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Additional file 1: Table S1. Study characteristics of trace metals and other oxidative stress markers.

Additional file 1: Additional Figure 1. Relative mRNA expression levels of endothelial dysfunction markers Icam1 and Vcam1 in RUPP hearts. Total RNA was collected from 30-50mg sections of cryopreserved rat hearts by a TRIsure reagent protocol. The relative levels of (A) Icam1 and (B) Vcam1 mRNA were quantified by real-time polymerase chain reaction (RT-PCR) adjusted to β-actin. Data presented as mean fold change ± SEM; n=7; unpaired t-test. Additional Figure 2. Relative mRNA expression levels of endothelial dysfunction markers Icam1 and Vcam1 in RUPP placentae. Total RNA was collected from 30-50mg sections of cryopreserved rat hearts by a TRIsure reagent protocol. The relative levels of (A) Icam1 and (B) Vcam1 mRNA were quantified by real-time polymerase chain reaction (RT-PCR) adjusted to β-actin. Data presented as mean fold change ± SEM; n=7; unpaired t-test. Additional Figure 3. Cell size and count of RUPP livers and F4/80 quantification by IHC. Formalin-fixed, paraffin-embedded rat liver tissue was sectioned at 10μm thickness and stained with H&E to visualise tissue morphology. Images of entire tissue sections were taken using an Axioscan microscope to produce virtual slides of Sham and RUPP livers. (A) Number of cells and (B) cell size of liver tissue were measured and compared. H&E rat livers were further analysed by ImageJ to detect and count cells with area 5-15μm2 (indicated by arrows) in (C) Sham and RUPP livers. Scale bar = 50μm. (D) Number of small cells in livers plotted as mean ± SEM, n=7; unpaired student’s t-test; *

© 2019 International Medical Informatics Association (IMIA) and IOS Press. Pre-eclampsia is a pregnancy condition affecting 5-10% of pregnancies, and it is the leading cause of death in pregnancy associated with increased risk of cardiovascular disease later in life. Despite research, the pathogenesis of pre-eclampsia is still poorly understood. In this paper, we investigate the overlapping pathogenic mechanisms between pre-eclampsia and adult hypertension using an integrative biomedical informatics strategy that combined text mining techniques to identify genes and proteins, with geneset analyses, generating knowledge on the pathways and mechanisms involved in these conditions. We identified several overlapping pathogenic pathways/systems including metabolic pathways, developmental biology pathways, immune system, haemostasis, tyrosine kinase pathways, extracellular matrix and oxidative stress pathways. This bioinformatics approach could be applied for investigating mechanistic pathways of other disorders.