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In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co-fed from first-feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP-90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in, This study was partially supported by the projects SAARGO – POCI/MAR/6123/2004 and HYDRAA – PTDC/MAR/71685/2006, granted by Fundação para a Ciência e Tecnologia, Portugal. NR, M de V and TSS acknowledge financial support from Fundação para a Ciência e Tecnologia, Portugal, through grants SFRH/BDP/34888/2007, SFRH/BD/40698/2007 and SFRH/BD/41392/2007 respectively.

Trabajo presentado en el V Workshop The cultivation of the Soles, celebrado en Faro (Portugal) del 5 al 7 de abril de 2011., Low sperm volume and quality is pointed out as a major reason for the low fertilization rates observed in Senegalese sole (Solea senegalensis) broodstocks both wild and F1. R ecent studies by our group have shown that the poor and variable sperm quality is related to the high sensibility of plasma membrane to seawater during fertilization. Diet enrichment in fatty acids and cholesterol could change the composition of the sperm membrane and the addition of antioxidants would protect sperm to peroxidation.Therefore, in the present work we tested specific diets enriched with DHA, selenium and Vit. E, aiming to improve sperm sole quality., J. Beirão is supported by PhD grant (SFRH/BD/31990/2006) from the Portuguese Ministry of Science and Technology.

Trabajo presentad en el World Aquaculture 2011, celebrado en Natal (Brasil) del 6 al 10 de junio de 2011., Currently, several species of grouper are already being produced in Asia for commercial purposes. Several individuals are kept as broodstock in research facilities throughout the Mediterranean.

Trabajo presentado en el 3rd International Workshop on Biology of Fish Gametes, celebrado en Budapest del 7 al 9 de septiembre de 2011., Cryopreservation of testicular germ cells offers a tool for the conservation and production of species with potential for aquaculture, due to the capacity of spermatogonia differentiating into gametes. These cellshave the ability to proliferate in a host individual after transplantation producing, in their gonad, functionalgametes depending on the host sex determination (Yoshizaki et al., 2011). Cryopreservation andtransplantation of testicular germ cells have been attempted in several mammalian species (Redden et al., 2009) and more recently in some fish (Takeuchi et al . 2009; Yoshizaki et al., 2011). In the present studycryopreservation protocols were developed for testicular germ cells from Senegalese sole ( Solea senegalensis ), a species with several reproductive problems, with the aim to transplant into newly hatched larvae and testtheir efficiency., This research was supported by the Spanish Ministry of Science and Innovation through project PGCFISH(AGL2008-02172) and ASSEMBLE call; E. Cabrita was supported by a research contract (RYC-2007-01650); T. Pacchiarini was supported by a JAE-DOC fellow (CSIC).

Earthen ponds are the main production system for seabass and seabream in Portugal and in Southern Spain, in particular in Cádiz province. Different farms use various levels of stocking densities and pond sizes, but in general these are semi-intensive systems covering large areas with ponds ranging from one to several hectares and production levels from 0.5 to 6 Kg/m3 (mostly below 2 kg/m3) at the end of the production cycle., This research has been carried out with the financial support from the Commission of the European Communities, specific RTD programme “Specific Support to Policies”, SSP-2005-44483 “SEACASE - Sustainable extensive and semi-intensive coastal aquaculture in Southern Europe”, and does not necessarily reflect the European Commission views and in no way anticipates the Commission’s future policy in this area.

Earthen ponds are the main production system for seabass and seabream in Portugal and in Southern Spain, in particular in Cádiz province. Different farms use various levels of stocking densities and pond sizes, but in general these are semi-intensive systems covering large areas with ponds ranging from one to several hectares and production levels from 0.5 to 6 Kg/m3 (mostly below 2 kg/m3) at the end of the production cycle. Although seabass and seabream are traditionally the target species produced in such ponds, there is commonly natural recruitment of wild larvae of other fish species, including Senegalese sole. Previous attempts at ongrowing sole in ponds with seabass and seabream as added value gave varied results in terms of number of individuals recovered, but sole growth rates were promising. Polyculture of seabass and seabream is already an established practice, with proportions of 4:1 normally used. In seabass dominated ponds seabream is used to control growth of macroalgae and to clean the ponds. When seabream production dominates, the carnivorous nature of seabass is used to control populations of smaller fish which enter the ponds naturally and might compete for feed. Polyculture of species from different trophic levels has also been considered an efficient and environmentally sound strategy to minimise the impacts of aquaculture systems, since an important fraction of dissolved nutrients and organic matter is recycled within the pond (Buschmann et al. 1996, Sorgeloos 2001, World Bank 2006)., This research has been carried out with the financial support from the Commission of the European Communities, specific RTD programme “Specific Support to Policies”, SSP-2005-44483 “SEACASE - Sustainable extensive and semi-intensive coastal aquaculture in Southern Europe”, and does not necessarily reflect the European Commission views and in no way anticipates the Commission’s future policy in this area.

A 65-day experiment was conducted to determine survival, growth, feed utilization and oogenesis of Nereis diversicolor (O. F. Müller, 1776), fed with a commercial dry diet developed for gilthead seabream Sparus auratus L., 1758. Worms were reared in three replicate tanks with 50 worms per tank (255 indiv · m_2). Tetramin®, a commonly used diet for ornamental fish and polychaete laboratory experiments, was used as a control. Survival was high (95.3 %) for both treatments, and not significantly affected by diet. Specific growth rates and feed efficiency ratios did not differ significantly between worms fed with either diets, nor were there significant differences in the proportion of individuals in the oocyte size classes., La supervivencia, el crecimiento, la utilización del alimento y la oogénesis de Nereis diversicolor (O. F. Müller, 1776) alimentado con pienso de dorada Sparus auratus L., 1758 fueron estudiados durante 65 días. Los poliquetos se cultivaron en tres tanques replicados con 50 individuos en cada uno (255 indiv · m_2). Como control se utilizó Tetramin®, una dieta común para peces ornamentales y en experimentos de laboratorio con poliquetos. La supervivencia fue alta (95,3 %) en ambos tratamientos y no se vio afectada significativamente por la dieta. El aumento de peso y los cocientes de eficiencia alimentaria no variaron significativamente entre los poliquetos alimentados con ambas dietas. Entre los grupos experimentales no hubo diferencia significativa en la proporción de individuos por clases de tamaño de los oocitos., Instituto Español de Oceanografía

The growth and survival of the polychaete Nereis diversicolor (O. F. Müller, 1776) fed on faeces of the carpet shell clam Ruditapes decussatus (L., 1758) were studied under controlled temperature and salinity conditions. Juvenile worms were fed with clam R. decussatus faeces in the presence or absence of sediment during 65 days. Starvation treatments were tested as a control. The results of the present study show that the worms were able to grow using the clam faeces in the presence or absence of sediment. However, the worms from the trials with sediment showed a higher survival rate and biomass production than those from the trials in which no sediment was used. These findings suggest that a suspension of bivalve faeces can be used in the rearing of juvenile N. diversicolor in an integrated polyculture system., El crecimiento y la supervivencia del poliqueto Nereis diversicolor (O. F. Müller, 1776) alimentado con heces de almeja Ruditapes decussatus (L., 1758) fueron estimados bajo condiciones controladas de temperatura y salinidad. Los poliquetos juveniles fueron alimentados con heces en presencia y ausencia de sedimento durante 65 días. Como control se aplicaron tratamientos de ayuno. Los resultados sugieren que los poliquetos crecieron usando las heces como única dieta en presencia y en ausencia de sedimento. Es más: los poliquetos de los ensayos con sedimiento mostraron supervivencia y producción de biomasa mayores que aquéllos en que no se utilizó sedimiento. Los resultados sugieren que se puede usar una suspensión de heces procedentes de bivalvos en el cultivo de juveniles de N. diversicolor en un sistema de policultivo integrado., Instituto Español de Oceanografía

The growth and survival of the polychaete Nereis diversicolor (O. F. Müller, 1776) fed on faeces of the carpet shell clam Ruditapes decussatus (L., 1758) were studied under controlled temperature and salinity conditions. Juvenile worms were fed with clam R. decussatus faeces in the presence or absence of sediment during 65 days. Starvation treatments were tested as a control. The results of the present study show that the worms were able to grow using the clam faeces in the presence or absence of sediment. However, the worms from the trials with sediment showed a higher survival rate and biomass production than those from the trials in which no sediment was used. These findings suggest that a suspension of bivalve faeces can be used in the rearing of juvenile N. diversicolor in an integrated polyculture system. El crecimiento y la supervivencia del poliqueto Nereis diversicolor (O. F. Müller, 1776) alimentado con heces de almeja Ruditapes decussatus (L., 1758) fueron estimados bajo condiciones controladas de temperatura y salinidad. Los poliquetos juveniles fueron alimentados con heces en presencia y ausencia de sedimento durante 65 días. Como control se aplicaron tratamientos de ayuno. Los resultados sugieren que los poliquetos crecieron usando las heces como única dieta en presencia y en ausencia de sedimento. Es más: los poliquetos de los ensayos con sedimiento mostraron supervivencia y producción de biomasa mayores que aquéllos en que no se utilizó sedimiento. Los resultados sugieren que se puede usar una suspensión de heces procedentes de bivalvos en el cultivo de juveniles de N. diversicolor en un sistema de policultivo integrado. Instituto Español de Oceanografía

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