcohols/ketones in moderate-to-excellent yields, opening up new avenues for generation of oxygenated pharmaceuticals and fine chemicals from renewable raw materials. The use of new redox-active catalysts containing bisphosphine/thienylmethylamine ligands allows this hydrogen-borrowing system to be operated selectively under both basic and acidic conditions.
- Published
- 2020
32. BiOBr/Ag6Si2O7 heterojunctions for enhancing visible light catalytic degradation performances with a sequential selectivity enabled by dual synergistic effects
- Author
-
Kun-Le Jia, Xiao-Jie Yu, Ya-Qiong Jing, Shu-Meng Hao, Jin Qu, Zhong-Shuai Zhu, Zhong-Zhen Yu, and Yasmine Abdelkrim
- Subjects
Aqueous solution ,Chemistry ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Biomaterials ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Adsorption ,Reaction rate constant ,Photocatalysis ,Methyl orange ,0210 nano-technology ,Selectivity ,Photodegradation ,Visible spectrum - Abstract
Efficient separation of photogenerated electron-hole pairs is always one of the key factors boosting visible light photodegradation efficiency. Till now, there are few reports on the synergistic competitive consumption of photogenerated active species and the synergistic adsorption of organic contaminants to promote the performance of a designed heterojunction. Herein, we design and construct a novel BiOBr/Ag6Si2O7 heterojunction with the dual synergistic effects towards methylene blue (MB) and methyl orange (MO). The dual synergistic effects could avoid the combination of photogenerated h+/e− pairs, improve the adsorption efficiency, and even regulate the photodegradation efficiency. Thus, for an aqueous mixture of MB and MO, the BiOBr/Ag6Si2O7 photocatalyst exhibits largely improved adsorption capacities of the dyes by a multi-layer adsorption mode. Moreover, the photocatalyst could further promote the photodegradation rate of MO while slow that of MB due to the competitive consumption of photogenerated active species, showing a sequential selectivity phenomenon. Thanks to the dual synergistic effects, the adsorption capacity of MO increases 1379% higher than that of neat MO solution, and the photodegradation time decrease from 30 to 12 min with a rate constant of 0.22 min−1, 38% higher than that of neat MO solution.
- Published
- 2020
33. Glucopyranose from
- Author
-
Xinling, Song, Weijun, Cui, Fanyun, Meng, Qun, Xia, Xueping, Li, Minjie, Hou, Le, Jia, and Jianjun, Zhang
- Subjects
Male ,Toll-Like Receptor 4 ,Disease Models, Animal ,Mice ,Glucose ,NF-E2-Related Factor 2 ,Polysaccharides ,Animals, Outbred Strains ,NF-kappa B ,Animals ,Pleurotus ,Liver Diseases, Alcoholic ,Gastrointestinal Microbiome - Abstract
This study investigated the effects of PGPs (
- Published
- 2022
34. Fast In-Situ Triaxial Remanent Magnetic Field Measurement for Single-Beam Serf Atomic Magnetometer Based on Trisection Algorithm
- Author
-
Tengyue Long, Bangcheng Han, Xinda Song, Yuchen Suo, Le Jia, and Wen Ren
- Published
- 2022
35. Can fractal dimensions objectivize gastropod shell morphometrics? A case study from Lake Lugu (SW China)
- Author
-
Wiese, Robert, Harrington, Kyle, Hartmann, Kai, Hethke, Manja, von Rintelen, Thomas, Zhang, Hucai, Zhang, Le-Jia, and Riedel, Frank
- Subjects
landmark analysis ,500 Naturwissenschaften und Mathematik::560 Fossilien, Pal��ontologie::560 Pal��ontologie, Pal��ozoologie ,morphometrics ,Ancient lake ,3D models ,3D fractal dimensions ,non-marine gastropods ,Viviparidae ,DNA sequencing ,Technology Platforms - Abstract
1. Morphometrics are fundamental for the analysis of size and shape in fossils, particularly because soft parts or DNA are rarely preserved and hard parts such as shells are commonly the only source of information. Geometric morphometrics, that is, landmark analysis, is well established for the description of shape but it exhibits a couple of shortcomings resulting from subjective choices during landmarking (number and position of landmarks) and from difficulties in resolving shape at the level of micro-sculpture. 2. With the aid of high-resolution 3D scanning technology and analyses of fractal dimensions, we test whether such shortcomings of linear and landmark morphometrics can be overcome. As a model group, we selected a clade of modern viviparid gastropods from Lake Lugu, with shells that show a high degree of sculptural variation. Linear and landmark analyses were applied to the same shells in order to establish the fractal dimensions. The genetic diversity of the gastropod clade was assessed. 3. The genetic results suggest that the gastropod clade represents a single species. The results of all morphometric methods applied are in line with the genetic results, which is that no specific morphotype could be delimited. Apart from this overall agreement, landmark and fractal dimension analyses do not correspond to each other but represent data sets with different information. Generally, the fractal dimension values quantify the roughness of the shell surface, the resolution of the 3D scans determining the level. In our approach, we captured the micro-sculpture but not the first-order sculptural elements, which explains that fractal dimension and landmark data are not in phase. 4. We can show that analyzing fractal dimensions of gastropod shells opens a window to more detailed information that can be considered in evolutionary and ecological contexts. We propose that using low-resolution 3D scans may successfully substitute landmark analyses because it overcomes the subjective landmarking. Analyses of 3D scans with higher resolution than used in this study will provide surface roughness information at the mineralogical level. We suggest that fractal dimension analyses of a combination of differently resolved 3D models will significantly improve the quality of shell morphometrics.
- Published
- 2022
- Full Text
- View/download PDF
36. A Fast Determination Method for Transverse Relaxation Rate of Minimized SERF Atomic Magnetometer
- Author
-
Le Jia, Xinda Song, Jianli Li, Yuchen Suo, Chaoxin Yang, Tengyue Long, and Xiaolin Ning
- Subjects
Electrical and Electronic Engineering ,Instrumentation - Published
- 2023
37. Efficacy of Pleurotus djamor Zinc Polysaccharide Chewable Tablets against Reproductive Damage Induced by Nonylphenol
- Author
-
Mengyu Zhang, Wenshuai Wang, Wenxue Sun, Jianjun Zhang, and Le Jia
- Subjects
Organic Chemistry ,Food Science - Published
- 2022
38. Asymmetric molten pool morphology in wire-arc directed energy deposition: Evolution mechanism and suppression strategy
- Author
-
Huajun Cao, Rufeng Huang, Hao Yi, MengLin Liu, and Le Jia
- Subjects
Biomedical Engineering ,General Materials Science ,Engineering (miscellaneous) ,Industrial and Manufacturing Engineering - Published
- 2022
39. A self-testing kits distribution intervention improved HIV testing of men who have sex with men and their sexual partners: a 12-month stepped-wedge randomized controlled trial in China (Preprint)
- Author
-
Xia Jin, Zhenxing Chu, Xiangjun Zhang, Tianyi Lu, Jing Zhang, Hongyi Wang, Zehao Ye, Sitong Cui, Zhaozhen Liu, Jie Wei, Le Jia, Yangyang Gao, Qinghai Hu, Yongjun Jiang, Ning Wang, Hong Shang, and Junjie Xu
- Abstract
BACKGROUND Men who have sex with men (MSM) have high HIV incidence and prevalence burdens but relatively low HIV testing rates. Literature showed insufficient evidence on the efficacy of Social media (WeChat) based HIV self-testing (HIVST) kits distribution approaches among MSM and their sexual partners. OBJECTIVE This study evaluated an Social media (WeChat) based HIVST distribution intervention in increasing HIV testing uptake among MSM and their sexual partners in China. METHODS The study used a 12-month stepped-wedge randomized controlled trial design and an online survey mode. MSM who were HIV-negative or with unknown HIV status were recruited through social media marketing and outreaches by community opinion leaders between August and December 2018 in Shenyang, Beijing, Chongqing, and Shenzhen. Participants were randomly allocated to four groups, which were intervened by sequence. The intervention group received free HIVST kits on top of the control condition that participants received standard education and care. Participants were followed up from January to December 2019. Generalized linear models were used to assess HIV testing coverage and frequency difference between the intervention and the control stage. RESULTS Each group enrolled 140 eligible MSM with a total of 560 participants. Twelve participants were diagnosed with HIV infections, and 4 of them were in the follow-up period. Participants in the intervention stage were nine times likely to receive an HIV test than in the control stage (85.6% vs. 39.2%, risk ratio [RR]=9.21, 95%CI 5.92~14.33). The intervention also increased participants’ HIV testing frequency (1.48 vs. 0.59 times, risk difference [RD]=0.89, 95%CI 0.82~0.96). Moreover, the intervention increased HIV testing proportion (49.0% vs. 30.1%, RR=2.23, 95%CI 1.46~3.40) and mean HIV testing frequency (0.69 vs. 0.35 times, RD=0.26, 95%CI 0.15~0.38) among participants sexual partners who received HIVST through secondary distribution. CONCLUSIONS An Social media (WeChat) based HIVST distribution intervention effectively increased HIV testing coverage and frequency in MSM and their sexual partners. Future programs could apply this approach in HIV education and testing efforts to reduce HIV incidence. CLINICALTRIAL This study was registered at the Chinese Clinical Trials website (http://www.chictr.org.cn/index.aspx) with the registration tracking number of ChiCTR1800019453 on November 12, 2018. INTERNATIONAL REGISTERED REPORT RR2-10.2196/17788
- Published
- 2021
40. Can fractal dimensions objectivize gastropod shell morphometrics? A case study from Lake Lugu (SW China)
- Author
-
Robert, Wiese, Kyle, Harrington, Kai, Hartmann, Manja, Hethke, Thomas, von Rintelen, Hucai, Zhang, Le-Jia, Zhang, and Frank, Riedel
- Abstract
Morphometrics are fundamental for the analysis of size and shape in fossils, particularly because soft parts or DNA are rarely preserved and hard parts such as shells are commonly the only source of information. Geometric morphometrics, that is, landmark analysis, is well established for the description of shape but it exhibits a couple of shortcomings resulting from subjective choices during landmarking (number and position of landmarks) and from difficulties in resolving shape at the level of micro-sculpture.With the aid of high-resolution 3D scanning technology and analyses of fractal dimensions, we test whether such shortcomings of linear and landmark morphometrics can be overcome. As a model group, we selected a clade of modern viviparid gastropods from Lake Lugu, with shells that show a high degree of sculptural variation. Linear and landmark analyses were applied to the same shells in order to establish the fractal dimensions. The genetic diversity of the gastropod clade was assessed.The genetic results suggest that the gastropod clade represents a single species. The results of all morphometric methods applied are in line with the genetic results, which is that no specific morphotype could be delimited. Apart from this overall agreement, landmark and fractal dimension analyses do not correspond to each other but represent data sets with different information. Generally, the fractal dimension values quantify the roughness of the shell surface, the resolution of the 3D scans determining the level. In our approach, we captured the micro-sculpture but not the first-order sculptural elements, which explains that fractal dimension and landmark data are not in phase.We can show that analyzing fractal dimensions of gastropod shells opens a window to more detailed information that can be considered in evolutionary and ecological contexts. We propose that using low-resolution 3D scans may successfully substitute landmark analyses because it overcomes the subjective landmarking. Analyses of 3D scans with higher resolution than used in this study will provide surface roughness information at the mineralogical level. We suggest that fractal dimension analyses of a combination of differently resolved 3D models will significantly improve the quality of shell morphometrics.
- Published
- 2021
41. Liver and Brain Protective Effect of Sulfated Polysaccharides from Residue of Lion's Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes), on D-Galactose-Induced Aging Mice
- Author
-
Wenbo Liu, Yanbo Feng, Fangfang Yuan, Huaping Li, Huajie Zhao, Le Jia, and Yiwen Zhang
- Subjects
Aging ,Antioxidant ,Hericium ,medicine.medical_treatment ,Polysaccharide ,Applied Microbiology and Biotechnology ,chemistry.chemical_compound ,Mice ,Sulfation ,Functional food ,In vivo ,Polysaccharides ,Drug Discovery ,medicine ,Animals ,Pharmacology ,chemistry.chemical_classification ,biology ,Chemistry ,Sulfates ,Brain ,Galactose ,biology.organism_classification ,Enzyme ,Biochemistry ,Liver ,Hericium erinaceus - Abstract
This work describes the preparation of sulfated Hericium erinaceus residue polysaccharides (SHRPs) and investigates their antioxidant and antiaging effects on D-galactose-induced aging mice. The results indicated that the degree of SO42- substitution was 0.47 ± 0.22 for SHRPs. In vitro analysis and in vivo animal experiments manifested that SHRPs could alleviate aging properties by scavenging radicals, elevating enzyme activities, and reducing malondialde-hyde content, thus improving serum biochemical indices and enhancing immunological activity. Liver and brain injuries of mice could be remitted by SHRP interventions. Structural characteristics of SHRPs in this work were elucidated by Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry, and the results suggest that SHRPs could be used as an effective dietary supplement and functional food for prevention of aging and age-related diseases.
- Published
- 2021
42. Lentinus edodes Polysaccharides Alleviate Acute Lung Injury by Inhibiting Oxidative Stress and Inflammation
- Author
-
Yiwen Zhang, Yanfei Cui, Yanbo Feng, Fengping Jiao, and Le Jia
- Subjects
Chemistry (miscellaneous) ,antioxidant activities ,anti-inflammatory ,acute lung injury ,Lentinula edodes polysaccharides ,pulmonary protection ,Organic Chemistry ,Drug Discovery ,Molecular Medicine ,Pharmaceutical Science ,Physical and Theoretical Chemistry ,Analytical Chemistry - Abstract
Acute lung injury (ALI) is a kind of lung disease with acute dyspnea, pulmonary inflammation, respiratory distress, and non-cardiogenic pulmonary edema, accompanied by the mid- and end-stage characteristics of COVID-19, clinically. It is imperative to find non-toxic natural substances on preventing ALI and its complications. The animal experiments demonstrated that Lentinus edodes polysaccharides (PLE) had a potential role in alleviating ALI by inhibiting oxidative stress and inflammation, which was manifested by reducing the levels of serum lung injury indicators (C3, hs-CRP, and GGT), reducing the levels of inflammatory factors (TNF-α, IL-1β, and IL-6), and increasing the activities of antioxidant enzymes (SOD and CAT) in the lung. Furthermore, PLE had the typical characteristics of pyran-type linked by β-type glycosidic linkages. The conclusions indicated that PLE could be used as functional foods and natural drugs in preventing ALI.
- Published
- 2022
43. Enzymatic-extractable polysaccharides from Cordyceps militaris alleviate carbon tetrachloride-induced liver injury via Nrf2/ROS/NF-κB signaling pathway
- Author
-
Huajie Zhao, Baoguo Deng, Duan Li, Le Jia, and Fan Yang
- Subjects
Nutrition and Dietetics ,Medicine (miscellaneous) ,Food Science - Published
- 2022
44. A new sinistral species of the land-snail genus Satsuma (Pulmonata: Camaenidae) from China
- Author
-
Zhi-Tong Lyu, Le-Jia Zhang, and Yi-Jie Zhu
- Subjects
0106 biological sciences ,Camaenidae ,biology ,Ecology ,010604 marine biology & hydrobiology ,Biogeography ,Land snail ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Pulmonata ,Sinistral and dextral ,Genetics ,Animal Science and Zoology ,East Asia ,Taxonomy (biology) ,China ,Ecology, Evolution, Behavior and Systematics - Abstract
The species of Satsuma are mostly endemic to East Asia except for one species distributed in Batan Island of the Philippines. More than 99% of the known species of this genus are endemic to the isl...
- Published
- 2019
45. The ameliorations of Ganoderma applanatum residue polysaccharides against CCl4 induced liver injury
- Author
-
Chen Zhang, Huaping Li, Jianjun Zhang, Yiwen Zhang, Fangfang Yuan, Yanbo Feng, Le Jia, Zheng Gao, and Zhen Song
- Subjects
Antioxidant ,medicine.drug_class ,medicine.medical_treatment ,CCL4 ,02 engineering and technology ,Pharmacology ,Polysaccharide ,Biochemistry ,Anti-inflammatory ,03 medical and health sciences ,Ganoderma applanatum ,Structural Biology ,medicine ,Molecular Biology ,030304 developmental biology ,Liver injury ,chemistry.chemical_classification ,0303 health sciences ,biology ,General Medicine ,CYP2E1 ,021001 nanoscience & nanotechnology ,medicine.disease ,biology.organism_classification ,IκBα ,chemistry ,0210 nano-technology ,hormones, hormone substitutes, and hormone antagonists - Abstract
This work investigated the protective effects of Ganoderma applanatum residue polysaccharides (GRP) on the CCl4-induced hepatotoxicity. The results indicated that GRP showed significantly effects on preventing the increase of AST, ALT and ALP levels in serum, elevating the activities of SOD, GSH-Px and CAT, decreasing the contents of MDA and LPO, and reducing the CYP2E1 and TGF-β concentrations in CCl4-induced mice, respectively. Meanwhile, the levels of TNF-α and IL-6 were significantly decreased, while the value of IL-10 was increased by GRP treatment. Besides, the western blot assay showed the IκBα expressions were significantly increased and the p-p65 was decreased by the treatment with GRP. The characterizations indicated that the GRP was heteropolysaccharide with lower molecular weights and α-furanoside residues. These results demonstrated that GRP might be a potential material for drug and functional food development against chemical hepatic injury.
- Published
- 2019
46. Antioxidant and hepatoprotective activities of residue polysaccharides by Pleurotus citrinipileatus
- Author
-
Jianjun Zhang, Huajie Zhao, Le Jia, Xinchao Liu, Hui Pang, and Zheng Gao
- Subjects
Antioxidant ,medicine.medical_treatment ,02 engineering and technology ,Pharmacology ,Pleurotus ,Protective Agents ,Polysaccharide ,Biochemistry ,Antioxidants ,Mice ,03 medical and health sciences ,Structural Biology ,medicine ,Animals ,Molecular Biology ,Chromatography, High Pressure Liquid ,030304 developmental biology ,chemistry.chemical_classification ,Liver injury ,0303 health sciences ,biology ,Spectrum Analysis ,Monosaccharides ,Fungal Polysaccharides ,Glycosidic bond ,General Medicine ,CYP2E1 ,021001 nanoscience & nanotechnology ,medicine.disease ,biology.organism_classification ,Cytokine ,Liver ,chemistry ,Hepatocytes ,0210 nano-technology ,Biomarkers ,TBIL - Abstract
The enzymatic-extraction residue polysaccharide (ERPS) from Pleurotus citrinipileatus was obtained, and its antioxidant and hepatoprotective effects were also investigated. Animal experiments indicated that the ERPS could reduce the levels of AST, ALT, ALP, TBIL, MDA and LPO, improve the activities of GSH-Px, SOD and CAT, decrease the inflammatory factor of CYP2E1, TNF-α and IL-6, and enhance the IL-10 levels, showing the potential protections against CCl4-induced injures. ERPS can improve liver fibrosis by reducing the level of pivotal cytokine TGF-β1. Western blotting results revealed that ERPS relieved the inflammatory response by increasing the I-κBα expressions in the NF-κB pathway. Furthermore, the structural characteristics demonstrated that the ERPS was a typical β-type glycosidic pyranose with the weight-average molecular weight of 1.30 × 105 Da and the monosaccharide composition of Man, Rha, Glc, Gal, Xyl and Ara, GlcUA and GalUA. These results demonstrated that the ERPS might be suitable for functional foods and natural drugs for preventing the acute liver damage.
- Published
- 2019
47. Characterization and anti-hyperlipidemia effects of enzymatic residue polysaccharides from Pleurotus ostreatus
- Author
-
Yuhan Dong, Jianjun Zhang, Xiuxiu Wang, Le Jia, Zheng Gao, Huajie Zhao, and Guiyu Sun
- Subjects
Male ,Antioxidant ,medicine.medical_treatment ,02 engineering and technology ,Pleurotus ,Polysaccharide ,medicine.disease_cause ,Biochemistry ,Antioxidants ,Mice ,03 medical and health sciences ,Residue (chemistry) ,Structural Biology ,Hyperlipidemia ,Toxicity Tests, Acute ,medicine ,Animals ,Food science ,Molecular Biology ,Hypolipidemic Agents ,030304 developmental biology ,Liver injury ,chemistry.chemical_classification ,0303 health sciences ,biology ,Spectrum Analysis ,Fungal Polysaccharides ,General Medicine ,Glucose Tolerance Test ,Lipid Metabolism ,021001 nanoscience & nanotechnology ,medicine.disease ,biology.organism_classification ,Lipids ,Oxidative Stress ,Enzyme ,Liver ,chemistry ,Pleurotus ostreatus ,0210 nano-technology ,Biomarkers ,Oxidative stress - Abstract
The aims of this work were to extract the enzymatic residue polysaccharides (EnRPS) from Pleurotus ostreatus and investigate the antioxidant and anti-hyperlipidemic effects on high-fat-high-cholesterol emulsion (HFHCE)-induced liver injured mice. The results revealed that EnRPS at 400 mg/kg bw showed superior liver protective effects by ameliorating the AI, lowing the hepatic MPO, FFA, ADPN, TC and TG indicators, and improving the antioxidant status by enhancing liver enzyme activities (SOD, CAT, LEP, INS and T-AOC), eliminating the MDA content, decreasing the levels of LDL-C, TC, TG, ALT, AST and ALP, and increasing the HDL-C in serum. And in addition, GC, FTIR, NMR and pathological sections were also studied. The above consequences suggested that EnRPS could be used as functional foods of oxidative stress and anti-hyperlipidemic against liver injury.
- Published
- 2019
48. Anti-hyperlipidemic, antioxidant and organic protection effects of acidic-extractable polysaccharides from Dictyophora indusiata
- Author
-
Xinling Song, Xiuxiu Wang, Le Jia, Wenshuai Wang, Min Liu, Zheng Gao, and Huajie Zhao
- Subjects
Male ,Antioxidant ,medicine.medical_treatment ,02 engineering and technology ,Protective Agents ,Polysaccharide ,medicine.disease_cause ,Biochemistry ,Antioxidants ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Functional food ,Structural Biology ,Hyperlipidemia ,medicine ,Animals ,Food science ,Molecular Biology ,Hypolipidemic Agents ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Lipid peroxide ,Chemistry ,Basidiomycota ,Spectrum Analysis ,Fungal Polysaccharides ,General Medicine ,Lipid Metabolism ,021001 nanoscience & nanotechnology ,medicine.disease ,Molecular Weight ,Liver ,Urea ,0210 nano-technology ,Biomarkers ,TBIL ,Oxidative stress - Abstract
In the present work, we investigated the anti-hyperlipidemic, antioxidant and organic protection effects of acidic-extractable Dictyophora indusiata polysaccharides (Ac-DPS) on hyperlipidemic mice induced by high-fat emulsion. The results demonstrated that Ac-DPS had impressive abilities to mitigate oxidative stress by increasing the activities of antioxidant enzyme and reducing the contents of lipid peroxide. Moreover, lipid levels in serum were returned to normal status. Besides, Ac-DPS exhibited potential hepatic and renal protection effects reflected by decreasing serum enzyme activities, lowering TBIL, UREA and CREA levels and increasing ALB content. At the same time, histopathological observations proved protective effects of Ac-DPS on organs. Subsequently, the physical properties of polysaccharide were also investigated by HPGPC and FT-IR. The above consequences confirmed the important role of Ac-DPS as a functional food and natural medicine in the fight against oxidative stress and the prevention of hyperlipidemia.
- Published
- 2019
49. Response and intraspecific differences in nitrogen metabolism of alfalfa (Medicago sativa L.) under cadmium stress
- Author
-
Le Jia, Shu Yang, Yufen Bi, Yanqun Zu, Yuan Li, Bo Li, and Yongmei He
- Subjects
Environmental Engineering ,Proline ,Nitrogen ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,chemistry.chemical_element ,02 engineering and technology ,010501 environmental sciences ,Nitrate reductase ,Nitrate Reductase ,Plant Roots ,01 natural sciences ,Soil ,chemistry.chemical_compound ,Animal science ,Species Specificity ,Glutamate-Ammonia Ligase ,Glutamate synthase ,Glutamine synthetase ,Ammonium Compounds ,Environmental Chemistry ,Ammonium ,Biomass ,Amino Acids ,0105 earth and related environmental sciences ,Cadmium ,biology ,Glutamate dehydrogenase ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Pollution ,020801 environmental engineering ,chemistry ,Shoot ,biology.protein ,Medicago sativa - Abstract
Pot experiments were carried out to evaluate the response and intraspecific differences in nitrogen metabolism of 20 alfalfa cultivars under cadmium stress. To the aim, exogenous cadmium was added into soil with concentration of 0 (control) and 50 mg kg−1. Results showed that 20 alfalfa were ranked as following according to response index: Guochan (550.93) > Deqin (372.50) > Caoyuan No.1 (350.26) > Queen (345.45) > Xinmu No.2 (344.43) > Longzhong (274.85) > Victoria (233.13) > Emperor (233.13) > Giant (192.29) > Qianjing (101.21) > Xinjiangdaye (75.72) > Algonuin (−32.55) > Duoye (−62.44) > Altay (−102.77) > Sandeli (−155.02) > Turist (−193.24) > Gannong No.1 (−199.22) > Sijiwang (−245.14) > Zhongmu No.1 (−245.48) > WL525HQ (−268.26). Guochan was identified as cadmium tolerant cultivar. Compared with the control group, its plant height increased by 40.96%, shoot and root biomass respectively increased by 18.10% and 70.19%, total nitrogen content in shoot and root respectively increased by 26.69% and decreased by 12.59%, nitrate content decreased by 7.05%, content of ammonium, proline, free amino acid and soluble protein respectively increased by 13.67%, 89.63%, 28.09% and 14.86%, activity of nitrate reductase, glutamine synthetase, glutamate synthase and glutamate dehydrogenase increased respectively 58.52%, 36.63%, 97.79% and 75.44%. WL525HQ, its above indicators appeared significant differences with those of Guochan, was identified as cadmium sensitive cultivar. In conclusion that the nitrogen metabolism process played an important role for alfalfa to adapt cadmium stress, and the response of nitrogen metabolism to cadmium stress varied with different alfalfa cultivars.
- Published
- 2019
50. Characteristic anti-inflammatory and antioxidative effects of enzymatic- and acidic- hydrolysed mycelium polysaccharides by Oudemansiella radicata on LPS-induced lung injury
- Author
-
Chen Zhang, Zhenzhen Ren, Xinchao Liu, Fulan Zhao, Nuo Xu, Le Jia, Wenshuai Wang, Yuhan Dong, Qihang Yang, Xinling Song, Jianjun Zhang, and Zheng Gao
- Subjects
Male ,congenital, hereditary, and neonatal diseases and abnormalities ,Antioxidant ,Polymers and Plastics ,medicine.drug_class ,medicine.medical_treatment ,02 engineering and technology ,Pharmacology ,Lung injury ,Protective Agents ,010402 general chemistry ,Polysaccharide ,01 natural sciences ,Antioxidants ,Anti-inflammatory ,Mice ,Hydrolysis ,Cellulase ,Multienzyme Complexes ,Polysaccharides ,Interleukin-1alpha ,Materials Chemistry ,medicine ,Animals ,skin and connective tissue diseases ,Lung ,Mycelium ,Glucuronidase ,chemistry.chemical_classification ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Anti-Inflammatory Agents, Non-Steroidal ,Organic Chemistry ,nutritional and metabolic diseases ,Lung Injury ,021001 nanoscience & nanotechnology ,In vitro ,0104 chemical sciences ,Polygalacturonase ,Enzyme ,chemistry ,Agaricales ,0210 nano-technology - Abstract
The present work investigated the antioxidative, anti-inflammatory and pulmonary protective effects of enzymatic- and acid- hydrolysed mycelia polysaccharides (En-MPS and Ac-MPS) from Oudemansiella radicata on LPS-induced acute lung injury (ALI) mice. The results demonstrated that both En-MPS and Ac-MPS showed potential pulmonary protective effects by decreasing serum levels of hs-CRP and C3, increasing pulmonary enzyme values of SOD, GSH-Px, CAT and the level of T-AOC; reducing the activity of MPO; and down-regulating the contents of MDA and LPO. In addition, the levels of TNF-ɑ, IL-1β, and IL-6 in BALF of mice treated with En-MPS at a dosage of 400 mg/kg/d were significantly lower than those in the ALI mice. The in vitro antioxidant effects also showed that the En-MPS was more effective than Ac-MPS. Furthermore, the physical properties of polysaccharides were also investigated by GC, HPGPC, FT-IR and NMR. These results indicated that both En-MPS and Ac-MPS possessed potent antioxidant and anti-inflammatory activities, which could be used as an ingestible drug in preventing lung injury.
- Published
- 2019
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.