Search

Paecilomyces clematidis Spetik, Eichmeier, Gramaje & Berraf-Tebbal, sp. nov. (Fig. 2) Mycobank number: MB843540 Type: CZECH REPUBLIC, Breclav: Lednice, university garden (48°47’33.3”N, 16°47’55.7”E), isolated from the root of Clematis ‘Snow Queen’ (Ranunculaceae), May 2021, M. Spetik, Holotype: BRNU 677844, ex-type living culture CBS 148466 = MEND-F-0560. Sexual morph: not observed. Asexual morph: Hyphomycetous. Conidiophores hyaline, septate, irregularly branched. Phialides with a cylindrical basal portion tapering to a thin long neck, solitary directly on vegetative hyphae or in groups of two–four per metula. Conidia (2.5–)3.3–3.6(–4.2) × (1.7–)2.2–2.5(–3.2) µm, mean ± SD 3.4 ± 0.4 × 2.4 ± 0.4 µm hyaline, ellipsoidal with flattened base, pyriform, produced in chains. Chlamydospores usually produced laterally or terminally on short side branches of superficial or immersed hyphae, thick-walled, initially smooth later becoming rough and warted, subglobose to globose, dark green, 4.5–6.2 µm diam. Culture characteristics. On PDA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous, almost no growth at 18 ° C;> 90 mm diam at 25°, 30° and 35 ° C; 42 mm diam at 40 ° C; no growth at 45 ° C. On MEA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 ° C. On OA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 ° C. On CYA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 ° C. Etymology: —refers to the host genus (Clematis). Additional specimens examined: CZECH REPUBLIC, Breclav: Lednice, university garden (48°47’33.4”N, 16°47’55.7”E), isolated from the root of Clematis ‘ Snow Queen’ (Ranunculaceae), May 2021, M. Spetik, living culture MEND-F-0561 .

Paecilomyces clematidis Spetik, Eichmeier, Gramaje & Berraf-Tebbal, sp. nov. (Fig. 2) Mycobank number: MB843540 Type: CZECH REPUBLIC, Breclav: Lednice, university garden (48°47’33.3”N, 16°47’55.7”E), isolated from the root of Clematis ‘Snow Queen’ (Ranunculaceae), May 2021, M. Spetik, Holotype: BRNU 677844, ex-type living culture CBS 148466 = MEND-F-0560. Sexual morph: not observed. Asexual morph: Hyphomycetous. Conidiophores hyaline, septate, irregularly branched. Phialides with a cylindrical basal portion tapering to a thin long neck, solitary directly on vegetative hyphae or in groups of two–four per metula. Conidia (2.5–)3.3–3.6(–4.2) × (1.7–)2.2–2.5(–3.2) µm, mean ± SD 3.4 ± 0.4 × 2.4 ± 0.4 µm hyaline, ellipsoidal with flattened base, pyriform, produced in chains. Chlamydospores usually produced laterally or terminally on short side branches of superficial or immersed hyphae, thick-walled, initially smooth later becoming rough and warted, subglobose to globose, dark green, 4.5–6.2 µm diam. Culture characteristics. On PDA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous, almost no growth at 18 ° C;> 90 mm diam at 25°, 30° and 35 ° C; 42 mm diam at 40 ° C; no growth at 45 ° C. On MEA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 ° C. On OA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 ° C. On CYA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 ° C. Etymology: —refers to the host genus (Clematis). Additional specimens examined: CZECH REPUBLIC, Breclav: Lednice, university garden (48°47’33.4”N, 16°47’55.7”E), isolated from the root of Clematis ‘ Snow Queen’ (Ranunculaceae), May 2021, M. Spetik, living culture MEND-F-0561 ., Published as part of Spetik, Milan, Berraf-Tebbal, Akila, Gramaje, David, Mahamedi, Alla Eddine, Stusková, Katerina, Burgova, Jana & Eichmeier, Ales, 2022, Paecilomyces clematidis (Eurotiales, Thermoascaceae): a new species from Clematis root, pp. 238-246 in Phytotaxa 559 (3) on pages 242-243, DOI: 10.11646/phytotaxa.559.3.2, http://zenodo.org/record/7021706

Timing of pruning may affect wound susceptibility to wood-invading fungi, such as those associated with grapevine trunk diseases (GTDs). This study aimed to determine the effect of pruning time on natural fungal infection in six vineyards in Galicia, Spain, during two periods of three months each, from November to February and from February to May by ITS2 high-throughput amplicon sequencing. Fungal microbiome composition did not differ significantly with year of sampling. Pruned canes harboured a core community of fungal species, which appeared to be independent of the infection period. Accumulated rainfall over 8 and 11 weeks after pruning positively correlated with the total fungal microbiome. A seasonal effect on GTD fungal infection was detected for most genera, with a higher percent detected after pruning in February (winter) as compared with that of pruning in November (mid-autumn). Early pruning is recommended to reduce the infections caused by GTD fungi in Galicia., Funding was provided by Ministerstvo Školství, Mládeže a Tělovýchovy (CZ.02.1.01./0.0/0.0/16_017/0002334).