Search

With its origin coming from several sources (Big Bang, stars, cosmic rays) and given its strong depletion during its stellar lifetime, the lithium element is of great interest as its chemical evolution in the Milky Way is not well understood at present. To help constrain stellar and galactic chemical evolution models, numerous and precise lithium abundances are necessary for a large range of evolutionary stages, metallicities, and Galactic volume. In the age of stellar parametrization on industrial scales, spectroscopic surveys such as APOGEE, GALAH, RAVE, and LAMOST have used data-driven methods to rapidly and precisely infer stellar labels (atmospheric parameters and abundances). To prepare the ground for future spectroscopic surveys such as 4MOST and WEAVE, we aim to apply machine learning techniques to lithium measurements and analyses. We trained a convolution neural network (CNN), coupling Gaia-ESO Survey iDR6 stellar labels (Teff, log(g), [Fe/H], and A(Li)) and GIRAFFE HR15N spectra, to infer the atm parameters and lithium abundances for ~40,000 stars. We show that the CNN properly learns the physics of the stellar labels, from relevant spectral features through a broad range of evolutionary stages and stellar parameters. The Li feature at 6707.8 A is successfully singled out by our CNN, among the thousands of lines. Rare objects such as Li-rich giants are found in our sample. This level of performance is achieved thanks to a meticulously built, high-quality, and homogeneous training sample. The CNN approach is very well adapted for the next generations of spectroscopic surveys aimed at studying (among other elements) lithium, such as the 4MIDABLE-LR/HR (4MOST Milky Way disk and bulge low- and high-resolution) surveys. In this context, the caveats of ML applications should be appropriately investigated, along with the realistic label uncertainties and upper limits for abundances., A&A, accepted 05 December 2022, 23 pages, 23 figures. Codes and trained models available at https://github.com/SamirNepal/Li\_CNN\_2022

Context. Reconstructing the Galactic evolution of lithium (Li) is the main tool used to constrain the source(s) of Li enrichment in the Galaxy. Recent results have suggested a decline in Li at supersolar metallicities, which may indicate reduced production. Aims. We exploit the unique characteristics of the Gaia-ESO Survey open star cluster sample to further investigate this issue and to better constrain the evolution of Li at high metallicity. Methods. We trace the upper envelope of Li abundance versus metallicity evolution using 18 clusters and considering members that should not have suffered any Li depletion. Results. At variance with previous claims, we do not find any evidence of a Li decrease at high metallicity. The most metal-rich clusters in the sample ([Fe/H] = ∼0.3) actually show the highest Li abundances, with A(Li) > 3.4. Our results clearly show that previous findings, which were based on field stars, were affected by selection effects. The metal-rich population in the solar neighbourhood is composed of relatively old and cool stars that have already undergone some Li depletion; hence, their measured Li does not represent the initial interstellar medium abundance, but a lower limit to it.

We analyze the oxygen abundances of a stellar sample representative of the two major Galactic populations: the thin and thick disks. The aim is to investigate the differences between members of the Galactic disks and to contribute to the understanding on the origin of oxygen chemical enrichment in the Galaxy. The analysis is based on the [O\,{\sc i}]=6300.30\,\AA~ oxygen line in HR spectra ($R\sim$52,500) obtained from the GES Survey. By comparing the observed spectra with a theoretical dataset, computed in LTE with the SPECTRUM synthesis and ATLAS12 codes, we derive the oxygen abundances of 516 FGK dwarfs for which we have previously measured carbon abundances. Based on kinematic, chemical and dynamical considerations we identify 20 thin and 365 thick disk members. We study potential trends of both subsamples in terms of their chemistry ([O/H], [O/Fe], [O/Mg], and [C/O] versus [Fe/H] and [Mg/H]), age, and position in the Galaxy. Main results are: (a) [O/H] and [O/Fe] ratios versus [Fe/H] show systematic differences between thin and thick disk stars with enhanced O abundance of thick disk stars with respect to thin disk members and a monotonic decrement of [O/Fe] with increasing metallicity, even at metal-rich regime; (b) a smooth correlation of [O/Mg] with age in both populations, suggesting that this abundance ratio can be a good proxy of stellar ages within the Milky Way; (c) thin disk members with [Fe/H]$\simeq0$ display a [C/O] ratio smaller than the solar value, suggesting a possibly outward migration of the Sun from lower Galactocentric radii., Comment: 23 pages 9 figures Accepted on 30-October 2020; to be published in AJ

The Gaia-ESO survey (GES) is now in its fifth and last year of observations and has produced tens of thousands of high-quality spectra of stars in all Milky Way components. This paper presents the strategy behind the selection of astrophysical calibration targets, ensuring that all GES results on radial velocities, atmospheric parameters, and chemical abundance ratios will be both internally consistent and easily comparable with other literature results, especially from other large spectroscopic surveys and from Gaia. The calibration of GES is particularly delicate because of (i) the large space of parameters covered by its targets, ranging from dwarfs to giants, from O to M stars; these targets have a large wide of metallicities and also include fast rotators, emission line objects, and stars affected by veiling; (ii) the variety of observing setups, with different wavelength ranges and resolution; and (iii) the choice of analyzing the data with many different state-of-the-art methods, each stronger in a different region of the parameter space, which ensures a better understanding of systematic uncertainties. An overview of the GES calibration and homogenization strategy is also given, along with some examples of the usage and results of calibrators in GES iDR4, which is the fourth internal GES data release and will form the basis of the next GES public data release. The agreement between GES iDR4 recommended values and reference values for the calibrating objects are very satisfactory. The average offsets and spreads are generally compatible with the GES measurement errors, which in iDR4 data already meet the requirements set by the main GES scientific goals.© ESO, 2017., This work was partly supported by the European Union FP7 program through ERC grant number 320360 and by the Leverhulme Trust through grant RPG-2012-541. We acknowledge the support from INAF and Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) in the form of the grant >Premiale VLT 2012>. The results presented here benefit from discussions held during the Gaia-ESO workshops and conferences supported by the ESF (European Science Foundation) through the GREAT Research Network Programme. S.F. and T.B. acknowledge the support from the New Milky Way project funded by a grant from the Knut and Alice Wallenberg foundation. C.L. gratefully acknowledges financial support from the European Research Council (ERC-CoG-646928, Multi-Pop, PI: N. Bastian). U.H. and A.J.K acknowledge support from the Swedish National Space Board (Rymdstyrelsen). The research of A.L. has been subsidized by the Belgian Federal Science Policy Office under contract No. BR/143/A2/BRASS. R.S. acknowledges support by the National Science Center of Poland through grant 2014/15/B/ST9/03981. C.A.P. is thankful for support from the Spanish Ministry of Economy and Competitiveness (MINECO) through grant AYA2014-56359-P.J.M. acknowledges support from the ERC Consolidator Grant funding scheme (project STARKEY, G.A. No. 615604). T.M. acknowledges financial support from Belspo for contract PRODEX Gaia-DPAC. S.G.S acknowledges the support by Fundacao para a Ciencia e Tecnologia (FCT) through national funds and a research grant (project ref. UID/FIS/04434/2013, and PTDC/FIS-AST/7073/2014). S.G.S. also acknowledge the support from FCT through Investigador FCT contract of reference IF/00028/2014 and POPH/FSE (EC) by FEDER funding through the program >Programa Operacional de Factores de Competitividade - COMPETE>. L.S. acknowledges support by the Ministry of Economy, Development, and Tourism's Millennium Science Initiative through grant IC120009, awarded to The Millennium Institute of Astrophysics (MAS). M.Z. acknowledges support by the Ministry of Economy, Development, and Tourism's Millennium Science Initiative through grant IC120009, awarded to The Millennium Institute of Astrophysics (MAS), by Fondecyt Regular 1150345 and by the BASAL CATA PFB-06. E.J.A. and M.T.C acknowledge the financial support from the Spanish Ministerio de Economia y Competitividad, through grant AYA2013-40611-P.S.Z. acknowledge the support from the INAF grant >PRIN INAF 2014>, >Star won't tell their ages to Gaia, Galactic Archaelogy with wide-area asterosismic>.

Investigating the physical mechanisms driving the dynamical evolution of young star clusters is fundamental to our understanding of the star formation process and the properties of the Galactic field stars. The young (~2 Myr) and partially embedded cluster Chamaeleon I is one of the closest laboratories for the study of the early stages of star cluster dynamics in a low-density environment. The aim of this work is to study the structural and kinematical properties of this cluster combining parameters from the high-resolution spectroscopic observations of the Gaia-ESO Survey with data from the literature. Our main result is the evidence of a large discrepancy between the velocity dispersion (σ = 1.14 ± 0.35 km s) of the stellar population and the dispersion of the pre-stellar cores (~0.3 km s) derived from submillimeter observations. The origin of this discrepancy, which has been observed in other young star clusters, is not clear. It has been suggested that it may be due to either the effect of the magnetic field on the protostars and the filaments or to the dynamical evolution of stars driven by two-body interactions. Furthermore, the analysis of the kinematic properties of the stellar population puts in evidence a significant velocity shift (~1 km s) between the two subclusters located around the north and south main clouds of the cluster. This result further supports a scenario where clusters form from the evolution of multiple substructures rather than from a monolithic collapse. Using three independent spectroscopic indicators (the gravity indicator γ, the equivalent width of the Li line at 6708 Å, and the Hα 10% width), we performed a new membership selection. We found six new cluster members all located in the outer region of the cluster, proving that Chamaeleon I is probably more extended than previously thought. Starting from the positions and masses of the cluster members, we derived the level of substructure Q, the surface density Σ, and the level of mass segregation Λ of the cluster. The comparison between these structural properties and the results of N-body simulations suggests that the cluster formed in a low-density environment, in virial equilibrium or a supervirial state, and highly substructured.© 2017 ESO., This work was partly supported by the European Union FP7 programme through ERC grant number 320360 and by the Leverhulme Trust through grant RPG-2012-541. We acknowledge the support from INAF and Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) in the form of the grant >Premiale VLT 2012>. This work has been partially supported by PRIN-INAF-2014. The results presented here benefited from discussions held during the Gaia-ESO workshops and conferences supported by the ESF (European Science Foundation) through the GREAT Research Network Programme. M.M. acknowledges financial support from the Italian Ministry of Education, University and Research (MIUR) through grant FIRB 2012 RBFR12PM1F, from INAF through grant PRIN-2014-14, and from the MERAC Foundation. S.G.S. acknowledges the support by Fundacao para a Ciencia e Tecnologia (FCT) through national funds and a research grant (project Ref. UID/FIS/04434/2013, and PTDC/FIS-AST/7073/2014). S.G.S. also acknowledge the support from FCT through Investigador FCT contract of reference IF/00028/2014 and POPH/FSE (EC) by FEDER funding through the program Programa Operacional de Factores de Competitividade COMPETE.

We present a family of self-consistent axisymmetric rotating globular cluster models which are fitted to spectroscopic data for NGC 362, NGC 1851, NGC 2808, NGC 4372, NGC 5927 and NGC 6752 to provide constraints on their physical and kinematic properties, including their rotation signals. They are constructed by flattening Modified Plummer profiles, which have the same asymptotic behaviour as classical Plummer models, but can provide better fits to young clusters due to a slower turnover in the density profile. The models are in dynamical equilibrium as they depend solely on the action variables. We employ a fully Bayesian scheme to investigate the uncertainty in our model parameters (including mass-to-light ratios and inclination angles) and evaluate the Bayesian evidence ratio for rotating to non-rotating models. We find convincing levels of rotation only in NGC 2808. In the other clusters, there is just a hint of rotation (in particular, NGC 4372 and NGC 5927), as the data quality does not allow us to draw strong conclusions. Where rotation is present, we find that it is confined to the central regions, within radii of R ≤ 2rh. As part of this work, we have developed a novel q-Gaussian basis expansion of the line-of-sight velocity distributions, from which general models can be constructed via interpolation on the basis coefficients., This work was partly supported by the European Union FP7 programme through ERC grant number 320360 and by the Leverhulme Trust through grant RPG-2012-541. We acknowledge the support from INAF and Ministero dell’ Istruzione, dell’ Università’ e della Ricerca (MIUR) in the form of the grant ‘Premiale VLT 2012’.

We take advantage of the Gaia-ESO Survey iDR4 bulge data to search for abundance anomalies that could shed light on the composite nature of the Milky Way bulge. The α-element (Mg, Si, and whenever available, Ca) abundances, and their trends with Fe abundances have been analysed for a total of 776 bulge stars. In addition, the aluminum abundances and their ratio to Fe and Mg have also been examined. Our analysis reveals the existence of low-α element abundance stars with respect to the standard bulge sequence in the [α/ Fe] versus [Fe/H] plane. Eighteen objects present deviations in [α/ Fe] ranging from 2.1 to 5.3σ with respect to the median standard value. Those stars do not show Mg-Al anti-correlation patterns. Incidentally, this sign of the existence of multiple stellar populations is reported firmly for the first time for the bulge globular cluster NGC 6522. The identified low-α abundance stars have chemical patterns that are compatible with those of the thin disc. Their link with massive dwarf galaxies accretion seems unlikely, as larger deviations in α abundance and Al would be expected. The vision of a bulge composite nature and a complex formation process is reinforced by our results. The approach used, which is a multi-method and model-driven analysis of high resolution data, seems crucial to reveal this complexity.

Context. Reconstructing the structure and history of young clusters is pivotal to understanding the mechanisms and timescales of early stellar evolution and planet formation. Recent studies suggest that star clusters often exhibit a hierarchical structure, possibly resulting from several star formation episodes occurring sequentially rather than a monolithic cloud collapse. Aims. We aim to explore the structure of the open cluster and star-forming region NGC 2264 (∼3 Myr), which is one of the youngest, richest and most accessible star clusters in the local spiral arm of our Galaxy; we link the spatial distribution of cluster members to other stellar properties such as age and evolutionary stage to probe the star formation history within the region. Methods. We combined spectroscopic data obtained as part of the Gaia-ESO Survey (GES) with multi-wavelength photometric data from the Coordinated Synoptic Investigation of NGC 2264 (CSI 2264) campaign. We examined a sample of 655 cluster members, with masses between 0.2 and 1.8 M and including both disk-bearing and disk-free young stars. We used T estimates from GES and g,r,i photometry from CSI 2264 to derive individual extinction and stellar parameters. Results. We find a significant age spread of 4-5 Myr among cluster members. Disk-bearing objects are statistically associated with younger isochronal ages than disk-free sources. The cluster has a hierarchical structure, with two main blocks along its latitudinal extension. The northern half develops around the O-type binary star S Mon; the southern half, close to the tip of the Cone Nebula, contains the most embedded regions of NGC 2264, populated mainly by objects with disks and ongoing accretion. The median ages of objects at different locations within the cluster, and the spatial distribution of disked and non-disked sources, suggest that star formation began in the north of the cluster, over 5 Myr ago, and was ignited in its southern region a few Myr later. Star formation is likely still ongoing in the most embedded regions of the cluster, while the outer regions host a widespread population of more evolved objects; these may be the result of an earlier star formation episode followed by outward migration on timescales of a few Myr. We find a detectable lag between the typical age of disk-bearing objects and that of accreting objects in the inner regions of NGC 2264: the first tend to be older than the second, but younger than disk-free sources at similar locations within the cluster. This supports earlier findings that the characteristic timescales of disk accretion are shorter than those of disk dispersal, and smaller than the average age of NGC 2264 (i.e., ≲ 3 Myr). At the same time, we note that disks in the north of the cluster tend to be shorter-lived (∼2.5 Myr) than elsewhere; this may reflect the impact of massive stars within the region (notably S Mon), that trigger rapid disk dispersal. Conclusions. Our results, consistent with earlier studies on NGC 2264 and other young clusters, support the idea of a star formation process that takes place sequentially over a prolonged span in a given region. A complete understanding of the dynamics of formation and evolution of star clusters requires accurate astrometric and kinematic characterization of its population; significant advance in this field is foreseen in the upcoming years thanks to the ongoing Gaia mission, coupled with extensive ground-based surveys like GES.© ESO, 2017., This work is based on data products from observations made with ESO Telescopes at the La Silla Paranal Observatory under program ID 188.B-3002. These data products have been processed by the Cambridge Astronomy Survey Unit (CASU) at the Institute of Astronomy, University of Cambridge, and by the FLAMES/UVES reduction team at INAF/Osservatorio Astrofisico di Arcetri. These data have been obtained from the Gaia-ESO Survey Data Archive, prepared and hosted by the Wide Field Astronomy Unit, Institute for Astronomy, University of Edinburgh, which is funded by the UK Science and Technology Facilities Council. This work is also based on observations made with the Spitzer Space Telescope, operated by the Jet Propulsion Laboratory, California Institute of Technology under a contract with NASA, and on data from MegaPrime/MegaCam, a joint project of CFHT and CEA/DAPNIA, at the Canada-France-Hawaii Telescope (CFHT) which is operated by the National Research Council (NRC) of Canada, the Institut National des Sciences de l'Univers of the Centre National de la Recherche Scientifique (CNRS) of France, and the University of Hawaii. The authors acknowledge useful discussions with S. Sciortino. We also thank V. Kalari, A. Klutsch, and L. A. Hillen-brand for their comments on an earlier version of this manuscript. This work was partly supported by the European Union FP7 program through ERC grant number 320360 and by the Leverhulme Trust through grant RPG-2012-541. We acknowledge the support from INAF and Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) in the form of the grant >Premiale VLT 2012>. The results presented here benefit from discussions held during the Gaia-ESO workshops and conferences supported by the ESF (European Science Foundation) through the GREAT Research Network program. The authors acknowledge support through the PRIN INAF 2014 funding scheme of the National Institute for Astrophysics (INAF) of the Italian Ministry of Education, University and Research (>The Gaia-ESO Survey>, P.I.: S. Randich). S.H.P.A. acknowledges financial support from CNPq, CAPES and Fapemig. M.T.C. acknowledges financial support from the Spanish Ministerio de Economia y Competitividad, through grant AYA2016-75931-C2-1-P.

Context. Recent spectroscopic surveys have begun to explore the Galactic disk system on the basis of large data samples, with spatial distributions sampling regions well outside the solar neighborhood. In this way, they provide valuable information for testing spatial and temporal variations of disk structure kinematics and chemical evolution. Aims. The main purposes of this study are to demonstrate the usefulness of a rigorous mathematical approach to separate substructures of a stellar sample in the abundance-metallicity plane, and provide new evidence with which to characterize the nature of the metal-poor end of the thin disk sequence. Methods. We used a Gaussian mixture model algorithm to separate in the [Mg/Fe] vs. [Fe/H] plane a clean disk star subsample (essentially at RGC< 10 kpc) from the Gaia-ESO survey (GES) internal data release 2 (iDR2). We aim at decomposing it into data groups highlighting number density and/or slope variations in the abundance-metallicity plane. An independent sample of disk red clump stars from the Apache Point Observatory Galactic Evolution Experiment (APOGEE) was used to cross-check the identified features. Results. We find that the sample is separated into five groups associated with major Galactic components; the metal-rich end of the halo, the thick disk, and three subgroups for the thin disk sequence. This is confirmed with the sample of red clump stars from APOGEE. The three thin disk groups served to explore this sequence in more detail. The two metal-intermediate and metal-rich groups of the thin disk decomposition ([Fe/H] > −0.25 dex) highlight a change in the slope at solar metallicity. This holds true at different radial regions of the Milky Way. The distribution of Galactocentric radial distances of the metal-poor part of the thin disk ([Fe/H] < −0.25 dex) is shifted to larger distances than those of the more metal-rich parts. Moreover, the metal-poor part of the thin disk presents indications of a scale height intermediate between those of the thick and the rest of the thin disk, and it displays higher azimuthal velocities than the latter. These stars might have formed and evolved in parallel and/or dissociated from the inside-out formation taking place in the internal thin disk. Their enhancement levels might be due to their origin from gas pre-enriched by outflows from the thick disk or the inner halo. The smooth trends of their properties (their spatial distribution with respect to the plane, in particular) with [Fe/H] and [Mg/Fe] suggested by the data indicates a quiet dynamical evolution, with no relevant merger events.

We study spectral diagnostics available from optical spectra with R=17000 obtained with the VLT/Giraffe HR15n setup, using observations from the Gaia-ESO Survey, on the gamma Vel young cluster, in order to determine the fundamental parameters of these stars. We define a set of spectroscopic indices, sampling TiO bands, H-alpha core and wings, and many temperature- and gravity-sensitive lines. Combined indices tau (gamma) are also defined as Teff (log g) indicators over a wide spectral-type range. H-alpha emission-line indices are also chromospheric activity or accretion indicators. A metallicity-sensitive index is also defined. These indices enable us to find a clear difference between gravities of main-sequence and pre-main-sequence stars (as well as giant stars): the (gamma,tau) diagram is thus argued to be a promising distance-independent age measurement tool for young clusters. Our indices were quantitatively calibrated by means of photometry and literature reference spectra (from UVES-POP and ELODIE 3.1 Libraries, and other datasets), over a wide range of stellar parameters. The ability of our indices to select peculiar, or other rare classes of stars is also established. Finally, our gravity determinations support the existence of an older pre-main-sequence population in the gamma Vel sky region, in agreement with the lithium depletion pattern of those stars (abridged).

We use the fundamental parameters delivered by the GES consortium in the first internal data release to select the members of $��$ Vel and Cha I among the UVES and GIRAFFE spectroscopic observations. A total of 140 $��$ Vel members and 74 Cha I members were studied. We calculated stellar luminosities through spectral energy distributions, while stellar masses were derived by comparison with evolutionary tracks. The spectral subtraction of low-activity and slowly rotating templates, which are rotationally broadened to match the $v\sin i$ of the targets, enabled us to measure the equivalent widths (EWs) and the fluxes in the H$��$ and H$��$ lines. The H$��$ line was also used for identifying accreting objects and for evaluating the mass accretion rate ($\dot M_{\rm acc}$). The distribution of $v\sin i$ for the members of $��$ Vel displays a peak at about 10 km s$^{-1}$ with a tail toward faster rotators. There is also some indication of a different $v\sin i$ distribution for the members of its two kinematical populations. Only a handful of stars in $��$ Vel display signatures of accretion, while many more accretors were detected in the younger Cha~I. Accreting and active stars occupy two different regions in a $T_{\rm eff}$-flux diagram and we propose a criterion for distinguishing them. We derive $\dot M_{\rm acc}$ in the ranges $10^{-11}$-$10^{-9} M_\odot$yr$^{-1}$ and $10^{-10}$-$10^{-7} M_\odot$yr$^{-1}$ for $��$ Vel and Cha I accretors, respectively. We find less scatter in the $\dot M_{\rm acc}-M_\star$ relation derived through the H$��$ EWs, when compared to the H$��$ $10\%W$ diagnostics, in agreement with other authors.

In this study we investigated the role of CB1 receptor signaling in angiogenesis and the therapeutic exploitation of CB1 inactivation as an antiangiogenic strategy. We started from the observation that CB1 receptor expression is induced during angiogenesis and that the endocannabinoid anandamide stimulated bFGF-induced angiogenesis in the nanomolar physiologic range. To define the functional involvement of CB1 receptor signaling during angiogenesis, 2 different strategies have been carried out: siRNA-mediated knockdown and pharmacologic antagonism of CB1 receptors. CB1 receptors inactivation resulted in the inhibition of bFGF-induced endothelial proliferation, migration, and capillary-like tube formation, through prosurvival and migratory pathways involving ERK, Akt, FAK, JNK, Rho, and MMP-2. To corroborate the potential therapeutic exploitation of CB1 blockade as an antiangiogenic strategy, we performed in vivo assays founding that CB1 blockade was able to inhibit bFGF-induced neovascular growth in the rabbit cornea assay. A relevant finding was the ability to reduce ocular pathologic neo-vascularization in mouse oxygen-induced retinopathy. These results demonstrate that CB1 signaling participates to the proliferative response elicited by proangiogenic growth factors in angiogenesis and that for this reason CB1 receptor could represent a novel target for the treatment of diseases where excessive neoangiogenesis is the underlying pathology.

The quinoline nucleus of the previously described 4-phenylquinoline-3-carboxamides NK(1) receptor ligands 7 has been transformed into either substituted or azole-(i.e., triazole or tetrazole) fused pyridine moieties of compounds 9 and 10, respectively, in order to obtain NK(1) receptor ligands showing lower molecular weight or higher hydrophilicity. The program of molecular manipulations produced NK(1) receptor ligands showing affinity in the nanomolar range. In particular, 4-methyl-1-piperazinyl derivative 9j showed an IC(50) value of 4.8nM and was proved to behave as a NK(1) antagonist blocking Sar(9)-SP-sulfone induced proliferation and migration of microvascular endothelial cells. Therefore, compound 9j has been labeled with [(11)C]CH(3)I (t(1/2)=20.4min, ²(+)=99.8%) starting from the corresponding des-methyl precursor 9i using with a radiochemical yield of about 10% (not decay corrected) and a specific radioactivity>1Ci/¼mol in order to be used as a radiotracer in next PET studies.

We present new measurements of the interstellar reddening parameter RV=AV/E(B-V) towards 185 O stars, using J, H, Ks photometry from the 2MASS project. The results are combined with data from the literature of 95 stars where RV has been derived with the same technique, 22 of which in common with our present sample from the 2MASS project catalogue. The average RV from these 258 O stars is of 3.19 +/- 0.50. All objects whose RV departs from this value by more than 2 sigma have been recognized. Ten objects have RV higher than this value and two lower. It is found that anomalous RV can scarcely be associated with anomalies in the general interstellar medium, e.g. with different behaviour in different spiral arms. They are clearly linked to local cloud effect. In the Cygnus region RV values follow the behaviour of the general interstellar medium, while in the Carina arm, in spite of the relatively larger distance, local cloud effects prevail. An explanation for this is suggested. The relatively few stars of our sample whose Hipparcos parallaxes are reliable, are found to have distances systematically smaller than the distances derived by the spectroscopic parallaxes. We argue that this effect is consistent with the recently claimed discovery of grey extinction towards OB stars.

On the basis of 125I-labeled plasminogen activator binding analysis we have found that bovine adrenal capillary endothelial cells have specific receptors for human urinary-type plasminogen activator on the cell membrane. Each cell exposes about 37,000 free receptors with a Kd of 0.8958 x 10(-9) M [corrected]. A monoclonal antibody against the 17,500 proteolytic fragment of the A chain of the plasminogen activator, not containing the catalytic site of the enzyme, impaired the specific binding, thus suggesting the involvement of a sequence present on the A chain in the interaction with the receptor, as previously shown in other cell model systems. Both the native molecule and the A chain are able to stimulate endothelial cell motility in the Boyden chamber, when used at nanomolar concentrations. The use of the same monoclonal antibody that can inhibit ligand-receptor interaction can impair the plasminogen activator and A-chain-induced endothelial cell motility, suggesting that under the conditions used in this in vitro model system, the motility of bovine adrenal capillary endothelial cells depends on the specific interaction of the ligand with free receptors on the surface of endothelial cells.

Context. Open clusters are recognised as excellent tracers of Galactic thin-disc properties. At variance with intermediate-age and old open clusters, for which a significant number of studies is now available, clusters younger than ≲150 Myr have been mostly overlooked in terms of their chemical composition until recently (with few exceptions). On the other hand, previous investigations seem to indicate an anomalous behaviour of young clusters, which includes (but is not limited to) slightly sub-solar iron (Fe) abundances and extreme, unexpectedly high barium (Ba) enhancements. Aims. In a series of papers, we plan to expand our understanding of this topic and investigate whether these chemical peculiarities are instead related to abundance analysis techniques. Methods. We present a new determination of the atmospheric parameters for 23 dwarf stars observed by the Gaia-ESO survey in five young open clusters (τ < 150 Myr) and one star-forming region (NGC 2264). We exploit a new method based on titanium (Ti) lines to derive the spectroscopic surface gravity, and most importantly, the microturbulence parameter. A combination of Ti and Fe lines is used to obtain effective temperatures. We also infer the abundances of Fe I, Fe II, Ti I, Ti II, Na I, Mg I, Al I, Si I, Ca I, Cr I, and Ni I. Results. Our findings are in fair agreement with Gaia-ESO iDR5 results for effective temperatures and surface gravities, but suggest that for very young stars, the microturbulence parameter is over-estimated when Fe lines are employed. This affects the derived chemical composition and causes the metal content of very young clusters to be under-estimated. Conclusions. Our clusters display a metallicity [Fe/H] between +0.04 ± 0.01 and +0.12 ± 0.02; they are not more metal poor than the Sun. Although based on a relatively small sample size, our explorative study suggests that we may not need to call for ad hoc explanations to reconcile the chemical composition of young open clusters with Galactic chemical evolution models.

Investigating the chemical homogeneity of stars born from the same molecular cloud at virtually the same time is very important for our understanding of the chemical enrichment of the interstellar medium and with it the chemical evolution of the Galaxy. One major cause of inhomogeneities in the abundances of open clusters is stellar evolution of the cluster members. In this work, we investigate variations in the surface chemical composition of member stars of the old open cluster M67 as a possible consequence of atomic diffusion effects taking place during the main-sequence phase. The abundances used are obtained from high-resolution UVES/FLAMES spectra within the framework of the Gaia-ESO Survey. We find that the surface abundances of stars on the main sequence decrease with increasing mass reaching a minimum at the turn-off. After deepening of the convective envelope in subgiant branch stars, the initial surface abundances are restored. We found the measured abundances to be consistent with the predictions of stellar evolutionary models for a cluster with the age and metallicity of M67. Our findings indicate that atomic diffusion poses a non-negligible constraint on the achievable precision of chemical tagging methods.

Context. Multiple stellar systems play a fundamental role in the formation and evolution of stellar populations in galaxies. Recent and ongoing large ground-based multi-object spectroscopic surveys significantly increase the sample of spectroscopic binaries (SBs) allowing analyses of their statistical properties. Aims. We investigate the repeated spectral observations of the Gaia-ESO Survey internal data release 5 (GES iDR5) to identify and characterise SBs with one visible component (SB1s) in fields covering mainly the discs, the bulge, the CoRot fields, and some stellar clusters and associations. Methods. A statistical χ 2 -test is performed on spectra of the iDR5 subsample of approximately 43 500 stars characterised by at least two observations and a signal-to-noise ratio larger than three. In the GES iDR5, most stars have four observations generally split into two epochs. A careful estimation of the radial velocity (RV) uncertainties is performed. Our sample of RV variables is cleaned from contamination by pulsation- and/or convection-induced variables using Gaia DR2 parallaxes and photometry. Monte-Carlo simulations using the SB9 catalogue of spectroscopic orbits allow to estimate our detection efficiency and to correct the SB1 rate to evaluate the GES SB1 binary fraction and its relation to effective temperature and metallicity. Results. We find 641 (resp., 803) FGK SB1 candidates at the 5σ (resp., 3σ) level. The maximum RV differences range from 2.2 km s−1 at the 5σ confidence level (1.6 km s−1 at 3σ) to 133 km s−1 (in both cases). Among them a quarter of the primaries are giant stars and can be located as far as 10 kpc. The orbital-period distribution is estimated from the RV standard-deviation distribution and reveals that the detected SB1s probe binaries with log P[d] / 4. We show that SB1s with dwarf primaries tend to have shorter orbital periods than SB1s with giant primaries. This is consistent with binary interactions removing shorter period systems as the primary ascends the red giant branch. For two systems, tentative orbital solutions with periods of 4 and 6 d are provided. After correcting for detection efficiency, selection biases, and the present-day mass function, we estimate the global GES SB1 fraction to be in the range 7–14% with a typical uncertainty of 4%. A small increase of the SB1 frequency is observed from K- towards F-type stars, in agreement with previous studies. The GES SB1 frequency decreases with metallicity at a rate of (−9 ± 3)% dex−1 in the metallicity range −2.7 ≤ [Fe/H] ≤ +0.6. This anticorrelation is obtained with a confidence level higher than 93% on a homogeneous sample covering spectral types FGK and a large range of metallicities. When the present-day mass function is accounted for, this rate turns to (−4 ± 2)% dex−1 with a confidence level higher than 88%. In addition we provide the variation of the SB1 fraction with metallicity separately for F, G, and K spectral types, as well as for dwarf and giant primaries

Endostatin, a peptide derived from proteolysis of collagen XVIII, is an endogenous inhibitor of angiogenesis and tumor growth. We have synthesized five peptide fragments designed to cover the whole length of the endostatin molecule (containing 40-50 amino acids each) with the aim of exploring the possibility that a specific sequence within the molecule might be responsible for its antiangiogenic effects.The five peptide sequences, termed fragments I, II, III, IV, and IVox, the latter bearing the original disulfide bond Cys(135)-Cys(165), were investigated for their effects on cultured endothelial cells, on enzyme activities related to angiogenesis, on tube formation in three-dimensional gel matrices, in vivo in the avascular rabbit cornea assay, and in an experimental tumor burden paradigm.Both the fragment covering the COOH-terminal endostatin region, fragment IV, and particularly fragment IVox, retained the angioinhibitory effects of endostatin. Fragment IVox strongly inhibited endothelial cell migration and proliferation, in vitro tube formation, and in vivo angiogenesis, displaying a potency comparable with that of endostatin. When tested in vivo on tumor growth, fragment IVox demonstrated to be more effective than full-length endostatin. Unexpectedly, fragment III exhibited proangiogenic activity, increasing endothelial cell migration, producing neovascularization to an extent similar to that of vascular endothelial growth factor, and enhancing the angiogenic response to vascular endothelial growth factor in the cornea assay. Peptides encompassing the NH(2)-terminal region of endostatin (fragments I and II) had negligible effects on angiogenesis.In view of these results, which show strikingly distinct profiles of endostatin fragments, we propose that the amino acid sequence of endostatin contains both angiosuppressive and angiostimulatory domains.

We attempt to determine the relative fraction of stars that have undergone significant radial migration by studying the orbital properties of metal-rich ([Fe/H]$>0.1$) stars within 2 kpc of the Sun using a sample of more than 3,000 stars selected from iDR4 of the Gaia-ESO Survey. We investigate the kinematic properties, such as velocity dispersion and orbital parameters, of stellar populations near the sun as a function of [Mg/Fe] and [Fe/H], which could show evidence of a major merger in the past history of the Milky Way. This was done using the stellar parameters from the Gaia-ESO Survey along with proper motions from PPMXL to determine distances, kinematics, and orbital properties for these stars to analyze the chemodynamic properties of stellar populations near the Sun. Analyzing the kinematics of the most metal-rich stars ([Fe/H]$>0.1$), we find that more than half have small eccentricities ($e7$ kpc. We find that the highest [Mg/Fe], metal-poor populations have lower vertical and radial velocity dispersions compared to lower [Mg/Fe] populations of similar metallicity by $\sim10$ km s$^{-1}$. The median eccentricity increases linearly with [Mg/Fe] across all metallicities, while the perigalacticon decreases with increasing [Mg/Fe] for all metallicities. Finally, the most [Mg/Fe]-rich stars are found to have significant asymmetric drift and rotate more than 40 km s$^{-1}$ slower than stars with lower [Mg/Fe] ratios. While our results cannot constrain how far stars have migrated, we propose that migration processes are likely to have played an important role in the evolution of the Milky Way, with metal-rich stars migrating from the inner disk toward to solar neighborhood and past mergers potentially driving enhanced migration of older stellar populations in the disk.

Context. We describe the atomic and molecular data that were used for the abundance analyses of FGK-type stars carried out within the Gaia-ESO Public Spectroscopic Survey in the years 2012 to 2019. The Gaia-ESO Survey is one among several current and future stellar spectroscopic surveys producing abundances for Milky-Way stars on an industrial scale.Aims. We present an unprecedented effort to create a homogeneous common line list, which was used by several abundance analysis groups using different radiative transfer codes to calculate synthetic spectra and equivalent widths. The atomic data are accompanied by quality indicators and detailed references to the sources. The atomic and molecular data are made publicly available at the CDS.Methods. In general, experimental transition probabilities were preferred but theoretical values were also used. Astrophysical gf-values were avoided due to the model-dependence of such a procedure. For elements whose lines are significantly affected by a hyperfine structure or isotopic splitting, a concerted effort has been made to collate the necessary data for the individual line components. Synthetic stellar spectra calculated for the Sun and Arcturus were used to assess the blending properties of the lines. We also performed adetailed investigation of available data for line broadening due to collisions with neutral hydrogen atoms.Results. Among a subset of over 1300 lines of 35 elements in the wavelength ranges from 475 to 685 nm and from 850 to 895 nm, we identified about 200 lines of 24 species which have accurate gf-values and are free of blends in the spectra of the Sun and Arcturus. For the broadening due to collisions with neutral hydrogen, we recommend data based on Anstee-Barklem-O'Mara theory, where possible. We recommend avoiding lines of neutral species for which these are not available. Theoretical broadening data by R.L. Kurucz should be used for ScII, TiII, and YII lines; additionally, for ionised rare-earth species, the Unsold approximation with an enhancement factor of 1.5 for the line width can be used.Conclusions. The line list has proven to be a useful tool for abundance determinations based on the spectra obtained within the Gaia-ESO Survey, as well as other spectroscopic projects. Accuracies below 0.2 dex are regularly achieved, where part of the uncertainties are due to differences in the employed analysis methods. Desirable improvements in atomic data were identified for a number of species, most importantly AlI, SI, and CrII, but also NaI, SiI, CaII, and NiI.

The plasminogen activator system is known to play a crucial role in the angiogenesis process by modulating the adhesive properties of endothelial cells to the extracellular matrix and cell-cell interaction. In the present study, we demonstrated that the urokinase-type plasminogen activator (u-PA) induced neovascular growth in the avascular rabbit cornea and dose-dependently promoted growth, chemotaxis, and matrix invasion of cultured endothelial cells. Interaction between u-PA and its receptor appears to be mandatory for the angiogenic effect of u-PA because monoclonal antibodies anti-u-PA and anti-u-PA receptor (u-PAR) blocked the proangiogenic effects of u-PA at the endothelial cell level. We then assessed the signaling pathway activated in endothelial cells by u-PA. u-PAR activation by u-PA produced de novo synthesis of diacylglycerol (DAG) from glucose by a cytochalasin B-inhibitable mechanism, indicating the involvement of a specific glucose transporter (GLUT). Endothelial cells expressed GLUT2, whose activation was tyrosine kinase-dependent and protein kinase C (PKC)-independent. The increase of glucose uptake led to DAG production, which resulted in PKC activation/translocation. Impairment of u-PAR availability by monoclonal antibodies and by antisense oligonucleotides (aODN) against u-PAR mRNA inhibited glucose uptake, DAG neosynthesis, and PKC activation, resulting in the blockade of endothelial cell proliferation, chemotaxis, and chemoinvasion. These data suggest that u-PAR activation consequent to the binding of u-PA can be regarded as an "angiogenic switch" and disclose the possibility that an anti-u-PAR aODN strategy may efficiently target endothelial cell function to control angiogenesis in vivo.

Context. TheGaia-ESO Public Spectroscopic Survey using FLAMES at the VLT has obtained high-resolution UVES spectra for a large number of giant stars, allowing a determination of the abundances of the key chemical elements carbon and nitrogen at their surface. The surface abundances of these chemical species are known to change in stars during their evolution on the red giant branch (RGB) after the first dredge-up episode, as a result of the extra mixing phenomena.Aims. We investigate the effects of thermohaline mixing on C and N abundances using the first comparison between theGaia-ESO survey [C/N] determinations with simulations of the observed fields using a model of stellar population synthesis.Methods. We explore the effects of thermohaline mixing on the chemical properties of giants through stellar evolutionary models computed with the stellar evolution code STAREVOL. We include these stellar evolution models in the Besançon Galaxy model to simulate the [C/N] distributions determined from the UVES spectra of theGaia-ESO survey and to compare them with the observations.Results. Theoretical predictions including the effect of thermohaline mixing are in good agreement with the observations. However, the field stars in theGaia-ESO survey with C and N abundance measurements have a metallicity close to solar, where the efficiency of thermohaline mixing is not very large. The C and N abundances derived by theGaia-ESO survey in open and globular clusters clearly show the impact of thermohaline mixing at low metallicity, which explains the [C/N] value observed in lower mass and older giant stars. Using independent observations of carbon isotopic ratio in clump field stars and open clusters, we also confirm that thermohaline mixing should be taken into account to explain the behaviour of12C/13C as a function of stellar age.Conclusions. Overall, the current model including thermohaline mixing is able to reproduce very well the C and N abundances over the whole metallicity range investigated by theGaia-ESO survey data.

Pismis 18 is a moderately populated, intermediate-age open cluster located within the solar circle at a Galactocentric distance of about 7 kpc. Few open clusters have been studied in detail in the inner disc region before the Gaia-ESO Survey. New data from the Gaia-ESO Survey allowed us to conduct an extended radial velocity membership study as well as spectroscopic metallicity and detailed chemical abundance measurements for this cluster. Gaia-ESO Survey data for 142 potential members, lying on the upper MS and on the red clump, yielded radial velocity measurements, which, together with proper motion measurements from the Gaia DR2, were used to determine the systemic velocity of the cluster and membership of individual stars. Photometry from Gaia DR2 was used to re-determine cluster parameters based on high confidence member stars only. Cluster abundance measurements of six radial-velocity member stars with UVES high-resolution spectroscopy are presented for 23 elements. According to the new estimates, based on high confidence members, Pismis 18 has an age of $700^{+40}_{-50}$ Myr, interstellar reddening of E(B-V) = $0.562^{+0.012}_{-0.026}$ mag and a de-reddened distance modulus of $DM_0 = 11.96^{+0.10}_{-0.24}$ mag. The median metallicity of the cluster (using the six UVES stars) is [Fe/H] = $+0.23 \pm 0.05$ dex, with [$\alpha$/Fe]= $0.07 \pm 0.13$ and a slight enhancement of s- and r- neutron-capture elements. With the present work, we fully characterized the open cluster Pismis 18, confirming its present location in the inner disc. We estimated a younger age than the previous literature values and gave, for the first time, its metallicity and its detailed abundances. Its [$\alpha$/Fe] and [s-process/Fe], both slightly super-solar, are in agreement with other inner-disc open clusters observed by the Gaia-ESO survey. [abridged]

Context. In the era of large high-resolution spectroscopic surveys such as Gaia-ESO and APOGEE, high-quality spectra can contribute to our understanding of the Galactic chemical evolution by providing abundances of elements that belong to the different nucleosynthesis channels, and also by providing constraints to one of the most elusive astrophysical quantities: stellar age. Aims. Some abundance ratios, such as [C/N], have been proven to be excellent indicators of stellar ages. We aim at providing an empirical relationship between stellar ages and [C/N] using open star clusters, observed by the Gaia-ESO and APOGEE surveys, as calibrators. Methods. We used stellar parameters and abundances from the Gaia-ESO Survey and APOGEE Survey of the Galactic field and open cluster stars. Ages of star clusters were retrieved from the literature sources and validated using a common set of isochrones. We used the same isochrones to determine for each age and metallicity the surface gravity at which the first dredge-up and red giant branch bump occur. We studied the effect of extra-mixing processes in our sample of giant stars, and we derived the mean [C/N] in evolved stars, including only stars without evidence of extra mixing. By combining the Gaia-ESO and APOGEE samples of open clusters, we derived a linear relationship between [C/N] and (logarithmic) cluster ages. Results. We apply our relationship to selected giant field stars in the Gaia-ESO and APOGEE surveys. We find an age separation between thin- and thick-disc stars and age trends within their populations, with an increasing age towards lower metallicity populations. Conclusions. With this empirical relationship, we are able to provide an age estimate for giant stars in which C and N abundances are measured. For giant stars, the isochrone fitting method is indeed less sensitive than for dwarf stars at the turn-off. Our method can therefore be considered as an additional tool to give an independent estimate of the age of giant stars. The uncertainties in their ages is similar to those obtained using isochrone fitting for dwarf stars., Includes STFC and ERC funding.

Red giant stars are perhaps the most important type of stars for Galactic and extra-galactic archaeology: they are luminous, occur in all stellar populations, and their surface temperatures allow precise abundance determinations for many different chemical elements. Yet, the full star formation and enrichment history of a galaxy can be traced directly only if two key observables can be determined for large stellar samples - age and chemical composition. While spectroscopy is a powerful method to analyse the detailed abundances of stars, stellar ages are the "missing link in the chain", since they are not a direct observable. However, spectroscopy should be able to estimate stellar masses, which for red giants directly infer ages provided their chemical composition is known. Here we establish a new empirical relation between the shape of the hydrogen line in the observed spectra of red giants and stellar mass determined from asteroseismology. The relation allows to determine stellar masses and ages with the accuracy of 10-15%. The method can be used with confidence for stars in the following range of stellar parameters: 4000 < Teff < 5000 K, 0.5 < log g < 3.5, -2.0 < [Fe/H] < 0.3, and luminosities log L/LSun < 2.5. Our analysis provides observational evidence that the Halpha spectral characteristics of red giant stars are tightly correlated with their mass and therefore their age. We also show that the method samples well all stellar populations with ages above 1 Gyr. Targeting bright giants, the method allows to obtain simultaneous age and chemical abundance information far deeper than would be possible with asteroseismology, extending the possible survey volume to remote regions of the Milky Way and even to neighbouring galaxies like Andromeda or the Magellanic Clouds already with present instrumentation, like VLT and Keck facilities.