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Additional file 1: Table S1. Primers detection of SVV by qRT-PCR. Table S2. Primers detection of SVV.

Additional file 3: Fig. S2. Detection of SVV in Culicoides samples. 4/10 of the groups were positive, for group 3, 4, 5, 6 (A); the results of separate sample detection showed that sample 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 25, 30 was positive (B). Table S3. Results of SVV were detected in mosquitoes and Culicoides.

To study the effects of chinaberry tree extract on proliferation, apoptosis, migration and cell cycle distribution of human breast cancer cells, the MCF-7 breast cancer cells obtained were divided into blank group, low concentration group, medium concentration group and high concentration group. Phosphate buffered saline solution and sterile physiological saline were added to the blank group for cell culture. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry were used to detect cell proliferation and apoptosis at different time periods. Transwell assay was used to detect cell migration; flow cytometry was used to detect cell cycle distribution, and intercellular cell adhesion molecule-1, matrix metalloproteinase-2, matrix metalloproteinase-9 were detected by Western blotting. The proliferation rate of MCF-7 breast cancer cells in the low, medium and high concentration group was significantly lower than that in the blank group at different time periods, while the apoptosis rate was significantly higher than that in the blank group, and the effect was more obvious with the increase of concentration (p

In this paper, two recombinant plasmids (pVIR-P12AIL18-3C and pVIR-P12A-3C) containing foot and mouth disease virus (FMDV) capsid polypeptide, 3C coding regions of O/NY00 and using/or not swine IL18 as a genetic adjuvant were constructed, and evaluated for their ability to induce humoral and cellular responses in mice and swine. In addition, the ability to protect swine against homologous virus challenge was examined. Mice and swine were given booster vaccination twice and once, respectively, and swine were challenged 10 days after the booster vaccination. Control groups were inoculated with pVAX1 or phosphate-buffered saline (PBS). All animals vaccinated with pVIR-P12AIL18-3C and pVIR-P12A-3C developed specific anti-FMDV ELISA antibody and neutralizing antibody and T lymphocyte proliferation and CTL cytotoxic activity was observed. In addition, we found that pVIR-P12AIL18-3C possessed stronger immunogenicity than pVIR-P12A-3C. The pVIR-P12AIL18-3C and pVIR-P12A-3C provided full protection in 3/4 and 2/4 swine from challenge with FMDV O/NY00, respectively. The results demonstrate the potential viability of a DNA vaccine in the control and prevention of FMDV infections.

As the demands for Classification of the images is getting higher and higher, users want to search the images more fast and precisely, so a kind of fuzzy decision tree algorithm was presented, which was used to set up a images' emotion classification tree based on the image emotion which had a higher precision. This algorithm is based on the uncertainty of Classification using in images' emotion classification. The algorithm set up a path between the images' vision attributes and the images' emotion attributes, finally let the images can be classified by images' emotion attributes.