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Supplemental Material for Epidemiology of Cervical Fracture/cervical Spinal Cord Injury and Changes in Surgical Treatment Modalities in elderly Individuals During a 10-year Period: A Nationwide Multicenter Study in Japan by Philipp Pieroh, Ulrich J.A. Spiegl, Anna Völker, Sven Märdian, Nicolas H. von der Höh, Georg Osterhoff, Christoph-E. Heyde; Spine Section of the German Society for Orthopaedics and Trauma in Global Spine Journal

Background Hypopituitarism is not well known after the treatment of a cavernous carotid aneurysm extending to the sellar region by the parent artery occlusion and bypass surgery. Case Description A 60-year-old female presented with a 2-year-old progressive visual disturbance. The patient had no pituitary hormone−related symptoms or signs, but elevated prolactin and decreased free thyroxin levels are shown on blood examination. Neuroimages revealed a right giant partially thrombosed cavernous carotid aneurysm compressing the sella turcica markedly. The aneurysm was treated by the right cervical internal carotid artery ligation with the right superficial temporal artery−middle cerebral artery double anastomoses. The patient had headache, general fatigue, chilling, and hypoactivity on postoperative day 6, when aneurysmal mass effects were transiently increased on neuroimages, associated with hypocortisolism and hyponatremia. Hydrocortisone administration improved the symptoms and was tapered off at 8 months post surgery, as the aneurysm shrank and pituitary hormone values were normalized except for prolactin. Conclusion Hypopituitarism should be taken into consideration even after the parent artery occlusion with bypass surgery for a giant carotid aneurysm compressing the sella turcica.

Supplemental Material, sj-pdf-1-mpx-10.1177_17448069211058004 for Endothelin receptor type A is involved in the development of oxaliplatin-induced mechanical allodynia and cold allodynia acting through spinal and peripheral mechanisms in rats by Kae Matsuura, Atsushi Sakai, Yuji Watanabe, Yasunori Mikahara, Atsuhiro Sakamoto and Hidenori Suzuki in Molecular Pain

Additional file 1: Figure S1. Activation of GIRK currents by 5-HT and baclofen in the prefrontal cortex L5 pyramidal neurons of WT male mice. A and B, 5-HT- and baclofen-induced currents were recorded by applying voltage ramps (− 140 to 0 mV, 500 ms) in the presence of 10 mM extracellular K+ in order to elicit K+ currents. Voltage-gated Na+ and Ca2+ currents and outward Ca2+-activated K+ currents were eliminated by simultaneous bath perfusion of TTX (0.5 μM) and cadmium (100 μM). Left panels: Current-voltage (I-V) relationships determined before (black traces) and after the application of agonists (red traces) by a constant voltage ramp command (upper trace). Right panels: agonist-sensitive currents were obtained by subtracting the current during the application of 5-HT (A) and baclofen (B) from the baseline shown in the Left panels. Under these conditions, 5-HT and baclofen induced currents that displayed .pronounced inward rectification and reversed polarity near the calculated equilibrium potential of − 68.9 mV (5-HT: − 61.6 ± 1.3 mV, n = 5; baclofen: − 64.0 ± 2.1 mV, n = 5) for a current carried by K+ ions. C and D, the effects of non-selective GIRK channel blocker, barium, on both 5-HT- and baclofen-induced outward currents. All recorded pyramidal neurons were Sag(+)-type neurons. The membrane currents were recorded at a holding potential of − 60 mV in the presence of 0.5 μM tetrodotoxin, in order to block spontaneous synaptic events. (C) Representative recordings of 5-HT-induced outward current pre- and post-barium treatment (Ba2+, 200 μM) and washout (> 15 min) in L5 pyramidal neurons. Right panels: Percent changes in the 5-HT-induced outward current amplitude. Ba2+ largely blocked the amplitude of the outward currents, which was partially recovered following washout (Ba2+: 26.6 ± 3.6%; Washout: 76.2 ± 7.0%, n = 5). (D) Representative recordings of baclofen-induced outward current pre- and post-Ba2+ treatment and washout (> 15 min) in L5 pyramidal neurons. Right panel: Percent changes in the baclofen-induced outward current amplitude. The baclofen-induced outward currents were also largely blocked by Ba2+ (Ba2+: 22.9 ± 1.7%; Washout: 78.5 ± 9.2%, n = 8).

Supplemental material, MPX904462 Supplemental material3 for Dorsal Root Ganglia Homeobox downregulation in primary sensory neurons contributes to neuropathic pain in rats by Takaya Ito, Atsushi Sakai, Motoyo Maruyama, Yoshitaka Miyagawa, Takashi Okada, Haruhisa Fukayama and Hidenori Suzuki in Molecular Pain

Supplemental material, MPX904462 Supplemental material1 for Dorsal Root Ganglia Homeobox downregulation in primary sensory neurons contributes to neuropathic pain in rats by Takaya Ito, Atsushi Sakai, Motoyo Maruyama, Yoshitaka Miyagawa, Takashi Okada, Haruhisa Fukayama and Hidenori Suzuki in Molecular Pain

Supplemental material, DS_10.1369_0022155419878181 for Morphological Characteristics of Neuronal Death After Experimental Subarachnoid Hemorrhage in Mice Using Double Immunoenzymatic Technique by Fumi Nakano, Lei Liu, Fumihiro Kawakita, Hideki Kanamaru, Yoshinari Nakatsuka, Hirofumi Nishikawa, Takeshi Okada, Masato Shiba and Hidenori Suzuki in Journal of Histochemistry & Cytochemistry

Materials and Methods. Supplementary Discussion. (DOCX 24 kb)

Functional classification of the genes that showed reduced responsiveness 1 h after repeated ECS treatments compared with a single ECS on the basis of the gene ontology (GO) terms. Table S2. Functional classification of the genes that showed similar responsiveness 1 h after repeated ECS treatments compared with a single ECS on the basis of the GO terms. Table S3. Functional classification of the genes that showed significantly increased expression change by only ECS treatment (317 gene probes) on the basis of the gene ontology (GO) terms. Table S4. Functional classification of the genes that showed significantly decreased expression change by only ECS treatment (444 gene probes) on the basis of the GO terms. Table S5. Functional classification of the genes that showed significantly increased expression change by only SSRI treatment (690 gene probes) on the basis of the GO terms. Table S6. Functional classification of the genes that showed significantly decreased expression change by only SSRI treatment (739 gene probes) on the basis of the GO terms. (PDF 169 kb)

Functional classification of the genes that showed reduced responsiveness 1 h after repeated ECS treatments compared with a single ECS on the basis of the gene ontology (GO) terms. Table S2. Functional classification of the genes that showed similar responsiveness 1 h after repeated ECS treatments compared with a single ECS on the basis of the GO terms. Table S3. Functional classification of the genes that showed significantly increased expression change by only ECS treatment (317 gene probes) on the basis of the gene ontology (GO) terms. Table S4. Functional classification of the genes that showed significantly decreased expression change by only ECS treatment (444 gene probes) on the basis of the GO terms. Table S5. Functional classification of the genes that showed significantly increased expression change by only SSRI treatment (690 gene probes) on the basis of the GO terms. Table S6. Functional classification of the genes that showed significantly decreased expression change by only SSRI treatment (739 gene probes) on the basis of the GO terms. (PDF 169 kb)