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Additional file 3. Supplementary methods.

Additional file 2: Fig. S1. Differential 4-hydroxy-2-nonenal (HNE)-modified peptide adducts were re-analyzed through PEAKS 7 using previous MS/MS spectra (ProteomeXchange: PXD004546). Acquired MS/MS spectra were obtained through pooled concanavalin (Con) A-captured serum proteins (nine rheumatoid arthritis (RA) and nine healthy control (HC) pooled samples), 1-D SDS-PAGE, in-gel digestion, and nano-LC-MS/MS (A). HNE reacts with amino acid residues of proteins to form HNE-protein adducts by Michael addition and Schiff base adducts, respectively (B). Representative MS/MS spectrum of the 1211-SHTLRTTCWDGKLEYPTCAK-1230 peptide sequence and the modified peptide bearing an HNE modification at the K1230 residue in RA patients (C). A representative MS/MS spectrum of the peptide sequence 78-AVGDKLPECEADDGCPKPPEIAHGYVEH SVR-108 and the modified peptide bearing the HNE modification at the C92 residue in RA patients (D). The MS/MS spectrum 2-TVAAPSVFIFPPSDEQLK-19 and the modified peptide bearing the HNE modification at the A5 residue in RA patients (E, upper panel); 2-TVAAPSVFIFPPSDEQLK-19 and the modified peptide bearing the HNE modification at the A4 residues in RA patients and HCs (E, bottom panel). Representative MS/MS spectrum of 328-TFGSGEADCGLRPLFEKK-345 and the modified peptide bearing the HNE modification at the K344 residue in RA (F). The MS/MS spectrum 284-HRTGDEITYQCRNGFYPATRGNTAK-308 and the modified peptide bearing the HNE modification at the K308 residue in HCs (G). A representative MS/MS spectrum of the peptide sequence 162-ILGGHLDAK-170 and the modified peptide bearing the HNE modification at the A169 residues in HCs (H). Representative MS/MS spectrum of 83-VYACEVTHQGLSSPVTKSFNR-103 and the modified peptide bearing the HNE modification at the Q91 residue in HCs (I). The MS/MS spectrum 328-TFGSGEADCGLRPLFEK-344 and the modified peptide bearing the HNE modification at the C336 and L341 residues in HCs (J).

Additional file 1: Table S1. Demographic and clinical characteristics of individual subjects contributing to serum for healthy controls (HCs), and patients with osteoarthritis (OA) and rheumatoid arthritis (RA).

Additional file 4: Fig. S2. Comparison of receiver operating characteristics (ROC) curves from unselected features and selected features in (A) decision tree, (B) random forest classifier, and (C) support vector machine classifier.

A compact dual-band dielectric resonator antenna (DRA) using a parasitic c-slot fed by a microstrip line is proposed. In this configuration, the DR performs the functions of an effective radiator and the feeding structure of the parasitic c-slot in the ground plane. By optimizing the proposed structure parameters, the structure resonates at two different frequencies. One is from the DRA with the broadside patterns and the other from the c-slot with the dipole-like patterns. In order to determine the performance of varying design parameters on bandwidth and resonance frequency, the parametric study is carried out using simulation software High-Frequency Structure Simulator and experimental results. The measured and simulated results show excellent agreement.