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In recent years digital sensors have been successfully integrated on board Unmanned Aerial Vehicles (UAV) to assess crop vigour, vegetation coverage, and to quantify the ‘greenness’ of foliage as indirect measurements of crop nitrogen status. The classical approach of precision agriculture has involved the use of multispectral sensors onboard UAV and the development of numerous vegetation indices associated with vegetation parameters, such as the mostly used Normalized Difference Vegetation Index (NDVI). However, the main negative issue when dealing with multi and hyperspectral reflectance measuring tools is their high cost and complexity from the operational point of view. As a low-cost alternative, vegetation indices derived from Red Green Blue (RGB) cameras have been employed for remote-sensing assessment, providing data on different stress conditions and species. Digital images record information as amounts of RGB light emitted for each pixel of the image; however, the intensity of red and blue will often alter how green an image appears. To simplify the interpretation of digital colour data, recent studies have suggested converting RGB values to the more intuitive Hue, Saturation, and Brightness (HSB) colour spectrum, and then into a single measure of dark green colour, the Dark Green Color Index (DGCI). In this study, NDVI acquired by a ground-based handheld crop sensor and by a multispectral camera mounted on board a UAV has been compared with DGCI calculated from images taken with a commercial digital camera on board a UAV, trying to quantify the colour of turfgrass that had received different nitrogen (N) rates. The objectives of the trial were to study an affordable easy-to-use tool evaluating the relationship among NDVI, DGCI and leaf nitrogen content on turfgrass.