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Additional file 5: Fig. S5. Comparison of the growth of transgenic (StAPI5-OE) and non-transgenic potato plants (Wild-type) at the same developmental stages (8-week-old plants).

Additional file 6: Table S1. Values of gas exchange, chlorophyll fluorescence parameters and morphological traits in the resistant cv. Degima, transgenic, and Wilde-type cv. Desiree inoculated with PVA (R/PVA, StAPI5-OE/PVA, and WT/PVA, respectively), PVY (R/PVY, StAPI5-OE/PVY, and WT/PVY), buffer (R/Mock, StAPI5-OE/Mock, and WT/Mock), and without inoculation (R, StAPI5-OE, and WT). The number of replicates in each plant for each treatment was shown as Rep. No. The variables were measured in 21-day-old plants in the growth chamber. Values represent the mean (± SE) of the replicates per treatment. Values within the same row followed by the same letter (s) are not significantly different (Duncan’s multiple range test, P

Additional file 1: Figure S1. The DNA sequencing result of StAPI5 gene in the expression vector pRI201-AN using vector-specific primer M13 forward by Macrogen company using the Sanger technique.

Additional file 2: Fig. S2. PCR analysis of DNA isolated from transgenic plants with CaMV 35 S forward primer (35S-F) and StAPI5 gene reverse specific primer (StAPI5-R) to verify the insertion of the target gene into the potato genome. The expected band length is 754-bp. C: Negative control with non-transgenic potato plant (wild-type); P: positive control PCR reaction with plasmid as template; Lanes no. 1–10: DNA from independent transgenic potato plants and M: 1 kb DNA Ladder.

Additional file 7: Table S2. Sequences of the gene-specific, coat protein of PVY and PVA, and internal control primers.

Additional file 3: Fig. S3. Southern blot analysis of the independent transgenic potato plants overexpressing StAPI5 gene (right) in this image and one other studied gene (left) to confirm the transgene integration into the potato genome and to estimate the transgene copy number. P: the pRI-AN201 binary vector containing the StAPI5 gene (positive control); lanes 1 and 2: independent transgenic potato plants; C: non-transformed potato (negative control)

Additional file 1: Table S1. Primers used for cDNA amplification and qRT-PCR