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Pnemomediastinum is defined as the presence of aberrant air in the mediastinum, also known as mediastinal emphysema. Significant pneumomediastinum is rare, particularly in term neonates. It is usually related to the different predisposing factors such as birth injury, meconium aspiration, assisted ventilation or infectious diseases although it may occur idiopathically. Pneumomediastinum is one of the important causes of respiratory distress syndrome that should not be overlook in term neonates. The elevation of thymic lobes off the heart on plain view and the presence of retrosternal air collection on lateral view are crucial signs on chest x-rays that need to be assessed cautiously by radiologists and neonatologists. We report two cases of pneumomediastinum in term infants who presented with respiratory distress. One case was diagnosed as pneumomediastinum and assisted ventilation was supposed as a predisposing factor, the other case was diagnosed as spontaneous pneumomediastinum without underlying lung disease or predisposing factors. The definitive diagnosis was made by combination plain and lateral chest x-rays without further imaging method was required. Both patients received conservative treatment with close observation clinically and radiologically and were discharged after several days in good condition.

Cryptotia is a rare congenital auricular deformity among Caucasians but more common in Asians. Various operative techniques have been described, such as skin graft, V-Y plasty, Z plasty, and islands skin flap. Among those techniques, V-Y plasty has many advantages and is indicated for severe cases. However, several problems remain when this method is adopted, such as conspicuous scars and lowered hairline. To overcome these problems, the authors have developed a new technique, multiple V-Y advancement modification, which stitches the skin flaps together to a tight, discrete central focal point. This technique has the advantage of hiding scars behind the earlobe, providing enough skin for the deformed areas, minimizing the condition of the hairline being lowered and auriculocephalic sulcus depth.

Anterolateral thigh flap (ALT flap) is first found and used by Song in 1984. Classified in the perforator type flaps, ALT flaps is blood supplied by the perforators of the descending branch of the lateral circumflex thigh artery. The flap is the first of choice in many cases, those need a large soft tissue for reconstruction, especially in the cervico facial area thanks to many advantages of the flap: constants and relievable anatomic structure, large blood supply, flexible in use as well as other advantages. From 9.2016 to 10. 2020, 26 ALT flaps are dissected in fresh and Formalin cavades of mature Viet people for the anatomic research and 34 ALT flaps used for patients with soft tissue defects in cervicofacial area. The outcome is considerable with the main following conclusions: - ALT flap is constant and reliable in anatomy. - The flap is harvested relatively easy in clinical practice. - The use of the ALT flap is flexible with good result in reconstruction of soft tissue defects in cervico facial area.

Multiple symmetric lipomatosis (MSL) is a rare disease associated with metabolic disorders and alcoholism. In this report, we describe the clinical outcome and surgical protocol for eight men with Madelung collar who underwent treatment in a craniofacial and plastic surgery facility in Hanoi, Vietnam, between May 2018 and December 2020. We discuss the patients' clinical symptoms, subclinical signs, epidemiology, magnetic resonance imaging, computerized tomography, surgical protocol, complications, and postoperative indicators that we collected and evaluated. Each patient underwent surgery in two stages (i.e., in supine and prone positions). We injected a tumescent solution 10 min before the incision. Two surgeons performed each operation simultaneously. After surgery, we followed the patients for 6-27 months. All of the patients had a history of long-time alcohol abuse and had associated comorbidities that included liver disease, blood disorders, restricted neck movement, and orthopnea. We did not have to open the trachea or transfuse blood during any of the surgical procedures. Postoperatively, all patients were satisfied with their functional results and aesthetic appearance. One patient experienced a recurrence of his MSL. We believe our surgical protocol provides optimal results for patients with MSL and Madelung collar.

Chitinases from the genus Trichoderma fungi are mainly responsible for their anti-fungal activities, which allow them to become the most widely used fungal biocontrol. Therefore, several Trichoderma chitinases have been cloned and expressed to facilitate their production and applications. A previous study of the same authors has characterized an endochitinase from a relatively novel Trichoderma spp., Trichoderma asperellum. To produce this enzyme more economically and efficiently, we reported the synthesis and expression of its synthetic encoding gene in the Escherichia coli M15 strain and established the optimal conditions for preparative scale production of the enzyme in its functional form. By lowering the induction temperatures, we observed substantial improvement in the expression levels of the active enzyme. At 30 oC and 0.5 mM IPTG induction, 1 L of cells yielded approximately 80 - 100 mg of soluble protein, accounting for about 9-11 % of total soluble protein. This figure may be an underestimation of the actual yield, as deduced from the SDS-PAGE data. The recombinant enzyme can be retrieved by simple repeated freezing and thawing cycles and purified to near homogeneity using Ni-NTA chromatography. The purified enzyme showed in vitro colloidal chitin hydrolysis activity. These results could be scaled up to produce soluble 42 kDa chitinase in E. coli. The study demonstrated an economical method to produce chitinases for various agricultural and environmental applications.

Background: Patient satisfaction has played an important role in the development of health systems. However, the information on patient satisfaction and the returning of the patients for rehabilitation is limited. This study aims to explore the role of different domains in patient satisfaction with the willingness to return for further rehabilitation. Methods: The cross-sectional study was conducted among all 163 patients who were treated at different departments but needed a combination of treatment with rehabilitation at Viettiep hospital, Haiphong city. The Patient Satisfaction Index (PSI) Questionnaire was applied to record the average score of satisfaction. Multivariate logistic regression was used to determine the association between each domain of the PSI and the return for rehabilitation treatment. Results: Among the PSI scale, the strongest correlation was observed between the attitude of medical staff and the equipment and infrastructure of the hospital (r=0.305). The linear regression model indicated that patient satisfaction was associated significantly with the willingness to return for further rehabilitation (OR= 58.442). There were significant associations between the accessibility, equipment and infrastructure, quality of care and treatment, and cost of treatment with the returning for treatment of the patients. Conclusion: The patient satisfaction regarding individual domains should be considered in more detail in order to enhance the rehabilitation treatment of patients after discharging them from the hospital.

Vietnamese ginseng (Panax vietnamensis Ha et Grushv.), also known as Ngoc Linh ginseng, is a high-value herb in Vietnam. Vietnamese ginseng has been proven to be effective in enhancing the immune system, human memory, anti-stress, anti-inflammatory, anti-cancer, and prevent aging. The present study reports the first draft whole-genome of Vietnamese ginseng and the identification of potential genes involved in the triterpenoid metabolic pathway. De novo whole-genome assembly was performed successfully from a data of approximately 139 Gbps of 394,802,120 high quality reads to generate 9815 scaffolds with an N50 value of 572,722 bp from the leaf of Vietnamese ginseng. The assembled genome of Vietnamese ginseng is 3,001,967,204 bp long containing 79,374 gene models. Among them, there are 55,012 genes (69.30%) were annotated by various public molecular biology databases. The potential genes involved in triterpenoid saponin biosynthesis in Vietnamese ginseng and their metabolic pathway were also predicted.“ Three genes encoding squalene monooxygenase isozymes in Vietnamese ginseng were cloned, sequenced and characterized. Moreover, expression levels of several key genes involved in terpenoid biosynthesis in different parts of Vietnamese ginseng were also analyzed. The SSR markers were detected by various programs from both of assembly full dataset of Vietnamese ginseng genome and predicted genes. The present work provided important data of the draft whole-genome of Vietnamese ginseng for further studies to understand the role of genes involved in ginsenoside biosynthesis and their metabolic pathway at the molecular level of this rare medicinal species.

We examined gender differences among people who inject drug (PWID) in Hai Phong, Vietnam in term of blood-borne infections, risk behaviors, and access to care. Using respondent-driven-sampling surveys, we recruited 3146 PWID from 2016 to 2018. Inclusion criteria included a positive urine test for heroin and recent injection marks. There were 155 female PWID (4,9%), including 82 at RDS-2016, 32 at RDS-2017 and 38 at RDS-2018. The age mean was 36.3 ± 7.2 years. The majority of female PWID had less than high school education (90.9%) and were unemployed (51.3%). There was no difference in the proportion of HIV and HCV positive by gender. However, women had several significant differences in risk behaviors than men in multivariable logistic regression. Being a woman was independently associated with being unemployed, being a sex worker, having unstable housing, having uses drugs for less than 5 years, more use of methamphetamine, having a partner who ever injected drugs, and less access to methadone treatment. Interventions targeting female PWID are needed, possibly through community organizations and peer educators.

The present study reports the transient expression of chi42 genes encoding 42 kDa chitinase from T. asperellum SH16 in N. benthamiana via agroinfiltration. The efficacy of agroinfiltration for chi42 genes including a wild-type gene (Chi42) and two synthetic genes (syncodChi42-1 and syncodChi42-2) was determined. Accordingly, coinfiltration of two vectors pMYV719 carrying one of three genes chi42 and pMYV508 carrying gene p19 expedited the higher expression of recombinant enzymes whose genes were optimized for codon usage in plant tissues. The highest chitinolytic activity of about 290 U/mL was found in plants containing the gene syncodChi42-2 after 7 days of injection, 1.7 and 2.6 times higher than that of genes syncodChi42-1 and chi42. Recombinant chitinase has also exhibited activity against the pathogenic fungus Sclerotium rolfsii under in vitro condition. A higher expression level of syncodChi42-2 gene in N. benthamiana and its antifungal activity promise potential applications in the field of transgenic crops against phytopathogenic fungi. © 2021 Friends Science Publishers

Objectives: This study aims to evaluate the effectiveness of the reconstruction of tongue defects with submental flap after tumor resection. Methods: This is a retrospective and prospective study for 12 patients diagnosed with tongue cancer who underwent extensive tumor resection and reconstruction of tongue defects with submental flap at the Center for Craniofacial and Plastic Surgery - 108 Military Central Hospital, from January 2019 to March 2022. Results: The study included 10 male patients (83.3%) and 2 female patients (16.7%), with an average age of 54.83. The average size of the defect is 54.4x 30.2mm. The submental flap for reconstruction had an average size of 69.6x39.6mm. After surgery, the survival rate of the flap was 100%, partial necrosis was 7.1%, damage to the mandibular branch was 7.1%. Resumption of completely normal speech function accounted for 66.7%, normal diet accounted for 58.4%, no patient had completely lost speech or swallowing function. Conclusion: The submental flap has many advantages in reconstructing tongue defects after small-size tumor resection, including simple technique and suitable size. Reconstructive results show the good restoration of the patients' speech and swallowing functions.

Objective: To evaluate the treatment results of mandibular condylar fractures for prescription of more appropriate treatment methods. Methods: This is a retrospective and prospective cross-sectional study with follow-up on 95 patients treated for mandibular condylar fractures at 108 Central Military Hospital from January 2018 to October 2021. Results: A total of 95 condylar fractures were observed, including 75 unilateral fractures and 20 bilateral fractures. The most common fracture locations are: low condylar neck 53/115 (46.1%), condylar head 27/115 (23.5%), high condylar neck 22/115 (19.1%), subcondylar 13/115 (11.3%). Little deviation 13/115 (11.3), average deviation 79/115 (68.7%), high deviation 23/115 (20%). Conservative treatment for 18/115 condyle with good and satisfactory results accounted for 89.3%. Surgical removal of 33/115 condyles with good and satisfactory results accounted for 89.3%. Fracture osteosynthesis for 64/115 condyles with good and satisfactory results accounted for 96.2%. Conclusion: Conservative treatment should be indicated for cases with small or no displacement and condylar removal surgery for cases of condylar fractures or multi-fragment high condylar fractures with moderate and high displacement. The indication of osteosynthesis for low condylar neck and subcondylar fractures will have a high success rate.

Tofu is a common food in Vietnamese and global cuisine. It has high nutritional value, good flavor and texture that it is easy to produce other food from it. In Hanoi, Mo tofu is a traditional product that produced from tofu- whey, which is commonly produced by small scale producers. In this research, effect of coagulating pH on the formation of some linkages and tofu texture and qualities. The results showed that pH effected on S-S linkage and Ho index or phobicity on Mo tofu. At pH=5.8, Mo tofu had the highest moisture of 83.71%, highest water holding capacity of 8.96g/g protein, low hardness, rather high elasticity

Facial nerve palsy is one of the most common complications in parotid gland surgery. This report evaluates the effectiveness of applying Tumescent solution for preserving the facial nerve in parotidectomy.Prospective and descriptive clinical study on 34 patients undergoing parotidectomy with facial nerve preservation. Before skin incision, 5-10 min, we injected 100-200 ml of the Tumescent solution into the surgical area. We recorded the surgical method, tumor size, length of surgery, pathological results and facial nerve dysfunction. All patients were followed for a period ranging from 6 to 24 months.There were 14 patients with malignant tumors and 20 patients with benign tumors. The length of surgery lasted from 90 to 180 min, with an average of 126.8 min. The number of patients having temporary facial paralysis was 22 cases (64.7%), recovery time ranged from 1 week to 6 months, and no permanent facial paralysis cases were recorded. The clinical occurrence of Frey's syndrome was five cases (14.7%).The application of Tumescent solution for preserving facial nerves in parotidectomy could minimize nerve injury and shorten the length of surgery.

An adenomatoid odontogenic tumor is a rare medical condition. Large tumor (or several) often appears in the maxillae. In a minority of cases, the tumor(s) appear in the mandible.We report on a case of a 24-year-old female diagnosed with a mandibular adenomatoid odontogenic tumor, a giant tumor measuring approximately 22 × 25 × 17 cm. The tumor was located on the side of the mandible, causing facial deformity, malnutrition, and hemorrhaging. We assessed the patient's overall condition, carried out a resection of the tumor and mandible from the right condyle to the left mandibular angle, and reconstructed the mandibular defect with a fibula free flap. After the treatment, the patient was followed up for 1 year, with no recurrence detected over this period.Because adenomatoid odontogenic tumors are benign odontogenic lesions, which are painless and slow-growing, most are surgically removed or treated conservatively. However, the above treatment measures cannot be applied in the case of a giant tumor that causes facial deformity, destroys the entire jawbone, and has complications such as hemorrhaging and malnutrition. After the tumor resection, the defect is still significant. Accordingly, reconstruction using a microsurgical bone flap is an effective method instead.Large adenomatoid odontogenic tumors in the mandible are rare, and treatment cannot follow conventional methods. Accordingly, defect reconstruction after tumor resection is essential.

The goal of this study was to investigate the anatomy of the perforators from the peroneal artery in Vietnamese patients.30 cadaver's legs were dissected and investigated for the distribution, course, origin, number and types of perforators of the peroneal artery. The locations of the exit points on the skin of perforators were marked in relation to reference points and segments.The total number of cutaneous perforating branches of the peroneal artery from 30 specimens was 149, which included 63 (42.2%) musculocutaneous perforators and 86 (57.8%) septocutaneous perforators. In most cases, the perforator branches were located in the range from 4 to 7 of the total fibula length (69.8%). The average number of perforating vessels in a leg was 4.9, ranging from 1 to 8 vessels. All the perforators were positioned behind the posterior border of the peroneal bone. In all the dissected samples presented, there was always one cutaneous perforator within a distance of 18 mm from the F point, which is the junction between the 6/10 and 7/10 segments at the posterior border of the fibular bone.The abundance of cutaneous perforators in Vietnamese patients can be used to plan various combined skin and bone flaps. A cutaneous perforator was consistently found near the F point, and this factor can be used in the planning of a bone flap with accompanying skin for monitoring survival of the underlying fibular bone flap.

In this study, morphogenesis and anatomy of adventitious roots and hairy roots of Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) were compared. Adventitious roots were derived from four different samples (leaf, petiole, root and callus) in vitro cultured on medium SH supplemented with 5 mg/L IBA. Hairy roots were derived from callus infected with Agrobacterium rhizogenes strain ATCC 15834. The results showed that there were significant differences in morphogenesis and anatomy, the pathway of secondary roots formation, culture medium between adventitous roots and hairy roots. The hairy roots strongly grew and branched on the free-plant growth regulator medium. The secondary root of the hairy root was formed in two pathways: (1) The secondary root formation began at the vesicles system of main root; (2) The secondary root formation was independent of the vesicles system of main root; it kept developing in length and connected to the vesicles system of main root. On the other hand, growing adventitous roots only grew on the environment with exogenous auxin supplementation; also their branching ability were low. The secondary root formation of the adventitous root was started at the vesicles of the main roots and there was only one secondary root that was formed at an location of adventitous root. The results that obtained in this study will be the reference to identify two types of root.

This paper presents the long-term morphological changes of the sand spits at the Ken Inlet in Ha Tinh Province and Phan Inlet in Binh Thuan Province, Vietnam. The analysis results show that the sand spit morphology at Ken Inlet was drastically changed before the completion of the Da Bac sluice gate construction in 1992, after that the sand spit elongation rate became stable at a rate of about 68 meters per year. Meanwhile, the sand spit at Phan Inlet was breached three times during the winter months of 1990-1991, 1998-1999 and 2014-2015. Moreover, the results of remote sensing image analysis also show that after the sand spit have been breached, it continued elongating at a relatively stable rate of 170÷200 meters per year. Based on the analytical model by Kraus (1999) for predicting the sand spit elongation, the estimated long-shore sediment transport rates of Phan Inlet and Ken Inlet are 145,000 m3/year and 133,500 m3/year, respectively. These longshore sediment transport rates are a main contribution for the sand spit elongation in these study areas. Keywords: sand spits; tidal Inlet; breaching; elongation; Landsat images; Google Earth images.

Background: Fibrinolytic enzymes, such as Nattokinases from Bacillus species are known to degrade the fibrin blood clots. They belong to serine protease group having commercial applications, such as therapeutic agents and functional food formulation. Objective: The present study reports some characteristics and fibrinolytic activity of serine protease from B. subtilis C10 strain that was isolated from shrimp shell. Method: Extracellular enzyme from B. subtilis C10 culture was harvested and partially purified by ammonium sulphate precipitation. Fibrinolytic activity of the enzyme was determined by zymography and measured by spectrophotometry with fibrinogen and thrombin used as substrates. The optimal temperature and pH for fibrinolytic activity were studied in the range of 31-43ºC and 5-10, respectively. The thermal and pH stability of enzyme was studied by incubating enzyme for 30 min in the same range of temperature and pH as above. The effect of some metal ions and reagents on fibrinolytic activity of enzyme was evaluated by concentrations of 5 mM and 5%, respectively. Results: Zymogram analysis indicated the presence of four fibrinolytic enzymes with molecular weights of approximately 69, 67, 39 and 36 kDa. The optimal temperature and pH for enzyme activity were 37°C and 9, respectively. The thermal and pH stability ranged from 35-39°C and 8-10, respectively. Fibrinolytic activity reached a maximum value of about 400 U/mg protein after 16 h of C10 strain culture. Enzyme has been drastically inhibited by PMSF and SDS, and partially inhibited by EDTA, while Triton X-100 has significantly increased enzyme activity. Effects of ions such as Mg2+, Ca2+ and Mn2+ on enzyme were negligible, except Cu2+ and Zn2+ have strongly decreased its activity. Conclusion: Results from the present study suggested that enzyme obtained from B. subtilis C10 could be serine protease that has a high fibrinolytic activity up to about 400 U/mg protein at the most appropriate temperature and pH of 37ºC and 9. This activity can be improved up to 142% by incubating enzyme with 5% Triton X-100 for 30 min.

This study reports the expression of 42 kDa chitinase genes from Trichoderma asperellum SH16 in peanut (Arachis hypogaea) roots under the regulation of tissue-specific Asy promoter through Agrobacterium tumefaciens-mediated transformation. The 42 kDa chitinase genes, including one wild-type sequence (Chi42) and two synthetic sequences (syncodChi42-1 and syncodChi42-2) which were optimized for codon usage for plant expression, were incorporated into the peanut genome and successfully expressed in their roots. The investigation revealed that the enzyme chitinase from two synthetic genes had higher activity than that from the wild-type gene, about 901 U/mg (140 U/mL) and 1124 U/mg (197 U/mL) vs about 508 U/mg (87 U/mL). Transgenic peanut roots also exhibited extracellular chitinase activity which was driven by signal peptide of rice amylase 3D gene against the pathogenic fungus Sclerotium rolfsii under in vitro conditions. The higher chitinase activity of two synthetic genes in peanut roots promises potential applications in the field of transgenic crops against phytopathogenic fungi.

Eriodictyol is a flavonoid in the flavanones subclass. It is abundantly present in a wide range of medicinal plants, citrus fruits, and vegetables. In addition, eriodictyol owns numerous importantly medicinal bioactivities such as inhibition of proliferation, metastasis and induction of apoptosis in glioma cells or inhibition of glioblastoma migration, and invasion. This study described the heterologous production of eriodictyol by E. coli based co-culture engineering system from the initial substrate as D-glucose. Notably, the upstream module was composed of genes for synthesis of p-coumaric acid (pCA) from D-glucose. The downstream module consisted of genes for the synthesis of eriodictyol from p-coumaric acid. The maximal result of eryodictyol was achieved 51.5 mg/L using optimal culture conditions, while mono-culture was only achieved 21.3 mg/L. In conclusion, co-culture was the efficiently alternative approach for the synthesis of eriodictyol and other natural products.

Background: Hyaluronic acid (HA) fillers for aesthetic are generally considered safe and the use of dermal fillers for soft tissue augmentation has become a very popular technique in aesthetic practices. Dermal fillers temporarily remove the appearance of rhytids and reduce the depth of skin folds. This is one of the most effective method for facial improvement used in Viet Nam nowadays. However, complications such as skin necrosis, blind... may happen and hence any filler injection practitioner need to be aware of such side effects, contraindications and precaution to be adopted while using fillers. Even with the most experienced of injectors, adverse effects can and do occur ranging from mild bruising to severe injection necrosis. Aims: HA filler injectors should be able to prevent and treat the severe complication of skin necrosis and detect impending necrosis after injection of a augmentation filler. Materials and Methods: Cases report of 3 patients who were HA filler injection for nose augmentation and had suffered from skin necrosis. These patients were followed for 3-6 months from time of injection of hyaluronic acid filler to complete healing of wound. Results: Complete wound healing were achieved with early recognition and institution of treatment, surgical procedures applied in some severe cases. The functional of the noses are remained but most of the patients have bad scar on the nose. Discussion: We review cases report of injection necrosis and methods used to prevent and treat this complication. Conclusion: Early recognition of vascular necrosis with specific protocol for treatment after injection necrosis with hyaluronic acid fillers improves the outcome of wound healing.

Buttock soft tissue defects can be encountered due to many causes and also have many treatment solutions. Usually, defects are common on one side of the buttocks and cutaneous skin flaps in place, local or regional used. The article analyzes a rather rare case with a soft tissue defect in the buttocks on both sides as a necrotic sequela after injecting fillers for cosmetic purposes. After treatment of necrotizing inflammation, the sequelae left behind is a concave scar and deformed buttock on both sides. The local fat flaps are not valuable in this case, while the free fat flap will face many risks of failure due to the use of 2 flaps on both sides, the post-operative care position is also very difficult, easy to cause tension, compress the vascular pedicles. This clinical case with the use of bilateral DIEP flaps, and the postoperative results in this case are analyzed and evaluated in the article.

Vietnamese ginseng (Panax vietnamensis Ha et Grushv.), also known as Ngoc Linh ginseng, is a high-value herb in Vietnam. Vietnamese ginseng has been proven to be effective in enhancing the immune system, human memory, anti-stress, anti-inflammatory, anti-cancer, and prevent aging. The present study reports the first draft whole-genome of Vietnamese ginseng and the identification of potential genes involved in the triterpenoid metabolic pathway. De novo whole-genome assembly was performed successfully from a data of approximately 139 Gbps of 394,802,120 high quality reads to generate 9815 scaffolds with an N50 value of 572,722 bp from the leaf of Vietnamese ginseng. The assembled genome of Vietnamese ginseng is 3,001,967,204 bp long containing 79,374 gene models. Among them, there are 55,012 genes (69.30%) were annotated by various public molecular biology databases. The potential genes involved in triterpenoid saponin biosynthesis in Vietnamese ginseng and their metabolic pathway were also predicted.“ Three genes encoding squalene monooxygenase isozymes in Vietnamese ginseng were cloned, sequenced and characterized. Moreover, expression levels of several key genes involved in terpenoid biosynthesis in different parts of Vietnamese ginseng were also analyzed. The SSR markers were detected by various programs from both of assembly full dataset of Vietnamese ginseng genome and predicted genes. The present work provided important data of the draft whole-genome of Vietnamese ginseng for further studies to understand the role of genes involved in ginsenoside biosynthesis and their metabolic pathway at the molecular level of this rare medicinal species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-021-01076-1.

Chitinases are enzymes that catalyze the degradation of chitin, a major component of the cell walls of pathogenic fungi and cuticles of insects, gaining increasing attention for the control of fungal pathogens and insect pests. Production of recombinant chitinase in a suitable host can result in a more pure product with less processing time and a significantly larger yield than that produced by native microorganisms. The present study aimed to express the synthetic chi42 gene (syncodChi42), which was optimized from the chi42 gene of Trichoderma asperellum SH16, in Escherichia coli to produce 42 kDa chitinase (Ta-CHI42); then determined the activity of this enzyme, characterizations and in vitro antifungal activity as well as its immunogenicity in mice. The results showed that Ta-CHI42 was overexpressed in E. coli. Analysis of the colloidal chitin hydrolytic activity of purified Ta-CHI42 on an agar plate revealed that this enzyme was in a highly active form. This is a neutral chitinase with pH stability in a range of 6–8 and has an optimum temperature of 45°C with thermal stability in a range of 25–35°C. The chitinolytic activity of Ta-CHI42 was almost completely abolished by 5 mM Zn2+ or 1% SDS, whereas it remained about haft under the effect of 1 M urea, 1% Triton X-100 or 5 mM Cu2+. Except for ions such as Mn2+ and Ca2+ at 5 mM that have enhanced chitinolytic activity; 5 mM of Na+, Fe2+ or Mg2+ ions or 1 mM EDTA negatively impacted the enzyme. Ta-CHI42 at 60 U/mL concentration strongly inhibited the growth of the pathogenic fungus Aspergillus niger. Analysis of western blot indicated that the polyclonal antibody against Ta-CHI42 was greatly produced in mice. It can be used to analyze the expression of the syncodChi42 gene in transgenic plants, through immunoblotting assays, for resistance to pathogenic fungi.

The porcine epidemic diarrhea virus (PEDV) belongs to the coronavirus family, which causes acute diarrhea in pigs with higher mortality in piglets less than 2 weeks old. The PEDV is one of the major concerns of the pig industry around the world, including Asian countries and Noth America since first identified in Europe. Currently, there is no PEDV licensed vaccine to effectively prevent this disease. This study was performed for the development of a mucosal PEDV vaccine and B subunit of cholera toxin (CTB) as a carrier was employed to surpass the tolerogenic nature of GALT and induce potent immune responses against the target antigen fused to CTB. An epitope (S1D) alone or conjugated with CTB was constructed into the tobacco chloroplasts expression vector which is controlled under the chloroplast rRNA operon promoter with T7g10 5′ UTR and the psbA 3′UTR as a terminator. The homoplastomic lines were obtained by third round screening via organogenesis from the leaf tissues which were verified by PCR with antigen and chloroplast specific primers and then confirmed by Southern blot analysis. While the expression level of the S1D alone as detected by Western blotting was approximately 0.07% of total soluble protein, the CTB-S1D fusion protein was expressed up to 1.4%. The fusion protein showed binding to the intestinal membrane GM1-ganglioside receptor, demonstrating its functionality. The result shows that the highest expression of S1D could be achieved by fusion with a stable CTB protein and chloroplast transformation. Furthermore, the CTB-S1D expressed in chloroplasts of Nicotiana tabacum cv. Maryland could be assembled to pentameric form which increases the possibility to develop a mucosal vaccine against PEDV., Key message This study describes a protocol for improved plant expression of a porcine epidemic diarrhea immunogen by utilisation of a CTB fusion protein and chloroplast transformation. The results show that the production of CTB fusion antigen was twenty times higher than antigen alone in transplastomic plant.

Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana be nthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.

The issue of the relationship between private property and common property has engaged both legal and economic scholars in a long series of controversies over the meaning, the sequence of development, and the superiority of private vs. common property. The issues debated relate to the efficiency, equity and sustainability of private property as contrasted to common property. Many scholars think of contemporary examples of common property as remnants of the past, likely to disappear during the twenty-first century. Recent research, however, has challenged the presumption that private property is necessarily superior to common property. Keywords: Private property, common property, open access regimes. References: [1] Grossi, Paolo (1981), An Alternative to Private Property; Collective Property in the Juridical Consciousness of the Nineteenth Century, Chicago: University of Chicago Press.[2] Maine, Henry Sumner (1963), Ancient Law: Its Connection with the Early History of Society and its Relation to Modern Ideas; With Introduction and Notes by Frederick Pollack, Boston: Beacon Press (reprint of 1861 ed.), p. 252.[3] North, Douglass C. and Robert Paul Thomas (1976), The Rise of the Western World: A New Economic History, London: Cambridge University Press.[4] North, C. Douglass, L. Terry Anderson, and J. Peter Hill (1983), Growth and Welfare in the American Past: A New Economic History, Englewood Cliffs, NJ: Prentice-Hall.[5] W.P. Welch, “The political feasibility of full ownership property rights: The cases of pollution and fisheries”, Policy Sciences, (1983), 16, 165-80.[6] Ostrom, Vincent, The Intellectual Crisis in American Public Administration, 3rd ed., Tuscaloosa: University of Alabama Press (2008).[7] North, C. Douglass, Institutions, Institutional Change, and Economic Performance, New York: Cambridge University Press (1990).[8] Elinor Ostrom and Charlotte Hess, Private and Common Property Rights, Workshop in Political Theory and Policy Analysis, Indiana University, 29/11/2007, https://surface.syr.edu/cgi/viewcontent.cgi?article=1024&context=sul (truy cập lần cuối: 31/03/2019).[9] Ciriacy-Wantrup, V. Siegfried and C. Richard. Bishop (1975), “Common property” as a concept in natural resource policy, Natural Resources Journal, 15, 713-727.[10] Báo Pháp luật (điện tử): https://plo.vn/thoi-su/dai-bieu-lo-dan-het-duoc-tu-do-tam-bien-o-phu-quoc-774398.html (truy cập lần cuối: 05/04/2019).[11] Báo Tuổi Trẻ (điện tử): https://tuoitre.vn/khong-the-chap-nhan-tinh-trang-lay-bien-lam-cua-rieng-2018110711421466.htm (truy cập lần cuối: 05/04/2019.[12] Dales, H. John (1968), Pollution, Property, and Prices: An Essay in Policy-Making and Economics, Toronto: University of Toronto Press.[13] Higgs, Robert (1996), “Legally induced technical regress in the Washington salmon fishery”, in L. J. Alston, Thráinn Eggertsson, and Douglass North (eds), Empirical Studies in Institutional Change, New York: Cambridge University Press.[14] Johnson, N. Ronald and D. Gary Libecap (1982), “Contracting problems and regulation: The case of the fishery”, American Economic Review, 72, 1005-1022.[15] Wiersma, L. Lindsey (2005), “Indigenous lands as cultural property: A new approach to indigenous land claims”, Duke Law Journal, 54, 1061-1088.[16] Ostrom, Vincent and Elinor Ostrom (1977), “A theory for institutional analysis of common pool problems”, in Garrett Hardin and John Baden (eds), Managing the Commons, San Francisco, CA: W. H. Freeman, pp. 157-172.[17] Ostrom, Elinor, Roy Gardner, and James M. Walker (1994), Rules, Games, and Common-Pool Resources, Ann Arbor: University of Michigan Press.[18] Feeny, David, Fikret Berkes, Bonnie J. McCay, and James M. Acheson (1990), “The tragedy of the commons: Twenty-two years later”, Human Ecology, 18, 1-19.[19] Bromley, Daniel W. (1992), “The commons, common property, and environmental policy”, 2 Environmental and Resource Economics, 1-17.[20] Singh, Katar (1994), Managing Common Pool Resources: Principles and Case Studies, New Delhi: Oxford University Press. [21] Singh, Katar and Vishwa Ballabh (1996), Cooperative Management of Natural Resources, New Delhi: Sage.[22] Blomquist, William and Elinor Ostrom (1985), “Institutional capacity and the resolution of a commons dilemma”, Policy Studies Review, 5, 383-393.[23] Lueck, Dean (1995), “The rule of first possession and the design of the law”, Journal of Law and Economics, 38, 393-436.[24] Ghoshal, Sumantra and Peter Moran (1996), “Bad for practice: A critique of the transaction cost theory”, Academy of Management Review, 21, 13-47.[25] Putterman, Louis (1995), Markets, hierarchies, and information: On a paradox in the economics of organization”, Journal of Economic Behavior and Organization, 26, 373-390.[26] Seabright, Paul (1993), “Managing local commons: Theoretical issues in incentive design”, Journal of Economic Perspectives, 7, 113-134.

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.

Porcine epidemic diarrhea virus (PEDV) belongs to the Coronaviridae family and causes acute enteritis in pigs. A fragment of the large spike glycoprotein, termed the S1D epitope (aa 636–789), alone and fused with cholera toxin B subunit, were independently cloned into plant expression vectors, yielding plasmids pMYV717 and pMYV719, respectively. Plant expression vectors were transformed into Agrobacterium tumefaciens and subsequently infiltrated into Nicotiana benthamiana leaves. The highest expression level of S1D was found at 2 days post infiltration (dpi), reached 0.04 % of total soluble protein, and rapidly decreased thereafter. The expression and assembly of CTB–S1D fusion protein were confirmed by Western blot and GM1-ELISA. The highest expression level of CTB–S1D fusion protein was 0.07 % of TSP at 4 dpi, with a rapid decrease thereafter. In the presence of p19 protein from tomato bushy stunt virus, the S1D and CTB–S1D protein levels peaked at 6 dpi and were fourfold to sevenfold higher than in the absence of p19, respectively. After oral administration of transiently expressed CTB–S1D fusion protein, or with bacterial cholera toxin or rice callus expressing mutant cholera toxin 61F, mice exhibited significantly greater serum IgG and sIgA levels against bacterial CTB and S1D antigen, peaking at week 6. Transiently expressed CTB–S1D fusion protein will be administered orally to pigs to assess the immune response against PEDV.

Periodontitis is a bacterial infection-induced inflammatory disease that affects the tissues surrounding the teeth and is the main cause of tooth loss in adults. Porphyromonas gingivalis, a black-pigmented gram-negative anaerobe, is a major causative agent of periodontitis. A 53-kDa protein of the bacterium has been demonstrated to be highly immunogenic and recognized by sera from most periodontitis patients, and it is now considered as a potential target of protective immunity. In this study, complementary DNA encoding monoclonal antibodies to 53-kDa protein of P. gingivalis A7A1-28 was cloned from hybridoma cell lines expressing the antibodies. The heavy and light chain genes were constructed into plant expression vectors under the control of the rice α-amylase 3D (RAmy3D) promoter. The recombinant plant expression vectors were introduced into rice cells (Oryza sativa L. cv. Dongjin) using particle bombardment transformation method. Transgenic rice calli were selected by genomic DNA PCR and used to establish cell suspension cultures. The expression of the heavy and light chain genes of the monoclonal antibodies in the rice cell suspension cultures was detected by western blot analysis. The plant-produced monoclonal antibodies showed specific reactivity with 53-kDa proteins of P. gingivalis. These results suggest that 53-kDa protein-specific monoclonal antibodies produced in the rice cell suspension cultures are biologically active against the bacterial protein and can be used for passive immunization to prevent P. gingivalis-induced periodontal disease.

Tidal inlets along the central coast of Vietnam are located in a microtidal, wave-dominated coastal environment. In addition, the Vietnam coast is highly influenced by the seasonal monsoon regime, which is characterized by large northeast waves from October to March and calm southeast waves from April to September every year. Consequently, the tidal inlet entrance morphologies often suffer from a dynamic seasonal evolution due to distinct differences in the direction of wave-induced longshore sediment transport (LST) between the two monsoon seasons. The migration or closure of tidal inlets causes a lot of problems for socio-economic development in the region since these are the main reasons leading to an increase in the risk of coastal flooding and the obstruction of navigation. This paper presents a comprehensive study of the morphological evolutions of natural tidal inlets on the central coast of Vietnam using long-term remote sensing data sets and by the Delft3D numerical model. Surprisingly, the estimated LST rates from the former method are in an order of magnitude agreement with the results from the latter one for all of the areas in this study. Based on the conservation equation for sand and comprehensive data collection, a new simple empirical formula for predicting the sand spit elongation rate as a function of the sand spit width is developed. Although the breaching of sand spit might happen during an extreme flood event at some tidal inlets, the growth rate of the spit before and after the breaching is almost unchanged. These findings are very useful information for supporting the local coastal authorities to find better management solutions in terms of sustainable development.

Elongation of the sand spit in Loc An River Mouth located in the Southern Coast of Vietnam was studied using a series of satellite images to overcome the problem associated with lacking data in developing countries like Vietnam. Characteristic changes of the sand spit have been figured out based on the waterlines extracted from the re-rectified satellite images. In addition, longshore sediment transport rate (LSTR) along the up-drift sand spit was calculated based on the idea of the one-line model and this value shows good agreement with the numerical calculation performed in another study of this area.

The rates of mosquito-transmitted dengue virus infection in humans have increased in tropical and sub-tropical areas. Domain III of dengue envelope protein (EDIII) is involved in cellular receptor binding and induces serotype-specific neutralizing antibodies. EDIII fused to the B subunit of Vibrio cholera (CTB-EDIII) was expressed in potatoes to develop a plant-based vaccine against dengue virus type 2. CTB-EDIII fused to an endoplasmic reticulum (ER) retention signal, SEKDEL, was introduced into potatoes by A. tumefaciens-mediated gene transformation. The integration of the CTB-EDIII fusion gene into the nuclear genome of transgenic plants was confirmed by genomic DNA polymerase chain reaction (PCR), and mRNA transcripts of CTB-EDIII were detected. CTB-EDIII fusion protein was expressed in potato tubers and assembled into a pentameric form capable of binding monosialotetrahexosylganglioside (GM1). The level of expression was determined to be ∼0.005% of total soluble protein in potato tubers. These results suggest that dengue virus antigen could be produced in potatoes, raising the possibility that edible plants are employed in mucosal vaccines for protection against dengue infection.

Duy, D.V.; Tanaka, H.; Mitobe, Y.; Anh, N.Q.D., and Viet, N.T., 2018. Sand spit elongation and sediment balance at Cua Lo Inlet in Central Vietnam. In: Almar, R.; Almeida, L.P.; Trung Viet, N., and Sall, M. (eds.), Tropical Coastal and Estuarine Dynamics. Journal of Coastal Research, Special Issue No. 81, pp. 32–39. Coconut Creek (Florida), ISSN 0749-0208.Erosion is significant on the downdrift beach of Cua Lo Inlet, which is located in Central Vietnam. The elongation of Cua Lo Inlet's updrift sand spit together with severe erosion of the downdrift bank is raising numerous social problems such as flood safety and land loss. In this study, a series of Landsat and Google Earth images were used to analyze and investigate the inlet recent morphological changes and evolution. Since 1970 the inlet has constantly migrated towards the sediment transport pathway, and its traveling distance has reached 1700 m. In order to quantitatively investigate such characteristics, the tip coordinates and the area of the sand spit updrift of the inlet were calculated. In particular, the area of the sand spit has increased remarkably, and the increasing rate is almost constant. Using this value, the rate of sand volume transported along the updrift sand spit by the waves was estimated and it is in good agreement with the longshore sediment transport rate of the surrounding beach.

A gene encoding the B subunit of the enterotoxigenic Escherichia coli heat-labile enterotoxin (LTB) was adapted to the optimized plant coding sequence, and fused to the endoplasmic reticulum retention signal SEKDEL in order to enhance its expression level and protein assembly in plants. The synthetic LTB (sLTB) gene was placed into a plant expression vector under the control of the CaMV 35S promoter, and subsequently introduced into the watercress (Nasturtium officinale L.) plant by the Agrobacterium-mediated transformation method. The integration of the sLTB gene into the genomic DNA of transgenic plants was confirmed by genomic DNA PCR amplification. The assembly of plant-produced LTB protein was detected by western blot analysis. The highest amount of LTB protein produced in transgenic watercress leaf tissue was approximately 1.3% of the total soluble plant protein. GM1-ganglioside enzyme-linked immunosorbent assay indicated that plant-synthesized LTB protein bound specifically to GM1-ganglioside, which is the receptor for biologically active LTB on the cell surface, suggesting that the plant-synthesized LTB subunits formed biologically active pentamers.

By generalizing a known result for double-error-correcting binary primitive Bose\3-Chaudhuri\3-Hocquenghem codes, the following statement is proved: A necessary condition for L-step orthogonalization of a t-error-correcting binary linear code C of length n is given by n ≥ {if123-1} + {if123-2} if its expurgated version C′ is distinct from C, and n ≥ {if123-3} if C′ is identical to C, where {if123-4} is the minimum distance of the dual {if123-5} of the code C′, and {if123-6} is that of the dual {if123-7} of the code C′, expurgated from C by a single information digit. From this result, it is found that the following codes cannot be L-step orthogonalized: triple-error-correcting BCH codes with odd m greater than 5 and even m equal to 8 and 10, all the BCH codes with m = 7 except two trivial cases, and most of the binary quadratic residue codes with known minimum Hamming distances. In addition, various codes are analyzed; and where possible, the types of majority-logic decoding are tabulated for these codes, namely, a class of binary cyclic codes of length n ≤ 43 and all the binary BCH codes of length n = 63.