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Background: Gastric cancer (GC) ranks fifth among all common malignancies globally. Genetic research has revealed several genes that are frequently dis-regulated in GC, such as lysine-specific demethylase 6A (KDM6A) and cadherin-1 (CDH1). Objective: This study aimed to examine the expression profile and role of KDM6A in GC, as well as the molecular pathway involved. Methods: The expression profile and overall survival data of KDM6A were retrieved from the TCGA database. Expression levels of KDM6A were also measured in GC patient samples and compared with those of healthy controls. Furthermore, stable silencing of KDM6A was introduced into the GC cell line NCI-N87, followed by assessments of cell proliferation, migration and invasion, in the xenograft mouse model. The metastatic status of mice injected with NCI-N87 cells was also analyzed. Results: In patients diagnosed with GC, KDM6A was upregulated. Silencing KDM6A reduced the proliferation, migration and invasion of cells, as well as the growth of xenograft tumors. KDM6A knockdown also inhibited metastatic behaviors of injected NCI-N87 cells, as well as elevated CDH1 expression, leading to reversed epithelial-mesenchymal transition. Conclusion: KDM6A serves as an oncogene in GC and exerts its pro-tumor functions by repressing the expression of CDH1.

Research on coagulation dysfunction following burns is controversial. This study aimed to describe the coagulation changes in severe burn patients by examining coagulation parameters.Patients with third-degree total body surface area (TBSA) burns of ≥30% were enrolled between 2017 and 2020. Platelet (PLT) count and coagulation indexes (including APTT, INR, FIB, DD, and AT Ⅲ) were measured at admission and once weekly for 8 weeks, and statistical analysis was performed. The patient medical profiles were reviewed to extract demographic and clinical data, including TBSA, third-degree TBSA, and inhalation injury. The total intravenous fluids and transfusions of crystalloids, fresh frozen plasma (FFP), and red blood cells (RBC) were calculated during the forty-eight-hour period. The number of sepsis cases was recorded.We enrolled 104 patients , and while the overall coagulation trend fluctuated, inflection points appeared around one week and demonstrated hypercoagulability. INR was significantly higher in the non-survival group than in the survivors' group from admission to three weeks after burn (all p0.01). From post-injury week 1 to post-injury week 3, the APTT in the non-survival group was greater than in the survival group, but the non-survival group's PLT count was lower than that in the survival group (all p0.05). At two and three weeks after burns, the FIB levels in the non-survival group were significantly lower than those of the survival group (both p0.01). The prevalence of inhalation injury and the proportion of sepsis cases were significantly higher in the non-survival group than in the survival group ( p ＜ 0.05, p ＜ 0.001, respectively). At the time of death, APTT, INR, and FDP levels were significantly higher in the non-survival group in the survivor group, and FIB, ATIII, and PLT were significantly lower than in the survivor group (all p0.01). On the day of death, nine of the 12 dead patients had disseminated intravascular coagulation (DIC).Coagulation dysfunction was most prominent in severe burn patients 1 week after injury and presented as hypercoagulability. Large-area burn injury, large amounts of fluid resuscitation, inhalation injury, and sepsis may all contribute to coagulation dysfunction, which can further develop into DIC and even death in severe burns patients.

BACKGROUND: LncRNA STK4 antisense RNA 1 (STK4-AS1) has been identified as a potential biomarker associated with multiple cancers. We proposed that STK4-AS1 plays a role in the proliferation of osteosarcoma by regulating the cell cycle. METHODS: We compared the expression of STK4-AS1, p53, and p21 in osteosarcoma vs normal samples in clinical tissues and cell lines. We determined the effect of overexpression and knockdown of STK4-AS1 in p53 expressing osteosarcoma cells U2OS, p53 muted osteosarcoma cells MG63, and osteoblast cells hFOB on p53 and p21 expression and the cell viability. For U2OS and MG63, the cell cycle was analyzed and the expression of cyclin proteins was determined. We overexpressed p53 or p21 in STK4-AS1 overexpressed cells to explore the association of STK4-AS1 and p53 in U2OS. RESULTS: The STK4-AS1 expression was higher and p53 and p21 expression were lower in osteosarcoma tissue and cells than in their non-cancer counterparts. The expression of STK4-AS1 was negatively correlated with the expression of p53 or p21. Knockdown of STK4-AS1 in U2OS decreased the cell viability, increased cells in the G0/G1 phase, decreased cells in the S and G2/M phase, decreased expression of cyclin A and B, increased p53 and p21, and had no effect on cyclin D and cyclin E, while overexpression of STK4-AS1 did the opposes. Overexpression of p53 or p21 recovered some changes caused by STK4-AS1 overexpression in U2OS. MG63 expressed no p53 and the expression of p21, cyclin A, and cyclin B, cell viability, and cell cycle were not affected by altered STK4-AS1 levels. In hFOB cells, the expression of p53 and p21 was decreased and the cell viability was increased when STK4-AS1 was overexpressed, but they were not affected when STK4-AS1 was knocked down. CONCLUSION: LncRNA STK4-AS1 promoted the cell cycle of osteosarcoma cells by inhibiting p53 expression.

Direct functionalization of the C(O)–N amide bond is one of the most high-profile research directions in the last few decades; however oxidative couplings involving amide bonds and functionalization of thioamide C(S)–N analogues remain an unsolved challenge.

Boronic acids as a tunable hydroxy source display a unique reactivity paradigm through hitherto unknown aryne-induced multicomponent reactions.

With China's continuous opening to the outside world, changes in the international environment and the operation of the cross-border RMB settlement system (CIPS), the scale of cross-border RMB settlement has fluctuated continuously. In response to this phenomenon, the authors collected and sorted out the total amount of RMB cross-border settlement and payments from 2011 to 2020 time sequence data in China, then use five AR models including ARMA, GARCH(1.1), EGARCH(1.1), PARCH(1.1), and CARCH(1.1) to fit. The experimental results show that the four autocorrelation models all prove that the cross-border RMB settlement has autocorrelation relationship, and the long-term trend continues to grow up. According to the precision and accuracy of the five models, the ARMA model equation is one optimal prediction equation. On the basis of the ARMA model equation, and the establishment of the VENSIM system dynamics simulation model, the scale of China's cross-border RMB settlement in the next 10 years is predicted.

Iridium-based electrocatalysts remain the only practical anode catalysts for proton exchange membrane (PEM) water electrolysis, due to their excellent stability under acidic oxygen evolution reaction (OER), but are greatly limited by their high cost and low reserves. Here, we report a nickel-stabilized, ruthenium dioxide (Ni-RuO

The ECOWAS region has one of Africa's highest potentials for energy production, including both non-renewable (oil, gas, and uranium) and renewable sources (hydroelectric, solar energy, wind energy). Despite this significant potential, the region deals with a number of issues that affect its energy strategy. A review and analysis of the social, political, and economic factors influencing regional energy policy are provided in this paper, along with an assessment and forecast of energy policy in the ECOWAS. The analysis of regional energy policy then takes into account demand management, clean energy production, regional energy trade, and hydrocarbon exploration and production. The results show that the ECOWAS nations have started their transition to a renewable energy-based economy. These policies have long-term implications on the world's energy system and have the potential to improve the region's energy policy, even if the consequences are not immediately noticeable.

Long noncoding RNA (lncRNA) CBR3-AS1 (termed as CBR3-AS1) has been reported to be upregulated in several cancers including osteosarcoma. Its positive impact on the proliferation, migration, and invasion of osteosarcoma cells has been unveiled; nevertheless, whether it also affects the stemness and epithelial-mesenchymal transition (EMT) of osteosarcoma cells is unclear. The purpose for this study was to explore the effects of CBR3-AS1 on the stemness and EMT of osteosarcoma cells as well as its underlying mechanism. qRT-PCR and western blot were applied to detect target gene expression. Function assays were conducted to evaluate the effect of genes on the stemness and EMT of osteosarcoma cells. Mechanism assays were done to verify the association among different genes.

Separation of americium (Am) from curium (Cm) could greatly facilitate the development of advanced nuclear fuel cycles, help with accurate nuclear forensic analysis, and allow for more efficient recovery and utilization of the two strategic elements. In this work, an Am/Cm separation strategy based on the stabilization of pentavalent Am in a biphasic solvent extraction system using bismuthate as the oxidant and

Reticulon 3 (RTN3) is an endoplasmic reticulum protein that has previously been shown to play roles in neurodegenerative diseases, but little is known about its function in the kidneys. The aim of the present study was to clarify the roles of RTN3 in chronic kidney disease (CKD) and kidney fibrosis. In this study, RTN3 levels were measured in kidney tissues from healthy controls and CKD or kidney fibrosis patients. An RTN3-null mouse model was generated to explore the pathophysiological roles of RTN3 in the kidneys. The underlying mechanisms were studied in primary proximal tubular epithelial cells and HEK293 cells in vitro. The results showed that (1) a reduction in RTN3 in mice induces CKD and kidney fibrosis; (2) decreased RTN3 expression is found in patients with CKD; (3) RTN3 plays critical roles in regulating collagen biosynthesis and mitochondrial function; and (4) mechanistically, RTN3 regulates these phenotypes by interacting with GC-Rich Promoter Binding Protein 1 (GPBP1), which activates the IGF2-JAK2-STAT3 pathway. Our study indicates that RTN3 might play crucial roles in CKD and kidney fibrosis and that a reduction in RTN3 in the kidneys might be a risk factor for CKD and kidney fibrosis.

Research on the enrichment mechanism of lithium in coal can increase its economic value. We applied a series of techniques such as X-ray diffraction and ICP-MS to analyze the coal samples collected in the Bijie area and studied the enrichment mechanism of lithium in coal based on the theory of mineralogy and sedimentology. The results show that (1) the highest abundance of lithium in coal is 222 μg/g, the lowest 33.2 μg/g, and the average 87.05 μg/g. Among them, the abundance of lithium in coal samples from Xinhua coalfield and Wenjiaba coalfield reached 136 and 222 μg/g, respectively, which reached the minimum industrial grade (120 μg/g). (2) Lithium in coal was positively correlated not only with ash (0.46) and clay minerals (0.41) but also with total organic matter (0.38) and volatile matter (0.58) in coal, indicating that lithium in coal in the Bijie area has both inorganic and organic origins. (3) The ratio of TiO

Multi-photoaddressable systems (MPSs) belong to complex systems, which are comprised of more than one photoswitching molecule and can respond to different wavelengths of light simultaneously. While MPSs have been extensively applied in various fields, there are also some challenges, such as the deficiency of the wavelength-selective control and the interference from the poor thermodynamic stability of used photoswitching molecules. Herein, we reported two robust MPSs (MPS1/2) consisting of diarylethylene derivative (DAE) and different donor-acceptor Stenhouse adducts (DASAs), in which both opened and closed forms of DAE and opened forms of DASAs are thermodynamically stable. MPS1/2 enable fully reversible cyclic photoswitching with improved thermal interference resistance. Moreover, MPS2 also shows a favorable property in PMMA films and has been applied in multicolor display. It is expected that the prepared MPSs could be used in more fields such as information storage and reading and encoding light.

Background. Uncut Roux-en-Y gastrojejunostomy, recently developed in China, is useful in the treatment of distal gastric cancer. This study is aimed at comparing laparoscopic gastric jejunum uncut Roux-en-Y anastomosis with conventional anastomosis in the surgical treatment of distal gastric malignancy. Methods. In this retrospective study, the clinical data of 178 patients and their follow-up records were analyzed. 112 cases (uncut group) were the observation group for stomach jejunum uncut Roux-en-Y anastomosis, the control group for the stomach, 66 cases (conventional group) were for jejunum Roux-en-Y anastomosis and Billroth I and Billroth II anastomosis. A comparison between the two groups was conducted based on the general situation of the patients, TNM stage, and one-year survival rate. Results. There was no significant difference reported between the two groups in terms of the general situation and TNM stage. A comparison on postoperative complications between the two groups revealed that the postoperative bleeding was 0.9% and 6.1%, the bile reflux gastritis was 1.8% and 9.1%, the anastomotic leakage was 0.0% and 3.0%, the delayed gastric emptying was 0.9% and 7.6%, and the overall complications was at 3.6% and 25.8%, which was significantly lower in the observation group than in the control group, and the difference was statistically significant. Notably, there was no significant difference in 1-year survival rate between the two groups. Conclusion. Laparoscopic gastric jejunal uncut Roux-en-Y anastomosis significantly reduces the risk of postoperative complications of the digestive tract. Its operation is easy and exhibits an effective curative effect.

Lanthanide/actinide separation is a worldwide challenge for atomic energy and nuclear waste treatment. Separation of americium (Am), a critical actinide element in the nuclear fuel cycle, from lanthanides (Ln) is highly desirable for minimizing the long-term radiotoxicity of nuclear waste, yet it is extremely challenging given the chemical similarity between trivalent Am(III) and Ln(III). Selective oxidation of Am(III) to a higher oxidation state (OS) could facilitate this separation, but so far, it is far from satisfactory for practical application as a result of the unstable nature of Am in a high OS. Herein, we find a novel strategy to generate stable pentavalent Am (Am(V)) through coordination of Am(III) with a diglycolamide ligand and oxidation with Bi(V) species in the presence of an organic solvent. This strategy leads to efficient stabilization of Am(V) and an extraordinarily high separation factor (10

Supramolecular host–guest systems are generally at thermodynamic equilibrium states or in kinetically trapped states. Herein, we demonstrate the concept of a chemical fuel-driven competitive host–g...

Understanding the mechanisms, structuring microbial communities in oligotrophic ocean surface waters remains a major ecological endeavor. Functional redundancy and metabolic tuning are two mechanisms that have been proposed to shape microbial response to environmental forcing. However, little is known about their roles in the oligotrophic surface ocean due to less integrative characterization of community taxonomy and function. Here, we applied an integrated meta-omics-based approach, from genes to proteins, to investigate the microbial community of the oligotrophic northern Indian Ocean. Insignificant spatial variabilities of both genomic and proteomic compositions indicated a stable microbial community that was dominated by Prochlorococcus, Synechococcus, and SAR11. However, fine tuning of some metabolic functions that are mainly driven by salinity and temperature was observed. Intriguingly, a tuning divergence occurred between metabolic potential and activity in response to different environmental perturbations. Our results indicate that metabolic tuning is an important mechanism for sustaining the stability of microbial communities in oligotrophic oceans. In addition, integrated meta-omics provides a powerful tool to comprehensively understand microbial behavior and function in the ocean.

Comparison of the extraction and separation properties between two novel phenanthroline-derived organophosphorus ligands, Et-Ph-BPPhen and Et-Ph-PIPhen.

The state-of-the-art method generating potential functions used in molecular dynamics is based on machine learning with neural networks, which is critical for molecular dynamics simulation. This method provides an efficient way for fitting multi-variable nonlinear functions, attracting extensive attention in recent years. Generally, the quality of potentials fitted by neural networks is heavily affected by training datasets and the training process and could be ensured by comprehensively verificating the model accuracy. In this study, we obtained the neural network potential of face-centered cubic (FCC) Cu with the most accurate and adequate training datasets from first-principle calculations and the training process performed by Deep Potential Molecular Dynamics (DeePMD). This potential could not only succeed in reproductions of the variety of properties of Cu at 0 K, but also have a good performance at finite temperatures, such as predicting elastic constants and the melting point. Moreover, our potential has a better generalization capacity to predict the grain boundary energy without including extra datasets about grain boundary structures. These results support the applicability of the method under more practical conditions.

Objective To investigate the predictive value of aspartate aminotransferase/alanine aminotransferase ratio (SLR) and globulin/prealbumin ratio (GPR) in the prognosis of patients with gastric cancer. Methods The patients with gastric cancer initially diagnosed at the Affiliated Hospital of Guizhou Medical University from January 2014 to December 2018 were retrospectively analyzed for peripheral blood aspartate aminotransferase and alanine aminotransferase ratio (AST/ALT) and globulin and prealbumin ratio (GLO/PA) serological levels at the time of initial diagnosis, and ROC curves were used to obtain the optimal threshold values of AST/ALT and GLO/PA . Survival rates were compared using Kaplan-Meier survival analysis, and Cox regression models were used for univariate and multivariate analyses, with differences considered statistically significant at PResults The threshold value of SLR was calculated from the ROC curve as 1.433, and the threshold value of GPR was 0.109. Univariate analysis showed that the poor prognostic factors affecting the 3-year OS rate of gastric cancer patients included T grouping, N grouping, M grouping, clinical-stage grouping, SLR grouping, GPR grouping, and tumor size grouping, and the differences were statistically significant (P 1.433 were independent risk factors for poor prognosis of 3-year OS in gastric cancer patients. It was further used to construct the nomogram prediction chart, calculate the c-index and depict the calibration curve to judge the accuracy of the model, and depict the decision curve to judge the predictive ability of the model. Conclusion The nomogram prediction chart including SLR can more accurately predict the prognosis of gastric cancer patients.

Several recent studies suggested that TLKs are related to tumor progression. However, the function and action mechanism of TLK2 in GC remain elusive. We observed that TLK2 was significantly upregulated in GC and acted as an independent prognostic factor in GC patients. In line with these results, TLK2 silencing markedly reduced GC aggressiveness, whereas its overexpression had the opposite effect. IP-MS suggested that the effect of TLK2 expression on GC was mainly associated with metabolism reprogramming. TLK2 knockdown suppressed amino synthesis through downregulating the mTORC1 pathway and ASNS expression in GC. Mechanistically, mTORC1 directly interacted with ASNS and inhibited its protein degradation. Further experiments confirmed that ASNS protein degradation was ubiquitination degradation rather than degradation through autophagy. Inhibiting and activating the mTORC1 pathway can upregulate and downregulate ASNS ubiquitination, respectively. Moreover, the mTORC1 pathway can reverse the protein regulation of ASNS by TLK2. Notably, TLK2 regulated the ASNS mRNA levels. Further evidence confirmed that TLK2 directly interacted with ATF4, a transcription factor of ASNS, and promoted the expression of ATF4. Finally, we found kinase inhibitor Fostamatinib can significantly inhibit the proliferation, invasion, and migration of GC cells by inhibiting TLK2 enzyme activity. Taken together, this study reveals a novel functional link between TLK2, the mTORC1/ASNS axis, and GC. Therefore, TLK2 could be a potential therapeutic approach for GC target therapy.

Most structural and evolutionary properties of galaxies strongly rely on the stellar initial mass function (IMF), namely the distribution of the stellar mass formed in each episode of star formation. As the IMF shapes the stellar population in all stellar systems, it turns out to become one of the most fundamental concepts of modern astronomy. Both constant and variable IMFs across different environments have been claimed despite a large number of theoretical and observational efforts. However, the measurement of the IMF in Galactic stellar populations has been limited by the relatively small number of photometrically observed stars, leading to high uncertainties. Here we report a star-counting result based on ~93,000 spectroscopically observed M-dwarf stars, an order of magnitude more than previous studies, in the 100--300 parsec (pc) Solar neighbourhood. We find unambiguous evidence of a variable IMF that depends on both metallicity and stellar age. Specifically, the stellar population formed at the early time contains fewer low-mass stars compared to the canonical IMF, independent of stellar metallicities. In present days, on the other hand, the proportion of low-mass stars increases with stellar metallicity. The variable abundance of low-mass stars in our Milky Way establishes a powerful benchmark for models of star formation and can heavily impact results in Galactic chemical enrichment modelling, mass estimation of galaxies, and planet formation efficiency., Comment: Main text (3 Figures, 6 pages) and Supplementary Information (16 pages, 6 Figures). Nature accepted