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Supplemental Material, sj-pdf-1-asu-10.1177_00031348221101492 for Complications and Transfusions on Therapeutic Anticoagulation After Trauma by Anaar E. Siletz, Navpreet K. Dhillon, Nicole M. Fierro, Tobias Muñiz, Priya Loran, Matthew Singer, Yassar M. Hashim, and Eric J. Ley in The American Surgeon

Additional file 1: Table S1. Antibodies used for immunohistochemistry. Fig. S1. No evidence of hypoxic ischemic injury in our VILI model demonstrated by the lack of hypoxic ischemic factor-1α (HIF-1 α) staining in the amygdala compared to an acute ischemic stroke positive control model. Fig. S2. Correlations between lung inflammation, amygdalar cytokine concentrations, and CC3. A–B include SB and VILI animals only while C–D include animals from SB, VILI+Saline, VILI+α-IL-6, and VILI+α-IL-6R. A No significant correlations between %PMNs in BALF and amygdalar IL-1β or between amygdalar IL-1β and amygdalar CC3. B No significant correlation between %PMNs in BALF and hippocampal IL-1β. Direct and significant correlation between hippocampal IL-1β and hippocampal CC3. C No significant correlations between %PMNs in BALF and amygdalar IL-1β or between amygdalar IL-1β and amygdalar CC3. D No significant correlations between %PMNs in BALF and hippocampal IL-1β or between hippocampal IL-1β and hippocampal CC3. Fig. S3. A partial response to the administration α-IL-6 immediately after the completion of ventilation. Independent sample t tests revealed A no significant difference in amygdalar cleaved caspase-3 (CC3) between saline and α-IL-6 treated males. B However, in the hippocampus there was a trend toward significance, with α-IL-6 treated animals have lower CC3. These results indicate early treatment is necessary for the observed effect in both brain regions. N = 5–6 per group and data are expressed in mean ± SD. *p