1. OsTTG1 , a WD40 repeat gene, regulates anthocyanin biosynthesis in rice
- Author
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Feng Rui, Guo Hui, Chen Can, Yinghua Pan, Zeng Yu, Xiuzhong Xia, Deng Guofu, Zhijian Xu, Li Danting, Wu Yanyan, Xinghai Yang, Junrui Wang, Zhang Zongqiong, Luo Tongping, Faqian Xiong, Nong Baoxuan, and Jie He
- Subjects
0106 biological sciences ,0301 basic medicine ,Candidate gene ,WD40 Repeats ,Mutant ,Plant Science ,Biology ,Polymorphism, Single Nucleotide ,01 natural sciences ,Anthocyanins ,03 medical and health sciences ,chemistry.chemical_compound ,WD40 repeat ,Gene Expression Regulation, Plant ,Two-Hybrid System Techniques ,Genetics ,Selection, Genetic ,Allele ,Gene ,Phylogeny ,Plant Proteins ,Oryza sativa ,fungi ,food and beverages ,Oryza ,Cell Biology ,Plants, Genetically Modified ,030104 developmental biology ,Haplotypes ,chemistry ,Anthocyanin ,Transcription Factor Gene ,Transcription Factors ,010606 plant biology & botany - Abstract
Anthocyanins play an important role in the growth of plants, and are beneficial to human health. In plants, the MYB-bHLH-WD40 (MBW) complex activates the genes for anthocyanin biosynthesis. However, in rice, the WD40 regulators remain to be conclusively identified. Here, a crucial anthocyanin biosynthesis gene was fine mapped to a 43.4-kb genomic region on chromosome 2, and a WD40 gene OsTTG1 (Oryza sativa TRANSPARENT TESTA GLABRA1) was identified as ideal candidate gene. Subsequently, a homozygous mutant (osttg1) generated by CRISPR/Cas9 showed significantly decreased anthocyanin accumulation in various rice organs. OsTTG1 was highly expressed in various rice tissues after germination, and it was affected by light and temperature. OsTTG1 protein was localized to the nucleus, and can physically interact with Kala4, OsC1, OsDFR and Rc. Furthermore, a total of 59 hub transcription factor genes might affect rice anthocyanin biosynthesis, and LOC_Os01g28680 and LOC_Os02g32430 could have functional redundancy with OsTTG1. Phylogenetic analysis indicated that directional selection has driven the evolutionary divergence of the indica and japonica OsTTG1 alleles. Our results suggest that OsTTG1 is a vital regulator of anthocyanin biosynthesis, and an important gene resource for the genetic engineering of anthocyanin biosynthesis in rice and other plants.
- Published
- 2021
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