1. New evidence of the involvement of Lichtheimia corymbifera in farmer's lung disease
- Author
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Bénédicte Rognon, Stéphane Bretagne, Sandrine Roussel, Jean-Charles Dalphin, Anne-Pauline Bellanger, Laurence Millon, Charline Candido, Gabriel Reboux, Françoise Botterel, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Parasitologie-Mycologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de parasitologie [Mondor], Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), and Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 )
- Subjects
MESH: Inflammation ,MESH: Interleukin-13 ,Allergy ,MESH : RNA, Messenger ,Lichtheimia corymbifera ,Gene Expression ,MESH : Farmer's Lung ,0302 clinical medicine ,Absidia ,MESH: Up-Regulation ,MESH : Up-Regulation ,[ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology ,0303 health sciences ,Interleukin-13 ,biology ,Farmer's lung ,General Medicine ,Precipitin ,MESH : Epithelial Cells ,3. Good health ,Up-Regulation ,Infectious Diseases ,MESH : Mucorales ,MESH: Epithelial Cells ,MESH: Mucorales ,Mucorales ,Hypersensitivity pneumonitis ,MESH : Interleukin-8 ,MESH: Cell Line, Tumor ,MESH: Gene Expression ,Microbiology ,03 medical and health sciences ,Cell Line, Tumor ,MESH : Interleukin-13 ,medicine ,Humans ,Mucormycosis ,MESH : Mucormycosis ,RNA, Messenger ,MESH: RNA, Messenger ,MESH: Mucormycosis ,Pneumonitis ,MESH: Farmer's Lung ,Inflammation ,MESH : Inflammation ,MESH: Humans ,MESH : Cell Line, Tumor ,030306 microbiology ,MESH : Humans ,fungi ,Interleukin-8 ,Epithelial Cells ,biology.organism_classification ,medicine.disease ,MESH: Interleukin-8 ,Spore ,MESH : Gene Expression ,030228 respiratory system ,Immunology ,Farmer's Lung - Abstract
International audience; Farmer's lung disease (FLD) is a form of hypersensitivity pneumonitis resulting from recurrent exposure to moldy plant materials. We investigated and compared the initial response of respiratory epithelium after exposure to extracts of Sacharopolyspora rectivirgula, Lichtheimia corymbifera (formerly Absidia corymbifera), Eurotium amstelodami and Wallemia sebi. The two criteria for selection of these species were their high prevalence in the hay handled by FLD patients and the presence of high levels of specific precipitins to these molds in FLD patients' sera. Hydrosoluble extracts were prepared from spores and hyphae grown in culture under optimal conditions for each of the four species. Confluent A549 cells were inoculated with one of the four calibrated soluble extracts. Two mediators, one inflammatory (Interleukin (IL)-8) and one allergic (IL-13), were quantified using real-time PCR and ELISA assay, after four exposure periods (30 min, 2 h, 4 h and 8 h). S. rectivirgula and L. corymbifera extracts were the only ones which induced a marked upregulation of IL-8, as shown by both real-time PCR and ELISA assay 8 h after the initial contact. This study adds to the growing body of evidence that L. corymbifera should be recognized as an etiologic agent of FLD along with S. rectivirgula.
- Published
- 2010
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