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Objective: Osteoarthritis (OA) is the most common chronic degenerative joint disease, which represents the leading cause of age-related disability. Here, this study aimed to depict the intercellular heterogeneity of OA synovial tissues.Methods: Single-cell RNA sequencing (scRNA-seq) data were preprocessed and quality controlled by the Seurat package. Cell cluster was presented and cell types were annotated based on the mRNA expression of corresponding marker genes by the SingleR package. Cell-cell communication was assessed among different cell types. After integrating the GSE55235 and GSE55457 datasets, differentially expressed genes were identified between OA and normal synovial tissues. Then, differentially expressed marker genes were overlapped and their biological functions were analyzed.Results: Totally, five immune cell subpopulations were annotated in OA synovial tissues including macrophages, dendritic cells, T cells, monocytes and B cells. Pseudo-time analysis revealed the underlying evolution process in the inflammatory microenvironment of OA synovial tissue. There was close crosstalk between five cell types according to the ligand-receptor network. The genetic heterogeneity was investigated between OA and normal synovial tissues. Furthermore, functional annotation analysis showed the intercellular heterogeneity across immune cells in OA synovial tissues.Conclusion: This study offered insights into the heterogeneity of OA, which provided in-depth understanding of the transcriptomic diversities within synovial tissue. This transcriptional heterogeneity may improve our understanding on OA pathogenesis and provide potential molecular therapeutic targets for OA.

Additional file 1: eTable 1. Baseline sample characteristics between eligible and ineligible participants. eTable 2. Frequencies of responses on the MARS among the suboptimal adherence group (n=234). eFigure 1. The distribution of suboptimal adherence in the low, medium and high resilience group.

Missile Datcom (hereinafter referred to as Datcom) is an aerodynamic engineering calculation software developed by the US Air Force Research Laboratory. It is commonly used in the preliminary design of the shape scheme of missiles, aircraft and other aircraft. In this paper, Datcom is used to calculate the aerodynamic parameters of a two-dimensional ballistic correction projectile(D2BCP), and the calculated data is compared with the wind tunnel test data. The analysis results show that Datcom calculates the axial force coefficient (CA) of the projectile with large system deviation, however, the deviation can be eliminated by a certain compensation, thereby obtaining a more accurate CA of the projectile. Datcom calculates the normal force coefficient (CN) with high Mach number accuracy, but there is an unacceptable deviation of the projectile CN calculated at small angle of attack ( α ). The calculation of pitching moment coefficient with α (CMa) is higher, but the error is larger under individual Mach numbers.

Long-wall mining method has its own advantages, such as small mining-cutting ratio, the simple technique of recovery and high level of mechanization, which is the optimal method used to mine gently inclined and extremely thin iron mine. How to ensure the safety of workers and equipment during mining is the key technical process. Based on elastic–plastic mechanics theory, the mechanic model of long-wall method mining gently inclined and extremely thin iron mine is established and the critical safety distance of workface is obtained. With the FLAC-2D simulation software, the rule of stress change, the deformation of workface and surrounding rock in different control-roof width are also summarized. The final results accord with the ones of theory test. We confirm the support-frame resistance of workface by analogy-statistic method. The results prove that hydraulic support-frame have the ability to provide enough pressure to assure the stability of working face within the 5-m of control-roof width.

Targeted at the orebody section from Guandian Mining to Dazhuang Mining between line -8’ and -16’, the study focuses on the mechanism of wall rock deformation and failure and the deformation of ground surface led by the mining of stratified ore bodies with the long wall mining. FLAC software is adopted to analyze and study the simulation of the stress change of quarry wall rock without support on the working surface. Conclusion is drawn on the regularity for stress change of quarry wall rock to provide scientific and rational guide for the mining engineering practice.

The key of the stability numerical simulation in cavity is to grasp its 3D configuration and spatial location accurately. Based on the actual situation of Shi Rengou iron mine and cavity 3D laser detection system, this paper develops the cavity 3D model and geological 3D model by using the 3Dmine software. Numerical computation model is developed by the coupling of 3Dmine and Flac3D with the data conversion of coupled mode. Numerical analog computation is conducted, and the surrounding rock stability in cavity is analyzed according to the simulation results. The research shows that the combination of the advantages of CMS, 3Dmine and Flac3D provides a new and effective way for numerical simulation research on the stability of rock engineering.

In order to study ore mining process' influence on rock mechanics and stability of mine, Stope structure parameters' influence on surrounding rock mechanics and stability, and the overlying rock and ground surface deformation mobile rule. Simulating the mining sequence by 3D software FLAC, Through the analysis of the maximum principal stress, Z direction stress and displacement of t the Z direction of different mining sequence, the optimal scheme is selected, and the results can provide reference for the safe production in mines.

All tailings filling technology that take full use of tailings for filling the whole aggregate has been widely used at home and abroad, which can not only take the advantages of increased ore recovery, but also reduce the emission of pollutants and protect the environment. In this paper, based on the background of Jinshandian iron mine, mainly through the experimental grain size analysis, sedimentation test, slump test, the viscosity test, uniaxial compressive strength test and scanning electron microscopy, the nature of the whole tailings, flow properties of filling cement slurry and the compressive strength of various cement tailings specimen were tested and analyzed. The optimal mix of all tailings filling material of Jinshandian Iron mine is confirmed which provides a reliable basis to the smooth implementation of cemented filling technology in Jinshandian iron mine.

Close-vertical underpass existing subway is the hardest condition during civil subway construction. Taking Beijing Metro Line 10 vertical Underpass Metro line 1 as the project background, the paper focus on the interacting of the new building and existing tunnel during the whole construction process. Adopting Ansys and Flac-3d to build large-scale mathematical and physical artificial models, by the analysis of the new building and existing tunnel deformation and stress alteration we got the Mechanical Characteristic of the new and existing subway tunnel. The research indicates that the main affect range of the new tunnel construction to the old tunnel is mainly in double excavation scope (6m). The main characterizations of the existing tunnel include the liner stress decreased, incline and recover. The existing of metro line 1 takes block conductive effect to surrounding rock stress and deformation caused by excavation.

Aimed to dissolve the question that it is difficult to maintain the developing entry during mining in the weak and fragmentized ore body, carried out the site monitoring, convergence monitoring and numerical simulation to disclose the distortion mechanism of developing entry of chengchao Iron Mine. the data provide the support for the preparatory workings deformation mechanism. the result of the monitoring and numerical simulation indicated that the displacement of preparatory workings is relative to mining course, and the displacement increase with the depth increase. The result is to provide a theoretical support to choose the system of protecting synthetically in deep mines and the right time of support..

The new rubber planting suitability evaluation model based on the cloud theory, rough set and fuzzy neural network has been put forward according to the lack of influencing factors samples on rubber growth. Qualitative description of the rubber planting rules can be converted into quantitative rubber planting influencing factors sample data by the forward cloud generator of the evaluation model. Moreover, rough set is used to reduce the sample data. Then, the membership of each evaluation element is obtained via the fuzzy neural network. Finally, the evaluation grade is calculated though evaluation element membership. The preliminary research indicates that the model, which combines spatial clustering analysis, can scientifically and fleetly divide the study area into the most suitable area, suitable area, the less suitable area and unsuitable area.

The terminal-guidance shell is a shell which can be launched by the conventional cannon, but precisely guided during the terminal part of the trajectory. It can attack the static or moving slowly target accurately in the intermediate range and long range. It attacks the target on the top with big fall angle. It has the characters as launched frequently, high initial speed, continuous emission, used flexibly, repair easily as the common shell, also has the characters the common shell doesn't have , such as high accuracy and great power. However, its trajectory characters become complex, so it is a hard task to research its ballistics and motion rules. Typical characteristic of a certain terminal-guidance shell is: not-control shell's rotating velocity and keeping certain but not-unchangeable rotating velocity. It is mainly discussed in this article.

Aimed to the character of high speed of bullet, combined with the principle of linear CCD camera, the software for data acquisition and processing is developed in this article based on the computer programming techniques and image processing techniques. It is introduced integrated design scheme and realization of software for measurement of bullet attitude in Windows by VC++ and MATLAB, which solves the problem that bullet attitude is not measured at real-time. Application of experiment for measuring bullet attitude, this system can accomplish the task of measuring bullet's angle of attack and velocity parameters at real-time.