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The article contains the results of chemical analysis of the water of the spring (Spring No. 6) located in Gallaorol district of Jizzakh region. The spring water sample was examined by the analytical laboratory method, and the results of physical and chemical analysis of water, in particular, pH, SiO2, dry residue, Ca2+, Mg2+, Fe, CO32-, HCO3-, SO42-, Cl, NO2, NO3, total hardness, K, indicators such as Na, Mn, Pb, Cu, Cr, Zn, Ni, Co, As, CNS, Al were determined. And coordinates of the location of Spring No. 6 were obtained. The results of the chemical analysis were compared with the state standards of Uzbekistan, and the spring water was found to be chemically suitable for domestic and drinking purposes.&nbsp

Lophiostoma khanzada-kirgizbaeva Aluth., Gafforov & K.D. Hyde, sp. nov. Index Fungorum number: IF 558817; Facesoffungi number: FoF 10528, FIGURE 2. Etymology: khanzada-kirgizbaeva (Lat.) in honour of Uzbekistan mycologist Dr. Khanzada Kirgizbaeva (1937-2007) for her contribution in studying aquatic microfungi of Uzbekistan. Saprobic on unknown hosts in terrestrial habitats. Sexual morph: Ascomata 170–450 μm high × 150–400 μm diam. (x̅ = 226 × 259 μm, n = 10), scattered to gregarious, immersed, dark brown to black, surrounded by a small blackened pseudoclypeus, globose to subglobose, ostiolate. Ostioles 120–140 μm high × 20–30 μm wide (x̅ = 130 × 25 μm, n = 5), slit-like, central, with a reduced crest and a pore-like opening. Peridium 35–80 μm wide (x̅ = 54 μm, n = 20), wider at the apex, and thinner at the base, composed of two strata, outer stratum comprising several layers of dark brown to black, somewhat flattened cells of textura angularis, fusing and indistinguishable from the host tissues, inner stratum comprising several layers with lightly pigmented cells of textura angularis. Hamathecium comprising 1–1.5 μm wide, septate, branched, pseudoparaphyses, situated between and above the asci. Asci 80–150 × 10–18 μm (x̅ = 113 × 15 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindric-clavate, with a long pedicel, rounded at the apex. Ascospores 15–25 × 6–8μm (x̅ = 22 × 7 μm, n = 50), uniseriate to bi-seriate, overlapping, initially hyaline and turning dark brown at maturity, fusiform with narrow rounded ends at immature state and turns ellipsoidal at maturity, 1–3 septate, constricted at the septum, widest at the region above or below the central septum, smooth-walled, guttulate, with mucilaginous sheath present in immature ascospores. Asexual morph: Undetermined. Material examined: Uzbekistan, Jizzakh Region, Zaamin District, Zaamin National Nature Park, Supa Plateau in the Turkestan ridge of Pamir-Alay Mountains, on a dead branch of Rosa sp., 15 July 2019, Yusufjon Gafforov, YGZ75-1_1 (TASM 6158, holotype); ibid. on a dead stem of Rosa sp., YG-Z75-1_2 (TASM 6164, paratype). Notes: Lophiostoma khanzada-kirgizbaeva (TASM 6158) is phylogenetically closest to L. paramacrostomum (MFLUCC 11-0463). Our strain L. khanzada-kirgizbaeva (TASM 6158) composed of globose to subglobose ascomata while L. paramacrostomum (MFLUCC 11-0463) composed of subglobose to conical ascomata (Thambugala et al. 2015). Lophiostoma khanzada-kirgizbaeva (TASM 6158) consists of 1–3 septate, fusiform, hyaline ascospores, which turn ellipsoidal and dark brown at maturity. Lophiostoma paramacrostomum (MFLUCC 11-0463) is characterized by 1-septate, hyaline, fusiform ascospores with narrow, acute ends. The phylogenetic analysis of combined SSU, ITS, LSU, tef 1-α, rpb 2 sequence data of Lophiostomataceae species revealed our strain Lophiostoma khanzada-kirgizbaeva (TASM 6158) within the Lophiostoma clade with good statistical support (97% ML-BS/0.91 BI-PP, FIGURE 1). Hence, confirming Lophiostoma khanzada-kirgizbaeva (TASM 6158) as a new species.

Paucispora xishanensis Aluth., Wanas. & K.D. Hyde, sp. nov. Index Fungorum number: IF 558818; Facesoffungi number: FoF 10529; FIGURE 3. Etymology: The epithet reflects the district where the specimen was collected. Saprobic on dead twigs of an unknown host. Sexual morph: Ascomata 200–340 μm high, 370–400 μm diam. (x̅ = 291 × 379 μm, n = 10), scattered to gregarious, immersed to semi-immersed, papilla erumpent through host surface, conical, flattened at the base, uni-loculate, coriaceous, black, ostiolate. Ostioles 120–140 μm high × 40–50 μm wide (x̅ = 131 × 44 μm, n = 5), slit-like, central, with a crest-like apex, broadly papillate, with a pore-like opening, filled with hyaline periphyses. Peridium 20–60 μm wide (x̅ = 39 μm, n = 20) at sides, wider at the apex, and thinner at the base, multi-layered, composed of 10–12 layers, outer layers composed of dark brown to black cells of textura angularis, indistinguishable from the host tissues, inner layer composed of hyaline cells of textura angularis. Hamathecium 1.2–1.7 µm (x̅ = 1.4 µm, n = 30) wide, composed with numerous dense, filiform, transversely septate, branched, anastomosing, cellular pseudoparaphyses situated between and above the asci, embedded in a gelatinous matrix. Asci 60–90 × 9–13 μm (x̅ = 77×11 μm, n = 30), 2–4-spored, bitunicate, fissitunicate, cylindric-clavate, apically rounded, with an ocular chamber. Ascospores 16–22 × 7–9 μm (x̅ = 20 × 8 μm, n = 50), uniseriate, partially overlapping, initially hyaline and turn dark brown at maturity, except pale end cells, ellipsoidal, straight, 1–3 septate, constricted at the central septum, widest at the region above or below the central septum, smooth-walled, guttulate, lacking a mucilaginous sheath. Asexual morph: Undetermined. Material examined: Xishan, Kunming, Yunnan, China, Woody litter of unknown host plant, 19 July 2019. DN Wanasinghe, DW1344-4_1 (HKAS 115905, holotype); ibid. DW1344-4_2 (HKAS 115906, paratype) Notes: In our phylogenetic analysis, Paucispora xishanensis (HKAS 115905) groups basal to P. versicolor (MAFF 244508). Paucispora xishanensis (HKAS 115905) is morphologically similar to P. versicolor (MAFF 244508) and P. kunmingense (MFLU 17-0558). However, P. xishanensis (HKAS 115905) has depressed conical-shaped ascomata while P. versicolor (MAFF 244508) consists of globose to subglobose ascomata (Hirayama et al. 2014; Thambugala et al. 2015) and P. kunmingense (MFLU 17-0558) consists of globose to depressed globose ascomata (Hyde et al. 2017). Paucispora xishanensis (HKAS 115905) and P. versicolor (MAFF 244508) both consist of 2-4 spored asci (Hirayama et al. 2014; Thambugala et al. 2015), while P. kunmingense (MFLU 17-0558) consists of 4-spored asci (Hyde et al. 2017). Paucispora xishanensis (HKAS 115905) consists of initially hyaline ascospores, which turn dark brown at maturity, while P. versicolor (MAFF 244508) consists of brown to reddish-brown ascospores (Hirayama et al. 2014; Thambugala et al. 2015) and P. kunmingense (MFLU 17-0558) consists of hyaline ascospores (Hyde et al. 2017). In addition, the multilocus phylogenetic analyses also confirmed the phylogenetic placement of Paucispora xishanensis isolates HKAS 115905 and HKAS 115906 within the Paucispora clade as a phylogenetically distinct species with good statistical support (100 % ML-BS/1.00 BI-PP) (FIGURE 1)., Published as part of Aluthmuhandiram, Janith V. S., Wanasinghe, Dhanushka N., Chethana, K. W. Thilini, Gafforov, Yusufjon, Saichana, Natsaran, Li, Xing Hong, Yan, Jiye & Mamarakhimov, Oybek M., 2022, Lophiostomataceae (Dothideomycetes): Introducing Lophiostoma khanzadakirgizbaeva sp. nov. and Paucispora xishanensis sp. nov., pp. 247-262 in Phytotaxa 559 (3) on page 255, DOI: 10.11646/phytotaxa.559.3.3, http://zenodo.org/record/7021719, {"references":["Hirayama, K., Hashimoto, A. & Tanaka, K. (2014) A new species, Lophiostoma versicolor, from Japan (Pleosporales, Dothideomycetes). Mycosphere 5: 411 - 417. https: // doi. org / 10.5943 / mycosphere / 5 / 3 / 3","Thambugala, K. M., Hyde, K. 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