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© 2017 Elsevier B.V. The swelling prediction of bentonite–sand mixtures due to wetting is very important in evaluating the long term performance of the engineered barrier in the high level radioactive waste disposal system and the hydraulic barriers in geoenvironmental constructions. Sun et al. (2015) proposed the swelling prediction model of bentonite-sand mixtures due to full hydration, and predicted the swelling of different types of bentonite-sand mixtures, which was verified, to be consistent with the swelling test results. However, the predicted swelling results of bentonite-sand mixtures with extremely high sand content obtained by the original swelling prediction model have a large deviation from the test results. The reason is that the original model is based on an assumption that all the pores and available water are only associated with bentonite/montmorillonite fraction. However, for mixtures with extremely high sand content, the sand skeleton exists and resists the external stress from the very beginning. At the same time, the bentonite, dispersing in the sand skeleton, in contact with the pore fluid, swells freely to fully saturated state, however, still fails to fill the sand skeleton voids completely. In this research, the original swelling prediction model is extended by introducing the concept of critical filling sand content and critical contact stress. When sand content is more than the critical filling sand content, the deformation of the mixture is mainly due to the sand skeleton deformation. After the stress increases greater than the critical contact stress, the saturated bentonite fills the sand skeleton voids completely, and the swelling can still be predicted by the original swelling prediction model. In the extended model, the swelling prediction can be divided into three zones according to the two limit values of critical sand content and critical filling sand content. In different zones, the distribution ratio of the vertical stress that is borne by montmorillonite and sand skeleton is different. The extended swelling prediction model is applicable to predict the swelling due to full hydration for mixtures within the full range of sand content, and is verified by the swelling tests on bentonite–sand mixtures with various sand contents.

Increased expression of STMN1 has been observed in many tumor forms, but its expression and potential biological role in pancreatic cancer is still unknown. In this study, we demonstrated that STMN1 was expressed to a large extent in pancreatic cancer tissues and cell lines as compared to normal pancreatic tissues. Suppression of STMN1 expression via transfection with STMN1-specific siRNA could not only significantly inhibit the proliferation, migration and invasion ability of Panc-1 cells, but also enhance the apoptosis of Panc-1 cells. In addition, downregulation of STMN1 obviously enhanced the acetylation level of α-tubulin. All these results indicated that STMN1 plays an important role in pancreatic cancer development, and might serve as a potential therapeutic target for pancreatic cancer.

© 2016 The Authors. It is particularly necessary to study the deformation, strength and the changes of pore water pressure of bentonite-based buffer/backfill materials under the undrained condition. A series of isotropic compression tests and triaxial shear tests under undrained conditions were conducted on the compacted saturated/unsaturated GMZ bentonite-sand mixtures with dry mass ratio of bentonite/sand of 30:70. During the tests, the images of the sample were collected by photographic equipment and subsequently were cropped, binarized and centroids marked by image processing technique. Based on identification of the variation of the position of marked centroids, the deformation of the sample can be determined automatically in real-time. Finally, the hydro-mechanical behaviour of saturated and unsaturated bentonite-sand mixtures under the undrained condition can be obtained. From results of triaxial shear tests on unsaturated samples under constant water content, inflated volumetric deformation transforms to contractive volumetric deformation due to the increase of the confining pressure and lateral expansion deformation are observed due to the increase in the shearing stress. Moreover, the net mean stress affects the initial stiffness, undrained shear strength and deformation of the sample during the undrained shear tests.

© 2015 Elsevier B.V. The swelling characteristic of bentonite-sand mixtures is an important index in evaluating the long term performance of the deep geological repository of nuclear waste. In this study the swelling characteristics of different types of bentonite-sand mixtures with various sand contents are compared. It is concluded that for pure bentonite and bentonite-sand mixtures with less sand than the critical sand content, the relationship between montmorillonite void ratio and vertical stress can be expressed as a unique line in a double logarithmic plot, and volumetric strain under a given vertical stress and swelling pressure at a constant volume after full saturation can be predicted based on this line; however, for mixtures with more sand than the critical sand content, the above relationship deviates from the line when the stress is larger than the starting deviation stress, which can be determined using the sand skeleton void ratio. Before the sand skeleton forms, the vertical stress is borne mainly by montmorillonite particles and the amount of swelling at full saturation is determined by the content of montmorillonite per unit volume, however, after the sand skeleton forms the vertical stress is borne by both the montmorillonite and the sand skeleton. The stress distribution coefficient is proposed to present a proportion of the vertical stress that is borne by both parts. A predictive method, verified by the swelling tests on bentonite-sand mixtures with various sand contents, is suggested to predict the amount of swelling due to saturation in the full range of sand content.

Liver X receptor α (LXRα) and sterol regulatory element binding protein-1c (SREBP-1c) were studied in rats with non-alcoholic steatohepatitis (NASH) induced by a high-fat diet. Forty 5-week-old rats were fed either a high-fat diet ( n = 30) or a normal diet ( n = 10) for 9, 13 or 17 weeks. The mRNA and protein levels for LXRα and SREBP-1c were measured at each time point, as was fatty acid synthase (FAS) activity and the serum levels of free fatty acid (FFA) and triglyceride (TG). The mRNA and protein levels for LXRα and SREBP-1c, FAS activity and serum levels of FFA and TG all significantly increased from week 9 in the high-fat diet rats versus controls. In conclusion, a high-fat diet upregulates LXRα which, in turn, upregulates SREBP-1c, increasing the activity of FAS and FFA and accumulation of TG in hepatocytes. Thus, LXRα and SREBP-1c contribute to the development of NASH.