Search

IntroductionSoil polluted with Nickel (Ni) adversely affects sunflower growth resulting in reduced yield. Counterbalancing Ni toxicity requires complex molecular, biochemical, and physiological mechanisms at the cellular, tissue, and whole plant levels, which might improve crop productivity. One of the primary adaptations to tolerate Ni toxicity is the enhanced production of antioxidant enzymes and the elevated expression of Ni responsive genes.MethodsIn this study, biochemical parameters, production of ROS, antioxidants regulation, and expression of NRAMP metal transporter genes were studied under Ni stress in sunflower. There were four soil Ni treatments (0, 50, 100, and 200 mg kg-1 soil), while citric acid (CA, 5 mM kg-1 soil) was applied on the 28th and 58th days of plant growth. The samples for all analyses were obtained on the 30th and 60th day of plant growth, respectively.Results and discussionThe results indicated that the concentrations of Ni in roots and shoots were increased with increasing concentrations of Ni at both time intervals. Proline contents, ascorbic acid, protein, and total phenolics were reduced under Ni-stress, but with the application of CA, improvement was witnessed in their contents. The levels of malondialdehyde and hydrogen peroxide were enhanced with the increasing concentration of Ni, and after applying CA, they were reduced. The contents of antioxidants, i.e., catalase, peroxidase, superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase, were increased at 50 ppm Ni concentration and decreased at higher concentrations of Ni. The application of CA significantly improved antioxidants at all concentrations of Ni. The enhanced expression of NRAMP1 (4, 51 and 81 folds) and NRAMP3 (1.05, 4 and 6 folds) was found at 50, 100 and 200ppm Ni-stress, respectively in 30 days old plants and the same pattern of expression was recorded in 60 days old plants. CA further enhanced the expression at both developmental stages.ConclusionIn conclusion, CA enhances Ni phytoextraction efficiency as well as protect plant against oxidative stress caused by Ni in sunflower.

Soil polluted with Nickel (Ni) adversely affects sunflower growth resulting in reduced yield. Counterbalancing Ni toxicity requires complex molecular, biochemical, and physiological mechanisms at the cellular, tissue, and whole plant levels, which might improve crop productivity. One of the primary adaptations to tolerate Ni toxicity is the enhanced production of antioxidant enzymes and the elevated expression of Ni responsive genes.In this study, biochemical parameters, production of ROS, antioxidants regulation, and expression ofThe results indicated that the concentrations of Ni in roots and shoots were increased with increasing concentrations of Ni at both time intervals. Proline contents, ascorbic acid, protein, and total phenolics were reduced under Ni-stress, but with the application of CA, improvement was witnessed in their contents. The levels of malondialdehyde and hydrogen peroxide were enhanced with the increasing concentration of Ni, and after applying CA, they were reduced. The contents of antioxidants, i.e., catalase, peroxidase, superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase, were increased at 50 ppm Ni concentration and decreased at higher concentrations of Ni. The application of CA significantly improved antioxidants at all concentrations of Ni. The enhanced expression ofIn conclusion, CA enhances Ni phytoextraction efficiency as well as protect plant against oxidative stress caused by Ni in sunflower.

Soil receiving discharges from Pb-acid batteries dismantling and restoring units (PBS) can have a high concentration of phytoavailable Pb. Reducing Pb phytoavailability in PBS can decline Pb uptake in food crops and minimize the risks to humans and the environment. This pot study aimed to reduce the concentration of phytoavailable Pb in PBS through Aspergillus niger (A. niger)-mediated release of PO

Since Ni-rich soils are a threat to the environment, growing edible crops on Ni-rich soils can pose a serious risk to human, animal, plant and ecosystem health and, hence, is considered as a challenging task for the researchers. Contrarily, limiting the bioavailability of Ni in such soils upon the addition of suitable amendments cum foliar spray of proteinogenic amino acids having an objective to alleviate stress to crop plants can considerably reduce the environmental risk. In this pot trail, we substantiate the effects of biochar (BR) and zeolite (ZL) addition in the soil along with proline (PN) spray on the resistance, and stress responses of wheat against Ni as well as on Ni translocation and accumulation in wheat plants grown on a Ni-rich soil contaminated by electroplating effluent. The treatments, applied with and without PN spray, involved: no amendment; BR; ZL; and a concoction of both amendments (BR50%+ZL50%). We found that BR50%+ZL50% treatment significantly immobilized Ni in the soil, reduced its accumulation in the shoot, root, and grain, blocked membrane lipid peroxidation and showed an improvement in photosynthetic parameters, the status of antioxidant activities, grain biochemistry and grain yield, compared to the control. Interestingly, exogenous PN spray caused a significant additive effect on the aforementioned parameters in the wheat plants grown on BR50%+ZL50% treated soil. Our results involved a reduced Ni bioavailability in wheat rhizosphere due to BR50%+ZL50% in soil and, furthermore, the additive effect of PN spray to scavenging ROS, obstructing peroxidation of lipid membrane and, thus providing resilience to wheat plant against Ni stress. The suggested technique can make Ni-rich soils suitable for cultivation and production of high-quality food by minimizing Ni bioavailability and toxicity to plants.

Elevation of arsenic (As) toxicity is becoming a threat to the environment due to its elevated levels in the soil, water, vegetables, and crops worldwide. Arsenic strongly interacts with iron (Fe), sulfur (S), and phosphorus (P) in agronomic soils. Iron and S strongly bind As in soils and thus reduce As bioavailability for plant uptake. The presence of phosphate [PO4]− 3 triggers As transportation that results in the accumulation of high concentrations of As in rice and other crops. Arsenate [As(V)] is a phosphate [PO4]− 3 analog that enters plant cell via phosphate transport system and interrupts phosphate metabolism. Plants do not have such a mechanism to cope with As(V) uptake in roots. So the applications of P fertilizers to restrict As loading in plants is not a good practice. Sulfur plays a major part in As detoxification by forming arsenite [As(III)] complex with thiol peptides. This complex formation binds As in the roots of plants and resists As translocation. Likewise, Fe also plays a crucial role in the biogeochemical processes of As with Fe oxyhydroxides on the soil and root surface of wetland plants acting as a strong adsorbent for As. Oxidation of Fe(II) and precipitation of Fe(III) on the root surface produce Fe plaques in the rhizosphere of rice. The application of ferrous sulfate also reduces As toxicity to rice, resulting in high grain nutritional yield and decrease grain As loading.

Approximately, more than 200 million hectares are contaminated with heavy metals (HMs) having very high concentrations greater than the standard values worldwide. Thus, the urgent remediation of HMs contaminated soils is the need of the hour. In situ immobilization of HMs through organic, inorganic, and other stabilizing additives seems to be the most promising remediation technique in managing HMs pollution. The efficiency of different stabilizing agents has been previously tested for the rehabilitation of HMs contaminated soils with the immediate estimation of their leaching and availability from them. Among tested amendments, biochar and iron base amendments have shown their high efficiency in removing multi-HMs polluted soils. Thus, the immobilization technique seems to be a preferable alternative over other traditional remediation methods owing to its vast applicability, easy availability of raw materials, and wide acceptability. However, weathering activities may increase the risk of HMs remobilization due to the breakdown of organic amendments. Thus continuous monitoring of HMs soils is recommended.

Military firing or shooting ranges exhibit the most significant environmental concern of the current era. The copresence of diverse types of organic (TNT, RDX, and HMX) and inorganic pollutants (Pb, Cu, Cd, Ni, Cr, Zn, As, and Sb) can cause endless damage to the environment. Therefore the presence of these pollutants at a single site makes them a good option for studying the behavior and transformation mechanisms of both types of pollutants. Traditional remediation approaches can render the toxicity of pollutants at these sites. However, they are not preferred due to their high cost, low public acceptability, indirect harm to the environment, and more labor involvement. Contrary to it, such sites that are highly contaminated with organic pollutants, metals, and metal(loid)s can be managed by using various approaches like bioremediation, bacterial degradation/transformation, phytoremediation, phytoextraction, immobilization, and combination of these techniques. Likewise the sites polluted with organic explosives, strategies like phytotransformation, bioremediation, bioaugmentation, and photolysis are recommended, which can reduce the levels of toxicants by their transformation, degradation, and conversion into nontoxic forms, whereas phytoextraction, bacterial transformation, in situ immobilization, and phytostabilization can deem the risk of inorganic pollutants at such sites. By undertaking these techniques and management practices, the harm to the environment from these polluted shooting ranges can be scaled down.

Rhizosphere acidification in leguminous plants can release P from the dissolution of phosphate compounds which can reduce Pb bioavailability to them via the formation of insoluble Pb compounds in their rhizosphere. A soil polluted from Pb-acid batteries effluent (SPBE), having total Pb = 639 mg kg−1, was amended with six different rates (0, 0.5, 1, 2, 4 and 6%) of oxalic acid-activated phosphate rock (OAPR) and their effects on pH, available P and bioavailable Pb concentrations in the rhizosphere and bulk soils of mung bean plant were evaluated. Furthermore, the effects of these variant OAPR rates on Pb concentrations in plant parts, bioaccumulation factor (BAF) and translocation factor (TF) for Pb in grain and traits like productivity, the activities of antioxidant enzymes, and grain biochemistry were investigated. Results revealed that increasing rates of OAPR significantly increased pH values and available P while decreased bioavailable Pb concentrations in the rhizosphere over control. The highest dissolution of P in the rhizosphere was with 4 and 6% OAPR rates. As a result, the formation of insoluble Pb compounds affected on reduced Pb concentrations in shoots, roots, and grain in addition to lower grain BAF and TF values for Pb over control. Likewise, the highest plant productivity, improved grain biochemistry, high Ca and Mg concentrations, least oxidative stress, and enhanced soil alkaline phosphatase activity were found with 4 and 6% OAPR rates. The OAPR 4% rate is suggested for reducing grain Pb concentration, cell oxidative injury, and improving grain biochemistry in mung bean.

Soil pollution with Cd has promoted serious concerns for medicinal plant quality. Amending Cd-polluted soils with textile waste biochar (TWB) coated with natural polymers can lower Cd bioavailability in them and reduce associated environmental and human health risks. In this study, we explored the impacts of solely applied TWB, chitosan (CH), their mix (TWB + CH) and TWB coated with CH (TBC) in Cd-polluted soil on Cd distribution in moringa (Moringa oleifera L.) shoots and roots as well as plant-available Cd in soil. Moreover, amendments effects on plant growth, dietary quality, and antioxidative defense responses were also assessed. Results revealed that the addition of TWB, CH, and TWB + CH in Cd-polluted soil reduced Cd distribution in shoots (56%, 66%, and 63%), roots (41%, 48%, and 45%), and plant-available Cd in soil (38%, 52%, and 49%), compared to control. Interestingly, the TBC showed significantly the topmost response for reducing Cd concentrations in shoots, roots, and soil by 73%, 54%, and 58%, respectively, relative to control. Moreover, amending Cd-polluted soil with TWB, CH, and TWB + CH depicted significantly better effects on plant growth, dietary quality, and activities of soil enzymes but the topmost response was observed with TBC treatment. Compared with control, TBC improved plant growth parameters: shoot length (81%), root length (90%), shoot fresh weight (60%), root fresh weight (76%), shoot dry weight (75%), root dry weight (68%) contents of chlorophyll-a (42%) and chlorophyll-b (74%), and soil enzyme activities: urease (130%), catalase (138%), protease (71%), cellobiohydrolase (45%), acid phosphatase (34%), peroxidase (60%), β-glucosidase (152%), chitinase (62%), and phosphomonoesterase (139%). Furthermore, TBC treatment arrested Cd-induced oxidative stress via escalating the activities of antioxidant enzymes as well as improved moringa dietary parameters (protein, tannins, lipids, alkaloids, saponins, terpenoids, flavonoids, and tocopherols contents). Such findings suggest that the TBC has an immense perspective to remediate Cd-polluted soils and prevent human health risks associated with Cd exposure through the diet.

Silicon is known to improve resistance against salinity stress in maize crop. This study was conducted to evaluate the influence of silicon application on growth and salt resistance in maize. Seeds of two maize genotypes (salt-sensitive ‘EV 1089’ and salt-tolerant ‘Syngenta 8441’) were grown in pots containing 0 and 2 mM Si with and without 50 mM NaCl. After detailed investigation of ion concentrations in different maize organs, both genotypes were further selected in hydroponic experiment on basis of their contrasting response to salinity stress. In the second experiment, pre-germinated seedlings were transplanted into nutrient solution with 0 and 60 mM NaCl with and without 2 mM Si. Both genotypes differed significantly in their response to salinity. Silicon addition alleviated both osmotic and oxidative stress in maize crop by improving the performance of defensive machinery under salinity stress. Silicon application also improved the water-use efficiency in both tested genotypes under both normal and salinity stress conditions. In conclusion, this study implies that the silicon-treated maize plants had better chance to survive under salinity conditions and their photosynthetic and biochemical apparatus was working far better than that of silicon-non-treated plants.

This study demonstrates the reduction of nickel (Ni) toxicity and enhancing zinc (Zn) bioavailability from sunflower seed with biochar (BC) and chitosan (CH) application to Ni contaminated soil. Th...

The complex interaction of biochar (BC) with soil health reflecting properties, the feedstock used to prepare BC and application rate of BC in sandy soil is still a question for the researchers. An incubation study was conducted where nine different sorts of BC, each prepared from the different feedstock, were applied at 2% rate to evaluate their relative suitability to improve sandy soil health. Results revealed that BC prepared from miscanthus (MIB) significantly increased soil medium and fine pores, available water content (AWC), electrical conductivity (EC), and cation exchange capacity (CEC) while decreased soil wide pores, pH, bulk density (BD) and particle density (PD) compared to the rest sorts of BC. Later, spinach was grown in pots containing same soil but spiked with 50 ppm nickel (Ni) and amended with 1, 2, 3, 4 and 5% rates of MIB. The results showed a significant increment in spinach biomass, reduction in the concentrations of Ni in spinach tissues and DTPA-extractable Ni with the increasing rate of MIB till 3% and later, no significant changes with 4 and 5% rates thereafter. However, significant improvement in the activities of antioxidant enzymes, chemical and biochemical attributes of spinach were observed at 5% MIB when compared to lower rates. Similarly, post-harvest soil physicochemical and enzymatic parameters were also significantly (P < 0.05) improved with increasing rates of MIB. This study implies that application of MIB at 5% rate can improve the nutritional quality of spinach, sandy soil health and can reduce Ni concentrations in spinach tissues.

In situ immobilization of Cd is desirable due to the damaging effects of ex situ remediation techniques on soil. In this greenhouse study, the role of biochar (BC), chitosan (CH), and green waste (GW) was studied for in-situ Cd immobilization and alleviating Cd toxicity in mung bean seedlings. Amendments were applied at rates of 0.5% and 1% (w/w). The minimum mean value of Cd, in root, shoot, and soil (DTPA-Cd) (12.2, 4.7, and 0.7 mg kg −1 , respectively), occurred in the Cd + 1% CH treatment compared to all Cd amended treatments. Shoot dry weight (21%) increased significantly in Cd + 1% BC amended soil compared to the control. Reactive oxygen species were affected significantly, with the lowest increased value of hydrogen peroxide (4%) in the Cd + 1% CH treatment while the minimum increase in the value of superoxide (O 2 •– ) occurred in the Cd + 1% BC soil compared to the control. Malondialdehyde (20%) increased lowest with Cd + 1% CH treatment. Protein, ascorbate (AsA) contents, and catalase (CAT) activity increased the most (3, 2, and 15%, respectively) in the Cd + 1% BC treatment while dehydroascorbate reductase (DHAR) and superoxide dismutase (SOD) activity increased the most (9 and 234%, respectively) in the Cd + 1% CH soil compared to the control. Glutathione reductase (GR), ascorbate peroxidase (APX), and glutathione peroxidase (GPX), activity were reduced the most in the Cd + 1.0% BC treatment while dehydroascorbate (DHA) and glutathione S -transferase (GST) activity decreased the most in the Cd + 1% CH soil. Overall, in situ immobilization by amendments improved growth and antioxidant defense mechanisms of mung bean seedlings and was reflected by tolerance to Cd-toxicity.

Quinoa (Chenopodium quinoa Willd.) is a traditional Andean agronomical resilient seed crop having immense significance in terms of high nutritional qualities and its tolerance against various abiotic stresses. However, finite work has been executed to evaluate the growth, physiological, chemical, biochemical, antioxidant properties, and mineral nutrients bioavailability of quinoa under abiotic stresses. Depending on the consistency in the stability of pH, intended rate of S was selected from four rates (0.1, 0.2, 0.3, 0.4 and 0.5% S) for the acidification of biochar and compost in the presence of Thiobacillus thiooxidans by pH value of 4. All three soils were amended with 1% (w/w) acidified biochar (BCA) and compost (COA). Results revealed that selective plant growth, yield, physiological, chemical and biochemical improved significantly by the application of BCA in all stressed soils. Antioxidants in quinoa fresh leaves increased in the order of control > COA > BCA, while reactive oxygen species decreased in the order of control

A restricted dietary range and a deficit of essential minerals such as zinc (Zn) characterize the diets of under-nourished people. Zn deficiency is a global nutritional problem and intensity of the...

Soil factors such as pH, calcium, carbonate, and bicarbonate precipitation products in calcareous soils reduce iron (Fe) availability to crops and limit grain Fe concentrations. In the present greenhouse study, we evaluated the potential of Fe fertilizer amendments combined with organic amendments, like biochar (BC) and poultry manure, in sulfur (S)-treated low pH calcareous soils (pHS1) to assess Fe biofortification of maize. Elemental sulfur (S) was used both for lowering soil pH and Fe solubilization. Soil pH was successfully lowered down from 7.8 to 6.5 by S application at the rate of 2.5 g kg−1 soil. Pot experiment results revealed that Fe fertilizer combined with BC and S (pHS1) significantly increased root and shoot dry weight, grain weight, photosynthetic rate, transpiration rate, and stomatal conductance by 69%, 86%, 28%, 74%, 57%, and 33%, respectively, relative to the control. Similarly, combined application of Fe + BC in S-amended (pHS1) soil increased starch (34%), protein (64%), and ...

Calcareous soil, high pH, and low organic matter are the major factors that limit iron (Fe) availability to rice crop. The present study was planned with the aim to biofortified rice grain with Fe, by integrated use of chemical and organic amendments in pH-manipulated calcareous soil. The soil pH was reduced (pH

Soil salinity imposes an unprecedented risk to the soil fertility and availability of plant nutrients. The present proposal is designed to address the effect of salt stress on photosynthetic apparatus of maize including chlorophyll a fluorescence and how silicon nutrition helps to overcome this issue. In a sand culture experiment, two maize cultivars were sown in small pots with two levels of silicon (0 and 2 mM H2SiO3) and two levels of salinity stress (0 and 60 mM NaCl). Salinity stress reduced dry matter yield and potassium (K) concentration in both maize cultivars and also induced inefficient working of photosynthetic apparatus including photochemical efficiency of photosystem II. Silicon addition alleviated NaCl stress on maize crop by improving the dry matter yield and water use efficiency (WUE). It decreased shoot Na concentration by increasing root and shoot K concentration of maize plants. It enhanced maximum quantum yield of primary photochemistry which leads to smooth electron transport...

Incidence of iron (Fe) deficiency in human populations is an emerging global challenge. This study was conducted to evaluate the potential of iron sulphate combined with biochar and poultry manure for Fe biofortification of wheat grains in pH affected calcareous soil. In first two incubation studies, rates of sulfur (S) and Fe combined with various organic amendments for lowering pH and Fe availability in calcareous soil were optimized. In pot experiment, best rate of Fe along with biochar (BC) and poultry manure (PM) was evaluated for Fe biofortification of wheat in normal and S treated low pH calcareous soil. Fe applied with BC provided fair increase in root-shoot biomass and photosynthesis up to 79, 53 and 67%, respectively in S treated low pH soil than control. Grain Fe and ferritin concentration was increased up to 1.4 and 1.2 fold, respectively while phytate and polyphenol was decreased 35 and 44%, respectively than control in treatment where Fe was applied with BC and S. In conclusion, combined use of Fe and BC could be an effective approach to improve growth and grain Fe biofortification of wheat in pH affected calcareous soil.

Iron (Fe) bioavailability is the major issue of highly calcareous soils. Iron fertilization in calcareous soils is not much effective due to their rapid conversion into unavailable form. A field experiment was conducted to evaluate the effect of Fe fertilizer along with organic amendments for improving growth, yield and Fe biofortification of rice by manipulating pH of calcareous soil. Before transplanting rice seedlings, soil pH was lowered down up to 0.5-0.6 units by using 0.25% (w/w) elemental sulfur (S). For biofortification, Fe fertilizer (FeSO4.7H2O) at the rate of 15 kg ha -1 was applied with biochar (BC) and poultry manure (PM) in S treated calcareous soils. Results indicated that the combined application of Fe along with BC and PM enhanced plant growth, physiology and paddy yield and improved nutritional value of grain in pH manipulated calcareous soil. Applied treatments in S treated low pH soil resulted in increased Fe mobilization and translocation from root to grain. Grain Fe concentration raised up to 2 fold and 1.8 fold in treatments where Fe was applied with BC and PM in pH manipulated soil, respectively. Ferritin concentration significantly increased up to 3 fold and 2.73 fold by using Fe with BC and PM, respectively, in pH manipulated soil. Iron applied in combination with BC decreased phytate and polyphenols contents up to 18 and 47%, respectively, in S treated soil. The results may imply that the combined use of Fe with BC increased Fe biofortification (ferritin) of rice in pH manipulated calcareous soil. This increased level of ferritin in rice grains might be helpful to eliminate Fe deficiency in humans. Keyword: Biofortification, iron, zinc, ferritin, protein, phytate, polyphenols, rice

While disobeying environmental regulations of Pakistan, several Pb-acid batteries recycling and repairing units discharge their effluents into water canals that irrigate arable fields. Resultantly, serious ecological risks, as well as human health hazards through consumption of edible crops grown on such Pb-polluted soils have been reported. In this experiment, we observed associative effects of amending a soil polluted from Pb-acid batteries effluents (SPB) with arbuscular mycorrhizal fungi (AMF) and lignin-derived biochar (LBC) on barley grain safety to human health. The SPB was treated with AMF inoculum (a consortium of four AMF species), lignin (LN), and LBC, as sole treatments and AMF inoculum with LN and LBC. Barley parameters involving Pb distribution in grain and other parts, grain biochemistry, and nutrition were assessed. Likewise, Pb bioavailability in SPB, AMF root colonization, soil enzymes, microbial biomass carbon (MBC), and AMF produced total glomalin related soil protein (TGSP) were also scoped. Additionally, human renal cells (HEK 293) cytotoxicity test was performed by opting barley grain-related Pb concentrations. Results show that LBC + AMF significantly reduced grain Pb concentrations below the critical limit [4.67 mg kg−1 (WHO/FAO standard)], AMF colonization, MBC, soil enzymology, and TGSP, compared to control. Likewise, rest barley parameters were also improved in this treatment. Contrary to other treatments, grain produced on LBC + AMF did not result in (a) cell apoptosis, (b) cell distortion and (c) cohesion loss. Immobilization of Pb in SPB was due to the dilution effect of Pb adsorption on LBC, AMF mycelium and TGSP which resulted in a significant drop of grain Pb concentrations below the critical limit and ultimately no harm to HEK 293 cells. Our findings endorse that grain produced at LBC + AMF treatment are safer for human consumption and will not pose health risks. The LBC + AMF application can remediate SPB for safer cereal production.

Nickel (Ni) contaminated soils pose a potential ecological risk to the environment, soil health, and quality of food produced on them. We hypothesized that application of miscanthus biochar (BC) and cationic zeolite (ZE) at various proportions into a Ni contaminated soil can efficiently immobilize Ni and reduce its bioavailability to sunflower (Helianthus annuus L.) and maize (Zea mays L.). An electroplating effluent contaminated soil was amended with BC and ZE, as sole treatments (2% w/w) and their combinations of various ratios (BC, ZE, BC25%ZE75%, BC50%ZE50% and BC75%ZE25%) for immobilization of Ni in the soil. Furthermore, the associated effects of these treatments on residual and DTPA-extractable Ni from the soil; concentrations of Ni in shoots, roots, and grain; growth, physiology, biochemistry and the antioxidant defence mechanisms of sunflower and maize were investigated. Results revealed that BC50%ZE50% treatment efficiently reduced DTPA-extractable Ni in the soil, Ni concentrations in shoots, roots, and grain, while improved selective parameters of both plants. Interestingly, the BC75%ZE25% treatment significantly improved the biomass, grain yield, physiology, biochemistry and antioxidant defense machinery, while decreased Ni oxidative stress in both sunflower and maize, compared to rest of the treatments. The results demonstrate that the BC50%ZE50% treatment can efficiently reduce Ni concentrations in the roots, shoots and grain of both sunflower and maize whereas, an improvement in biomass, grain yield, physiological, biochemical, and antioxidant defense machinery of both crops can only be achieved with the application of BC75%ZE25% treatment in a Ni contaminated soil.

Rice is an important crop and the food security of the world is strongly associated with it as it is the staple food of half of the world’s population. Among various agro-management practices seedling age and nitrogen rates significantly affected its growth, development and yield components. Rice cultivars performed differently when transplanted in field at varying seedling ages depending upon their genetic makeup and adoptability to certain environmental conditions. Seedling age plays an important role in yield contributing parameters like number of productive tillers, panicle length, filled grains panicle-1 and 1000-kernel weight leading to higher paddy yield in different rice cultivars and hybrids. Nitrogen is required in huge quantity in rice production as it is an important constituent of plant parts and processes. Paddy yield increases significantly with the increase in nitrogen rate but after a certain limit yield starts decreasing. Keeping in view the significance of seedling age and nitrogen rates in different rice cultivars and hybrids, an effort has been made to review some research work already conducted and will be helpful to the researchers and scientists to plan future strategies.

Depleting aquifers, lack of planning and low socioeconomic status of Pakistani farmers have led them to use wastewater (WW) for irrigating their crops causing food contamination with heavy metals and ultimately negative effects on human health. This study evaluates the effects of chitosan (CH) and biochar (BC) on growth and nutritional quality of brinjal plant together with in situ immobilization of heavy metals in a soil polluted with heavy metals due to irrigation with wastewater (SPHIW) and further irrigated with the same WW. Both CH and BC were applied at three different rates i.e. low rate [(LR), BC0.5%, CH0.5% and BC0.25%+CH0.25%], medium rate [(MR), BC1%, CH1% and BC0.5%+CH0.5%] and high rate [(HR), BC1.5%, CH1.5% and BC0.75%+CH0.75%]. Result revealed that brinjal growth, antioxidant enzymes, and fruit nutritional quality significantly improved from LR to HR for each amendment, relative to control. However, these results were more prominent with BC alone and BC+CH, compared with CH alone at each rate. Similarly, with few exceptions, significant reduction in Ni, Cd, Co, Cr and Pb concentrations in the root, shoot and fruit were found in sole CH treatment both at LR and MR but in both CH and BC+CH treatments at HR, relative to control. Interestingly, the concentrations of Fe in the roots, shoots and fruit were more pronounced at BC treatments relative to CH and BC+CH treatments at each rate, compared to control. Overall, the BC+CH treatment at HR was the most effective treatment for in situ immobilization of heavy metals in SPHIW and further irrigated with the same WW, compared to rest of the treatments. This study indicates that BC0.75%+CH0.75% treatment can be used to reduce mobility and bioavailability of heavy metals in SPHIW and facilitates plant growth by improving the antioxidant system. However, the feasibility of BC0.75%+CH0.75% treatment should also be tested at the field scale.

Rice ( Oryza sativa L.) is one of the main staple food crops which is inherently low in micronutrients, especially iron (Fe), and can lead to severe Fe deficiency in populations having higher consumption of rice. Soils polluted with nickel (Ni) can cause toxicity to rice and decreased Fe uptake by rice plants. We investigated the potential role of biochar (BC) and gravel sludge (GS), alone and in combination, for in situ immobilization of Ni in an industrially Ni-contaminated soil at original and sulfur-amended altered soil pH. Our further aim was to increase Fe bioavailability to rice plants by the exogenous application of ferrous sulfate to the Ni-immobilized soil. Application of the mixture of both amendments reduced grain Ni concentration, phytate, Phytate/Fe, Phyt/Zn molar ratios, and soil DTPA-extractable Ni. In addition, the amendment mixture increased 70 % Fe and 229 % ferritin concentrations in rice grains grown in the soil at original pH. The Fe and ferritin concentrations in S-treated soil was increased up to 113 and 383 % relative to control respectively. This enhanced Fe concentration and corresponding ferritin in rice grains can be attributed to Ni/Fe antagonism where Ni has been immobilized by GS and BC mixture. This proposed technique can be used to enhance growth, yield, and Fe biofortification in rice by reducing soil pH while in parallel in situ immobilizing Ni in polluted soil.

Food security is a serious issue in this era of rapidly growing population. Food security is threatened due to low crop yields around the world due to different biotic and abiotic stresses. The arable lands are decreasing due to different soil degradation process and thus prospect for increasing crop yields through extending areas under cultivation are not very bright. Hence, to achieve the food security on a sustainable basis, it is necessary to utilize degraded soils productively. Among the degraded soils, Salt-affected soils share the major fraction and their presence is prevalent in all continents. The adoption of different management techniques for productive use of salt-affected soils is thus pre-requisite for enhancing crop yields on such soils. These management strategies include management of irrigation water, reclamation techniques, raising beds, organic matter and salt tolerant crops like kallar grass. In addition to these, better fed plants have good potential to withstand with salinity and other stresses. Exogenous application of nutrients can alleviate the detrimental effects of salts. Silicon (Si) as a beneficial nutrient is known to improve plant growth particularly under abiotic stresses. It is helpful for plants in many ways as it improves plant water status in the context of relative water content and transpiration rate. The role of Si to promote the plant growth under salt-affected soils is reviewed in detail in this chapter.

Iron (Fe) deficiency is a prevalent nutritional deficiency throughout the world, affecting an estimated 3.7 billion people. Increasing Fe concentration in foodcrops is an important global challenge due to the high incidence of Fe deficiency in human population. Further, cereals grown on calcareous soil are low in Fe contents. High pH, high temperature, low organic matter and poorly managed soil with respect to fertility are the factors which cause low Fe availability to cereal crops in calcareous soil. Iron fertilization in calcareous soil is not effective due to their rapid conversion into unavailable forms and poor mobility of Fe in phloem. Iron-organic compounds in manure are effective in maintaining Fe availability to plants. Fe bioavailability inhibitors such as polyphenols and phytate inhibit iron absorption but it was concluded that their inhibitory effect on iron absorption can be limited by increasing iron content. Ferritin, an iron storage protein, can deposit thousands of iron atoms asnon-toxic form and ferritin iron is bioavailable to humans as ferrous sulphate. Improving both concentration and bioavailability of Fe in cereal grains is, therefore, an important challenge and a high-priority research area. Hence, there is a need for effective strategies to overcome Fe deficiency in cereals and to increase Fe bioavailabilty in cereals grains. Biofortification of food crops with Fe to combat iron deficiency problems in humans, is a cost-effective and sustainable agricultural strategy to alleviate malnutrition. We hypothesized that Fe nutrition management in calcareous soil can increase growth, yield and Fe bioavailability from cereals.

Calcareous soil, high pH, and low organic matter are the major factors that limit iron (Fe) availability to rice crop. The present study was planned with the aim to biofortified rice grain with Fe, by integrated use of chemical and organic amendments in pH-manipulated calcareous soil. The soil pH was reduced (pHL2) by using elemental sulfur (S) at the rate of 0.25 % (w/w). The organic amendments, biochar (BC) and poultry manure (PM) [1 % (w/w)], along with ferrous sulfate at the rate of 7.5 mg kg−1 soil were used. The incorporation of Fe with BC in soil at pHL2 significantly improved plant biomass, photosynthetic rate, and paddy yield up to 99, 97, and 36 %, respectively, compared to control. A significant increase in grain Fe (190 %), protein (58 %), and ferritin (400 %) contents was observed while anti-nutrients, i.e., polyphenols (37 %) and phytate (21 %) were significantly decreased by the addition of Fe and BC in soil at pHL2 relative to control. Among the organic amendments, PM significantly increased Cd, Pb, Ni, and Cr concentrations in rice grain relative to control but their concentration values were below as compared to the toxic limits of hazard quotients and hazard index (HQ and HI). Hence, this study implies that Fe applied with BC in the soil at pHL2 can be considered as an effective strategy to augment Fe bioavailability and to reduce non-essential heavy metal accumulation in rice grain.