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1 página.- Comunicación oral presentada en: XIX Jornadas sobre Producción Animal AIDA. On line. Zaragoza, España, 1-2 junio 2021., Este trabajo fue financiado por la Unión Europea (H2020 MASTER, n° 818368) a través del proyecto N° 818368 y Harbro Ltd.

1 página.- Trabajo presentado a las: XIX Jornadas sobre Producción Animal AIDA. On line. Zaragoza, España, 1-2 junio 2021., Este trabajo fue financiado por el proyecto Grupo Operativo AGUACAVALUE (www.aguacavalue.com).

1 página.- Trabajo presentado a las: XIX Jornadas sobre Producción Animal AIDA. On line. Zaragoza, España, 1-2 junio 2021., Proyecto H2020 MASTER (n° 818368), www.master-h2020.eu

1 página.- Trabajo presentado a las: XIX Jornadas sobre Producción Animal AIDA. On line. Zaragoza, España, 1-2 junio 2021., Trabajo financiado por el proyecto AGUACAVALUE (www.aguacavalue.com).

Additional file 1 Table S1. Description of the inocula in terms of rumen fermentation and microbial composition. Table S2. Effect of the early-in-life rumen microbial inoculation and age on the rumen bacteria concentration, diversity and taxonomy. Table S3. Spearman’s correlations between the bacterial taxa and the rumen function and animal performance. Table S4. Effect of early-in-life rumen microbial inoculation and age on the rumen methanogens concentration, diversity and taxonomy. Table S5. Effect of early-in-life rumen microbial inoculation and age on the rumen protozoal concentration, diversity and taxonomy. Table S6. Effect of early-in-life rumen microbial inoculation and age on the rumen anaerobic fungal concentration, diversity and taxonomy. Table S7. Descriptive statistics of the metadata used in the distance-based redundancy analyses and spearman correlations with microbial taxa abundance. Table S8. Primers used for quantitative PCR and Next Generation Sequencing. Fig. S1. Principal co-ordinates analysis illustrating the inoculation effects on the multi-kingdom rumen microbioma.

1 página.- Trabajo presentado a las: XIX Jornadas sobre Producción Animal AIDA. On line. Zaragoza, España, 1-2 junio 2021., Este trabajo fue financiado por el proyecto Grupo Operativo AGUACAVALUE (www.aguacavalue.com).

1 página.- Comunicación oral presentada en: XIX Jornadas sobre Producción Animal AIDA. On line. Zaragoza, España, 1-2 junio 2021., financiado por el proyecto AGL2017 86938-R y el contrato FPU16/01981.

1 página.-- Gut microbiology: from sequence to function (Aberdeen, Reino Unido, 16-19 junio, 2014)., Rumeh cannulation is considered the reference method for collection of representative samples of rumen digesta and is widely used in ruminant nutrition research. However, it is not feasible in lactating ewes or goats, because of adverse effects on animal performance, which obliges to depend on less invasive techniques, such as stomach tubing. The aim of this work was to study whether collecting samples through cannula or stomach tube detect the differences in the rumen bacterial community between species (sheep and goats) and diets (forage and forage plus concentrate, 1:1). Four sheep and four goats, fitted with ruminal cannula, were fed either forage or forage plus concentrate, in two 15-d periods. At the end of each period, samples of rumen digesta were taken after feeding, first by stomach tube and immediately afterwards via rumen cannula. PCRDGGE analysis of the bacterial community was carried out. The similarity dendrograrn showed that, within period, animal species was the factor driving the grouping pattem, which was more evident in samples collected through cannula. Furthermore, diversity indexes were higher for goats than for sheep only when sampling via cannula. None of the techniques detected significant variations in the bacterial diversity when comparing the diets. Our results show that stomach tubing allows the collection of a highly diverse bacterial community that is able to detect some of the effects observed when sampling through cannula. However, further studies in other microbial groups, using high-throughput sequencing tools, are needed to explore difierences in the abundances of some microbial taxa.

3 páginas, 2 tablas.-- Trabajo presentado a las XV Jornadas sobre Producción Animal AIDA (Zaragoza, 14 al 15 de mayo, 2013). Rumen cannulation is considered the most appropriate method for collection of representative samples of rumen digesta and it has been widely used in ruminant nutrition research. However, other methods such as oral stomach probing represent an alternative to allow rumen sample collection from larger populations and to reduce the cost and welfare issues associated to cannulated animals. This experiment was conducted with ruminally cannulated sheep and goats to validate the use of the stomach probing in small ruminant nutrition research. Four sheep and four goats were fed a diet consisting of alfalfa hay and concentrate (1:1). On day 15, samples of rumen digesta were taken by stomach tube and rumen cannula, before (Pre) and 4 hours after the morning feeding (Post) and rumen fermentation parameters (pH, lactate, ammonia, and total VFA concentrations) were measured. Additionally, the three main rumen microbial groups (bacteria, protozoa and methanogenic archaea) and a fibrolitic bacteria (Ruminococcus flavefaciens) were quantified by real time PCR. Overall, sampling by rumen cannula or stomach tube were able to detect similar differences in fermentation parameters and numbers of microorganisms between sheep and goats sampled before and after feeding. These results suggest that stomach probing could be a suitable method for sampling rumen digesta from small ruminants.

6 páginas, 1 tabla, 2 figuras., The aim of this work was to analyse microbial communities in two artificial rumen systems, Rusitec and single-flow continuous culture fermenters (SFCCF), fed two diets containing alfalfa hay and concentrate in the proportions of 80:20 and 20:80. Eight fermenters of each type were run for 14 days. On days 13 and 14, total effluent was collected and homogenized in a blender at low speed for 1 min and lyophilized. In order to study microbial diversity, DNA was isolated from samples (120 mg) of lyophilized effluent and the V3-4 region of the 16S rDNA gene was amplified by PCR and analysed by using single-strand-conformation polymorphisms (SSCP). Distinct clusters were observed for Rusitec and SFCCF samples. Microbial diversity, assessed using Shannon's index (H') was higher (P < 0.01) in SFCCF than in Rusitec. Within systems, samples were grouped together according to diet, but H' was not affected (P > 0.05) by diet or system. Similarity between fermenters in PCR-SSCP banding patterns was higher in the Rusitec than in SFCCF for both diets. Under the conditions of the present experiment, microbial populations were affected by diet in both systems, and microbial diversity was different in both types of fermenters., The authors wish to acknowledge the financial support received from the CICYT (Projects AGL2004-04755-C02-01 and AGL2004-04755-C02-02).