Search

Objective The objective of this study was to compare the quality of dry-aged beef from cull Hanwoo cows slaughtered at 60 or 80 months old. Methods A total of eight cull Hanwoo carcasses with a quality grade of 3 (low-grade) were selected and divided into two age groups: 63.5±2.5 months old (n = 4) and 87.8±4.5 months old (n = 4). Whole longissimus thoracis et lumborum from the 11th rib to the last lumbar vertebrae, including the back fat, was removed from the carcass at 24 h postmortem and aged for 50 days in darkness at a temperature of 2°C±1°C, a relative humidity of 85% and an air flow of 2 m/s. The sampling was performed aseptically after 0, 20, 24, 40, and 50 days of aging. Results Regardless of the aging period, aging increased the lightness (p

Additional file 1: Fig S1. Schematic representation of synthesized PEG-CZNPs and FITC conjugated PEG-CZNPs. A The process comprises two steps; (i) preparation of CZNPs based on non-hydrolytic sol-gel reaction in oleylamine; (ii) transfer of the as-synthesized CZNPs to aqueous phase by modifying with phospholipid-PEG. B FITC-conjugated phospholipid-PEG capped CZNPs were prepared with a thiourea linkage. C Analysis of Fourier transform infrared spectroscopy (FT-IR) spectra comparing the as synthesized PEG-CZNP and FITC conjugated PEG-CZNP with FITC. (a) PEG-CZNP, (b) FITC and (c) FITC conjugated PEG-CZNP. Fig. S2. Expression of key differentiation markers in human podocytes. A, B Representative confocal immunofluorescence microscopy images of podocin, nephrin and synaptopodin (green), with DAPI counterstaining (blue), in both undifferentiated and differentiated human podocytes. Fig. S3. Validation of ��-GLA knockdown efficiency by quantitative real-time RT-PCR analysis and immunoblotting. A, B Quantitative real-time RT-PCR and immunoblotting results showing the ��-GLA knockdown efficiency in HK-2 cells and human podocytes. Data values represent the mean �� SD; *P

Additional file 2: Figure S1. Comparison of the present study and two previous CVF proteomic studies from pregnant women. Figure S2. Venn diagram illustrating the overlap of protein identifications between the present study and three previous CVF proteomic studies from non-pregnant women.

The purpose of this study is to comparatively analyze the effects of ankle strength exercise and functional electrical stimulation on ability balance control and gait of stroke patients. The results of the study will be used as basic data for help in independent life of stroke patients. Subject person of this study is 20 stroke patients, sex ratio was 10 men, 10 women. Experimental group (5 men, 5 woman) ran parallel ankle strength exercise and functional electric stimulation treatment, and control group (5 men, 5 woman) ran parallel functional electric stimulation treatment and exercise treatment. Measured balance ability and walking speed, ankle angle after 8 weeks experiment 3 inning a week. The results were as follows; 1. Weight bearing rate by Berg balance scale and BPM increased to keep in mind in experimental grooup and control group, and change that position sway by BPM, sway path, sway area, the all sway speeds displayed decrease that keep in mind, and are less relatively in control group was seen. And difference of change amount between two groups displayed statistically significant. It means that this improved two groups total balance ability but ankle strength exercise and same time of functional electric stimulation treatment are more effect. 2. Change of walking speed and ankle joint angle displayed decrease that two groups keep in mind after all experiments in TUG that display walking speed in experimental group and control group. But, ankle joint angle both was no difference that two groups keep in mind after and experiment. And difference that keep in mind in walking speed too as result that significant difference of change amount of two groups appeared. When applied running parallel ankle strength exercise and functional electric stimulation treatment in stroke patients, both experimental group and control group appeared that balance and ability to walk are increased. Also, was expose that difference that keep in mind between group as result that significant difference of change amount of two groups is. Can explain that this means that ankle strength exercise and same time of functional electric stimulation treatment were more effective in treatment of stroke patient.