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Previous work has demonstrated that slightly acidic electrolyzed water (SAEW) can promote growth and nutrient enrichment of buckwheat sprouts. In this study, iTRAQ-based quantitative proteomic analysis of SAEW-induced buckwheat sprouts was conducted to explore its mechanism of action. The results showed that 11, 10 and 14 differentially expressed proteins (DEPs) related to energy metabolism, oxidative stress and flavonoid biosynthesis accumulated upwards and downwards, respectively, in SAEW-treated buckwheat. Bioinformatics analysis revealed 118 GO categories were in relation to molecular function. In the SAEW group, a total of 9 DEPs (5 up-regulated) were mapped to 10 significantly enriched KEGG pathways. SAEW induced flavonoid enrichment by modulating zymoproteins (e.g. phenylalanine ammonialyase and flavonol synthase) in phenylpropanoid biosynthesis pathway. qRT-PCR results had consistency with abundance levels of their corresponding proteins. These findings are likely to reveal the molecular mechanisms underlying the biochemical enrichment of buckwheat sprouts by SAEW.

the present study adopts the research method of spot check and does an analysis on 14 body indexes of boys and girls from 3 to 6 years old, including body build, function, quality and ect. The results are as follows: generally speaking, kids in kindergarten of Urumqi that are up the grade account for 88%, which is 2% higher than the average in Xinjiang but 1.2% lower than the average of China. In the above result, the excellent ones take up 9.8%, which is 0.5% higher than the average in Xinjiang but 1.4% lower than the average of China, the average ones take up 23.8%, which is 0.3% higher than that of Xinjiang but 1.4% lower than that of China, people who fail to pass the grade account for 11.2%, 2% lower than that of Xinjaing but 1.2% higher than that of China. The above results present the growing rules, development trend, differences and reasons of body indexes of kids in Urumqi, which provide some scientific base for the studies on kids' corporeity and its evaluation standards.