1. Trenimon-induced chromosomal damage in bone-marrow cells of six mammalian species, evaluated by the micronucleus test
- Author
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W Schmid, B E Matter, University of Zurich, and Matter, B
- Subjects
Male ,Alkylating Agents ,Erythrocytes ,10039 Institute of Medical Genetics ,Health, Toxicology and Mutagenesis ,Guinea Pigs ,Hamster ,Bone Marrow Cells ,Mutagen ,610 Medicine & health ,Biology ,medicine.disease_cause ,Chinese hamster ,Guinea pig ,Mice ,1311 Genetics ,Bone Marrow ,In vivo ,Cricetinae ,2307 Health, Toxicology and Mutagenesis ,Leukocytes ,Methods ,medicine ,1312 Molecular Biology ,Genetics ,Animals ,Toxicology and Mutagenesis ,Molecular Biology ,Cell Nucleus ,Chromosome Aberrations ,Karyotype ,Haplorhini ,biology.organism_classification ,Molecular biology ,Rats ,medicine.anatomical_structure ,10036 Medical Clinic ,Health ,Mutation ,Micronucleus test ,Macaca ,570 Life sciences ,biology ,Female ,Triaziquone ,Bone marrow ,Mutagens - Abstract
2,3,5-Tris-ethyleneiminobenzoquinone (1,4), a strong alkylating mutagen, was used in vivo to induce chromosomal damage in bone-marrow cells of the following species: Chinese and Syrian hamster, mouse, rat and guinea pig. Four different single doses between 0.031 and 0.25 mg/kg were tested. In addition, some pilot studies were performed on Rhesus monkeys. The preparations were scored for three types of aberration: micronuclei in erythrocytes (Jolly bodies), micronuclei in white cells and nuclei in lysis. A dose-dependent increase in the incidence of the three types of aberration was found in all six species with no major interspecific differences in the total percentages of nuclear anomalies. The results are in good correlation with those obtained with the cytogenetic method in Chinese hamster bone marrow. The micronucleus test appears to be a useful method offering the advantages of technical simplicity and applicability to a wide range of test mammals, irrespective of a cytologically favorable karyotype.
- Published
- 1971
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