1. DcPI2 combine DcNAC56 promoter contributes to petal development based on ABCE MADS box gene expression patterns in Dianthus chinensis
- Author
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Xiaoni Zhang, Qijian Wang, Shengnan Lin, Quanshu Wu, Dandan Li, Zhen Zhang, Mouliang Li, Luke Tembrock, Zhiqiang Wu, Caiyun Wang, Mohammed Bendahmane, Manzhu Bao, and Xiaopeng Fu
- Subjects
fungi ,food and beverages - Abstract
Dianthus chinensis is a widely used landscaping plant with high commercial value. Understanding the molecular mechanisms responsible for floral organ formation can help to improve the traits (e.g.,, petal size, floral organ shape, etc.) of D. chinensis. However, there has no comprehensive study of ABCE MADS-box genes in D. chinensis. In this study, we identified 15 ABCE MADS-box genes of D. chinensis. The expression and interactions patterns among ABCE MADS-box genes were analysis by in situ, qRT-PCT, Yeast two‐hybrid and bimolecular fluorescent complimentary experiments. In different stages of petal development, the expression of DcNAC56 changes with DcPI2. The overexpression of DcPI2 in D. chinensis resulted in high expression levels of DcNAC56. 35S: DcPI2 transgenic plants had a larger petal size with petals expansion. Furthermore, we observed that the DcPI2 protein can bind to the DcNAC56 promoter in D. chinensis. On the basis of these results, we propose that spatiotemporal expression of class ABCE genes and different combinations between class ABCE proteins might regulate floral organ specification. And DcPI2 can regulate the expansion of petals by binding to DcNAC56 involved in cell proliferation and differentiation. These results provide new insights into the improving cultivars of Dianthus by change floral traits.
- Published
- 2022
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