1. Comparative study of chikungunya Virus-Like Particles and Pseudotyped-Particles used for serological detection of specific immunoglobulin M
- Author
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Pascal Dalbon, Christophe Steinbrugger, Nicolas Papageorgiou, Isabelle Leparc-Goffart, Bruno Coutard, Jérôme Martinez, Frederic Bedin, Dimitri Lavillette, Gerald Theillet, BIOMERIEUX, Unité des Virus Emergents (UVE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Systèmes Macromoléculaires et Physiopathologie Humaine (SMPH), BIOMERIEUX-Centre National de la Recherche Scientifique (CNRS), CAS Key Laboratory of Molecular Virology and Immunology [Shangai], Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA), Centre National de Référence des Arbovirus [Marseille], Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA)-Unité d'Arbovirologie [Marseille], Hôpital d'Instruction des Armées Laveran, Service de Santé des Armées-Service de Santé des Armées-Hôpital d'Instruction des Armées Laveran, Service de Santé des Armées-Service de Santé des Armées, This study was funded by BioMerieux SA, France and Agence Nationale de la Recherche et de la Technologie, France (G. Theillet's grant CIFRE ID 2015/0514)., Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Aix Marseille Université (AMU)-Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM), and BUISINE, Soline
- Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,viruses ,MESH: Chikungunya virus/physiology ,medicine.disease_cause ,Field-flow fractionation ,Antibodies, Viral ,Serology ,MESH: Antibodies, Viral/immunology ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Antibody Specificity ,Chikungunya ,[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.MHEP.ME] Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,Arbovirus ,0303 health sciences ,[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,biology ,030302 biochemistry & molecular biology ,virus diseases ,MESH: Enzyme-Linked Immunosorbent Assay ,Virus-like particles ,3. Good health ,Patient management ,MESH: Immunoglobulin M/immunology ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Chikungunya virus ,Pseudotyped virus ,Enzyme-Linked Immunosorbent Assay ,Sensitivity and Specificity ,Virus ,03 medical and health sciences ,Antigen ,Virology ,medicine ,Humans ,MESH: Serologic Tests/methods ,Serologic Tests ,MESH: Antibody Specificity ,030304 developmental biology ,MESH: Humans ,MESH: Chikungunya Fever/diagnosis ,medicine.disease ,MESH: Sensitivity and Specificity ,[SDV.BIO] Life Sciences [q-bio]/Biotechnology ,Specific igm ,Immunoglobulin M ,biology.protein ,Chikungunya Fever - Abstract
International audience; The incidence of chikungunya virus (CHIKV) infection has increased dramatically in recent decades. Effective diagnostic methods must be available to optimize patient management. IgM-capture Enzyme-Linked Immunosorbent Assay (MAC-ELISA) is routinely used for the detection of specific CHIKV IgM. This method requires inactivated CHIKV viral lysate (VL). The use of viral bioparticles such as Virus-Like Particles (VLPs) and Pseudotyped-Particles (PPs) could represent an alternative to VL. Bioparticles performances were established by MAC-ELISA; physico-chemical characterizations were performed by field-flow fractionation (HF5) and confirmed by electron microscopy. Non-purified PPs give a detection signal higher than for VL. Results suggested that the signal difference observed in MAC-ELISA was probably due to the intrinsic antigenic properties of particles. The use of CHIKV bioparticles such as VLPs and PPs represents an attractive alternative to VL. Compared to VL and VLPs, non-purified PPs have proven to be more powerful antigens for specific IgM capture.
- Published
- 2018
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