1. High-fat diet-induced obesity amplifies HSP70-2a and HSP90 expression in testicular tissue; correlation with proliferating cell nuclear antigen (PCNA)
- Author
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Hamed Rezaei Agdam, Sana Moshari, Masoumeh Moradi-Ozarlou, Mazdak Razi, Simineh Shahmohamadlou, and Amir Nomanzadeh
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,DNA repair ,DNA damage ,Diet, High-Fat ,030226 pharmacology & pharmacy ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Heat shock protein ,Internal medicine ,Proliferating Cell Nuclear Antigen ,Testis ,medicine ,Animals ,HSP70 Heat-Shock Proteins ,HSP90 Heat-Shock Proteins ,Obesity ,General Pharmacology, Toxicology and Pharmaceutics ,Rats, Wistar ,Testosterone ,biology ,Leydig cell ,General Medicine ,Proliferating cell nuclear antigen ,Hsp70 ,Rats ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,biology.protein ,Immunohistochemistry - Abstract
Aims Current study was conducted to uncover the effect of high-fat diet (HFD)-induced obesity on heat shock proteins 70-2a and 90 expression levels and to investigate the network between these proteins with PCNA expression, endocrine status of testicular tissue and nucleotide backbone damages. Main methods For this purpose, 20 mature male Wistar rats were divided into two groups of control and HFD-received obese animals (n = 10/group). After 8 weeks from obesity approval, the animals were euthanized. The expression levels of Hsp70-2a, Hsp90 and PCNA were analyzed by qRT-PCR and immunohistochemical staining techniques. The Leydig cell distribution/mm2 of interstitial tissue, serum level of testosterone, testicular total antioxidant capacity (TAC), and mRNA and DNA damage were investigated. Key findings The obese (HFD-received) animals represented a remarkable (p Significance Therefore, considering massive testicular DNA damage in the obese (HFD-received) animals, we can conclude that an increased expression of Hsp70-2a and Hsp90 with no harmony with PCNA could not properly maintain the cellular DNA integrity and/or appropriately finalize the DNA repair process.
- Published
- 2021