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Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility of scientific publications. This is even more important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen, and the International Standard ISO 23162:2021. With these tools, we propose that authors should be instructed to follow these laboratory methods in order to publish studies in peer-reviewed journals, preferable by using a checklist as suggested in an Appendix to this article.

Patients with precursor B-cell acute lymphoblastic leukemia (pB-ALL) who have relapsed after allogeneic hematopoietic stem cell transplantation (allo-HSCT), have relapsed more than once, or are resistant upfront have a dismal prognosis. CD19-targeted chimeric antigen receptor (CAR) T cells have evolved as potent immune therapies. Tisagenlecleucel (Tisa-cel) is a commercially available autologous CD19-directed CAR T-cell product. We performed a retrospective study inviting all CAR T-cell centers in Germany to participate. Eighty-one patients with pB-ALL were included. Twenty-eight days after CAR T-cell infusion, 71 patients (87.7%) were in complete response, and 8 (9.9%) were in nonremission. At 2 years, the probabilities of event-free survival (pEFS), relapse-free survival (pRFS), and overall survival (pOS) were 45.3%, 51.7%, and 53.2%, respectively. pEFS was not different in patients without (n = 16, 55.0%) vs with prior allo-HSCT (n = 65, 43.4%). In patients treated after allo-HSCT, the time to relapse after allo-HSCT was a strong predictor of outcome. Patients relapsing within 6 months of allo-HSCT had a disappointing pEFS of 18.4% (pOS = 16.0%); the pEFS for those relapsing later was 55.5% (pOS = 74.8%). Our study provides real-world experience in pediatric, adolescent, and young adult patients with ALL treated with Tisa-cel, where most patients were treated after having relapsed after allo-HSCT. A total of 45.3% were rescued with a single dose of Tisa-cel. Our novel finding that patients with ALL after allo-HSCT had by far a better pEFS if relapse occurred beyond 6 months might be helpful in clinical decision-making and motivates studies to uncover the reasons.

This paper provides the first description of the open-source Glacier Energy and Mass Balance model. GEMB models the ice sheet and glacier surface–atmospheric energy and mass exchange, as well as the firn state. It is a column model (no horizontal communication) of intermediate complexity that includes those processes deemed most relevant to glacier studies. GEMB prioritizes computational efficiency to accommodate the very long (thousands of years) spin-ups necessary for initializing deep firn columns and sensitivity experiments needed to characterize model uncertainty on continental scales. The model is one-way coupled with the atmosphere, which allows the model to be run offline with a diversity of climate forcing but neglects feedback to the atmosphere. GEMB provides numerous parameterization choices for various key processes (e.g., albedo, subsurface shortwave absorption, and compaction), making it well suited for uncertainty quantification and model exploration. The model is evaluated against the current state of the art and in situ observations and is shown to perform well.

International audience; Ice losses from the Greenland and Antarctic ice sheets have accelerated since the 1990s, accounting for a significant increase in the global mean sea level. Here, we present a new 29-year record of ice sheet mass balance from 1992 to 2020 from the Ice Sheet Mass Balance Inter-comparison Exercise (IMBIE). We compare and combine 50 independent estimates of ice sheet mass balance derived from satellite observations of temporal changes in ice sheet flow, in ice sheet volume, and in Earth's gravity field. Between 1992 and 2020, the ice sheets contributed 21.0±1.9 mm to global mean sea level, with the rate of mass loss rising from 105 Gt yr−1 between 1992 and 1996 to 372 Gt yr−1 between 2016 and 2020. In Greenland, the rate of mass loss is 169±9 Gt yr−1 between 1992 and 2020, but there are large inter-annual variations in mass balance, with mass loss ranging from 86 Gt yr−1 in 2017 to 444 Gt yr−1 in 2019 due to large variability in surface mass balance. In Antarctica, ice losses continue to be dominated by mass loss from West Antarctica (82±9 Gt yr−1) and, to a lesser extent, from the Antarctic Peninsula (13±5 Gt yr−1). East Antarctica remains close to a state of balance, with a small gain of 3±15 Gt yr−1, but is the most uncertain component of Antarctica's mass balance. The dataset is publicly available at https://doi.org/10.5285/77B64C55-7166-4A06-9DEF-2E400398E452 (IMBIE Team, 2021).

Individuals seeking out information about human-trafficking and anti-trafficking efforts are increasingly turning to social media as an informational source. However, a lack of traditional informational gatekeeping online has allowed for the rapid proliferation of misinformation via social media. This has been clearly evidenced within the realm of human trafficking by the spread of conspiracy theories instigated by the QAnon-led campaign #SaveTheChildren. Through in-depth interviews with members of the public and professionals involved in anti-trafficking activism we explore how individuals find trustworthy information about human trafficking in light of the public spread of misinformation. Our findings highlight the centrality of distrust as a driving force behind information-seeking on social media. Further, we highlight the tensions that arise from using social media as a primary resource within anti-trafficking education and the limitations of interventions to slow the spread of trafficking-related misinformation. This work provides contextual knowledge for researchers looking to better understand the real-world impacts of misinformation and looking to design better interventions into digital information disorder.

Cet article présente un outil d'analyse élaboré à des fins de formation continue dans le canton de Vaud pour les nouveaux moyens d’enseignement romands de mathématiques. Cet outil, basé sur des textes théoriques et sur le plan d’études romand, recense et articule les principaux apprentissages à réaliser dans les axes thématiques Nombres et Opérations en 3-4H. Nous le présentons puis explicitons de quelle manière il a été utilisé pour analyser les activités de 3-4H concernées proposées sur ESPER. Son usage en formation est également exposé.

Introduction: The aim of this short communication is to examine the journey of scholarship in health professions education (HPE). Methods: We will focus on tangible small steps to start, sustain, and succeed along this journey. Through a proposed innovation in scholarship – micro-scholarship – we will describe how this is similar to and an extension of bite-size or micro-learning, and workplace micro-practice related to just-in-time (JiT) learning settings. Results: We will demonstrate how the small steps for generating and engaging with micro-content can be similarly applied to micro-scholarship. Then, progressive and iterative refinement of output and practice of micro-scholarship can be combined and result in macro-scholarship after cycles of public engagement for final digital or print publication. This stepwise approach creates an accessible, sustainable strategy to achieve success as a scholar in HPE. We will elaborate on micro, macro, and meta matters and celebrate how these small steps encourage and allow broad participation in the creation, critique, and progressive refinement of scholarship. Conclusion: Small, sustainable, steps leads to success as a scholar in Health Professions Education - Micro (macro and meta) matters.

PURPOSE: This study was conducted to investigate whether the use of resorbable bioinductive collagen implant (RBI) in addition to conventional rotator cuff repair (conventional RCR) is cost-effective when compared to conventional RCR alone, in the treatment of full-thickness rotator cuff tears (FT RCT). METHODS: We developed a decision analytic model to compare the expected incremental cost and clinical consequences for a cohort of patients with FT RCT. The probabilities for healing or failure to heal (retear) were estimated from the published literature. Implant and healthcare costs were estimated from a payor’s perspective in 2021 U.S. prices. An additional analysis included indirect cost estimations (e.g., productivity losses). Sensitivity analyses explored the effect of tear size, as well as the impact of risk factors. RESULTS: The base case analysis demonstrated that resorbable bioinductive collagen implant + conventional rotator cuff repair results in incremental costs of $232,468 and an additional 18 healed RCTs per 100 treated patients over 1 year. The estimated incremental cost-effectiveness ratio (ICER) is $13,061/healed RCT compared to conventional RCR alone. When return to work was included in the model, RBI + conventional RCR was found to be cost saving. Cost-effectiveness improved with tear size with the largest benefit seen in massive tears compared to large tears, as well as patients at higher risk of retearing. CONCLUSIONS: This economic analysis demonstrated that RBI + conventional RCR delivered improved healing rates at a marginal increase in costs when compared to conventional RCR alone and is, therefore, cost-effective in this patient population. Considering indirect costs, RBI + conventional RCR resulted in lower costs compared to conventional RCR alone and is, therefore, deemed to be cost saving. LEVEL OF EVIDENCE: Level IV, economic analysis.

Supervised learning as a sub-discipline of machine learning enables the recognition of correlations between input variables (features) and associated outputs (classes) and the application of these to previously unknown data sets. In addition to typical areas of application such as speech and image recognition, fields of applications are also being developed in the sports and fitness sector. The purpose of this work was to implement a workflow for the automated recognition of sports exercises in the Matlab® programming environment and to carry out a comparison of different model structures. First, the acquisition of the sensor signals provided in the local network and their processing were implemented. The functionalities to be realised included the interpolation of lossy time series, the labelling of the activity intervals performed and, in part, the generation of sliding windows with statistical parameters. The preprocessed data were used for the training of classifiers and artificial neural networks (ANN). These were iteratively optimised in their corresponding hyper parameters for the data structure to be learned. The most reliable models were finally trained with an increased data set, validated and compared with regard to the achieved performance. In addition to the usual evaluation metrics such as F1 score and accuracy, the temporal behaviour of the assignments was also displayed graphically, which enabled statements to be made about potential causes for incorrect assignments. In this context, especially the transition areas between the classes were detected as erroneous assignments as well as exercises with insufficient or clearly deviating execution. The best overall accuracy achieved with ANN and the increased dataset was 93.7 %.

Small mammals are an important reservoir for causative agents of numerous infectious diseases, including zoonotic and vector-borne diseases. The occurrence of these pathogens represents a regional but permanent threat for humans and animals in general and might especially weaken military personnel and companion animals in abroad missions. In our study, small mammals collected in military camps in Afghanistan (Feyzabad, Mazar-e Sharif, and Kunduz) were investigated for the presence of apicomplexans using histopathology and molecular methods. For this purpose, well-established and newly developed real-time PCR assays were applied. A high prevalence was detected not only in house mice (Mus musculus), but also in shrews (Crocidura cf. suaveolens) and grey dwarf hamsters (Cricetulus migratorius). The molecular characterization based on the 18S rRNA gene revealed a close relationship to a cluster of Hepatozoon sp. detected in voles of the genus Microtus. Hepatozoon canis DNA was detected in one house mouse as well as in two Rhipicephalus ticks from a dog puppy. In addition, around 5% of the house mice were found to be infected with far related adeleorinids showing the highest sequence identity of 91.5% to Klossiella equi, the only published Klossiella sequence at present. For their better phylogenetic characterization, we conducted metagenomics by sequencing of two selected samples. The resulting 18S rRNA gene sequences have a length of about 2400 base pairs including an insertion of about 500 base pairs and are 100% identical to each other. Histopathology together with organ tropism and detection rates verified this sequence as of Klossiella muris. In conclusion, we documented naturally occurring protozoan stages and the additional taxonomic characterization of a well-known commensal in mice by applying a combination of different approaches. The study is of medical, social, and biological importance for ensuring human and animal health in military camps and also stresses the required awareness for the potential risk of zoonoses.

Abstract: Romantic jealousy describes a feeling when individuals suspect losing their romantic partner to a potential rival. Social media has a high potential to trigger romantic jealousy as it offers almost unlimited opportunities to build social relationships. Thus, it is unsurprising that many studies are now examining social media‐induced jealousy (SoMJ). However, there has been a lack of standardized instruments that can capture SoMJ independently of a specific social media platform. In the present two studies, we developed the 9-item Digital Jealousy Scale (DJS) in German and English and examined its psychometric properties using three heterogeneous samples from Germany and the United Kingdom. The postulated one-factor structure fitted the data very well. Additionally, construct validity was established by showing the expected correlations between the DJS and personality variables such as other jealousy measures, attachment dimensions, self-esteem, and the Big Five dimensions. The DJS is thus a valuable instrument to assess SoMJ.

Chronic right ventricular (RV) pacing can induce left ventricular (LV) dyssynchrony and cause pacemaker induced cardiomyopathy (PiCM). Identifying which patients are at risk for PiCM is limited.Patients receiving RV-only permanent pacemakers (PPMs) at Duke University Medical Center between 2011 and 2017 who had normal baseline ejection fractions (EFs) were identified. Patients who developed a subsequent decrease in EF, died, or underwent cardiac resynchronization therapy, left ventricular assist device, or heart transplant without a competing cause were considered as the primary endpoint. Pre-PPM and post-PPM electrocardiograms (ECGs) were analyzed to extract scalar measurements including the lead one ratio (LOR) as well as advanced-ECG (A-ECG) features to identify predictors of PiCM. Traditional and penalized Cox regression were used to identify variables predictive of the primary endpoint.Pre-PPM ECGs were evaluated for 404 patients of whom 140 (35%) experienced the primary endpoint. Predictors included female sex (hazard ratio [HR] 1.14), a T' wave in V6 (HR 1.31), a P' wave in aVL (HR 0.88), and estimated glomerular filtration rate (HR 0.88). Post-PPM ECGs were evaluated for 228 patients for whom 94 (41%) experienced the primary endpoint. Predictors included female sex (HR 0.50), age (HR 1.06), and a history of congestive heart failure (HR 1.63). Neither LOR nor A-ECG parameters were strong predictors of the primary endpoint.Baseline and paced ECG data provide limited insight into which patients are at high risk for developing PiCM.

High-intensity functional training (HIFT) is a popular activity that combines high effort and compound exercises. Many women aged 20 to 40 who are expected to become pregnant soon are also fond on it. HIFT is an activity in which the heart rate increases significantly, there is an increase in intra-abdominal pressure, free weights are also used. There is poor evidence of HIFT and its effect on pregnancy or childbirth. The subject of this case study was a healthy woman (31 years old) who has long term experience with HIFT. The aim of the study was to analyze training regime (length, intensity, heart rate) and number of steps in the period from the 1st to the 38th week. The effect on the health of the mother and the fetus, the delivery and the birth weight were monitored. The findings show that with an optimally set training program, HIFT can be a safe activity that has no adverse effect on pregnancy, fetal health or childbirth. This is the first such study, so further research is needed.

Open-source environments such as the Computational Fluid Dynamics software OpenFOAM are very appealing for research groups since they allow for an efficient prototyping of new models or concepts. However, for downstream developments to be sustainable, i.e. reproduci-ble and reusable in the long term, a significant amount of maintenance work must be account-ed for. To allow for growth and extensibility, the maintenance work should be underpinned by a high degree of automation for repetitive tasks such as build tests, code deployment and validation runs, in order to keep the focus on scientific work. Here, an information technology environment referred to as OpenFOAM_RCS is presented that aids the centralized mainte-nance of simulation code and setup files for OpenFOAM developments concerned with reac-tor coolant system safety research. It fosters collaborative developments and review processes. State-of-the-art tools for managing software developments are adapted to meet the require-ments of OpenFOAM. A flexible approach for upgrading the underlying installation is pro-posed, based on snapshots of the OpenFOAM development line rather than yearly version releases, to make new functionality available when needed by associated research projects. The process of upgrading within so-called sprint cycles is accompanied by several checks to ensure compatibility of downstream code and simulation setups. Furthermore, the foundation for building a validation data base from contributed simulation setups is laid, creating a basis for continuous quality assurance. The content of this article was initially presented at the 33rd German CFD Network of Com-petence Meeting, held on March 22-23 2022 at GRS in Garching, Germany.

In axial piston pumps, hydrostatically partially balanced contacts are predominantly used. The use of these pumps in variable-speed configurations, such as in electro-hydrostatic axes, has proven to be very challenging in terms of robustness. Particularly affected here is the slipper-swashplate contact, via which the piston loads are supported. The wear processes and driving effects occurring here have not yet been sufficiently addressed in the current state of research. Within the scope of this publication, a model experiment is therefore to be presented which allows the targeted investigation of the wear behaviour of the slipper-swashplate contact under controlled and reproducible conditions. The objectives of the model experiment are, on the one hand, the empirical investigation of the interactions in variable-speed operation and, on the other hand, the validation of simulative considerations.

The extraction of objects from large-scale historical maps has been examined in several studies. With the aim to research urban changes over time, semi-automated and transferable holistic approaches remain to be investigated. We apply a combination of object-based image analysis and vectorization methods on three different historical maps. By further matching and georeferencing an appropriate current geodataset, we provide a concept for analyzing and comparing those valuable sources from the past. With minor adjustments, our end-to-end workflow was transferable to other large-scale maps. The findings revealed that the extraction and spatial assignment of objects, such as buildings or roads, enable the comparison of maps from different times and form a basis for further historical analysis. Performing an affine transformation between the datasets, an absolute offset of no more than 72 m was achieved. The outcomes of this paper, therefore, facilitate the daily work of urban researchers or historians. However, it should be emphasized that specific knowledge is required for the presented subjective methodology.

Glycol nucleic acid (GNA) is an acyclic nucleic acid analog connected via phosphodiester bonds. Crystal structures of RNA–GNA chimeric duplexes indicated that nucleotides of the right-handed (S)-GNA were better accommodated in the right-handed RNA duplex than were the left-handed (R)-isomers. GNA nucleotides adopt a rotated nucleobase orientation within all duplex contexts, pairing with complementary RNA in a reverse Watson–Crick mode, which explains the inabilities of GNA C and G to form strong base pairs with complementary nucleotides. Transposition of the hydrogen bond donor and acceptor pairs using novel (S)-GNA isocytidine and isoguanosine nucleotides resulted in stable base-pairing with the complementary G and C ribonucleotides, respectively. GNA nucleotide or dinucleotide incorporation into an oligonucleotide increased resistance against 3′-exonuclease-mediated degradation. Consistent with the structural observations, small interfering RNAs (siRNAs) modified with (S)-GNA had greater in vitro potencies than identical sequences containing (R)-GNA. (S)-GNA is well tolerated in the seed regions of antisense and sense strands of a GalNAc-conjugated siRNA in vitro. The siRNAs containing a GNA base pair in the seed region had in vivo potency when subcutaneously injected into mice. Importantly, seed pairing destabilization resulting from a single GNA nucleotide at position 7 of the antisense strand mitigated RNAi-mediated off-target effects in a rodent model. Two GNA-modified siRNAs have shown an improved safety profile in humans compared with their non-GNA-modified counterparts, and several additional siRNAs containing the GNA modification are currently in clinical development.

Purpose Positron emission tomography (PET) with O-(2-[18F]fluoroethyl)-L-tyrosine ([18F]FET) is a well-established tool for non-invasive assessment of adult central nervous system (CNS) tumors. However, data on its diagnostic utility and impact on clinical management in children and adolescents are limited. Methods Twenty-one children and young adults (13 males; mean age, 8.6 ± 5.2 years; range, 1–19 at initial diagnosis) with either newly diagnosed (n = 5) or pretreated (n = 16) CNS tumors were retrospectively analyzed. All patients had previously undergone neuro-oncological work-up including cranial magnetic resonance imaging. In all cases, [18F]FET-PET was indicated in a multidisciplinary team conference. The impact of PET imaging on clinical decision-making was assessed. Histopathology (n = 12) and/or clinical and imaging follow-up (n = 9) served as the standard of reference. Results The addition of [18F]FET-PET to the available information had an impact on further patient management in 14 out of 21 subjects, with avoidance of invasive surgery or biopsy in four patients, biopsy guidance in four patients, change of further treatment in another five patients, and confirmation of diagnosis in one patient. Conclusion [18F]FET-PET may provide important additional information for treatment guidance in pediatric and adolescent patients with CNS tumors.

Das unter eidgenössischem Denkmalschutz stehende Ritterhaus Bubikon blickt im Zürcher Oberland auf rund 800 Jahre Geschichte zurück. Im Rahmen einer Führung wird den Museumsbesuchern seit Frühjahr 2022, nach einer knapp drei Jahre andauernden Planungs- und Restaurierungsphase, der Zugang in das historische Dachwerk ermöglicht. Diese Metadaten wurden zur Verfügung gestellt von der Literaturdatenbank RSWB®plus

Haemorrhagic shock remains a leading preventable cause of death amongst trauma patients. Failure to identify retroperitoneal haemorrhage (RPH) can lead to irreversible haemorrhagic shock. The arteries of the middle retroperitoneal region (i.e., the 1st to 4th lumbar arteries) are complicit in haemorrhage into the retroperitoneal space. However, predictive injury patterns and subsequent management implications of haemorrhage secondary to bleeding of these arteries is lacking.We performed a retrospective cohort study of patients diagnosed with retroperitoneal haemorrhage who presented to our Level-1 Trauma Centre (2009-2019). We described the associated injuries, management and outcomes relating to haemorrhage of lumbar arteries (L1-4) from this cohort to assess risk and management priorities in non-cavitary haemorrhage compared to RPH due to other causes.Haemorrhage of the lumbar arteries (LA) is associated with a higher proportion of lumbar transverse process (TP) fractures. Bleeding from branches of these vessels is associated with lower systolic blood pressure, increased incidence of massive transfusion, higher shock index, and a higher Injury Severity Score (ISS). A higher proportion of patients in the LA group underwent angioembolisation when compared to other causes of RPH.This study highlights the injury patterns, particularly TP fractures, in the prediction, early detection and management of haemorrhage from the lumbar arteries (L1-4). Compared to other causes of RPH, bleeding of the LA responds to early, aggressive haemorrhage control through angioembolisation. These injuries are likely best treated in Level-1 or Level-2 trauma facilities that are equipped with angioembolisation facilities or hybrid theatres to facilitate early identification and management of thoracolumbar bleeds.

International audience; Based on a monograph of the Association Baptiste pour l’Entraide et la Jeunesse (Abej), an association based in Lille that was active early on in dealing with homelessness, this article analyses the processes that drove the professionalisation of care for homeless people in France. Having started in a Baptist community, the Abej soon became a key player bringing aid to the excluded. It developed through a series of changes that affected the non-profit sector, health and social care policies, and anti-poverty movements since the mid-1970s. This development coincided with the formation of a professionalised care system for the homeless, so that the case of Abej provides unique insight into the processes involved. Lastly, the article shows the reconfiguration of the association’s identity: its religious identity, while not disappearing, faded away in favour of a growing recognition of its professionalism.; À partir d’une monographie de l’Association baptiste pour l’entraide et la jeunesse (Abej), association lilloise précocement investie dans le traitement de la « question SDF », cet article analyse les processus qui ont nourri la professionnalisation de la prise en charge des personnes sans domicile en France. Issue d’une communauté de vie baptiste, l’Abej s’impose rapidement comme un acteur de référence en matière de traitement des situations d’exclusion. Son développement s’opère à travers un ensemble de changements qui affectent le milieu associatif, le secteur médico-social, et celui de la lutte contre la pauvreté à partir du milieu des années 1970. Ce développement est concomitant de la formation d’un système de prise en charge dédié aux personnes sans domicile, de sorte que le cas de l’Abej fournit un éclairage original des processus qui y ont participé. L’article montre enfin la reconfiguration identitaire de l’association : sans que son identité religieuse ne disparaisse, elle s’estompe au profit d’une reconnaissance croissante de son professionnalisme.

The Euler-Euler model is widely used in bubbly flow simulations up to industrial dimensions. The standard Euler-Euler model is based on the phase-averaging method. After averaging, the bubble forces in the field equations are functions of the local gas volume fraction. In simulations, when the bubble diameter is larger than the computational cell spacing, the forces can transport the gas belonging to the same bubble in different directions. By contrast, a closure model for the bubble force is typically developed based on the assumption that the force is a resultant force that acts on the bubble's center-of-mass. This inconsistency can lead to a nonphysical gas concentration in the center of a channel or near the channel wall if the bubble diameter is larger than the cell spacing. The purpose of the present contribution is to developed an Euler-Euler model where the bubble force consistency is recovered for two-phase flow simulations where the diameter of the disperse phase can be larger than the cell spacing. Such an Euler-Euler model is developed by combining an existing particle-center-averaged Euler-Euler framework with a Gaussian convolution method. To validate this Euler-Euler approach, a comparison is made with experimental data for the bubbly flows in two different vertical pipes. The results show that the proposed Euler-Euler model recovers the bubble force consistency and alleviates the over-prediction of the gas volume fraction peak near the wall, while its simulation results in the axial gas and liquid velocity and the liquid turbulence kinetic energy are similar to the results of the standard Euler-Euler model.

Artículo escrito por un elevado número de autores, solo se referencian el que aparece en primer lugar, los autores pertenecientes a la UAM y el nombre del grupo de colaboración, si lo hubiere, We describe the Milky Way Survey (MWS) that will be undertaken with the Dark Energy Spectroscopic Instrument (DESI) on the Mayall 4 m telescope at the Kitt Peak National Observatory. Over the next 5 yr DESI MWS will observe approximately seven million stars at Galactic latitudes ∣b∣ > 20°, with an inclusive target selection scheme focused on the thick disk and stellar halo. MWS will also include several high-completeness samples of rare stellar types, including white dwarfs, low-mass stars within 100 pc of the Sun, and horizontal branch stars. We summarize the potential of DESI to advance understanding of the Galactic structure and stellar evolution. We introduce the final definitions of the main MWS target classes and estimate the number of stars in each class that will be observed. We describe our pipelines for deriving radial velocities, atmospheric parameters, and chemical abundances. We use ≃500,000 spectra of unique stellar targets from the DESI Survey Validation program (SV) to demonstrate that our pipelines can measure radial velocities to ≃1 km s−1 and [Fe/H] accurate to ≃0.2 dex for typical stars in our main sample. We find the stellar parameter distributions from ≈100 deg2 of SV observations with ≳90% completeness on our main sample are in good agreement with expectations from mock catalogs and previous surveys, This equipment was funded by MoE, NSTC, and National Tsing Hua University. C.A.P. acknowledges financial support from the Spanish Ministry of Science and Innovation (MICINN) projects AYA2017-86389-P and PID2020-117493GB-I00. C.J.M. acknowledges financial support from Imperial College London through an Imperial College Research Fellowship grant. M.V. and L.B.e.S. acknowledge support from NASA-ATP award 80NSSC20K0509. The work of A.D. and J.N. is supported by NOIRLab, which is managed by the Association of Universities for Research in Astronomy (AURA) under a cooperative agreement with the National Science Foundation. T.S.L. acknowledges financial support from the Natural Sciences and Engineering Research Council of Canada (NSERC) through grant RGPIN-2022-04794. B.T. G. was supported by grant ST/T000406/1 from the Science and Technology Facilities Council (STFC). This project has received funding from the European Research Council under the European Unionʼs Horizon 2020 research and innovation program (grant agreement No. 101020057). This research made use of computing time available on the high-performance computing system at the Instituto de Astrofisica de Canarias

Full list of authors: Myers, Adam D.; Moustakas, John; Bailey, Stephen; Weaver, Benjamin A.; Cooper, Andrew P.; Forero-Romero, Jaime E.; Abolfathi, Bela; Alexander, David M.; Brooks, David; Chaussidon, Edmond; Chuang, Chia-Hsun; Dawson, Kyle; Dey, Arjun; Dey, Biprateep; Dhungana, Govinda; Doel, Peter; Fanning, Kevin; Gaztanaga, Enrique; Gontcho, Satya Gontcho; Gonzalez-Morales, Alma X.; Hahn, ChangHoon; Herrera-Alcantar, Hiram K.; Honscheid, Klaus; Ishak, Mustapha; Karim, Tanveer; Kirkby, David; Kisner, Theodore; Koposov, Sergey E.; Kremin, Anthony; Lan, Ting-Wen; Landriau, Martin; Lang, Dustin; Levi, Michael E.; Magneville, Christophe; Napolitano, Lucas; Martini, Paul; Meisner, Aaron; Newman, Jeffrey A.; Palanque-Delabrouille, Nathalie; Percival, Will; Poppett, Claire; Prada, Francisco; Raichoor, Anand; Ross, Ashley J.; Schlafly, Edward F.; Schlegel, David; Schubnell, Michael; Tan, Ting; Tarle, Gregory; Wilson, Michael J.; Yeche, Christophe; Zhou, Rongpu; Zhou, Zhimin; Zou, Hu.--This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited., In 2021 May, the Dark Energy Spectroscopic Instrument (DESI) began a 5 yr survey of approximately 50 million total extragalactic and Galactic targets. The primary DESI dark-time targets are emission line galaxies, luminous red galaxies, and quasars. In bright time, DESI will focus on two surveys known as the Bright Galaxy Survey and the Milky Way Survey. DESI also observes a selection of "secondary" targets for bespoke science goals. This paper gives an overview of the publicly available pipeline (desitarget) used to process targets for DESI observations. Highlights include details of the different DESI survey targeting phases, the targeting ID (TARGETID) used to define unique targets, the bitmasks used to indicate a particular type of target, the data model and structure of DESI targeting files, and examples of how to access and use the desitarget code base. This paper will also describe "supporting" DESI target classes, such as standard stars, sky locations, and random catalogs that mimic the angular selection function of DESI targets. The DESI target-selection pipeline is complex and sizable; this paper attempts to summarize the most salient information required to understand and work with DESI targeting data. © 2023. The Author(s). Published by the American Astronomical Society., A.D.M. and J.M. were supported by the U.S. Department of Energy, Office of Science, Office of High Energy Physics, under Award Numbers DE-SC0019022 and DE-SC0020086. A.P.C. is supported by a Taiwan Ministry of Education Yushan Fellowship and Taiwan Ministry of Science and Technology grant No. 109-2112-M-007-011-MY3. This research is supported by the Director, Office of Science, Office of High Energy Physics of the U.S. Department of Energy under Contract No. DEAC0205CH11231, and by the National Energy Research Scientific Computing Center, a DOE Office of Science User Facility under the same contract; additional support for DESI is provided by the U.S. National Science Foundation, Division of Astronomical Sciences under Contract No. AST-0950945 to the NSF's National Optical-Infrared Astronomy Research Laboratory; the Science and Technologies Facilities Council of the United Kingdom; the Gordon and Betty Moore Foundation; the Heising-Simons Foundation; the French Alternative Energies and Atomic Energy Commission (CEA); the National Council of Science and Technology of Mexico (CONACYT); the Ministry of Science and Innovation of Spain (MICINN), and by the DESI Member Institutions: https://www.desi.lbl.gov/collaborating-institutions., With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001131-S).

Decreasing the activation of pathology-activated microglia is crucial to prevent chronic inflammation and tissue scarring. In this study, we used a stab wound injury model in zebrafish and identified an injury-induced microglial state characterized by the accumulation of lipid droplets and TAR DNA-binding protein of 43 kDa (TDP-43)+ condensates. Granulin-mediated clearance of both lipid droplets and TDP-43+ condensates was necessary and sufficient to promote the return of microglia back to the basal state and achieve scarless regeneration. Moreover, in postmortem cortical brain tissues from patients with traumatic brain injury, the extent of microglial activation correlated with the accumulation of lipid droplets and TDP-43+ condensates. Together, our results reveal a mechanism required for restoring microglia to a nonactivated state after injury, which has potential for new therapeutic applications in humans.

Excessive sound, vibration, and light are detrimental to rodent welfare, yet these parameters are rarely recorded in vivaria. Whether housing environments exceed the suggested thresholds and which specific factors may alter these parameters is generally unknown. The goal of this study was to determine how environmental factors may alter sound, vibration, and light at the room and cage levels. Measurements were made using an ultrasonic microphone, accelerometer, and light sensor. Measurement sites were 1) in open air at a central location in 64 rooms located in 9 buildings, and 2) inside an empty mouse or rat cage containing chow, water, and bedding and located on an animal transfer station (n = 51) or housing rack (n = 102). Information collected for each transfer station and rack measurement included the year of manufacture, the species on the rack, and the number of cages on the rack. For each location, a baseline measurement was taken with the transfer station turned off, followed by another measurement after the transfer station was turned on. In general, many factors influenced ambient sound, vibration, and light, indicating that values are not uniform across rodent rooms in the same institution or across cages in a single room. Sound peaks capable of startling rodents were measured in association with hallway ultrasonic motion sensors and during cage change. Vibration and light intensity were generally low when cages were located on the rack. In contrast, active transfer stations had more vibration and light intensity, reaching levels that were potentially stressful for rodents. These data reflect the ambient sound, vibration, and light that rodents experience during normal facility operations. These patterns may extend to other locations, but given the variability in all parameters, the data highlight the need for institutions to conduct their own monitoring.