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Your search keyword '"Piyarat Thaijaroen"' showing total 2 results
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2 results on '"Piyarat Thaijaroen"'

1. First Asian, Single-Center Experience of Blastocyst Preimplantation Genetic Diagnosis with Human Leukocyte Antigen Matching in Thailand for the Prevention of Thalassemia and Subsequent Curative Hematopoietic Stem Cell Transplantation of Ten Affected Siblings

Author

Kasorn Tiewsiri, Warachaya Sutheesophon, J Marshall, Piyarat Thaijaroen, Pagawadee Ketcharoon, Cara K. Bradley, Steven J. McArthur, Preeda Vanichsetakul, Konstantinos I. Papadopoulos, Somjate Manipalviratn, and Weena Krutsawad

Subjects
lcsh:R5-920, lcsh:Cytology, business.industry, Thalassemia, medicine.medical_treatment, Cell Biology, General Medicine, Hematopoietic stem cell transplantation, Preimplantation genetic diagnosis, medicine.disease, Single Center, medicine.anatomical_structure, Human Leukocyte Antigen Matching, Immunology, Medicine, Scientific Abstracts, Blastocyst, lcsh:QH573-671, lcsh:Medicine (General), business, Developmental Biology
Published
2019
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2. Human sex determination by amelogenin padlock probes

Author

Nathinee Panvisavas, Piyarat Thaijaroen, Rapee Boonplueang, and Korapin Srisiri

Subjects
Genetics, chemistry.chemical_compound, chemistry, Rolling circle replication, Degraded dna, Amelogenin, Biology, Indel, Molecular biology, AMELX, DNA, Pathology and Forensic Medicine
Abstract

In this study, we utilized a technique called ligation-mediated rolling circle amplification (L-RCA) that combined padlock probe ligation and rolling circle amplification techniques to determine human sex. Amelogenin X and Y specific padlock probes were designed based on polymorphisms and the 6-bp indel of the amelogenin X and Y alleles. Lengths of DNA target for padlock probe detection were 40 and 33 bases of amelogenin X and Y alleles, respectively. Analysis of female DNA samples produced one L-RCA product from the AmelX padlock probe, whereas 2 different types of products were amplified from male DNA samples, each generated by AmelX and AmelY padlock probes. Utilization of padlock probes via L-RCA technique would provide an alternative tool for human sex determination in highly degraded DNA analysis in the future.

Published
2011
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