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Additional file 1: Table S1. The comparison of demographic and clinical variables between training group and validation group of male and female patients. Table S2. The comparison of demographic variables in PSD with and without antidepressant use of male and female patients. Table S3. Tolerance of collinearity diagnosis between independent variables entered into binary logistic regression analyses for males. Table S4. Tolerance of collinearity diagnosis between independent variables entered into binary logistic regression analyses for female. Table S5. The association between BI score and PSD at 3 months in males according to stroke severity. Figure S1. A: The number of male random forest trees; B: The number of female random forest trees.

Additional file 1: Supplemental Table I. Image Acquisition Protocols. Supplemental Figure I. Z-score standardized values of closeness, betweenness and strength for all nodes.Closeness centrality refers to the inverse sum of the lengths of the shortest paths from a node to all other nodes. Betweenness centrality is the number of times a node lies on the shortest path between two other nodes. Strength centrality is the sum of all absolute values of edges connected to a node. Betweenness and closeness have demonstrated poor reliability in psychopathology network studies. Expected influence considers both positive and negative edges and may be more reliable and interpretable than strength. DepMood indicates depressed mood; Guilt, guilt feelings; Suic, suicidality; Insomn, insomnia; Workact, loss of interest in work and activities; Retard, retardation; Agit, agitation; PsyAnx, psychiatric anxiety; SomAnx, somatic anxiety; GISom, gastrointestinal somatic symptoms; GenSom, general somatic symptoms; Hypochon, hypochondriasis; WtLoss, weight loss. Supplemental Figure II. Stability of betweenness, closeness and strength in 1,000 case-dropping bootstraps. CS-C for betweenness: 0.672; CS-C for closeness: 0.75; CS-C for strength: 0.75. Supplemental Figure III. Bootstrap edge weights difference test between non-zero estimated edge-weights in the network. Bootstrapped difference tests (α = 0.05) between edge-weights that were non-zero in the network. Significant differences between two edges are indicated by black boxes, non-significant differences are indicated by grey boxes. The color of the boxes (ranging from white to blue) corresponds to the thickness of the edge. DepMood indicates depressed mood; Guilt, guilt feelings; Suic, suicidality; Insomn, insomnia; Workact, loss of interest in work and activities; Retard, retardation; Agit, agitation; PsyAnx, psychiatric anxiety; SomAnx, somatic anxiety; GISom, gastrointestinal somatic symptoms; GenSom, general somatic symptoms; Hypochon, hypochondriasis; WtLoss, weight loss.

Additional file 1. Key resources table.

Additional file 3. Oligonucleotide sequences used for qPCR.

Additional file 2. Effect of oleic acid and C75 on RAW 264.7 cell viability. Cells were treated with oleic acid (A) or C75 (B) at the indicated concentrations for 24 h. Cell viability was measured by CCK8 assays.

Additional file 1.

Three hundred and thirty-seven isolates of Salmonella Pullorum from eastern China between 1962 and 2010 were characterized for antimicrobial susceptibility (disk diffusion method), the presence of integrons (polymerase chain reaction followed by sequencing) and the ability to form biofilms (semi-quantitative adherence assay). Two hundred and fifty-eight isolates (76.6%) exhibited multiple drug resistance (MDR; resistant to at least three different classes of antimicrobials), and the level of drug resistance is increasing with time. There were three isolates (9.4%) exhibiting MDR from 1962 to 1968. MDR rates began to increase for isolates between 1970 to 1979 and 1980 to 1987 (64.6 to 78.7%). The MDR rates reached 96.6% for isolates between 1990 and 2010. Polymerase chain reaction screening for integrons showed that 75 isolates (22.3%) were positive for class 1 integrons while none were positive for class 2 integrons. All of the class 1 integron-positive isolates exhibited MDR and were more frequently resistant than the negative isolates. Two hundred and twenty isolates (65.3%) had the ability to form biofilms, and bacterial resistance levels to cefamandole, trimethoprim and trimethoprim/sulfamethoxazole were significantly higher for biofilm-positive groups than the biofilm-negative groups. Our data show that multidrug resistance is common among S. Pullorum isolated from eastern China, being more frequent after 1990 than before 1990, and the presence of class 1 integrons is associated with multidrug resistance.