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Background: Caregiving burden is common among family caregivers (FCs). In Taiwan, no reports have compared caregiving burden according to disease stage, or explored the comprehensive factors of caregiving burden in the FCs of patients with hepatocellular carcinoma (HCC). Aim: The aim of the study was to investigate caregiving burden at different diagnosis stages and its potential predictors in the FCs of patients with hepatocellular carcinoma. Methods: This descriptive, cross-sectional study included 192 FCs. Caregiving burden was measured using the Caregiver Reaction Assessment tool. The predictive factors of caregiving burden in the FCs of patients with HCC were identified using a linear regression model. Results: The global caregiving burden had no significant differences between the four disease stages. The lack of family support and impact on schedule were significantly higher at the terminal stage than at the earlier stage. The risk factors of caregiving burden were high depression, high financial demand, heavy caregiving tasks, advanced age and frequent patient contact, which obtained a variance of 47.8% in the regression model. Conclusion: Healthcare providers need to proactively identify and assess FCs with risk factors of caregiving burden and provide appropriate interventions specific to individual needs at different disease stages.

Phase shuttling of Pb3O4 leads to the reconstructed β-PbO2 phase and significantly enhances the electrochemical ozone production (EOP) through water oxidation.

To summarize the targeted therapies and immunotherapy of Kirsten rat sarcoma viral oncogene homolog (KRAS)-mutant non-small cell lung cancer (NSCLC), and discuss the ongoing clinical trials.KRAS mutations occur in about 30% of patients with NSCLC and are the second most frequent genetic variation in lung cancer. It has been considered "undruggable" for 40 years until the discovery of a direct inhibitor of KRAS G12C. The promising direct KRAS G12C inhibitors such as sotorasib and MRTX849 have made a breakthrough with promising anti-tumor effects in patients with KRAS G12C-mutant advanced/metastatic NSCLC post one prior line of therapy. Following the success of immune checkpoint inhibitors (ICIs) in NSCLC, many patients harboring KRAS mutations can benefit from ICIs. However, due to disease heterogeneity, the prognosis of patients remains unsatisfactory, leaving room for personalized treatment options, such as new targeted therapies and other therapies.In this review, we aim to dissect the strategies of clinical trials in these tumors, shifting from a few chemotherapy options to targeted and immunotherapy, in the context of molecular selection of KRAS-mutant NSCLC subtypes.

The aim of this study was to understand the dynamics of multidimensional poverty and its determinants among mid-aged and older adults. We used 2011, 2013, 2015, and 2018 data from the China Health and Retirement Longitudinal Study. We utilised basic descriptive statistics, the poverty transition matrix, Kaplan–Meier estimates, and the discrete-time proportional hazards model for data analysis. From 2011 to 2018, the incidence of multidimensional poverty among mid-aged and older people basically decreased, but the average poverty intensity remained stable. Most mid-aged and older adults had transient multidimensional poverty. The longer an individual remained multidimensionally poor, the smaller the probability of exit from poverty; The longer an individual remained nonpoor after escaping from poverty, the smaller the probability of returning to poverty. As to other factors, individual characteristics, family structure, living arrangements, social capital, and living areas significantly affected the risks of multidimensional poverty exit and reentry. Based on these results, the government should implement targeted interventions for frail older adults with the identified characteristics to prevent them from persistent multidimensional poverty or return to poverty.

Additional file 1: Table S1. Information of the top 95 significant DM genetic markers. Table S2. Genotype distributions of 44 DM SNPs in CP-positive and -negative subjects.

BackgroundA better understanding of the T cells in lung cancer and their distribution across tumor-adjacent lungs and the peripheral blood is needed to improve efficacy and minimize toxicity from immunotherapy to lung cancer patients.MethodsHere, we performed CDR3β TCR sequencing of 143 samples from 21 patients with early-stage NSCLC including peripheral blood mononuclear cells, tumor, tumor edges (ResultsOur study reveals a decreasing gradient in TIL homology with the tumor-edge, adjacent lungs, and peripheral blood but no discernible distance-associated patterns of T cell trafficking within the adjacent lung itself. Furthermore, we show a decrease in pathogen-specific TCRs in regions with high T cell clonality and PD-L1 expression.ConclusionsThe exclusion in T cells at play across the lungs of patients with NSCLC may be potentially the mechanism for lung cancer occurrence.

A major goal of methodologies related to large scale gene expression analyses is to initiate comprehensive information on transcript signatures in single cells within the tissue's anatomy. Until now, this could be achieved in a stepwise experimental approach: (1) identify the majority of transcripts in a single cell (single cell transcriptome); (2) provide information on transcripts on multiple cell subtypes in a complex sample (cell heterogeneity); and (3) give information on each cell's spatial location within the tissue (zonation transcriptomics). Such genetic information will allow construction of functionally relevant gene expression maps of single cells of a given anatomically defined tissue compartment and thus pave the way for subsequent analyses, including their epigenetic modifications. Until today these aims have not been achieved in the area of cardiovascular disease research though steps toward these goals become apparent: laser capture microdissection (LCM)-based mRNA expression microarrays of atherosclerotic plaques were applied to gain information on local gene expression changes during disease progression, providing limited spatial resolution. Moreover, while LCM-derived tissue RNA extracts have been shown to be highly sensitive and covers a range of 10-16,000 genes per array/small amount of RNA, its original promise to isolate single cells from a tissue section turned out not to be practicable because of the inherent contamination of the cell's RNA of interest with RNA from neighboring cells. Many shortcomings of LCM-based analyses have been overcome using single-cell RNA sequencing (scRNA-seq) technologies though scRNA-seq also has several limitations including low numbers of transcripts/cell and the complete loss of spatial information. Here, we describe a protocol toward combining advantages of both techniques while avoiding their flaws.

Additional file 1: Table S1. The NRF1 downstream genes with a cell cycle association were identified by DAVID, KOBAS and Metascape online databases. Table S2. The overlapping NRF1 target genes in GO and Reactome analyses are detailed in the table. Table S3. The putative specific loci and the scoring of NRF1 in the promoter proximal regions of E2F1 were analyzed by the open-access database JASPAR.

This study investigated the molecular mechanism by which bronchoalveolar lavage fluid exosomes (BALF-exo) alleviated acute lung injury (ALI). BALF-exo was isolated from mice. LPS was used to induce inflammation in rat alveolar macrophages (NR8383). NR8383 cell models were treated with BALF-exo or BALF-exo loaded with miR-223-3p mimics/inhibitors, or STK39 was overexpressed in NR8383 cells before LPS and BALF-exo treatment. These cells were subjected to apoptosis, autophagy, and inflammation assays. RNA immunoprecipitation and dual-luciferase reporter assay were conducted to verify the binding between miR-223-3p and STK39. LPS-induced ALI mouse models were treated with BALF-exo loaded with miR-223-3p mimics. miR-223-3p was lowly expressed in BALF-exo from LPS-treated mice. BALF-exo loaded with miR-223-3p mimics increased viability and autophagy and decreased apoptosis and inflammation in NR8383 cell models. Inhibition of miR-223-3p in BALF-exo or overexpression of STK39 in NR8383 cells repressed the therapeutic effect of BALF-exo in LPS-treated NR8383 cells. STK39 was a target gene of miR-223-3p. miR-223-3p shuttled by BALF-exo negatively regulated the expression of STK39 in NR8383 cells. BALF-exo loaded with miR-223-3p mimics also reduced lung injuries in ALI mice. In conclusion, miR-223-3p loaded in BALF-exo alleviates ALI by targeting STK39 in alveolar macrophages.

This paper analyzes the crosstalk issues for a closed-loop active insulated gate bipolar transistor (IGBT) gate driver (AGD) based on di/dt and dv/dt detection and presents a crosstalk suppression (CTS) circuit. Closed-loop active gate drivers for IGBTs are beneficial in switching transient control by generating extra gate control signals to regulate the gate drive voltage or current with di/dt and dv/dt feedback. AGDs achieve the compensation of the IGBT nonlinearities and are independent of operating points. In a phase-leg configuration, however, AGDs produce additional crosstalk interference besides that due to reverse transfer capacitance. These two kinds of crosstalk interferences together lead to an oscillation in the IGBT gate voltage, and then a shoot-through fault may occur. An equivalent circuit model of the feedback circuit is presented to analyze and evaluate the crosstalk issues. The design and realization of a CTS circuit are presented to eliminate the crosstalk and extra gate drive losses caused by crosstalk. The experimental results substantiate the validity of the theoretical crosstalk analysis and the effectiveness of the proposed CTS circuit.

Traditional Chinese medicine prescriptions are widely believed to exert therapeutic benefits via a multiple-component and multiple-target mode. The systemic profiling of their in vitro chemicalome and in vivo metabolome is of great importance for further understanding their clinical value. Herein, an integrated strategy using ultra-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry was proposed to profile the chemicalome and metabolome of Chai-Gui Decoction. Particularly, an approach combined mass defect filter, characteristic product ion filter, and neutral loss filter was adopted to identify metabolites in plasma, urine, bile, and feces by MetabolitePilot. Consequently, a total of 174 constituents were identified or tentatively characterized and 70 metabolites that related to 21 representative structural components were matched in rat biofluids. Among them, 19 prototypes and 7 metabolites that contributed to flavonoids, monoterpenes, and phenylpropanoids were detected distribution in brain, heart, kidney, liver, lung or spleen. This study provided a generally applicable approach to comprehensive investigation on chemicalome and metabolome of traditional Chinese medicine prescriptions, and offered reasonable guidelines for further screening of quality control indicators of Chai-Gui Decoction.

We describe a new species of cave-dwelling loach, Triplophysa wulongensis sp. nov., based on specimens collected in a subterranean pool in a cave in Wulong County, Chongqing, Southwest China. The pool is connected to the Wujiang River drainage. Triplophysa wulongensis differs from its congeners by the following combination of characters: eyes present, caudal fin with 18 branched rays; posterior chamber of the air bladder degenerate; stomach U-shaped; intestine without bends or loops immediately posterior to stomach; body smooth and scaleless, and lateral line complete. The mitochondrial cytochrome b sequence differs from those of other published sequences of species of Triplophysa by 14.9–24.9% in K2P distance. Phylogenetic analysis based on cytochrome b gene sequences recovered T. wulongensis as sister taxon to all other cave-dwelling species of Triplophysa .

Zhang, Guo-Cheng, Hong, Hua-Feng, Chen, Ge-Hong, Lu, Shu-Gang, Chang, Yan-Fen (2021): Species delimitation of Hymenasplenium obliquissimum group (Aspleniaceae) in southwestern China. Phytotaxa 480 (1): 29-44, DOI: 10.11646/phytotaxa.480.1.3, URL: http://dx.doi.org/10.11646/phytotaxa.480.1.3

Additional file 1: Figure 1. Plasma TMAO concentrations in patients classified by A reason for hospitalization (sepsis vs. elective CAG) and B sepsis and different severity of CAD. Table 1. Baseline characteristics, TMAO concentrations, and outcomes of septic patients and patients admitted for elective CAG. Table 2. Plasma TMAO concentrations and nutritional indicators of patients classified by different causes of death during hospitalization. Table 3. Univariate and multivariate Cox regression analyses to investigate the relationships between TMAO, septic shock, total bilirubin, enteral intake of calories, enteral intake of protein, total calories, total protein, and non-cardiovascular death among septic patients. Table 4. Univariate and multivariate Cox regression analyses to investigate the relationships between TMAO, disease severity, antibiotic pre-treatment, nutritional risk scores, and non-CV death among septic patients. Table 5. Univariate and multivariate Cox regression analyses of factors associated with weaning success among septic patients

Additional file 6. The cross-tabulation of diagnostic performance of DECT in all the disease durations.

Additional file 3. The cross-tabulation of diagnostic performance of DECT in the early stage (≤1 yrs).

Additional file 4. The cross-tabulation of diagnostic performance of DECT in the middle stage (≤3 yrs).

Additional file 5. The cross-tabulation of diagnostic performance of DECT in the late stage (>3 yrs).

Environmental DNA (eDNA) techniques refer to utilizing the organisms’ DNA extracted from environment samples to genetically identify target species without capturing actual organisms. eDNA metabarcoding via high‐throughput sequencing can simultaneously detect multiple fish species from a single water sample, which is a powerful tool for the qualitative detection and quantitative estimates of multiple fish species. However, sequence counts obtained from eDNA metabarcoding may be influenced by many factors, of which primer bias is one of the foremost causes of methodological error. The performance of 18 primer pairs for COI, cytb, 12S rRNA, and 16S rRNA mitochondrial genes, which are all frequently used in fish eDNA metabarcoding, were evaluated in the current study. The ribosomal gene markers performed better than the protein‐coding gene markers during in silico screening, resulting in higher taxonomic coverage and appropriate barcode lengths. Four primer pairs—AcMDB07, MiFish‐U, Ve16S1, and Ve16S3—designed for various regions of the 12S and 16S rRNA genes were screened for tank metabarcoding in a case study targeting six freshwater fish species. The four primer pairs were able to accurately detect all six species in different tanks, while only MiFish‐U, Ve16S1, and Ve16S3 revealed a significant positive relationship between species biomass and read count for the pooled tank data. The positive relationship could not be found in all species within the tanks. Additionally, primer efficiency differed depending on the species while primer preferential species varied in different fish assemblages. This case study supports the potential for eDNA metabarcoding to assess species diversity in natural ecosystems and provides an alternative strategy to evaluate the performance of candidate primers before application of eDNA metabarcoding in natural ecosystems., The maintenance of fish diversity is critical for aquatic ecosystem healthy, but in order to properly evaluate this, we must ensure that the tools used are properly evaluated and that we know how to identify problems or biases. As aquatic ecosystem management is a global issue, we feel that this research will have a broad audience in the world of freshwater and marine ecosystem research.

Background Malignant peripheral nerve sheath tumours (MPNSTs) of the trachea are extremely uncommon neoplasms with unknown genetic and clinical profiles. Only individual cases have been reported in the literature to date. Case presentation Here, we present a rare case of a 61-year-old female patient with a primary MPNST of the trachea who complained of irritating cough and progressively increasing breathlessness for 4 weeks. This patient initially underwent intraluminal resection of the mass and was misdiagnosed with clear cell sarcoma. Less than a year later, the mass relapsed, and the obstructive symptoms reappeared and gradually worsened. Debulking of the endotracheal tumour mass was performed once again, and an MPNST was definitively diagnosed. Open sleeve tracheal resection and tracheoplasty were later performed with curative intent. This patient was alive without recurrence at her six-month postoperative follow-up. We also compared the clinical outcomes of previously reported cases of MPNSTs and our case. Conclusions This paper emphasizes that thoracic surgeons should be aware that malignant peripheral nerve sheath tumours of the trachea can be misdiagnosed in clinical practice and must be included in the differential diagnosis of tracheal neoplasms.

OBJECTIVE To investigate the effects of Shenqi Yangxin decoction (SQYXD) on heart function in a rat model of dilated cardiomyopathy (DCM) and its potential mechanisms. METHODS Sprague-Dawley rats were randomly divided into normal (10 rats) and DCM (150 rats) groups. DCM was induced by an intraperitoneal injection of adriamycin. Then, DCM baseline group was randomly selected sixteen DCM rats. The remaining DCM rats were randomly divided into DCM control, perindopril, metoprolol, and SQYXD groups. Cardiac function and histological analysis plus biochemical measurement of serum levels of brain natriuretic peptide (BNP), and inflammatory factors were measured. The mRNA and protein expression levels of high-mobility group box 1 (HMGB1), Toll-like receptor 4 (TLR-4), receptor for advanced glycation end products (RAGE), and nuclear factor-κB (NF-κB) were determined. Myocardial metabolism imaging was performed on the normal, SQYXD and DCM control groups to evaluate the effectiveness of treatments. RESULTS Rats in the DCM control group exhibited dilated left ventricular diameter, impaired cardiac function, disorganized sarcomere, impaired glucose metabolism, increased heart weight index, and increased levels of BNP, which were improved by treatment with SQYXD. In addition, hearts from rats in the DCM baseline group exhibited significantly higher levels of HMGB1, TLR-4, RAGE, NF-κB, tumor necrosis factor-α, interleukin-1, interleukin-6, interleukin-10, compared with the normal group. Interestingly, the mRNA level of HMGB1 in the DCM baseline group was positively correlated with that of TLR-4, RAGE, NF-κB, BNP, and LVEDD, but negatively correlated with LVEF. SQYXD inhibited the upregulation of HMGB1 expression and its downstream inflammatory factors. CONCLUSION Shenqi Yangxin decoction effectively reduced the dilated left ventricular diameter and improved heart function in dilated cardiomyopathy. The mechanisms underlying the action on DCM involve regulating the gene and protein expression of HMGB1 and its inflammatory signal pathways in the DCM rat model.

Sialic acid, the major component of edible bird's nests, was evaluated for its hypotensive and antioxidant activities. In the mesenteric arteries with complete endothelium but not with stripped off endothelium, sialic acid exhibited a dose-dependent relaxant effect suggesting a strong antihypertensive effect. In addition, sialic acid exhibited free radical elimination ability with a stronger scavenging ability for hydroxide radicals compared to DPPH radicals. In conclusion, the results presented herein provide a basis for clinical investigations into application of sialic acid as functional food for management of hypertension.

Influence of orthokeratology on corneal biomechanics is equivocal using Ocular Response Analyzer, ORA. Implementing indentation method, corneal tangent modulus was measured and monitored in short-term orthokeratology.Sixteen young subjects with refractive errors between -4D to -5D sphere and astigmatism within -1.50D were recruited. One randomly selected eye wore orthokeratology lens (treatment), and the fellow eye wore conventional rigid gas permeable lens (control). Lenses were worn for 30 and 60 minutes and one night separately with a week of washout period in between. The first two visits were randomly scheduled and before the overnight visit. Eyes were kept closed during all the lens wearing periods. Corneal radius, thickness, and biomechanics (using both ORA and an indentation device) were compared between eyes prior to each visit, and then before and after lens wear. Associations between baseline corneal biomechanics and central cornea from overnight visit were investigated.Corneal parameters were similar in each visit before lens wear. Significant corneal flattening was observed in treatment eyes, and flattening increased with wearing time. Control eyes showed no significant corneal curvature changes. Corneal resistance factor (CRF) reduced by 0.42mmHg (± 0.68mmHg) after 30 minutes of orthokeratology treatment. Corneal hysteresis (CH) reduced by 0.42mmHg (+/- 0.63mmHg) in control eyes from overnight wear. Both eyes showed stable tangent modulus, E, throughout the study. A lower CH (r = 0.51, p = 0.046) and a higher E (r = 0.53, p = 0.037) at baseline was significantly associated with greater corneal flattening along the flattest meridian in treatment eyes.Short-term orthokeratology had no significant effect on corneal tangent modulus. Changes in CH and CRF could be related to their intrinsic measurement variability. Corneal tangent modulus provided another measure of corneal biomechanics. Long-term study is required to investigate predictive role of corneal biomechanics in orthokeratology.

Objective To evaluate the therapeutic effect of Yiqi Yangyin prescription for dilated cardiomyopathy (DCM). Methods Electronic databases were searched for relevant clinical randomized controlled trials of DCM treatment using Yiqi Yangyin prescription (Shengmai and Zhigancao decoction). Databases searched included PubMed, EMBASE, the Cochrane Library, China National Knowledge Infrastructure Database, Wanfang Database, China Science and Technology Journal Database, and Chinese Biological Medicine from January 1, 1985, to October 31, 2015. After literature screening and data extraction according to previously determined exclusion criteria, data were analyzed using RevMan 5.3 and Stata 12.0. Study heterogeneity was evaluated using the I 2 test and Cochran's Q test. Egger's test was used to detect publication bias. Results Nineteen trials involving 1024 participants were included in the Meta-analysis. Of these, 15 used Shengmai (treatment group: 421 cases; control group: 344 cases) and 4 used Zhigancao decoction (treatment group: 133 cases; control group: 126 cases). A Meta-analysis demonstrated that the total curative effect was significantly improved by combining Shengmai with conventional treatment [relative risk ( RR ) = 1.32, 95% confidence intervals ( CI ) (1.22, 1.43), P SMD ) = 1.13, 95% CI (0.55, 1.70), P SMD = −0.46, 95% CI (−0.70, −0.23), P RR = 1.34, 95% CI (1.16, 1.54), P Conclusion Compared with conventional therapy, Yiqi Yangyin prescription can significantly improve the curative effect, increase left ventricular ejection fraction, and reduce left ventricular end diastolic. Thus, it can effectively improve heart function in patients with DCM.

This paper proposes an active current source gate drive (ACSD) method based on voltage controlled current source(VCCS) feedback control strategy for high-power IGBTs. Unlike the common voltage source gate drive, the proposed ACSD method provides constant drive current to charge and discharge an IGBT. With a large gate drive current, high switching speed and low switching losses can be achieved in a power converter. However, a high-current/voltage overshoot occurs. To solve this problem, a feedback current proportional to the di/dt or dv/dt signal is generated to the IGBT gate. Thus, direct control of the net gate drive current is produced. Then, the current/voltage overshoot is controlled with little sacrifice in switching time, and the switching losses are lower than that with the conventional gate drive method operating simply by gate resistance switchover. The operation principle and circuit implementation of the proposed ACSD method are presented. The experimental results from a 1200 V/800 A IGBT module verify the performance of the proposed method.

THz wave is located in the special area of transition from macro-electronics to micro-photonics in the electromagnetic spectrum, which is of great value in the fields of imaging, communication, security, biological medicine and nation defense. At present, the highly integrated solid-state light source has become one of the key problem restricting the application of this technology. We design a solid-state THz oscillation source by using based-RTD (resonant tunneling diode) electronic device for improving the property of THz source devices.

We consider the problem of choosing design parameters to minimize the probability of an undesired rare event that is described through the average of $n$ iid random variables. Since the probability of interest for near optimal design parameters is very small, one needs to develop suitable accelerated Monte-Carlo methods for estimating the objective function of interest. One of the challenges in the study is that simulating from exponential twists of the laws of the summands may be computationally demanding since these transformed laws may be non-standard and intractable. We consider a setting where the summands are given as a nonlinear functional of random variables that are more tractable for importance sampling in that the exponential twists of their distributions take a simpler form (than that for the original summands). We also study the closely related problem of estimating buffered probability of exceedance and provide the first rigorous results that relate the asymptotics of buffered probability and that of the ordinary probability under a large deviation scaling. The analogous minimization problem for buffered probability, under conditions, can be formulated as a convex optimization problem which makes it more tractable than the original optimization problem. We show that, under conditions, changes of measures that are asymptotically efficient (under the large deviation scaling) for estimating ordinary probability are also asymptotically efficient for estimating the buffered probability of exceedance. We embed the constructed importance sampling scheme in suitable gradient descent/ascent algorithms for solving the optimization problems of interest. Implementation of schemes for some examples is illustrated through computational experiments.

Glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) has been identified in multiple genome-wide association studies (GWAS) as a contributor to obesity, and GIPR knockout mice are protected against diet-induced obesity (DIO). On the basis of this genetic evidence, we developed anti-GIPR antagonistic antibodies as a potential therapeutic strategy for the treatment of obesity and observed that a mouse anti-murine GIPR antibody (muGIPR-Ab) protected against body weight gain, improved multiple metabolic parameters, and was associated with reduced food intake and resting respiratory exchange ratio (RER) in DIO mice. We replicated these results in obese nonhuman primates (NHPs) using an anti-human GIPR antibody (hGIPR-Ab) and found that weight loss was more pronounced than in mice. In addition, we observed enhanced weight loss in DIO mice and NHPs when anti-GIPR antibodies were codosed with glucagon-like peptide-1 receptor (GLP-1R) agonists. Mechanistic and crystallographic studies demonstrated that hGIPR-Ab displaced GIP and bound to GIPR using the same conserved hydrophobic residues as GIP. Further, using a conditional knockout mouse model, we excluded the role of GIPR in pancreatic β-cells in the regulation of body weight and response to GIPR antagonism. In conclusion, these data provide preclinical validation of a therapeutic approach to treat obesity with anti-GIPR antibodies.

In this paper, we study positive Toeplitz operators on the harmonic Bergman space via their Berezin transforms. We consider the Toeplitz operators with continuous harmonic symbols on the closed disk and show that the Toeplitz operator is positive if and only if its Berezin transform is nonnegative on the disk. On the other hand, we construct a function such that the Toeplitz operator with this function as the symbol is not positive but its Berezin transform is positive on the disk. We also consider the harmonic Bergman space on the upper half plane and prove that in this case the positive Toeplitz operators with continuous integrable harmonic symbols must be the zero operator.

ObjectiveTo observe the effect of Liandouqingmai recipe (Chinese herbal medicine compound preparation) on the quality of life (QOL) and inflammatory reaction of patients with coronary heart disease (CHD).MethodsA total of 101 CHD patients were randomized into two groups: treatment group (n=45) receiving standard treatment for CHD plus Liandouqingmai recipe, and control group (n=56) receiving standard treatment only. The control group contained 16 normal healthy subjects. Changes in hs-C-reactive protein (CRP), peripheral blood leucocytes (PBL), and interleukin (IL)-6 and IL-10 levels were measured. The Seattle Angina Questionnaire (SAQ) was used to determine patient QOL before and after treatment for 2 weeks.ResultsBefore treatment, SAQ scores [physical limitation (PL), angina stability (AS), angina frequency (AF), treatment satisfaction (TS), and disease perception (DP)] were not statistically different between groups. After treatment, AS and DP levels of controls were significantly increased compared with the other groups, while PL, AS, AF, TS, and DP levels of the treatment group were significantly increased compared with controls. Treatment group SAQ scores (PL, AS, AF, TS, and DP) were significantly higher than for controls. CHD patient IL-6 and IL-10 levels were significantly higher than controls. Before treatment, mean levels of IL-6, hs-CRP and PBL of the two groups were not statistically different. After treatment, mean levels of IL-6, IL-10, hs-CRP and PBL of the two groups were significantly decreased compared with their before treatment values, and levels of IL-6, hs-CRP, and PBL of the treatment group were lower than controls. Although mean IL-10 levels of both groups decreased, there was no significant difference in between-group and in-group comparisons before and after treatment. Mean levels of IL-6 and IL-10 in the normal group were lower than in CHD patients. SAQ scores of QOL were negatively associated with the inflammatory index (IL-6/IL-10), and there was a significant negative association of IL-10 with AS (r= − 0.15, P

Based on the catalytic activity of hemin, an efficient biocatalyst, an indirect capillary electrophoresis–chemiluminescence (CE-CL) detection method for phenols using a hemin–luminol–hydrogen peroxide system was developed. Through a series of static injection experiments, hemin was found to perform best in a neutral solution rather than an acidic or alkaline medium. Although halide ions such as Br− and F− could further enhance the CL signal catalyzed by hemin, it is difficult to apply these conditions to this CE-CL detection system because of the self-polymerization of hemin, as it hinders the CE process. The addition of concentrated ammonium hydroxide to an aqueous/dimethyl sulfoxide solution of hemin–luminol afforded a stable CE-CL baseline. The indirect CE-CL detection of five phenols using this method gave the following limits of detections: 4.8 × 10−8 mol/L (o-sec-butylphenol), 4.9 × 10−8 mol/L (o-cresol), 5.4 × 10−8 mol/L (m-cresol), 5.3 × 10−8 mol/L (2,4-dichlorophenol) and 7.1 × 10−8 mol/L (phenol). Copyright © 2013 John Wiley & Sons, Ltd.

In this paper, we introduce a general multilevel gradient Uzawa algorithm for symmetric saddle point systems. We compare its performance with the performance of the standard Uzawa multilevel algorithm. The main idea of the approach is to combine a double inexact Uzawa algorithm at the continuous level with a gradient type algorithm at the discrete level. The algorithm is based on the existence of a priori multilevel sequences of nested approximation pairs of spaces, but the family does not have to be stable. To ensure convergence, the process has to maintain an accurate representation of the residuals at each step of the inexact Uzawa algorithm at the continuous level. The residual representations at each step are approximated by projections or representation operators. Sufficient conditions for ending the iteration on a current pair of discrete spaces are determined by computing simple indicators that involve consecutive iterations. When compared with the standard Uzawa multilevel algorithm, our proposed algorithm has the advantages of automatically selecting the relaxation parameter, lowering the number of iterations on each level, and improving on running time. By carefully choosing the discrete spaces and the projection operators, the error for the second component of the solution can be significantly improved even when comparison is made with the discretization on standard families of stable pairs.

In this study, the Adomian's decomposition method (ADM) is applied to the STF system. This method has been tested on the STF system which is a three-dimensional system of ODE with quadratic nonlinearities. A computer based Matlab program has been developed in order to solve the STF system. Chaotic and non-chaotic behavior of the system has been analyzed graphically and finally a comparison as well as accuracy between two systems has been made in two-step sizes with detail.

This study aims to investigate the effect of Endostar injection on the rabbit ear hypertrophic scar formation and expand the use of Endostar. The rabbit ear hypertrophic scar models were established 4 weeks postoperation and were treated with Endostar injection; the control group was injected with saline, once a week, 3 times totally. At the seventh week, the scar tissue was harvested and processed with hematoxylin and eosin (HE) staining and CD34 immunohistochemistry and cell apoptosis assay. In addition, the endothelial cell was cultured and seeded on Martrigel with different concentrations of Endostar to observe the vessel tube formation. The results showed that the volume of the hypertrophic scar with Endostar injection was greatly reduced compared with what was seen in the control group; meanwhile, HE staining showed that the cell number decreased and collagen density became looser. In addition, the CD34 staining indicated that microvessel formation in the study group also decreased and cell apoptosis increased. In vitro, the addition of Endostar could reduce vessel tube formation in a dose-dependent manner. In conclusion, the Endostar is effective for hypertrophic scar inhibition and could be a potential tool to treat scars.

To investigate the characteristics of diabetic patients with foot ulcers, their health-related quality of life (HRQoL), and the link between them. The study population included 131 consecutive patients presenting in a diabetic foot clinic with a new foot ulcer between December 1, 2011, and May 1, 2012. The authors collected sociodemographic data, foot and ulcer characteristics using the Wagner Grade, and HRQoL (using the SF-36 Scale) information; 54.2% of the patients were in Wagner 2 or Wagner 3 categories. In all the 8 SF-36 subscales and in the SF-36 summary scales, the patients with diabetic foot ulcer had significantly poorer HRQoL than the general population in China ( P < .01). Their Wagner Grade had negative correlation with all the SF-36 subscales and the summary scales ( P < .05). In conclusion, new diabetic foot ulcers were already in poor condition when patients first visited the diabetic foot clinic. Concomitantly, patients had worse HRQoL compared with the general population. More effective interventions are needed to improve their self-care level and HRQoL.

A simple and effective capillary electrophoresis–chemiluminescence (CE–CL) detection system was developed based on an ultra-fast bis(2,4,6-trichlorophenyl)oxalate (TCPO) chemiluminescence (CL) reaction (0.6 s duration) that avoided overlapping peaks and peak tailing. Through a series of static injection experiments, this unusually rapid CL reaction was ascribed to the catalytic effect of imidazole in the tetrahydrofuran solvent, which has been rarely utilized in such investigations. A possible mechanism is given to explain the results. Under the optimized conditions, rhodamine 6 G (R6G) and its hydrolysis product (R6G-COOH) could be efficiently separated through electrophoresis in 7 min, with sensitive CL detection in the proposed CE–CL system. In this way, the alkaline hydrolysis of R6G was monitored, followed by estimation of relative rate constants and activation energy. This finding and application should be helpful in further study for the TCPO CL reaction, and revealed an attractive opportunity for simplifying the CE–CL system, such as in a microchip device. Copyright © 2011 John Wiley & Sons, Ltd.

This paper gives an introduction of the simulation of ultra-high-speed drilling on the copper of printed circuit board (PCB) by using finite element method (FEM). The cutting force, cutting torque and the distribution of cutting temperature are predicted. The value of cutting force is also be compared with the experimental value. The simulation results show that the experimental value of cutting force agrees well with the FEM value and the value of cutting torque is very small in the drilling process , in addition，the maximum temperature of copper comes up to 209 °C in center area and the temperature of copper chip evacuation from the spiral groove ranges from 135°C to 155 °C.

Wang, Y., Fang, H., Cao, Y., Liu, L. and Jiang, M. 2010. Jintang black goat (Capra hircas) hnRNP K: molecular cloning, sequence analysis and expression detection. J. Appl. Anim. Res., 38: 159–164. The cDNA encoding hnRNP K gene in Jintang Black goat was cloned by RT-PCR method. The entire cDNA sequence (1641 bp) contains a 1326 bp open reading frame and encodes 441 amino acids (48.709 kDa). Subsequent amino acid sequence analysis indicated that it showed a high level of sequence identity to Oryctolagus cuniculus (96.6%), Rattus norvegicus (94.5%), Bos taurus (99.7%), Homo sapiens (96.5%), Mus musculul (95.1%). Conserved domain and functional site analysis results showed that it has 3 KH domains, 3 RGG boxes, 1KNS signal, 1 acetylation site, was almost the same structure as hnRNP K proteins from other species. On the contrary, there is deletion mutation between 293 and 294, which amino acids sequence was “PGRGGRGGSRARNLPLPPPPP” in others species. Only 2 RGG-boxes (arginine-glycine- glycine boxes) ...

Background The dysfunction of baroreflex plays an important role in the pathogenesis of essential hypertension. Recent studies suggest that approximately 40% of the variation in baroreflex sensitivity (BRS) may be accounted for by genetic factors. However, only a few such genetic polymorphisms have been explicitly identified. Methods A total of 182 normotensive young men were included in the study. They were tested for the occurrence of genotypes involving nine polymorphisms in six genes that have a role in the regulation of the cardiovascular system. BRS was calculated from the spontaneous fluctuation of systolic blood pressure (SBP) and heart interval, using a sequence method. Results BRS was negatively correlated with body mass index (BMI), SBP, and family history of hypertension. Univariate analysis revealed that BRS is significantly associated with three of the polymorphisms studied. At the polymorphism T-786C in the endothelial nitric oxide (NO) synthase (eNOS) gene, the subjects with TT genotype had lower BRS than subjects carrying either the TC or the CC genotype. At the polymorphism of C-344T in the aldosterone synthase gene (CYP11B2), subjects with the TT genotype had higher BRS as compared to those with CC. At the polymorphism of T-58C in the bradykinin B2 receptor (B2R) gene, subjects with CC genotype had lower BRS as compared to subjects with TT. A multivariable linear regression analysis indicated that 16% of the BRS variation could be explained by these three polymorphisms. Conclusions Baroreflex function plays an important role in regulating blood pressure, both in the short term and in the long term. Three polymorphisms that are associated with the variation in BRS were identified in the eNOS, CYP11B2, and B2R genes, respectively; overall, they accounted for 16% of the BRS variation.

Hypertrophic scars and keloids are common problems after injury and cause functional and cosmetic deformities. A wide variety of treatments have been advocated for hypertrophic scars and keloids regression. Unfortunately, the reported efficacy has been variable. This article explores antimitotic drugs described in the literature such as steroid injection, 5-FU, mitomycin C, and bleomycin, which mainly target the fibroblasts in scar tissue, have been proposed as the effective modalities for scar treatment and scar prevention after surgery, but restricted due to possible side effects. The current accepted treatment for hypertrophic scar and keloid are combination therapy and the early treatment which could achieve better efficacy and less adverse effect.

Hypertrophic scars (HSc) have an excess of microvessels, most of which are partially or totally occluded. The mechanisms underlying microvessel endothelial cell accumulation and microvessel occlusion are poorly understood. In this study, we observed the microvessels with H&E staining and electron microscopy, and detected the cytokine expression with immunochemistry. In addition, we isolated fibroblasts and endothelial cells from both human HSc tissue and normal skin and studied their cytokine expression. Furthermore, we assayed the endothelial cell proliferation when co-cultured with normal endothelial cells and blocked with anti-VEGF and anti-bFGF neutralizing. The results revealed that more endothelial cells in HSc microvessels and the cells were swollen. The cultured HSc fibroblasts secreted significantly more while HSc endothelial cells secreted significantly less cytokines, and the same trend was found with cytokines and collagen mRNAs, which was also confirmed by immunochemistry finding. In addition, endothelial cells proliferated faster when co-cultured with HSc fibroblasts, and reduced by anti-VEGF and anti-bFGF neutralizing. This is the first report regarding the function of endothelial cells in hypertrophic scars. The hyperactivity in cytokine secretion and collagen production is largely responsible for over-proliferation and functional regression of endothelial cells, and the malfunctioning of both cell types contributes to microvessel occlusion.

Aiming at the defects of the common communication emitter recognition method, that the targets can't be recognized in the condition of low SNR, a modified communication emitter recognition method based on evidence reasoning theory is proposed. Characteristic selection and database reconstruction is applied to solve the problem of indistinct input, and the D-S algorithm of weighted averaging is applied to make inferences about signal characteristics in all levels which can avoid inefficient of data fusion. The simulation results confirm that the new method based on modified D-S theory can observably improve the performance of recognizing communication emitter for low SNR.