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Additional file 1: Figure S1. Establishment of YAP1 knockout LNCaP cells. (A) Validation of YAP1 knockout LNCaP cells by CRISPR/Cas9 system. YAP1 expression was determined by immunoblot analysis. All of the results were confirmed by multiple experiments. (B) Validation for CRISPR/Cas9 editing. Representative gel images of T7E1-treated PCR products amplified from the target sites of YAP1. Cleaved-products are designated by arrowheads.

Objectives: This study compared the impact of implant surface treatment on the stability and osseointegration of implants in dog mandibles. Materials and Methods: Six adult dogs received a total of 48 implants that were prepared using four different surface treatments; resorbable blast media (RBM), hydroxyapatite (HA), hydrothermal-treated HA, and sand blasting and acid etching (SLA). Implants were installed, and dogs were separated into 2- and 4-week groups. Implant stability was evaluated via Periotest M, Osstell Mentor, and removal torque analyzers. A histomorphometric analysis was also performed. Results: The stability evaluation showed that all groups generally had satisfactory values. The histomorphometric evaluation via a light microscope revealed that the HA surface implant group had the highest ratio of new bone formation on the entire fixture. The hydrothermal-treated HA surface implant group showed a high ratio of bone-to-implant contact in the upper half of the implant area. Conclusion: The hydrothermal-treated HA implant improved the bone-to-implant contact ratio on the upper fixture, which increased the implant stability.

The purpose of this study is to evaluate the degree of osseointegration of resorbable blasting media (RBM) surface implants retrieved from humans. Three implants in the mandibular molar region that were surface-treated with RBM were retrieved from two patients. The implants were used to manufacture specimens in order to measure the bone-implant contact (BIC) ratio. The BIC ratios of the three implants were found to be an average of 69.0%±9.1%. In conclusion, that RBM surface implants are integrated into the host environment with histological significance and the BIC ratio of the RBM surface-treated implant was not significantly different from that of other surface-treated implants.

The purpose of this study is to assess the combination of particulate dentine and plaster as bone substitute material in calvarial bone defects in rats, and to compare it with a bone xenograft (Bio-Oss ® ). Material and Methods: Forty rats were assigned randomly to five groups and each group was further divided into two subgroups, 8 and 16 weeks after implantation. The defect was filled with different graft materials in each group: Group 1, defects were filled with particulate dentine and plaster using a 2:1 ratio; Group 2, defects were filled with particulate dentine; plaster, and Bio-Oss ® using a 2:1:1 ratio; Group 3, defects were filled with plaster and Bio-Oss ® using a 1:1 ratio; Group 4, defects were filled with Bio-Oss ® only; and Group 5, untreated control defects. Histological sections and histomorphometric analysis of defects were obtained at 8 and 16 weeks postoperatively. Results: New bone formation was highest in Group 4, followed by Group 3, than Group 2, Group 1, and finally the control group. Conclusion: The combination of particulate dentine and plaster is an alternative bone substitute, although it is less effective than Bio-Oss ® .