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We present a general lower bound for the fundamental tone for the p-Laplacian on Riemannian manifolds carrying a special kind of function. We then apply our result to the cases of negatively curved simply connected manifolds, a class of warped product manifolds and for a class of Riemannian submersions.

High case counts after the Gamma (P. 1) variant of severe acute respiratory syndrome coronavirus 2 emerged in Brazil raised concerns that previously infected persons might become reinfected. Investigation of a cluster of coronavirus disease cases in Parintins, in the Brazilian Amazon, suggested household transmission but did not identify high rates of reinfection.

The authors describe, discuss, and illustrate two new species of Tillandsia from a granitic outcrop in the municipality of Petrópolis, Rio de Janeiro state, and from an inselberg located in the municipality of Belo Jardim, Pernambuco state, Brazil. The species are endemic with a restricted area of distribution, being considered critically endangered (CR) mainly due to habitat fragmentation and illegal extractivism, specifically for regional and international trade. We also present the phenology, a distribution map of the species, and compare them with morphologically similar taxa.

ARMC5 is a tumor suppressor gene frequently mutated in primary bilateral macronodular adrenal hyperplasia (PBMAH), an adrenal cause of Cushing’s syndrome. The function of ARMC5 is poorly understood, aside the fact that it regulates cell viability and adrenal steroidogenesis by mechanisms still unknown. Tumor suppressor genes play an important role in modifying intracellular redox response, which in turn regulates diverse cell signaling pathways. In this study we demonstrated that ARMC5 inactivation in adrenocortical cell increased expression of actors scavenging ROS, such as superoxide dismutases (SOD) and peroxiredoxins (PRDX) by increasing the transcriptional regulator NRF1. Moreover, ARMC5 is involved in the NRF1 ubiquitination and in its half-life. Finally, ARMC5 inactivation alters adrenocortical steroidogenesis through activation of p38 pathway and decreases cell sensitivity to ferroptosis participating to increase cell viability. Altogether, this study uncovers a function of ARMC5 as a regulator of the redox homeostasis in adrenocortical cells, controlling steroidogenesis and cell survival.

O estilo de vida da sociedade vem mudando cada vez mais, devido a busca por uma alimentação mais prática, aumentando o índice de produtos alimentícios processados. Tais alimentos possuem alto teor de açúcar, sódio, gordura e colesterol, responsáveis pelo desenvolvimento de doenças que prejudicam a saúde. Devido à má alimentação, observou-se a necessidade de alimentos que propiciassem a melhor qualidade de vida, portanto criou-se os produtos diet e light, que possuem em sua formulação a retirada ou diminuição de algum componente nutricional. O objetivo deste trabalho foi avaliar a rotulagem de alimentos diet e light comercializados na cidade de Currais Novos no Rio Grande do Norte, verificando-se os rótulos, conforme a legislação, para as informações obrigatórias e nutricionais. Foram avaliados 12 rótulos de marcas diferentes de produtos diet e light, escolhidos aleatoriamente em estabelecimentos comerciais da cidade. As 12 marcas dos produtos foram codificadas em A, B, C, D, E e F para os alimentos diet e 1, 2, 3, 4, 5 e 6 para os light. Utilizou-se tabelas de modelo check list, referentes às informações nutricionais/obrigatórias. Deste modo, os resultados foram organizados em tabelas diferentes. De acordo com os resultados, as 12 marcas analisadas demonstraram conformidade com a legislação vigente, RDC n° 360, 259 e a portaria n° 27 e 29, Porém 75% das marcas não apresentaram a medida caseira em suas embalagens. Com isso, podemos observar a importância de tais informações para os consumidores sobre a leitura dos rótulos, realizando uma boa escolha do que será consumido.

Introduction Primary Bilateral Macronodular Adrenal Hyperplasia (PBMAH) is a heterogeneous disease characterized by bilateral adrenal macronodules responsible for adrenal Cushing. To date, two genetic causes of PBMAH are known: germline inactivating variants of the tumor suppressor genes ARMC5 identified in 2013 (Assié, N Eng J Med 2013), responsible for 20 to 25% of index cases, and KDM1A, identified recently (Vaczlavik, GIM 2021; Chasseloup, Lancet D&E 2021), responsible for the rare presentation associated with food-dependent Cushing's syndrome (FDCS) due to aberrant expression of the GIP receptor (GIPR) in adrenocortical cells. Multiple other illegitimate receptors are known to be responsible for abnormal cortisol response to various physiological stimuli in PBMAH. A recent multiomic analysis, identified three distinct molecular PBMAH groups: G1 with ARMC5-mutated tumors, G2 with KDM1A-mutated tumors from FDCS patients, and G3 with no identified genetic cause at present. We aimed to identify specific expression profiles of illegitimate receptors in the three groups. Methods Based on the transcriptome data obtained by RNA sequencing (Illumina) of the tumors from 31 patients (G1/ARMC5, 16 patients; G2/KDM1A, 6 patients; G3, 9 patients), expression of the following genes, encoding potential illegitimate receptors, were compared: ADRA1A, ADRA1B, ADRA1D, ADRA2A, ADRA2B, ADRA2C, ADRB1, ADRB2, ADRB3, AVPR1A, AVPR1B, AVPR2, GCGR, GIPR, HTR4, HTR7, LHCGR. Calculations were performed using R statistical software. The Bioconductor limma package was used to analyze mRNA differential expression. Results G1/ARMC5 tumors showed a relative overexpression of the vasopressin receptors AVPR1A and AVPR1B compared to the two other groups (fold-change [FC] =7.39, p Conclusion This study reveals specific expression profiles of illegitimate receptors related to the three molecular groups. ARMC5 tumors are associated with the overexpression of two vasopressin receptors, while, besides GIPR, KDM1A inactivation seems to drive the overexpression of the LH/hCG receptor, as previously suggested in patients with FDCS (Bertherat, JCE&M 2005), potentially responsible for Cushing's syndrome associated with pregnancy and menopause. These molecular patterns need to be corroborated by clinical data with a systematic testing of the aberrant cortisol responses. Additionally, further studies would be needed to investigate the clinical relevance and significance of moderate fold-changes in gene expression (e.g. Presentation: Saturday, June 11, 2022 12:00 p.m. - 12:15 p.m.

Primary bilateral macronodular adrenal hyperplasia (PBMAH) is an adrenal cause of Cushing syndrome. Nowadays, a PBMAH diagnosis is more frequent than previously, as a result of progress in the diagnostic methods for adrenal incidentalomas, which are widely available. Although some rare syndromic forms of PBMAH are known to be of genetic origin, non-syndromic forms of PBMAH have only been recognized as a genetic disease in the past 10 years. Genomics studies have highlighted the molecular heterogeneity of PBMAH and identified molecular subgroups, allowing improved understanding of the clinical heterogeneity of this disease. Furthermore, the generation of these subgroups permitted the identification of new genes responsible for PBMAH. Constitutive inactivating variants in ARMC5 and KDM1A are responsible for the development of distinct forms of PBMAH. To date, pathogenic variants of ARMC5 are responsible for 20-25% of PBMAH, whereas germline KDM1A alterations have been identified in90% of PBMAH causing food-dependent Cushing syndrome. The identification of pathogenic variants in ARMC5 and KDM1A demonstrated that PBMAH, despite mostly being diagnosed in adults aged 45-60 years, is a genetic disorder. This Review summarizes the important progress made in the past 10 years in understanding the genetics of PBMAH, which have led to a better understanding of the pathophysiology, opening new clinical perspectives.

Ceratocystis wilt, caused by Ceratocystis fimbriata, is an important disease that causes the decline and death of mango trees. Root infection by soil inoculum is common, and in such cases, the use of resistant plants is recommended. Because many commonly used cultivars are susceptible to the pathogen, an alternative method to manage the disease is needed, such as the use of Trichoderma spp. as a biological control agent. This study evaluated the potential of Trichoderma spp. to control C. fimbriata in the soil. Eight strains of Trichoderma were isolated from the rhizosphere of mango trees. Three known species were identified: Trichoderma harzianum (TF10 and TF12), Trichoderma longibrachiatum (TF1, TF2, TF5, and TF7) and Trichoderma asperelloides (TF4 and TF6). All eight strains inhibited C. fimbriata in dual culture assays. Trichoderma harzianum and T. asperelloides produced thermostable soluble metabolites that inhibited 100% of the pathogen’s mycelial growth while for T. longibrachiatum the inhibition ranged between 44 to 65%. These eight strains were also tested under greenhouse conditions. Trichoderma asperelloides strain TF6 reduced the incidence of the disease in the roots by 88%; TF12 by 52%, TF2, TF4, and TF7 by 40%; and TF1 by only 31%. This is the first report on the antagonistic potential of Trichoderma spp. against C. fimbriata in mango plants. Biocontrol using species of Trichoderma could be a sustainable, economically, and viable alternative for the management of Ceratocystis wilt.

The software industry has experienced the integration of artificial intelligence capabilities into applications, facing new challenges regarding software development. Despite research and industry contributions providing lessons learned and best practices, no study proposed a reference process for developing this type of software, and practitioners still struggle to establish a working process. Through a Grounded Theory study involving practitioners with experience in machine learning (ML) projects, this paper presents an emerging theory of how ML-based systems are developed. The reported results comprise key elements of a reference development process with its respective phases and activities.

Let $M$ be a compact constant mean curvature surface either in $\mathbb{S}^3$ or $\mathbb{R}^3$. In this paper we prove that the stability index of $M$ is bounded below by a linear function of the genus. As a by product we obtain a comparison theorem between the spectrum of the Jacobi operator of $M$ and those of Hodge Laplacian of $1$-forms on $M$., Minor revision + references added

In this paper we show rigidity results for super-solutions to fully nonlinear elliptic conformally invariant equations on subdomains of the standard $n$-sphere $\mathbb S^n$ under suitable conditions along the boundary. We emphasize that our results do not assume concavity assumption on the fully nonlinear equations we will work with. This proves rigidity for compact connected locally conformally flat manifolds $(M,g)$ with boundary such that the eigenvalues of the Schouten tensor satisfy a fully nonlinear elliptic inequality and whose boundary is isometric to a geodesic sphere $\partial D(r)$, where $D(r)$ denotes a geodesic ball of radius $r\in (0,\pi/2]$ in $\mathbb S^n$, and totally umbilical with mean curvature bounded below by the mean curvature of this geodesic sphere. Under the above conditions, $(M,g)$ must be isometric to the closed geodesic ball $\overline{D(r)}$. As a side product, in dimension $2$ our methods provide a new proof to Toponogov's Theorem about the rigidity of compact surfaces carrying a shortest simple geodesic. Roughly speaking, Toponogov's Theorem is equivalent to a rigidity theorem for spherical caps in the Hyperbolic three-space $\mathbb H^3$. In fact, we extend it to obtain rigidity for super-solutions to certain Monge-Amp\`ere equations.

Background: Dental caries comprises the most prevalent chronic disease in children worldwide and is considered a public health problem. Introduction: Few educational oral health applicatio...

In this paper, we prove that there exists a universal constant C, depending only on positive integers $$n\ge 3$$ and $$p\le n-1$$ , such that if $$M^n$$ is a compact free boundary submanifold of dimension n immersed in the Euclidean unit ball $$\mathbb {B}^{n+k}$$ whose size of the traceless second fundamental form is less than C, then the pth cohomology group of $$M^n$$ vanishes. Also, employing a different technique, we obtain a rigidity result for compact free boundary surfaces minimally immersed in the unit ball $$\mathbb {B}^{2+k}$$ .

In this paper we prove classification results to elliptic fully nonlinear conformal equations in certain subdomains of the sphere with prescribed constant mean curvature on its boundary. Such subdomains are the hemisphere (or a geodesic ball in S n ) of dimension n ≥ 2 with prescribed constant mean curvature on its boundary, and annular domains with minimal boundary. Our results extend previous ones concerning more general curvature functions on the eigenvalues of the Schouten tensor than scalar curvature.

Hohenbergia lanata Pereira & Moutinho is an endemic bromeliad of the State of Bahia (Brazil), occurring in an altitudinal transition forest between Caatinga dry woodland and Atlantic Forest. The type material was collected by Roberto Burle-Marx, who introduced it into cultivation in his gardens, but lacking the accurate provenance, only mentioning that it was collected between Milagres and Amargosa municipalities. It was later described by Pereira & Moutinho based on a cultivated specimen. In this work, we present new specimens found in natural populations in an area that coincides with the type locality description, enabling us to expand the description of the species, as well as to provide images of the living plants in the field, adding information about its habitat. The herbarium collections revision and the new population found in the field enabled us to propose an updated conservation status and to better characterize the habit, plant architecture, and to add new morphological comparisons and geographical distribution.

We report on an improved measurement of the $^8$B solar neutrino interaction rate with the Borexino experiment at the Laboratori Nazionali del Gran Sasso. Neutrinos are detected via their elastic scattering on electrons in a large volume of liquid scintillator. The measured rate of scattered electrons above 3 MeV of energy is $0.223\substack{+0.015 \\ -0.016}\,(stat)\,\substack{+0.006 \\ -0.006}\,(syst)$ cpd/100 t, which corresponds to an observed solar neutrino flux assuming no neutrino flavor conversion of $\Phi\substack{\rm ES \\ ^8\rm B}=2.57\substack{+0.17 \\ -0.18}(stat)\substack{+0.07\\ -0.07}(syst)\times$10$^6$ cm$^{-2}\,$s$^{-1}$. This measurement exploits the active volume of the detector in almost its entirety for the first time, and takes advantage of a reduced radioactive background following the 2011 scintillator purification campaign and of novel analysis tools providing a more precise modeling of the background. Additionally, we set a new limit on the interaction rate of solar $hep$ neutrinos, searched via their elastic scattering on electrons as well as their neutral current-mediated inelastic scattering on carbon, $^{12}$C($\nu,\nu'$)$^{12}$C* ($E_{\gamma}$= 15.1 MeV).

A reliable and consistently reproducible technique to fabricate 222Rn-loaded radioactive sources ( ∼ 0.5–1 kBq just after fabrication) based on liquid scintillator (LS), with negligible amounts of LS quencher contaminants, was implemented. This work demonstrates the process that will be used during the Borexino detectors upcoming calibration campaign, where one or several ∼ 100 Bq such sources will be deployed at different positions in its fiducial volume, currently showing unprecedented levels of radiopurity. These sources need to fulfill stringent requirements of 222Rn activity, transparency to the radiations of interest and complete removability from the detector to ensure their impact on Borexino’s radiopurity is negligible. Moreover, the need for a clean, undistorted spectral signal for the calibrations imposes a tight requirement to minimize scintillator quenching agents (”quenchers”) to null or extremely low levels.

Thanks to the progress of neutrino physics, today we are able of exploiting neutrinos as a tool to study astrophysical objects. The latter in turn serve as unique sources of elusive neutrinos, which fundamental properties are still to be understood. This contribution attempts to summarize the latest results obtained by measuring neutrinos emitted from the Sun and geoneutrinos produced in radioactive decays inside the Earth, with a particular focus on a recent discovery of the CNO-cycle solar neutrinos by Borexino. Comprehensive measurement of the pp-chain solar neutrinos and the first directional detection of sub-MeV solar neutrinos by Borexino, the updated 8B solar neutrino results of Super-Kamiokande, as well as the latest Borexino and KamLAND geoneutrino measurements are also discussed.

Borexino is a 280t liquid scintillator detector at the Laboratori Nazionali del Gran Sasso (LNGS), Italy. Its main goal is the precision spectroscopy of solar neutrinos down to energies of 0.19 MeV and for this task it features an unprecedented radio-purity and a high scintillation light yield. The solar neutrinos are measured by the elastic scattering off electrons which induce isotropically emitted scintillation photons as well as a sub-dominant number of Cherenkov photons that are detected by photomulitplier tubes. Here we present the first detection of sub-MeV solar neutrinos using their associated Cherenkov photons in a high light yield liquid scintillator detector. In Borexino electrons with E>0.16MeV produce Cherenkov photons, where the ratio of Cherenkov photons from the neutrino scattered electrons is estimated to be < 1% for all PMT hits, so a typical event by event direction reconstruction is not possible. Instead this analysis looks at the integrated signal of the PMT hits of all detected events by correlating the position of each hit PMT relative to the reconstructed position of the event and the well known position of the Sun. In this way it is possible to measure an angular distribution that shows the statistical contribution of Cherenkov photons from the solar neutrino recoil electrons. Using the Geant4-based Borexino Monte Carlo to produce the expected angular distribution for solar neutrinos and background we have measured 8643 − 2058 +2252 neutrino events out of 19904 total events for an energy region around the 7Be edge between 0.53 MeV and 0.74 MeV.

The Borexino detector, located at the Laboratori Nazionali del Gran Sasso in Italy, is a radiopure 280 ton liquid scintillator detector with a primary goal to measure low-energy solar neutrinos created in the core of the Sun. These neutrinos are a consequence of nuclear fusion reactions in the solar core where Hydrogen is burned into Helium and provide a direct probe of the energy production processes, namely the proton-proton (pp) chain and the Carbon-Nitrogen-Oxygen (CNO) cycle. The fusion of Hydrogen in the case of the CNO cycle, which is expected to contribute in the order of less than 1% to the total solar energy, is catalyzed by Carbon, Nitrogen, and Oxygen directly depending on the abundances of these elements in the solar core. The measurement of CNO neutrinos is challenging due to the high spectral correlation with the decay electrons of the background isotope 210Bi and the pep solar neutrino signal. The experimental achievement of thermal stabilization of the Borexino detector after mid 2016, has opened the possibility to develop a method to constrain the 210Bi rate through its decay daughter and α emitter 210Po which can be identified in Borexino with an efficiency close to 100 percent on an event-by-event basis. Moreover, the flux of pep neutrinos can be constrained precisely through a global analysis of solar neutrino data which is independent of the dataset used for the CNO analysis. This conference contribution is dedicated to the first experimental evidence of neutrinos produced in the CNO fusion cycle in the Sun which is at the same time the dominant energy production mechanism in heavier stars compared to the Sun.

Dinoflagellate taxonomy has been undergoing a comprehensive review in recent decades. Knowledge on Brazilian dinoflagellate flora remains quite incomplete, particularly regarding the size of the country. This study is the first taxonomic survey of freshwater dinoflagellates in the State of Parana, southern Brazil. Forty-one freshwater environments were sampled in Parana and two others on the border between Parana and Santa Catarina during 2010-2015, and 21 dinoflagellate taxa assigned to 13 genera were identified and described based on light (LM) and scanning electron (SEM) microscopy. A new combination is proposed: Parvodinium africanum var. javanicum comb. nov. Three taxa are new records for Brazilian freshwaters: Glenodiniopsis uliginosa, P. africanum var. javanicum and Tyrannodinium edax. Taxonomic and nomenclatural comments about the recorded species as well as their known distribution in Brazil are also given.

This study evaluated the effect of caprine ovarian tissue transportation conditions (medium supplementation and transportation duration) on the morphology, DNA fragmentation and development of cultured and non-cultured preantral follicles. After the fragmentation of ovaries, one fragment was fixed (fresh control) while the remaining slices were placed individually in two different conservation media (Minimal Essential Medium - MEM without supplementation or supplemented MEM, i.e. MEM+) and stored at 35oC for 6 or 12 h without (non-cultured) or with a subsequent 5-day in vitro culture in supplemented α-MEM. After transportation, followed or not by in vitro culture, the fragments were processed for histological and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) examination. For the preserved and non-cultured fragments, the percentages of normal follicles after the storage of ovarian tissue in MEM+ for 6 h and the DNA fragmentation rates after preservation in MEM for 6 h and MEM+ for 6 or 12 h were maintained similar to the fresh control. However, all cultured treatments reduced the proportion of normal follicles and increased the percentage of TUNEL-positive cells as compared to the fresh control and non-cultured treatments. On the contrary, all culture conditions (except after preservation in MEM for 6 h) promoted an increase in primordial follicle activation. In conclusion, the use of an enriched medium (MEM+) during ovary transportation is preferable to maintain satisfactory rates of normal follicles after the preservation of caprine ovarian tissue at 35oC for up to 6 h, without affecting the ability of the primordial follicle to grow in vitro.

We consider surfaces with parallel mean curvature vector field and finite total curvature in product spaces of type \({\mathbb {M}}^n(c)\times {\mathbb {R}}\), where \({\mathbb {M}}^n(c)\) is a space form and characterize certain of these surfaces. When \(n=2\), our results are similar to those obtained in Berard et al. (Ann Glob Anal Geom 16(3):273–290, 1998) for surfaces with constant mean curvature in space forms.

Borexino is running at the INFN Gran Sasso National Laboraries, Italy since 2007. Its technical distinctive feature is the unprecedented ultralow background of the inner scintillating core, which is the foundation for the outstanding achievements accumulated by this experiment. In this contribution, after recalling the main features of the detector, the impressive solar data gathered so far by Borexino will be summarized. Special emphasis will be given to the illustration of the recent release of the fluxes as stemming from the simultaneous real time spectroscopy of the neutrinos from the entire $pp$ nuclear fusion chain in the Sun, opening with the remarkable 2.7% accuracy of the ⁷Be flux the era of precision measurements also in the realm of the sub-MeV solar neutrinos. Such results put Borexino in the unique situation of being the only detector able to perform solar neutrino spectroscopy over the entire solar spectrum; the counterpart of this peculiar status, in the flavor conversion interpretation of the solar neutrino data, is the capability of Borexino alone to perform the full validation across the solar energy range of the MSW-LMA paradigm. This contribution will be concluded with an account of the Borexino accomplishments in the geo-neutrino field, marked by the detection of the geo-neutrino signal with a significance as high as $5.9\sigma$.

The aim of this study was to evaluate follicular survival and development of ovine isolated secondary follicles cultured in medium containing fixed or sequential concentrations of melatonin and further oocyte maturation. Isolated secondary follicles were cultured for 18 days in α-MEM+ alone (control) or with different concentrations of melatonin (100, 500 or 1000 pg/mL) or sequential concentrations of melatonin (Mel Seq: Day 6 = 100; Day 12 = 500; Day 18 = 1000 pg/mL). The percentages of morphologically normal follicles and antral cavity formation increased significantly in 1000 pg/mL melatonin compared to the other treatments. After 18 days, 1000 pg/mL melatonin (Mel 100) showed a greater (P

This study aimed to evaluate the effect of melatonin on the in vitro culture and maturation of isolated sheep early antral follicles. Isolated early antral follicles were cultured for 12 d in α-minimum essential medium (MEM+) alone (control) or α-MEM+ added with fixed different concentrations (100, 500, or 1,000 pg/mL) or a sequential concentration of melatonin (MelSeq; day 6 = 100; day 12 = 500 pg/mL). The percentage of morphologically normal follicles was higher (P 0.05) to the control group and higher (P 0.05) among control, Mel 500, and Mel 1000 treatments. Mel 500 treatment presented a higher percentage of germinal vesicle breakdown oocytes than the control group and similar percentages to the other treatments. Follicles cultured in melatonin followed by oocyte maturation with the addition of 500 pg/mL melatonin in maturation medium showed increased (P

We present the most recent solar neutrino results from the Borexino experiment at the Gran Sasso underground laboratory. In particular, refined measurements of all neutrinos produced in the {\it pp} fusion chain have been made. It is the first time that the same detector measures the entire range of solar neutrinos at once. These new data weakly favor a high-metallicity Sun. Prospects for measuring CNO solar neutrinos are also discussed., Proceedings for the 15th International Workshop on Tau Lepton Physics (TAU2018), 24-28 September 2018, Amsterdam, The Netherlands. arXiv admin note: substantial text overlap with arXiv:1812.02326

In the summer of 2011, in a nursery located in Viçosa City, Minas Gerais State, brownish, necrotic, irregular spots were observed on leaves of Mabea fistulifera Mart. (Euphorbiaceae), an indigenous forest species commonly found in Brazil. Around 6,300 seedlings were evaluated and as many as 60% of them showed disease symptoms, including severe defoliation and plant death. Leaves with coalescing lesions turned papery in texture and had a blighted appearance. Bacterial colonies were isolated from these symptomatic leaves on King B's medium and identified based on biochemical and molecular analysis, as a member of the Enterobacteriaceae family. Like other members of the Enterobacteriaceae family, the bacteria were facultative anaerobic, gram-negative, cream-colored on YDC medium, urease and oxidase negative, as well as catalase and asparagine positive. Bacterial DNA was extracted from pure culture grown overnight in liquid 523 medium at 28°C using the Wizard Genomic DNA Purification kit (Promega) and conserved sequences in 16S rDNA (3) and rpoB (1) were amplified by PCR. The sequence of the 1,300-bp 16S rDNA fragment and the 750-bp rpoB gene were analyzed by NCBI BLAST. Related sequences were aligned and analyzed by ClustalW in MEGA 5 software. Phylogenetic analysis by maximum likelihood, using PAUP version 4.0 and TBR algorithm with 1,000 bootstrap replications, grouped the isolate in a clade with Enterobacter cowanii and the result showed 99% and 98% identity to the 16s rDNA and rpoB, respectively. The isolate clustered closely with the type strain of E. cowanii in both phylogenetic trees constructed. Pathogenicity tests were carried out by inoculating leaves of healthy seedlings either by spraying or cutting with a scissor previously dipped into a 108 CFU/ml bacterial suspension. The experiment was in a completely randomized design, with six replications. A pot with one plant was considered one experimental unit. Control seedlings were sprayed or cut with a scissor treated with saline solution. Prior to and after inoculation, plants were kept in a humid chamber for 24 h at 26°C in the dark and at room temperature. Subsequently, plants were transferred to growth chamber at 26°C, under a 12-h photoperiod (40 μmol/s/m2). Consistent with the symptoms observed originally, 7 days after inoculation, all seedlings developed leaf spots. No characteristic symptoms could be observed in the negative control. Furthermore, Koch's postulates were confirmed by reisolation of the bacterium from symptomatic tissues. In summary, the phenotypic, biochemical, and molecular tests identified the pathogen as E. cowanii. Recently, E. cowanii was isolated from Eucalyptus trees with symptoms of bacterial blight, although its pathogenicity was not demonstrated (2). To the best of our knowledge, this is the first report of a member of the Enterobacteriaceae family causing disease in M. fistulifera. The result has a great importance to better understand the role of E. cowanii as a pathogen-causing disease on a forest species. References: (1) C. L. Brady et al. Syst. Appl. Microbiol. 31:447, 2008. (2) C. L. Brady et al. Lett. Appl. Microbiol. 49:461, 2009. (3) W. G. Weisburg et al. J. Bacteriol. 173:697, 1991.

Ceratocystis wilt in mango, caused by Ceratocystis fimbriata, is a problem in Brazil, Oman and Pakistan. There is a lack of knowledge regarding this disease, including field detection of the pathogen. This study evaluated the detection of C. fimbriata through carrot baits and a semi-selective medium of stem-inoculated 1-year-old mango plants in a greenhouse and naturally infected 15-year-old mango trees in a commercial orchard. The pathogen was detected with the carrot baits from samples collected at the inoculation point in some greenhouse plants but not from the field trees. C. fimbriata was detected using the semi-selective medium up to 16 cm below the inoculation site of the greenhouse plants, in most of the samples taken from the lesion sites in the field trees, and in one of ten asymptomatic trees that were sampled from the orchard. We concluded that semi-selective medium is an effective and rapid method for diagnosis of C. fimbriata. This is useful for epidemiological studies and more importantly, to manage Ceratocystis wilt in the field.

The Borexino detector measures solar neutrino fluxes via neutrino-electron elastic scattering. Observed spectra are determined by the solar-$\nu_{e}$ survival probability $P_{ee}(E)$, and the chiral couplings of the neutrino and electron. Some theories of physics beyond the Standard Model postulate the existence of Non-Standard Interactions (NSI's) which modify the chiral couplings and $P_{ee}(E)$. In this paper, we search for such NSI's, in particular, flavor-diagonal neutral current interactions that modify the $\nu_e e$ and $\nu_\tau e$ couplings using Borexino Phase II data. Standard Solar Model predictions of the solar neutrino fluxes for both high- and low-metallicity assumptions are considered. No indication of new physics is found at the level of sensitivity of the detector and constraints on the parameters of the NSI's are placed. In addition, with the same dataset the value of $\sin^2\theta_W$ is obtained with a precision comparable to that achieved in reactor antineutrino experiments., Comment: 28 pages, 7 figures. Slight modifications in the title, abstract, and conclusion. Few references added. Text expanded for clarity. Accepted in JHEP

International audience; The Borexino detector was built starting from 1996 in the underground hall C of Gran Sasso National Laboratory (LNGS) in Italy under about 1400 m of rock (3800 m.w.e) and it is mostly aimed to the study in real-time of the low-energy solar neutrinos.Since the beginning of data taking, in May 2007, the unprecedented detector radio-purity made the performances of the detector unique: a milestone has been very recently achieved with the measurement of solar pp neutrino flux, providing the first direct observation in real time of the key fusion reaction powering the Sun.In this contribution the most important Borexino achievements to the fields of solar, geo-neutrino and particle physics are reviewed and the future perspectives discussed, emphasizing in particular the unique possibility of Borexino to cover at the end of its program the entire solar neutrino spectrum and to exploit the possible existence of a fourth sterile neutrino (SOX project).

This work describes the construction of a small-scale radio telescope using open source hardware technologies which can be used as a tool in the training of engineers and physicists, as well as in the dissemination of important technologies for space weather monitoring studies, security of radio communication networks and maintenance of essential services for contemporary society.

The latest and more complete geo-neutrinos Borexino results, are briefly presented and discussed. Borexino is a liquid scintillator detector located at the Gran Sasso National Laboratory in Italy, whose primary purpose is the real-time spectroscopy of low energy solar neutrinos. It is the only experiment so far to have provided an evidence of geo-neutrinos existence beyond a 5σ significance level. The geo-neutrinos measurement and analysis, along with implications from the geological point of view, are shortly discussed.

Solar neutrinos have played a central role in the discovery of the neutrino oscillation mechanism. They still are proving to be a unique tool to help investigate the fusion reactions that power stars and further probe basic neutrino properties. The Borexino neutrino observatory has been operationally acquiring data at Laboratori Nazionali del Gran Sasso in Italy since 2007. Its main goal is the real-time study of low energy neutrinos (solar or originated elsewhere, such as geo-neutrinos). The latest analysis of experimental data, taken during the so-called Borexino Phase-II (2011-present), will be showcased in this talk - yielding new high-precision, simultaneous wide band flux measurements of the four main solar neutrino components belonging to the "pp" fusion chain (pp, pep, 7Be, 8B), as well as upper limits on the remaining two solar neutrino fluxes (CNO and hep).

This study evaluated the in vitro development and maturation of ovine oocytes from secondary follicles cultured in serum-free medium containing fixed or sequential concentrations of recombinant human FSH (rhFSH). Follicles were cultured in α-MEM+ alone or with constant (500, 750, or 1,000 ng/mL) or sequential concentrations of rhFSH (seq. 1: day 6 = 500; day 12 = 750; day 18 = 1,000 ng/mL and seq. 2: day 6 = 100; day 12 = 500; day 18 = 1,000 ng/mL). At the end of the experiment, follicular survival was higher (P

International audience; About 99 per cent of solar energy is produced through sequences of nuclear reactions that convert hydrogen into helium, starting from the fusion of two protons (the pp chain). The neutrinos emitted by five of these reactions represent a unique probe of the Sun’s internal working and, at the same time, offer an intense natural neutrino beam for fundamental physics. Here we report a complete study of the pp chain. We measure the neutrino–electron elastic-scattering rates for neutrinos produced by four reactions of the chain: the initial proton–proton fusion, the electron-capture decay of beryllium-7, the three-body proton–electron–proton (pep) fusion, here measured with the highest precision so far achieved, and the boron-8 beta decay, measured with the lowest energy threshold. We also set a limit on the neutrino flux produced by the$^{3}$He–proton fusion (hep). These measurements provide a direct determination of the relative intensity of the two primary terminations of the pp chain (pp-I and pp-II) and an indication that the temperature profile in the Sun is more compatible with solar models that assume high surface metallicity. We also determine the survival probability of solar electron neutrinos at different energies, thus probing simultaneously and with high precision the neutrino flavour-conversion paradigm, both in vacuum and in matter-dominated regimes.

We aimed to study the effects of resveratrol on the morphology, DNA fragmentation, follicular activation and cell proliferation after in vitro culture of ovine ovarian tissue, and to verify if PI3K pathway is involved in resveratrol action in the sheep ovary. Ovaries were collected and divided into fragments. One fragment was fixed for histology (fresh control). The remaining fragments were cultured for 7 days in control medium (α-MEM+ ) alone or with resveratrol (2, 10 or 30 µM). After culture, ovarian tissue was destined to morphological analysis. TUNEL and proliferating cell nuclear antigen (PCNA) analyses were performed in the fresh control, α-MEM+ and 2 µM resveratrol. Inhibition of PI3K activity was performed through pre-treatment with LY294002. The percentage of normal follicles was similar between α-MEM+ and 2 µM resveratrol, and higher than those in other resveratrol treatments. An increase in follicular activation was observed in all treatments compared to fresh control. DNA fragmentation decreased in tissues cultured in 2 µM resveratrol compared to α-MEM+ . Moreover, PCNA-positive cells were higher in 2 µM resveratrol than in α-MEM+ . LY294002 inhibited follicular activation stimulated by α-MEM+ and 2 µM resveratrol. In conclusion, 2 µM resveratrol promotes primordial follicle activation compared to the fresh control by reducing DNA fragmentation and stimulating granulosa cell proliferation through activation of the PI3K pathway.

We prove that there are no minimal hypersurfaces properly immersed in any region of the Euclidean space bounded by unstable minimal cones. We also prove the analogous result for $r$-minimal hypersurfaces., Comment: 7 pages

In this paper we obtain lower bound estimates of the spectrum of the Laplace-Beltrami operator on complete submanifolds with bounded mean curvature, whose ambient space admits a Riemannian submersion over a Riemannian manifold with negative sectional curvature. Our main theorem generalizes many previously known estimates and applies for both immersions and submersions.

Made available in DSpace on 2018-11-26T16:16:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-05-01. Added 1 bitstream(s) on 2019-10-09T18:25:36Z : No. of bitstreams: 1 S0102-695X2015000300228.pdf: 675360 bytes, checksum: ed8bbb1454f9719acc059484c6f335cf (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Arrabidaea brachypoda Bureau, Bignoniaceae, known as cipo-una, is widely used in traditional medicine in Southeastern and Northeastern Brazil for kidney stones and painful joints. This study was aimed at evaluating the anti-inflammatory proprieties of the oleanane-type triterpenoid 3 beta-estearioxy-olean-12-ene isolated from the roots of A. brachypoda. Carrageenan-induced paw oedema, formalin test and hot plate test were used to investigate the antiinflammatory activity of 3 beta-estearioxy-olean-12-ene in animals. We observed that 3 beta-estearioxy-olean-12-ene at doses of 5, 10 and 15 mg/kg p.o. demonstrated anti-inflammatory effects, by reduced (p < 0.05) paw oedema induced by carrageenan and by decreased (p < 0.05) licking time caused by a subplantar injection of formalin. In conclusion, 3 beta-estearioxy-olean-12-ene, a triterpene isolated from the roots of A. brachypoda, demonstrate anti-inflammatory effect indifferent tests. Thus, it may be useful in the treatment of inflammatory disorders, which supports previous claims of its traditional use. (C) 2015 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved. Univ Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, SP, Brazil Univ Fed Alfenas, Inst Ciencias Biomed, Dept Ciencias Fisiol, Alfenas, MG, Brazil Soc Brasileira Fisiol, Programa Multictr Posgrad Ciencias Fisiol, Sao Paulo, SP, Brazil Univ Fed Vicosa, Dept Quim, Vicosa, MG, Brazil Univ Estadual Paulista, Inst Quim, Dept Quim Organ, Araraquara, SP, Brazil

We report the first results of DarkSide-50, a direct search for dark matter operating in the underground Laboratori Nazionali del Gran Sasso (LNGS) and searching for the rare nuclear recoils possibly induced by weakly interacting massive particles (WIMPs). The dark matter detector is a Liquid Argon Time Projection Chamber with a (46.4+-0.7) kg active mass, operated inside a 30 t organic liquid scintillator neutron veto, which is in turn installed at the center of a 1 kt water Cherenkov veto for the residual flux of cosmic rays. We report here the null results of a dark matter search for a (1422+-67) kg d exposure with an atmospheric argon fill. This is the most sensitive dark matter search performed with an argon target, corresponding to a 90% CL upper limit on the WIMP-nucleon spin-independent cross section of 6.1x10^-44 cm^2 for a WIMP mass of 100 GeV/c^2., 26 pages, 7 figures; updated figures and references, notably Fig. 7 has updated CDMSII curve; fixed typos; clarified some language and figures; updated references

In this paper we prove compactness theorems for weighted manifolds under suitable assumptions on their generalized Ricci curvatures and in the sense of the classical Myers compactness theorem. As applications we present new closures theorems for space-like hypersurfaces in some space-time manifolds.