1. Antilipase activity guided fractionation of Vinca major
- Author
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Singh, Sukhdev, Kanwar, Singh Shamsher, and Kumari, Indu
- Subjects
0301 basic medicine ,Multidisciplinary ,Chromatography ,biology ,Chemistry ,Active site ,030209 endocrinology & metabolism ,Lipid metabolism ,Fractionation ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Column chromatography ,Biochemistry ,Docking (molecular) ,Sephadex ,biology.protein ,medicine ,Akuammine ,Lipase ,General ,lcsh:Science (General) ,lcsh:Q1-390 ,medicine.drug - Abstract
Methanolic extract of flowers of Vinca major (VFE) reduced the activity of porcine pancreatic lipase (PPL) which was reported in our previous study. To identify the antilipase molecule(s) in the VFE, the antilipase activity-guided fractionation for the purification of lipase inhibitory molecule(s) by Silica Gel and Sephadex G-50 column chromatography was attempted, and the final anti-lipase fraction was examined by ESI-MS and FT-IR spectroscopy. Two molecules namely, Majoridine and Akuammine were elucidated to be present in the flowers of V. major which possessed high PPL inhibition activity. Further, both molecules were looked in silico for their interactions with PPL and other important proteins of lipid metabolism using molecular docking studies. Majoridine and Akuammine demonstrated significant binding interactions with active site residues of PPL. Keywords: Vinca major, Fractionation, ESI-MS, FT-IR, Majoridine, Akuammine, Docking
- Published
- 2018