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Additional file 2. Fig. 2. Scatterplot, showing ‘pegRNA Score’ vs. editing efficiency of reported pegRNAs. Reported pegRNAs from Anzalone et al. Fig. 4a with their respective average editing efficiency plotted against their pegRNA Score as calculated by PnB Designer. A linear regression was fitted to investigate the relationship between these two variables and a R2 value of 0.297 was obtained. Figure was made using MS Excel (2016).

Additional file 1. Fig. 1. Example output tables for good and suboptimal guide design for PE and BE. a Example output table for prime editing, showing pegRNA design with a high ‘pegRNA Score’ and no additional penalties (row 1), as well as pegRNAs suffering from design flaws and consequently assigned with a low ‘pegRNA Score’ (row 5 and 6). b Example output table for base editing. Three different CBEs can target this SNV. However, two of those show bystander bases in the editing window (marked in blue), which could be edited as well (row 1 and 2). Therefore, the use of Target-AID (row 3) should be prioritized for ‘clean’ editing at this locus.