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A streamlined approach toward the rapid fabrication of streptavidin-biotin-based protein microarrays was investigated. First, using our engineered versatile plasmid (pBADcM-tBirA) and an optimal coexpression strategy for biotin ligase and biotin acceptor peptide (BAP) chimeric recombinant protein, an autogeneration system for biotinylated probes was developed. This system permitted an advantageous biotinylation of BAP chimeric recombinant proteins, providing a strategy for the high-throughput synthesis of biotinylated probes. Then, to bypass the conventional rate-limiting steps, we employed an on-chip purification process to immobilize the biotinylated probes with high-throughput recombinant lysates. The integration of the autogeneration of probes and on-chip purification not only contributed to the effective and reliable fabrication of the protein microarray, but also enabled simplification of the process and an automated throughput format. This labor- and cost-effective approach may facilitate the use of protein microarrays for diagnosis, pharmacology, proteomics, and other laboratory initiatives.

This paper presents a novel three-coil reconnection electromagnetic launcher (TCREL). Analytical calculations and simulation results indicated that not only does this model possess a higher structural strength, but also the launching efficiency is improved effectively compared with that of the traditional reconnection electromagnetic launcher (REL). In addition, take the TCREL model as an independent module; a toroidal REL (TREL) model can be obtained after several modules are uniformly placed and fixed around the circumference. The TREL model can reduce the magnetic flux leakage among driving coils, and further improves the launching efficiency. To verify the conceptual feasibility, two-stage TCREL and REL models were built and tested. The experiments demonstrate that the TCREL model possesses better launching performance than does the REL model; the former can obtain the desired launching efficiency of 15.818% but the latter only 8.248% in the contrast experiment. The experimental results are also in accordance with the simulation results.