1. The differential activation of intracellular signaling pathways confers the permissiveness of embryonic stem cell derivation from different mouse strains
- Author
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Hitoshi Niwa and Satoshi Ohtsuka
- Subjects
STAT3 Transcription Factor ,Permissiveness ,MAPK/ERK pathway ,endocrine system ,Signal responsiveness ,Real-Time Polymerase Chain Reaction ,Leukemia Inhibitory Factor ,Mice ,Species Specificity ,Animals ,STAT3 ,Molecular Biology ,Embryonic Stem Cells ,reproductive and urinary physiology ,DNA Primers ,Stat3 ,biology ,Janus kinase 1 ,urogenital system ,LIF signaling ,Gene Expression Regulation, Developmental ,Janus Kinase 1 ,Stem Cells and Regeneration ,Embryonic stem cell ,Molecular biology ,Mice, Mutant Strains ,Mitogen-activated protein kinase ,embryonic structures ,biology.protein ,MAP kinase ,biological phenomena, cell phenomena, and immunity ,Signal transduction ,Leukemia inhibitory factor ,Signal Transduction ,Developmental Biology - Abstract
The requirement of leukemia inhibitory factor (LIF) for the establishment and maintenance of mouse embryonic stem cells (ESCs) depends on the genetic background of the ESC origin. To reveal the molecular basis of the strain-dependent function of LIF, we compared the activation of the intracellular signaling pathways downstream of LIF in ESCs with different genetic backgrounds. We found that the JAK-Stat3 pathway was dominantly activated in ESCs derived from ‘permissive’ mouse strains (129Sv and C57BL6), whereas the MAP kinase pathway was hyperactivated in ESCs from ‘non-permissive’ strains (NOD, CBA and FVB). Artificial activation of Stat3 supported stable self-renewal of ESCs from non-permissive strains. These data suggest that the difference in the balance between the two intracellular signaling pathways underlies the differential response to LIF., Highlighted article: Different mouse strains show distinct JAK-Stat and MAPK activity responses to LIF treatment, accounting for the varying ease of generating stem cells from these lines.
- Published
- 2015
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