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Additional file 1. The following associated content is available online in the supporting information datasheet: Nanoparticle tracking analysis of artificial sputum treated with DTT or H2O2 in the absence of catalase (Figure S1); Non-specific binding of fluorescent IgG antibodies to sputum leukocytes depending on the sample liquefaction degree (Figure S2); Optimized cytometric analysis of sputum leukocytes after enzymatic liquefaction of samples (Figure S3); Impact of hydrogen peroxide on CD11b, HLA-DR and CD63 detection in blood leukocytes (Figure S4). Autofluorescence in DAPI-negative leukocytes from liquefied sputum samples (Table S1).