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Additional file 1: Table S1. List of metabolites analyzed in serum samples. Table S2. Details about the ApoE stratification of the study population. Table S3. Average neuropsychological test scores at each visit along the follow-up (i.e., V = 0, 2, 4, 7, 10 and 12 years) in cases and matched controls individuals. Values are expressed as mean (SD). Table S4. Concentrations within each study group of the fatty acid-related metabolites that were identified by lineal modeling to be associated with cognitive decline, ApoE-��4 genotype and sex. The results are expressed as mean (SD) in ��g L-1. Table S5. Concentrations within ApoE-stratified subgroups of the fatty acid-related metabolites that were identified by lineal modeling to be associated with cognitive decline, ApoE-��4 genotype and sex. The results are expressed as mean (SD) in ��g L-1. Figure S1. Pearson���s correlation analysis between metabolomics, biochemical and neuropsychological variables within the four study groups stratified according to the ApoE-��4 genotype and sex: female non-carriers (A), male non-carriers (B), female carriers (C), and male carriers (D).

The hippocampus is one of the two areas in the mammalian brain where adult neurogenesis occurs. Adult neurogenesis is well known to be involved in hippocampal physiological functions as well as pathophysiological conditions. Microtubules (MTs), providing intracellular transport, stability, and transmitting force, are indispensable for neurogenesis by facilitating cell division, migration, growth, and differentiation. Although there are several examples of MT-stabilizing proteins regulating different aspects of adult neurogenesis, relatively little is known about the function of MT-destabilizing proteins. Stathmin is such a MT-destabilizing protein largely restricted to the CNS, and in contrast to its developmental family members, stathmin is also expressed at significant levels in the adult brain, notably in areas involved in adult neurogenesis. Here, we show an important role for stathmin during adult neurogenesis in the subgranular zone of the mouse hippocampus. After carefully mapping stathmin expression in the adult dentate gyrus (DG), we investigated its role in hippocampal neurogenesis making use of stathmin knockout mice. Although hippocampus development appears normal in these animals, different aspects of adult neurogenesis are affected. First, the number of proliferating Ki-67+ cells is decreased in stathmin knockout mice, as well as the expression of the immature markers Nestin and PSA-NCAM. However, newborn cells that do survive express more frequently the adult marker NeuN and have a more mature morphology. Furthermore, our data suggest that migration in the DG might be affected. We propose a model in which stathmin controls the transition from neuronal precursors to early postmitotic neurons. (c) 2014 Wiley Periodicals, Inc. Develop Neurobiol 74: 1226-1242, 2014