202 results on '"Taisheng Li"'
Search Results
2. CD4+ T cell counts and soluble programmed death-1 at baseline correlated with hepatitis B surface antigen decline in HIV/HBV coinfection during combined antiretroviral therapy
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Xiaodi Li, Ling Xu, Lianfeng Lu, Xiaosheng Liu, Yang Yang, Yuanni Wu, Yang Han, Xiaoxia Li, Yanling Li, Xiaojing Song, Wei Cao, and Taisheng Li
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Microbiology (medical) ,Infectious Diseases ,Immunology ,Microbiology - Abstract
BackgroundSeveral studies have described the rapid decline and clearance of hepatitis B surface antigen (HBsAg) in human immunodeficiency virus (HIV)/hepatitis B virus (HBV) coinfection after initiating combined antiretroviral therapy (cART). Early decline of HBsAg levels is associated with HBsAg seroclearance in the treatment of chronic HBV infection. This study aims to evaluate the HBsAg kinetics and the determinants of early HBsAg decline in patients with HIV/HBV coinfection during cART.MethodsA total of 51 patients with HIV/HBV coinfection were enrolled from a previously established HIV/AIDS cohort and followed for a median of 59.5 months after cART initiation. Biochemical tests, virology and immunology assessments were measured longitudinally. The kinetics of HBsAg during cART were analyzed. Soluble programmed death-1 (sPD-1) levels and immune activation markers (CD38 and HLA-DR) were measured at baseline, 1-year and 3-year during treatment. HBsAg response was defined as a decline of more than 0.5 log10 IU/ml at 6 months from the baseline after initiation of cART.ResultsHBsAg declined faster (0.47 log10 IU/mL) in the first six months and attained a decrease of 1.39 log10 IU/mL after 5-year therapy. Seventeen (33.3%) participants achieved a decline of more than 0.5 log10 IU/ml at the first 6 months of cART(HBsAg response) of which five patients achieved HBsAg clearance at a median of 11 months (range: 6-51 months). Multivariate logistic analysis showed the lower baseline CD4+ T cell levels (OR=6.633, P=0.012) and sPD-1 level (OR=5.389, P=0.038) were independently associated with HBsAg response after cART initiation. The alanine aminotransferase abnormality rate and HLA-DR expression were significantly higher in patients who achieved HBsAg response than in those who did not achieve HBsAg response after cART initiation.ConclusionLower CD4 + T cells, sPD-1, and immune activation were related to a rapid HBsAg decline in patients with HIV/HBV-coinfection after the initiation of cART. These findings imply that immune disorders induced by HIV infection may disrupt immune tolerance to HBV, leading to a faster decline in HBsAg levels during coinfection.
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- 2023
3. HIV DNA positively correlates with HLA-DR+CD8+ T lymphocytes over 8-year suppressive ART
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Tianyuan Zhu, Wei Cao, and Taisheng Li
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Infectious Diseases ,Immunology ,Immunology and Allergy - Published
- 2023
4. Effect of different antiretroviral therapy on muscle mass, bone mineral density, and trabecular bone score in Chinese HIV-infected males
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Zaizhu Zhang, Qiang Lin, Ying Xu, Wenmin Guan, Xiaojing Song, Yanling Li, Yi Zhang, Taisheng Li, and Wei Yu
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Orthopedics and Sports Medicine - Published
- 2023
5. Diagnosis and Clinical Management of Acute Severe Hepatitis of Unknown Origin: Operational Recommendation of Peking Union Medical College Hospital
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Wei Cao, Zhenghong Li, Huadong Zhu, Xiang Zhou, Qiwen Yang, Yang Han, Jihai Liu, Qing Chang, and Taisheng Li
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- 2022
6. <scp>HBV pgRNA</scp> profiles in Chinese <scp>HIV</scp> / <scp>HBV</scp> coinfected patients under pre‐ and posttreatment: a multicentre observational cohort study
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Ling, Xu, Xiaodi, Li, Lianfeng, Lu, Xiaosheng, Liu, Xiaojing, Song, Yanling, Li, Yang, Han, Ting, Zhu, Wei, Cao, and Taisheng, Li
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Cohort Studies ,Hepatitis B virus ,Hepatitis B Surface Antigens ,Hepatitis B, Chronic ,Infectious Diseases ,Hepatology ,Coinfection ,Virology ,DNA, Viral ,Humans ,RNA ,HIV Infections ,Hepatitis B e Antigens - Abstract
Data on hepatitis B virus (HBV) pregenomic (pgRNA) levels in HIV/HBV coinfected patients pre- and post-combined antiretroviral therapy (cART) are limited. This study aimed to evaluate the distribution of HBV pgRNA levels in treatment-naive coinfected patients and explore the changes that occur after the initiation of cART by examining patients from multicentre cohort studies performed in China. We included HIV/HBV coinfected subjects from the China AIDS Clinical Trial cohorts established from 2008 to 2014. Clinical and serological markers of HIV and HBV infection and biochemical data were acquired at baseline and after 96 and 240-480 weeks of cART. The correlations between HBV pgRNA and HBV DNA levels as well as HBsAg levels were calculated using Spearman's bivariate correlation analysis, and multivariate regression analysis was performed to determine factors associated with undetectable HBV pgRNA levels before cART and HBeAg loss after cART. A total of 132 HIV/HBV coinfected patients were enrolled, and 100 individuals were HBeAg-negative. A total of 34.4% (32/93) of patients were positive for HBV pgRNA, and the median HBV pgRNA level was 4.92 (IQR: 4.21-6.12) log
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- 2022
7. Metatranscriptomic analysis of host response and vaginal microbiome of patients with severe COVID-19
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Meng Xiao, Bo Lu, Rui Ding, Xia Liu, Xian Wu, Yaqian Li, Xudong Liu, Lin Qiu, Zhibo Zhang, Jing Xie, Yu Chen, Dong Zhang, Liting Dong, Meiling Zhang, Jinying Peng, Hua Yang, Timothy Kudihna, Yingchun Xu, Taisheng Li, Chengqi Yi, and Lan Zhu
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Microbiota ,COVID-19 ,Humans ,Female ,Metagenomics ,General Agricultural and Biological Sciences ,General Biochemistry, Genetics and Molecular Biology ,General Environmental Science - Published
- 2022
8. Comparison of Renal Function Biomarkers of Serum Creatinine and Cystatin C in HIV-Infected People on Dolutegravir-Containing Therapy
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Lianfeng Lu, Xiaodi Li, Xiaosheng Liu, Yang Han, Zhifeng Qiu, Xiaojing Song, Yanling Li, Xiaoxia Li, Wei Cao, and Taisheng Li
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Pharmacology ,Infectious Diseases ,Infection and Drug Resistance ,Pharmacology (medical) - Abstract
Lianfeng Lu,1 Xiaodi Li,1 Xiaosheng Liu,2 Yang Han,1 Zhifeng Qiu,1 Xiaojing Song,1 Yanling Li,1 Xiaoxia Li,1 Wei Cao,1 Taisheng Li1,2 1Department of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, Peopleâs Republic of China; 2Tsinghua-Peking Center for Life Sciences, Beijing, China Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, Peopleâs Republic of ChinaCorrespondence: Taisheng Li, Department of Infectious Diseases, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, 1# Shuai Fu Yuan, Beijing, 100730, Peopleâs Republic of China, Email litsh@263.netObjective(s): HIV-1-infected Chinese patients who were treated naïve with combination dolutegravir (DTG) and tenofovir disoproxil fumarate (TDF) group, DTG without TDF group, TDF without DTG, as well as patients switched to DTG-containing therapy from other drugs were included.Design: The dynamics of serum creatinine, cystatin C (CysC) level, eGFRcr and eGFRCysC at the baseline, 4 w, 12w, 24w, 36w and 48w for different group of patients were collected and evaluated.Methods: Changes in serum creatinine, levels, eGFRcr and eGFRCysC were analyzed among groups and in different time-points. Intra-group correlation coefficient and BlandâAltman plot were used to compare the results of eGFRcr and eGFRCysC.Results: Thirty-seven treated-naïve HIV-patients in combined DTG and TDF group (group 1), 23 in DTG without TDF patients (group 2) and 47 patients on TDF without DTG group (control group, group 3) along with 31 patients whose ART switch to DTG-containing regimens (group 4) were collected. Serum creatinine was significantly elevated in the group 1 and group 2 instead of group 3 from baseline to 48w. Mean decreased change of eGFR calculated by serum creatinine proved the same conclusion. However, there were no differences in serum cystatin C and eGFRCysC between baseline and at 48 weeks in DTG-containing groups. Moreover, the proportion of eGFRcr decreased over 30% was significantly higher in DTG-treatment group.Conclusion: We demonstrated the clinical benefits of CysC for assessing the glomerular filtration rate when evaluating renal function in HIV-1-infected patients treated with whether DTG combined with TDF or not.Keywords: HIV, antiretroviral therapy, dolutegravir, renal function, cystatin C, serum creatinine
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- 2022
9. Peripheral CD8+CD28+ T lymphocytes predict the efficacy and safety of PD-1/PD-L1 inhibitors in cancer patients
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Ruixuan Geng, Hui Tang, Tingting You, Xiuxiu Xu, Sijian Li, Zepeng Li, Yuan Liu, Wei Qiu, Na Zhou, Ningning Li, Yuping Ge, Fuping Guo, Yuhong Sun, Yingyi Wang, Taisheng Li, and Chunmei Bai
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Immunology ,Immunology and Allergy - Abstract
BackgroundProgrammed cell death protein-1/programmed cell death ligand-1 (PD-1/PD-L1) inhibitors works by reactivating immune cells. Considering the accessibility of noninvasive liquid biopsies, it is advisable to employ peripheral blood lymphocyte subsets to predict immunotherapy outcomes.MethodsWe retrospectively enrolled 87 patients with available baseline circulating lymphocyte subset data who received first-line PD-1/PD-L1 inhibitors at Peking Union Medical College Hospital between May 2018 and April 2022. Immune cell counts were determined by flow cytometry.ResultsPatients who responded to PD-1/PD-L1 inhibitors had significantly higher circulating CD8+CD28+ T-cell counts (median [range] count: 236 [30-536] versus 138 [36-460]/μL, p < 0.001). Using 190/μL as the cutoff value, the sensitivity and specificity of CD8+CD28+ T cells for predicting immunotherapy response were 0.689 and 0.714, respectively. Furthermore, the median progression-free survival (PFS, not reached versus 8.7 months, p < 0.001) and overall survival (OS, not reached versus 16.2 months, p < 0.001) were significantly longer in the patients with higher CD8+CD28+ T-cell counts. However, the CD8+CD28+ T-cell level was also associated with the incidence of grade 3-4 immune-related adverse events (irAEs). The sensitivity and specificity of CD8+CD28+ T cells for predicting irAEs of grade 3-4 were 0.846 and 0.667, respectively, at the threshold of CD8+CD28+ T cells ≥ 309/μL.ConclusionsHigh circulating CD8+CD28+ T-cell levels is a potential biomarker for immunotherapy response and better prognosis, while excessive CD8+CD28+ T cells (≥ 309/μL) may also indicate the emergence of severe irAEs.
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- 2023
10. Benefits of high-dose intravenous immunoglobulin on mortality in patients with severe COVID-19: An updated systematic review and meta-analysis
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Xiaosheng Liu, Yuelun Zhang, Lianfeng Lu, Xiaodi Li, Yuanni Wu, Yang Yang, Taisheng Li, and Wei Cao
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Immunology ,Immunology and Allergy - Abstract
BackgroundThe clinical benefits of high-dose intravenous immunoglobulin (IVIg) in treating COVID-19 remained controversial.MethodsWe systematically searched databases up to February 17, 2022, for studies examining the efficacy of IVIg compared to routine care. Meta-analyses were conducted using the random-effects model. Subgroup analysis, meta-regression, and trial series analysis w ere performed to explore heterogeneity and statistical significance.ResultsA total of 4,711 hospitalized COVID-19 patients (1,925 IVIg treated and 2786 control) were collected from 17 studies, including five randomized controlled trials (RCTs) and 12 cohort studies. The application of IVIg was not associated with all-cause mortality (RR= 0.89 [0.63, 1.26], P= 0.53; I2 = 75%), the length of hospital stays (MD= 0.29 [-3.40, 6.44] days, P= 0.88; I2 = 96%), the needs for mechanical ventilation (RR= 0.93 ([0.73, 1.19], P= 0.31; I2 = 56%), or the incidence of adverse events (RR= 1.15 [0.99, 1.33], P= 0.06; I2 = 20%). Subgroup analyses showed that overall mortality among patients with severe COVID-19 was reduced in the high-dose IVIg subgroup (RR= 0.33 [0.13, 0.86], P= 0.02, I2 = 68%; very low certainty).ConclusionsResults of this study suggest that severe hospitalized COVID-19 patients treated with high-dose IVIg would have a lower risk of death than patients with routine care.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021231040, identifier CRD42021231040.
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- 2023
11. Memory CD4+T cell profile is associated with unfavorable prognosis in IgG4-related disease: Risk stratification by machine-learning
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Yuxue Nie, Zheng Liu, Wei Cao, Yu Peng, Hui Lu, Ruijie Sun, Jingna Li, Linyi Peng, Jiaxin Zhou, Yunyun Fei, Mengtao Li, Xiaofeng Zeng, Wen Zhang, and Taisheng Li
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Immunology ,Immunology and Allergy - Published
- 2023
12. Comparison of Differences in Bone Mineral Density Measurement With 3 Hologic Dual-Energy X-Ray Absorptiometry Scan Modes
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Wei Yu, Wei Pan, Weibo Xia, Evelyn Hsieh, Zaizhu Zhang, Taisheng Li, Qiang Lin, and Wenmin Guan
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0301 basic medicine ,Endocrinology, Diabetes and Metabolism ,030209 endocrinology & metabolism ,Article ,Imaging phantom ,World health ,03 medical and health sciences ,Absorptiometry, Photon ,0302 clinical medicine ,Bone Density ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Orthopedics and Sports Medicine ,Densitometer ,Dual-energy X-ray absorptiometry ,Bone mineral ,Reproducibility ,Lumbar Vertebrae ,medicine.diagnostic_test ,business.industry ,Reproducibility of Results ,Single factor analysis ,Dual-Energy X-Ray Absorptiometry Scan ,Osteoporosis ,030101 anatomy & morphology ,business ,Biomedical engineering - Abstract
Bone mineral density (BMD) measurement using dual-energy X-ray absorptiometry (DXA) is considered a diagnostic parameter for osteoporosis by the World Health Organization (WHO). DXA densitometers have different scanning modes for BMD measurements, although the specific scanning modes vary based upon the manufacturer. For DXA machines manufactured by Hologic, which are used globally, a range of scanning modes exist, including but not limited to (in order of decreasing spatial resolution) Array, Fast Array, and Express Array. Only a handful of prior studies have compared the reproducibility of BMD measurements across scan modes. The present study aimed to add to this body of literature by investigating the differences in BMD measured between 3 scanning modes in Hologic DXA machines at 19 different health centers. As part of cross-calibration activities for two multi-center studies in China measuring BMD, the European spine phantom (ESP, 1.000 g/cm(2)) was scanned on 19 different Hologic DXA machines. To measure differences in BMD between the 3 scan modes most commonly found on the Hologic models available (i.e., Array, Fast Array, Express Array), the ESP measurement was performed 10 times for each scan mode on each Hologic DXA machine. One-sample t test was used to compare the average difference between the measured ESP results of the 3 scanning modes at each hospital and reference ESP values. Single factor analysis of variance was performed to compare the average differences between the pairs of scanning modes using the reference ESP. Statistically significant differences between the measured ESP results with reference ESP values were found with each scanning mode at 19 hospitals (all p values
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- 2021
13. Short-term and long-term outcomes in patients with cryptococcal meningitis after ventriculoperitoneal shunt placement
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Junxian, Wen, Rui, Yin, Jianbo, Chang, Yihao, Chen, Xiying, Dong, Wei, Cao, Xiaojun, Ma, Taisheng, Li, and Junji, Wei
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Neurology ,Neurology (clinical) - Abstract
ObjectiveThe purpose of this study was to assess the short-term and long-term outcomes of ventriculoperitoneal shunt (VPS) placement in patients with cryptococcal meningitis (CM).MethodsWe performed a retrospective analysis of all patients with CM admitted to the Peking Union Medical College Hospital from September 1990 to January 2021. We collected related clinical features to analyze the short- and long-term outcomes of VPS at 1 month and 1 year at least the following therapy, respectively. Overall survival (OS) was compared with all patients and a subgroup of critically ill cases by the Kaplan–Meier method with the log-rank test. Univariable and multivariable analyses were also performed using the Cox proportional hazard model to identify statistically significant prognostic factors.ResultsWe enrolled 98 patients, fifteen of whom underwent VPS. Those who received VPS had a lower cerebrospinal fluid (CSF) Cryptococcus burden (1:1 vs. 1:16; p = 0.046), lower opening pressures (173.3 mmH2 O vs. 224 mmH2O; p = 0.009) at lumbar punctures, and a lower incidence of critical cases (6.7 vs. 31.3%; p = 0.049). According to our long-term follow-up, no significant difference was shown in the Barthel Index (BI) between the two groups. Two patients in the VPS group suffered postoperative complications and had to go through another revision surgery. According to survival analysis, overall survival (OS) between the VPS and non-VPS groups was not significantly different. However, the Kaplan–Meier plots showed that critical patients with VPS had better survival in OS (p < 0.009). Multivariable analyses for critical patients showed VPS was an independent prognostic factor.ConclusionA VPS could reduce the intracranial pressure (ICP), decrease the counts of Cryptococcus neoformans by a faster rate and reduce the number of critical cases. The VPS used in critical patients with CM has a significant impact on survival, but it showed no improvement in the long-term Barthel Index (BI) vs. the conservative treatment and could lead to postoperative complications.
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- 2022
14. Sex differences in the association between cerebrovascular function and cognitive health in people living with HIV in urban China
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Felicia C. Chow, Fang Zhao, Yun He, Xiaojing Song, Jiangxia Zhang, Donghui Ao, Yuchen Wu, Bo Hou, Farzaneh A. Sorond, Beau M. Ances, Scott Letendre, Robert K. Heaton, Chuan Shi, Feng Feng, Yicheng Zhu, Huanling Wang, and Taisheng Li
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Infectious Diseases ,Pharmacology (medical) - Abstract
Cardiometabolic and cerebrovascular disease are strong independent contributors to cognitive irmpairment in people living with HIV (PWH). Data suggest that cardiovascular risk may play a greater role in cognitive health in women than men with HIV.We performed a cross-sectional study of 104 participants with virologically suppressed HIV from two clinics in urban China. Participants underwent neuropsychological testing from which we calculated T-scores globally and in 5 cognitive domains. We assessed cerebral vasoreactivity of the middle cerebral arteries in response to breath-holding. We constructed linear regression models to determine associations between cerebrovascular and cognitive function overall and stratified by sex.Women were younger than men (48 versus 51 years, p=0.053), had fewer years of education (9 years versus 12 years, p=0.004), and fewer cardiometabolic risk factors (0 versus 1 factor, p=0.008). In a model with all participants, cerebrovascular function was significantly associated with global cognition (2.74 higher T-score per 1-point higher cerebral vasoreactivity [SE 1.30], p=0.037). Cerebrovascular function remained significantly associated with global cognition among women (4.15 higher T-score [SE 1.78], p=0.028) but not men (1.70 higher T-score [SE 1.74], p=0.33). The relationships between cerebrovascular function and specific cognitive domains followed a similar pattern, with significant associations present among women but not men.Women with well-controlled HIV may be more vulnerable to the impact of cerebrovascular injury on cognitive health than men. Studies evaluating strategies to protect against cognitive impairment in PWH should include adequate representation of women and stratification of analyses by sex.
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- 2022
15. Bond Stress-Slip Model of BFRP Grid to ECC
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Langni Deng, Taisheng Li, Mengjun Zhong, Ling Liao, and Hua Li
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General Materials Science ,BFRP grid ,ECC ,composite materials ,bond–slip ,constitutive model ,anchorage length - Abstract
The bonding performance between a basalt fiber-reinforced composite material (BFRP) grid and an engineering cementitious composite (ECC) is the basis that affects the synergy between the two. However, the research on the bonding behavior between the FRP grid and ECC is limited; in particular, the theoretical study on the bond–slip intrinsic relationship model and a reliable anchorage length calculation equation is lacking. To study the bond–slip relationship between the BFRP grid and ECC material, we considered the parameters of BFRP grid thickness, anchorage length, ECC substrate protective layer thickness, and grid surface treatment, and conducted center pull-out tests on eight sets of specimens. By analyzing the characteristics of the bond–slip curve of the specimen, a bond–slip constitutive model between the BFRP grid and ECC was established. Combining the principle of equivalent strain energy, the calculation formula of the basic anchorage length of the BFRP grid in the ECC matrix was derived. Research shows that the bonding performance between the BFRP grid and ECC improves with the increase in the grid anchoring length, grid thickness, and ECC layer strength. Sand sticking on the surface of the BFRP grid can enhance the bonding force between the two. The established bond–slip constitutive model curve is in good agreement with the test curve. The bond–slip relationship between the BFRP grid and ECC can be described by the first two stages in the BPE model. The derived formula for calculating the basic anchorage length of the BFRP mesh in the ECC matrix is computationally verified to be reliable in prediction.
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- 2022
16. Risk factors and longitudinal changes of dyslipidemia among adult Chinese HIV-1 Patients Receiving Antiretroviral Therapy
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Xiuxia Li, Xiaojing Song, Yang Han, Zhifeng Qiu, Wei Cao, and Taisheng Li
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Background Despite an apparent decline in mortality of HIV-infected individuals due to the broad utilization of antiretroviral therapy (ART), life-long treatment is required accompanying with various metabolic abnormalities. Data about the epidemiology and the dynamic change of dyslipidemia in HIV-1 patients receiving antiretroviral therapy was restricted in Asian countries. The purpose was to explore the specific situation of dyslipidemia in adult Chinese HIV-1 Patients upon a multicenter clinical trial. Methods We conducted a retrospective cross-sectional analysis on patients enrolled in two large multicenter clinical trials across China and patients followed in the clinic of Peking Union Medical College Hospital (PUMCH). Demographic data and clinical parameters were collected, risk factors and longitudinal changes of lipid profiles under different clinical settings associated with HIV-1 infection were analyzed. The definition of dyslipidemia was made based upon the National Cholesterol Education Program, Adult Treatment Panel (NCEP-ATP) III guidelines. Results A total of 1942 patients were enrolled. The median follow-up was 6 years. At baseline, the concentrations of total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were 4.1 ± 0.96 mmol/L, 1.24 (interquartile ranges [IQR] 0.87–1.8) mmol/L, 1.11 ± 0.33 and 2.38 ± 0.78 mmol/L, respectively. The rate of hypercholesterolemia, hyperglyceridemia, high LDL-C and low HDL-C were 11.38%, 28.16%, 45.66% and 9.29%, respectively. The overall prevalence of dyslipidemia was 69.3%, which raised to 84.3% after antiretroviral therapy, substantially higher. Risk factors of CD4/CD8 ratio less than 0.3 and viral load over 105 copies/mL for all types of dyslipidemia as well as a negative correlation between HDL-C concentration and CD8 + CD38 + percentage were found. Besides, regimens including efavirenz (EFV) and tenofovir (TDF) performed better lipid profiles. Longitudinal analysis revealed that both the rate of abnormal lipid indexes and concentration of lipid changed steeply in the first 6 months after ART initiation. Conclusions The prevalence of dyslipidemia was high in HIV-1 patients and elevated after antiretroviral therapy, mainly represented as high TG and low HDL-C, associating with advanced stage of HIV-1 infection. Lipid changed remarkably in the initial stage after ART therapy.
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- 2022
17. Diagnosis and Treatment Guidelines for Mesenchymal Stem Cell Therapy for Coronavirus Disease 2019 (Beijing, 2021)
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Weidong Han, Gui-Qiang Wang, Shufang Meng, Lei Shi, Junliang Fu, Charlie Xiang, Chen Yao, Yu Zhang, Wei-Fen Xie, Zhaohui Wu, Yonggang Li, Zunyou Wu, Tao Cheng, Ruonan Xu, Taisheng Li, Xin Yuan, Yuanyuan Li, Wen-Hong Zhang, Qi Zhou, Chunhua Zhao, Zhongmin Liu, Chenyan Gao, Yuquan Wei, Yu Hu, Fanping Meng, Zhe Xu, Yunxia Sun, Fu-Sheng Wang, Ming Shi, Lei Huang, and Xiaoying Wang
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Coronavirus disease 2019 (COVID-19) ,Beijing ,business.industry ,Mesenchymal stem cell ,Medicine ,business ,Virology - Published
- 2021
18. Combination of Tripterygium Wilfordii Hook F With Antiretroviral Therapy Delayed Viral Rebound in A Patient of Acute HIV-1 Infection
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Zhifeng Qiu, Yang Han, Huiling Weng, Jing Xie, Zhibiao Mai, Jean-Pierre Routy, Tong Wang, Xiaojing Song, Wei Lyu, Jianhua Wang, Yizhi Cui, Yongsong Yue, Taisheng Li, Gong Zhang, and Wei Cao
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Viral rebound ,biology ,Hook ,business.industry ,Human immunodeficiency virus (HIV) ,medicine ,Tripterygium wilfordii ,medicine.disease_cause ,business ,biology.organism_classification ,Virology ,Antiretroviral therapy - Published
- 2021
19. Cytomegalovirus coinfection among people living with HIV in China before and on cART: A retrospective study
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Xiaodi Li, Lianfeng Lu, Xiaosheng Liu, Yang Han, Ting Zhu, Zhifeng Qiu, Xiaoxia Li, Yanling Li, Xiaojing Song, Wei Cao, and Taisheng Li
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Background Cytomegalovirus (CMV) was the common opportunistic pathogen among people living with HIV(PLWH), even in the combination antiretroviral therapy (ART) era. This study aimed to report the epidemiology and characteristics of CMV coinfection among Chinese PLWH. Methods Data were collected from two prospective longitudinal multicenter cohorts of PLWH initiating combination antiretroviral therapy (cART) from 2010 to 2015. This study extracted laboratory parameters, clinical symptoms, and serology of CMV baseline to 192 weeks of cART. Results 1552 patients were included in this analysis; the prevalence of CMV coinfection was 3.53% before cART. Logistics analysis showed that patients with lower CD4 T cell counts, RPR-seropositive, infected through homosexual contact, and from southern China had a higher risk of CMV/HIV coinfection at baseline. Patients with CMV coinfection at baseline had lower CD4/CD8 ratios at baseline (0.29 vs 0.22, p = 0.006), 96 weeks (0.63 vs 0.51, p = 0.015). After initiation of cART, the incidence of CMV recent infection was 10 cases/1000 PY. Cases coinfected with CMV during cART were mostly asymptomatic and had a comparable CD4, CD8 T cell counts, and CD4/CD8 ratio to non-coinfected patients. Conclusion This multicenter study reported a national prevalence and incidence of CMV recent infection among the Chinese PLWH before or on cART. CMV coinfection before cART initiation was associated with a slower recovery of CD4/CD8 ratio. However, CMV coinfection during successful treatment cART seems not to affect immunity parameters.
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- 2022
20. The pattern and magnitude of T cell subsets reconstitution during ten years of ART with viral suppression in HIV-infected patients
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Lianfeng Lu, Xiaodi Li, Xiaosheng Liu, Zhifeng Qiu, Yang Han, Xiaojing Song, Yanling Li, Xiaoxia Li, Wei Cao, Wei Lv, Zhihui Dou, and Taisheng Li
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Aging ,Cell Biology - Abstract
The extent of immune reconstitution in human immunodeficiency virus (HIV) infected persons receiving long-term antiretroviral therapy (ART) with controlled viral load has been controversial. We studied the extent and speed of T cell subsets retrieval after long-term antiretroviral treatment.662 HIV-infected patients followed at least 2 years whose plasma HIV-1 RNA load50 copies/mL were evaluated for longitudinal and functional phenotypic indices of immune restoration. Determinants of change in magnitude and importance of recovery have been evaluated using mixed linear regression models.Almost all robust immune restorations achieved occurred after 2-3 years of ART. The median CD4 lymphocyte count increased 449 cells/μl (IQR 303-604) from 226 cells/μl (IQR 83-336) at baseline during the third year (Immune restoration was dependent on the capacity of immune system during the first 2-3 year of ART. But the significant change of CD4 and compartments of CD4+T cells could persist until 6-8 years. The pattern of CD38+CD8+, HLA-DR+CD8+, CD28+CD4+ T cells could quickly return to normal level and no significant change after sufficient time of ART. In general, the immune response compared to the baseline status may be the overall effect from the age and time of antiretroviral treatment.
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- 2022
21. Therapeutic effect of (5R)-5-hydroxytriptolide (LLDT-8) in SIV infected rhesus monkeys
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Tingxia Lv, Wei Cao, Jing Xue, Qiang Wei, Zhifeng Qiu, Yang Han, and Taisheng Li
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Pharmacology ,Male ,Immunology ,Simian Acquired Immunodeficiency Syndrome ,Immunology and Allergy ,Animals ,Humans ,RNA ,HIV Infections ,Simian Immunodeficiency Virus ,Diterpenes ,Viral Load ,Macaca mulatta - Abstract
Human immunodeficiency virus (HIV) infections induce robust, generalized inflammatory responses and lead to pathological systemic immune activation. This abnormal immune status persists despite successful antiretroviral therapy (ART). Immune modulating strategies in conjunction with ART were tried to reduce abnormal immune activation. Previously, we demonstrated that Tripterygium Wilfordii Hook F has been shown immunosuppressive activity in HIV patients. (5R)-5-hydroxytriptolide (LLDT-8), a new analog of triptolide, and the most active ingredient of Tripterygium Wilfordii Hook F, has been shown to have lower cytotoxicity. However, the role of LLDT-8 in HIV or simian immunodeficiency virus (SIV) needs to be explored.Six male adult Chinese rhesus monkeys were enrolled in our study. All of them were healthy and negative for SIV, and chronically SIVmac239 infected macaques were treated with LLDT-8 combined with ART (n = 4) or ART only (n = 2) after 14 weeks of infection. ART was determined at week 33, and LLDT-8 was continued until week 48. T cell immune activation and inflammation were compared during the period, and viral rebound time and reservoir were supervised after stopping ART.The RNA level of the two groups continued to decline after initiating ART, RNA of 4 rhesus monkeys declined to the lower limit of detection at week 20. LLDT-8 administration combined with ART did not affect T cell activation and plasma levels of IL-6 and CRP. The viral load of all the macaques in both groups was rebounded 2 weeks after ART discontinuation. Furthermore, no significant decrease of SIV DNA was observed in the LLDT-8 treatment group.LLDT-8 administration during chronic SIV infection had no effect on T cell activation and plasma levels; Furthermore, LLDT-8 may not contribute to suppression of viral rebound and reservoir. These results suggest that LLDT-8 is unlikely to reduce immune activation and viral persistence without additional interventions.
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- 2022
22. Myocardial Injury on Admission as a Risk in Critically Ill COVID-19 Patients: A Retrospective in-ICU Study
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Wei Wu, Xufei Yang, Xiaowei Yan, Xiang Zhou, Shuyang Zhang, Zhengyin Liu, Yanjun Song, Fan Guo, Jinglan Wang, Long Chang, Yan Qin, Hao Qian, Taisheng Li, Ran Tian, and Peng Gao
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Adult ,Male ,medicine.medical_specialty ,Critical Illness ,critically ill ,Population ,030204 cardiovascular system & hematology ,law.invention ,03 medical and health sciences ,Patient Admission ,0302 clinical medicine ,Risk Factors ,030202 anesthesiology ,Interquartile range ,law ,Internal medicine ,medicine ,Risk of mortality ,Humans ,Risk factor ,education ,Aged ,Retrospective Studies ,Aged, 80 and over ,cardiovascular complication ,education.field_of_study ,business.industry ,Incidence (epidemiology) ,Hazard ratio ,COVID-19 ,novel coronavirus disease ,Middle Aged ,medicine.disease ,Intensive care unit ,Comorbidity ,Intensive Care Units ,Anesthesiology and Pain Medicine ,Cardiovascular Diseases ,Myocardial injury ,Female ,Original Article ,Cardiology and Cardiovascular Medicine ,business - Abstract
Highlights • Myocardial injury is common in critically ill COVID-19 patients. • COVID-19 patients with myocardial injury are more likely to develop adverse events and fatal outcomes during the hospitalization. • Myocardial injury and advanced age (≥75 yrs old) are independent risk factors for 28-day in-ICU mortality., Objective The aim of this study was to investigate the incidence, clinical presentation, cardiovascular (CV) complications and mortality risk of myocardial injury on admission in critically ill ICU inpatients with COVID-19. Design A single-center, retrospective, observational study. Setting A new-built ICU in Tongji hospital (Sino-French new city campus), Huazhong University of Science and Technology, Wuhan, China. Participants Seventy-seven critical COVID-19 patients. Interventions Patients were divided into myocardial injury group and non-myocardial injury group according to the on-admission levels of high-sensitivity cardiac troponin I. Measurements and Main Results Demographic data, clinical characteristics, laboratory tests, treatment and clinical outcome were evaluated stratified by the presence of myocardial injury on admission. Compared with non-myocardial injury patients, patients with myocardial injury were older (68.4 ± 10.1 vs. 62.1 ± 13.5 years; P=0.02), had higher prevalence of underlying CV disease (34.1% vs. 11.1%; P=0.02) and in-ICU CV complications (41.5% vs13.9%; P=0.008), higher Acute Physiology and Chronic Health Evaluation II scores (20.3 ± 7.3 vs 14.4 ± 7.4; P=0.001) and Sequential Organ Failure Assessment scores [7, interquartile range (IQR) 5-10 vs. 5,IQR 3-6; P
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- 2021
23. Absorbing filter AN69 surface treatment in critically ill COVID-19 patients: a single-center experience
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Yan Qin, Xiaowei Yan, Yan Hu, Xiang Zhou, Shuyang Zhang, Jie Ma, Limeng Chen, Gang Chen, Zhengyin Liu, Xuemei Li, Peng Xia, and Taisheng Li
- Subjects
Male ,China ,2019-20 coronavirus outbreak ,medicine.medical_specialty ,Letter ,Coronavirus disease 2019 (COVID-19) ,Critical Illness ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Critical Care and Intensive Care Medicine ,Single Center ,law.invention ,Renal Dialysis ,law ,Humans ,Medicine ,Letter to the Editor ,Aged ,SARS-CoV-2 ,urogenital system ,business.industry ,Critically ill ,Acute kidney injury ,COVID-19 ,Membranes, Artificial ,General Medicine ,Acute Kidney Injury ,Length of Stay ,medicine.disease ,Intensive care unit ,Diseases of the genitourinary system. Urology ,Intensive Care Units ,Nephrology ,Emergency medicine ,Female ,RC870-923 ,Cytokine Release Syndrome ,business ,Biomarkers - Abstract
Dear Editors,More than 20% of patients with coronavirus disease 2019 (COVID-19) in the intensive care unit (ICU) developed acute kidney injury (AKI) and worsened clinical outcomes [1]. Cytokine sto...
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- 2021
24. Gut lactate-producing bacteria promote CD4 T cell recovery on Anti-retroviral therapy in HIV-infected patients
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Qian Zhou, Routy Jean-Pierre, Zhifeng Qiu, Yang Han, Jing Li, Qingren Meng, Jian Wang, Jingfa Xiao, Chu Yanan, Shao Changjun, Jun Yu, Wei Lyu, Taisheng Li, Hua Zhu, Xiaojing Song, and Yu Kang
- Subjects
Streptococcus thermophilus ,Immune recovery ,Biophysics ,Gut flora ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Structural Biology ,Lactic acid bacteria ,Genetics ,Hiv infected patients ,ComputingMethodologies_COMPUTERGRAPHICS ,030304 developmental biology ,0303 health sciences ,biology ,HIV ,biology.organism_classification ,Computer Science Applications ,Viral replication ,030220 oncology & carcinogenesis ,Immunology ,Metagenome ,Antiretroviral medication ,TP248.13-248.65 ,Bacteria ,Research Article ,Biotechnology - Abstract
Graphical abstract, Anti-retroviral therapy (ART) effectively suppresses viral replication in HIV-infected patients, however CD4 + cell restoration to normal value is not achieved by 15–20% of patients who are called immune non-responders. Gut microbiota composition has been shown to influence host immunity. Herein, to identify intestinal microbial agents that may influence the CD4 recovery in HIV-infected patients, we utilized a “Quasi-paired cohort” method to analyze intestinal metagenome data from immunological responders (IRs) and immunological non-responders (INRs). This method identified significant enrichment for Streptococcus sp. and related lactate-producing bacteria (LAB) in IRs. In a validation cohort, positive correlations between the abundance of these LAB and the post-ART CD4 + recovery was observed, and a prediction model based on these LAB performed well in predicting immune recovery. Finally, experiments using a germ-free mouse model of antibody-induced CD4 + cell depletion showed that supplementation with a lactate-producing commensal Streptococcus thermophilus strongly promoted CD4 recovery. In conclusion, our study identified a group of LAB that was associated with enhanced immune recovery in post-ART HIV-infected patients and promotes CD4 + cell restoration in a mouse model. These findings favour supplementation of LAB commensal as a therapeutic strategy for CD4 + cell count improvement in HIV-infected patients.
- Published
- 2021
25. (5R)-5-hydroxytriptolide for HIV immunological non-responders receiving ART: a randomized, double-blinded, placebo-controlled phase II study
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Wei Cao, Xiaosheng Liu, Yang Han, Xiaojing Song, Lianfeng Lu, Xiaodi Li, Ling Lin, Lijun Sun, An Liu, Hongxin Zhao, Ning Han, Hongxia Wei, Jian Cheng, Biao Zhu, Min Wang, Ying Li, Ping Ma, Liying Gao, Xicheng Wang, Jianhua Yu, Ting Zhu, Jean-Pierre Routy, Min Zuo, and Taisheng Li
- Subjects
Psychiatry and Mental health ,Infectious Diseases ,Health Policy ,Pediatrics, Perinatology and Child Health ,Public Health, Environmental and Occupational Health ,Internal Medicine ,Obstetrics and Gynecology ,Geriatrics and Gerontology - Published
- 2023
26. Extinguish the Fire: Anti-inflammatory Strategies for Over Immune Activation in Chronic HIV-1 Infection
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Taisheng Li and Wei Cao
- Subjects
medicine.drug_class ,business.industry ,Immunology ,Human immunodeficiency virus (HIV) ,medicine ,medicine.disease_cause ,business ,Anti-inflammatory ,Immune activation - Published
- 2021
27. Antiphospholipid Antibodies in Critically Ill Patients With COVID‐19
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Meng Xiao, Yongzhe Li, Jie Ma, Dong Wu, Jing Xie, Xuzhen Qin, Huan Chen, Jinglan Wang, Xin Ding, Wei Wu, Yongqiang Zhao, Shulan Zhang, Yu Chen, Peng Gao, Xian Wu, Yan Qin, Zhengyin Liu, Hongmin Zhang, Shuyang Zhang, Ran Tian, Xuefeng Sun, Taisheng Li, Fengchun Zhang, Yan Zhang, Wen Zhang, Dong Zhang, Hua Zhao, Xin Hou, Wei Cao, Huadong Zhu, Wei Jiang, Peng Xia, Minya Lu, Xiaowei Yan, Jing Zhao, Bin Du, Chunyao Wang, Xiang Zhou, and Yingchun Xu
- Subjects
Adult ,Male ,Adolescent ,Critical Illness ,critically ill patients ,Immunology ,030204 cardiovascular system & hematology ,medicine.disease_cause ,coagulopathy ,Pathogenesis ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,COVID‐19 ,immune system diseases ,Antiphospholipid syndrome ,medicine ,Coagulopathy ,Humans ,Immunology and Allergy ,neoplasms ,Aged ,Coronavirus ,Aged, 80 and over ,030203 arthritis & rheumatology ,biology ,Cerebral infarction ,business.industry ,Brief Report ,Incidence (epidemiology) ,antiphospholipid antibodies ,COVID-19 ,Middle Aged ,medicine.disease ,Titer ,Antibodies, Antiphospholipid ,biology.protein ,Female ,Brief Reports ,Antibody ,business - Abstract
OBJECTIVE: Coagulopathy is one of the characteristics observed in critically ill patients with coronavirus disease 2019 (COVID-19). Antiphospholipid antibodies (aPLs) contribute to coagulopathy, though their role in COVID-19 remains unclear. This study was undertaken to determine the prevalence and characteristics of aPLs in patients with COVID-19. METHODS: Sera collected from 66 COVID-19 patients who were critically ill and 13 COVID-19 patients who were not critically ill were tested by chemiluminescence immunoassay for anticardiolipin antibodies (aCLs), anti-s2 -glycoprotein I (anti-s2 GPI) (IgG, IgM, and IgA), and IgG anti-s2 GPI-domain 1 (anti-s2 GPI-D1) and IgM and IgG anti-phosphatidylserine/prothrombin (anti-PS/PT) antibodies were detected in the serum by enzyme-linked immunosorbent assay. RESULTS: Of the 66 COVID-19 patients in critical condition, aPLs were detected in 31 (47% ). Antiphospholipid antibodies were not present among COVID-19 patients who were not in critical condition. The IgA anti-s2 GPI antibody was the most commonly observed aPL in patients with COVID-19 and was present in 28.8% (19 of 66) of the critically ill patients, followed by IgA aCLs (17 of 66, or 25.8%) and IgG anti-s2 GPI (12 of 66, or 18.2%). For multiple aPLs, IgA anti-s2 GPI + IgA aCLs was the most common antibody profile observed (15 of 66, or 22.7%), followed by IgA anti-s2 GPI + IgA aCL + IgG anti-s2 GPI (10 of 66, or 15.2%). Antiphospholipid antibodies emerge ~35-39 days after disease onset. A dynamic analysis of aPLs revealed 4 patterns based on the persistence or transient appearance of the aPLs. Patients with multiple aPLs had a significantly higher incidence of cerebral infarction compared to patients who were negative for aPLs (P = 0.023). CONCLUSION: Antiphospholipid antibodies were common in critically ill patients with COVID-19. Repeated testing demonstrating medium to high titers of aPLs and the number of aPL types a patient is positive for may help in identifying patients who are at risk of developing cerebral infarction. Antiphospholipid antibodies may be transient and disappear within a few weeks, but in genetically predisposed patients, COVID-19 may trigger the development of an autoimmune condition similar to the antiphospholipid syndrome (APS), referred to as "COVID-19-induced APS-like syndrome." Long-term follow-up of COVID-19 patients who are positive for aPLs would be of great importance in understanding the pathogenesis of this novel coronavirus.
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- 2020
28. The spectrum of kidney biopsy findings in Chinese HIV-infected patients
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Jie Ma, Qingyue Zheng, Hanlin Zhang, Yubing Wen, Wenling Ye, Wei Ye, Ke Zheng, Ruitong Gao, Limeng Chen, Taisheng Li, Xuemei Li, Yang Yu, and Wei Lyu
- Subjects
Male ,Infectious Diseases ,Health Policy ,Biopsy ,Humans ,Pharmacology (medical) ,Female ,Glomerulonephritis, IGA ,HIV Infections ,AIDS-Associated Nephropathy ,Kidney ,Retrospective Studies - Abstract
HIV-associated kidney disease is common but data on the pathology spectrum of kidney biopsy in China is lacking. This study aimed to illustrate the clinical presentation, laboratory findings and pathological spectrum of different subtypes of HIV-associated kidney disease in China.Eighteen HIV patients with renal biopsy indications at the Peking Union Medical College Hospital from January 2002 to October 2021 were retrospectively enrolled. All had CD4 counts and HIV viral load measurements. Renal biopsies were examined with light microscopy, immunofluorescence, and electron microscopy. Shapiro-Wilk test was used to test whether the data was normally distributed. The data is presented as medians (interquartile range), number (%), or means (±SD) according to their distribution.Seventeen patients had glomerular disease, and one patient had interstitial nephritis. Membranous nephropathy was present in eight patients (47.1%), and IgA nephropathy in four patients (23.5%). The difference in urine protein and serum albumin before and after treatment was statistically significant and no deaths or dialysis were observed to the end of follow-up.This study found that classic HIV-associated nephropathy (HIVAN) was uncommon in Chinese HIV patients. HIV immune complex kidney (HIVICK) disease, such as membranous or IgA nephropathy, was more common, and associated with better prognosis. Antiretroviral therapy, ACE inhibitors, and angiotensin II receptor blockers were effective in decreasing proteinuria and preserving renal function. The use of corticosteroids and immunosuppressive agents seems safe. However, the nephrotoxic effect of antiretroviral agents and other medications should be carefully monitored.
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- 2022
29. Longitudinal change in bone mineral density among Chinese individuals with HIV after initiation of antiretroviral therapy
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Yanling Li, Fuping Guo, Taisheng Li, Wei Pan, Wenmin Guan, Xiaojing Song, Wei Yu, and Evelyn Hsieh
- Subjects
Adult ,Male ,0301 basic medicine ,China ,medicine.medical_specialty ,Asia ,Anti-HIV Agents ,Endocrinology, Diabetes and Metabolism ,Osteoporosis ,Human immunodeficiency virus (HIV) ,HIV Infections ,030209 endocrinology & metabolism ,medicine.disease_cause ,Article ,03 medical and health sciences ,0302 clinical medicine ,Bone Density ,Internal medicine ,medicine ,Humans ,Retrospective Studies ,Femoral neck ,Bone mineral ,business.industry ,Middle Aged ,medicine.disease ,Rheumatology ,medicine.anatomical_structure ,Relative risk ,Orthopedic surgery ,030101 anatomy & morphology ,business ,Viral load - Abstract
This is the first study to report changes in BMD and related risk factors among Chinese patients with HIV after initiation of tenofovir disoproxil fumarate (TDF)-containing antiretroviral therapy. Greater bone mineral density (BMD) loss was observed in patients treated with TDF, compared to those on non-TDF-containing regimens. Our findings provide important knowledge regarding the risk factors in the long-term clinical management of patients with HIV in China. Persons living with HIV (PLWH) are at increased risk for osteoporosis and fracture. Tenofovir disoproxil fumarate (TDF) has been associated with higher rates of bone mineral density (BMD) loss, osteoporosis, and fracture. Few studies have studied the impact among PLWH in Asia. We analyzed retrospectively patients from the outpatient HIV clinic of a large tertiary hospital in Beijing, China, from March 2007 to May 2016. Patients who had dual-energy X-ray absorptiometry testing prior to antiretroviral initiation and at 48 and/or 96 weeks after initiation were included in this analysis. A total of 136 patients were included (mean age 36.0 ± 10.6 years) and over 90% participants were male and Han Chinese ethnicity. We observed greater declines in BMD at the spine from baseline to week 48 (−2.94% vs. −0.74%) and at the hip from baseline to week 96 (−4.37% vs. −2.34%) in the TDF group compared with the non-TDF group. With regard to HIV-specific parameters, longer duration since HIV diagnosis and undetectable viral load over time were associated with lower BMD at the hip [relative risk (RR) 0.97, 95% confidence index (CI) (0.95, 0.99) per 1 year increase and RR 0.96, 95%CI (0.94, 0.99), respectively] and femoral neck [RR 0.97, 95%CI (0.95, 0.99) per 1 year increase and RR 0.97, 95%CI (0.95, 0.998), respectively] over 96 weeks. This is the first study to report changes in BMD among PLWH after initiation of TDF-based antiretroviral therapy in China. Our findings provide important knowledge for the long-term clinical management of PLWH from this region.
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- 2020
30. Profile of natural anticoagulant, coagulant factor and anti-phospholipid antibody in critically ill COVID-19 patients
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Tienan Zhu, Zhengyin Liu, Wei Cao, Taisheng Li, Ning Tang, Xiaowei Yan, Meng Xiao, Yan Zhang, Wei Jiang, Yongzhe Li, and Yongqiang Zhao
- Subjects
Male ,030204 cardiovascular system & hematology ,Protein S ,law.invention ,chemistry.chemical_compound ,0302 clinical medicine ,Risk Factors ,Coagulopathy ,law ,Coagulation testing ,Antithrombin Proteins ,030212 general & internal medicine ,Blood Coagulation Factor Inhibitors ,Factor VII ,biology ,Antithrombin ,Anticoagulant ,Factor V ,Hematology ,Middle Aged ,Intensive care unit ,Blood Coagulation Factors ,Critical ,Host-Pathogen Interactions ,Antibodies, Antiphospholipid ,Female ,Coronavirus Infections ,Cardiology and Cardiovascular Medicine ,medicine.drug ,China ,medicine.medical_specialty ,medicine.drug_class ,Critical Illness ,Pneumonia, Viral ,Article ,Fibrin Fibrinogen Degradation Products ,Betacoronavirus ,03 medical and health sciences ,Internal medicine ,medicine ,Humans ,Blood Coagulation ,Pandemics ,Aged ,Antiphospholipid antibody ,Factor VIII ,SARS-CoV-2 ,business.industry ,COVID-19 ,Thrombosis ,medicine.disease ,Cross-Sectional Studies ,chemistry ,Hemostasis ,biology.protein ,business ,Biomarkers ,Protein C - Abstract
The outbreak of novel coronavirus disease 2019 (COVID-19) has now become a global pandemic. Coagulopathy has been reported widely in critically ill COVID-19 patients and was related to high mortality. However, the comprehensive coagulation profiles have not been examined and the underlying mechanism of the coagulopathy in COVID-19 patients is unclear. To study the coagulation profiles of routine hemostasis tests, natural anticoagulants, coagulant factors and antiphospholipid antibodies in critically ill COVID-19 patients. This single-center and cross-section study included 19 patients with COVID-19, who were admitted to intensive care unit (ICU) at Tongji hospital in Wuhan, China, from Feb 23 to Mar 3, 2020. Demographic data, laboratory parameters, treatments and clinical outcomes of the patients were collected and analyzed. The final date of follow-up was Mar 31, 2020. In this study, 12 thrombotic events occurred in 9 patients, including 4 cerebral infarctions, 7 acro-ischemia and 1 internal jugular vein thrombosis. The common abnormalities of routine coagulation tests included evelated D-Dimer level (100%), prolonged prothrombin time (73.7%) and hyperfibrinogenemia (73.7%). The median activities of natural anticoagulants including protein C, protein S and antithrombin were all below the normal range. Factor VIII activities were significantly above normal range (median value 307%, IQR 198–441) in all patients. Factor V and factor VII activities were significantly lower in near-terminal stage patients. Anti-phospholipid antibodies were present in 10 patients. Strikingly, 4 cerebral infarction events were in patients had anti-phospholipid antibodies of multiple isotypes. Sustained hypercoagulable status and thrombotic events were common in critically ill patients with COVID-19. The low activities of natural anticoagulants, elevated factor VIII level and the presence of antiphospholipid antibodies, together, may contribute to the etiopathology of coagulopathy in COVID-19 patients.
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- 2020
31. SARS-CoV-2 Is Not Detectable in the Vaginal Fluid of Women With Severe COVID-19 Infection
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Meng Xiao, Abraham Morse, Taisheng Li, Lan Zhu, Xia Liu, Yuhua Xie, Zhengyin Liu, Wei Cao, Jing Xie, and Lin Qiu
- Subjects
Microbiology (medical) ,Body fluid ,medicine.medical_specialty ,030219 obstetrics & reproductive medicine ,business.industry ,Retrospective cohort study ,medicine.disease ,Intensive care unit ,Virus ,law.invention ,03 medical and health sciences ,Pneumonia ,0302 clinical medicine ,Infectious Diseases ,medicine.anatomical_structure ,law ,Internal medicine ,medicine ,Vagina ,Sex organ ,030212 general & internal medicine ,Respiratory system ,business - Abstract
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is spread mainly through respiratory droplets or direct contact. However, the infection condition of the genital system is unknown. Our aim in this study was to determine if SARS-CoV-2 is present in the vaginal fluid of women with coronavirus disease 2019 (COVID-19). Methods Ten women with confirmed severe COVID-19 pneumonia admitted to the Tongji Zhongfa Hospital intensive care unit from 4 February 2020 through 24 February 2020 were included. Clinical records, laboratory results, and computed tomography examinations were retrospectively reviewed. The potential for genital infection was accessed by testing for the presence of SARS-CoV-2 in vaginal fluids obtained from vaginal swab samples. Reverse transcriptase polymerase chain reaction was used to confirm the SARS-CoV-2 infection in vaginal fluids. Results The clinical characteristics of the 10 women were similar to those reported in other severe COVID-19 patients. All 10 patients were tested for SARS-CoV-2 in vaginal fluid, and all samples tested negative for the virus. Conclusions Findings from this small group of cases suggest that SARS-CoV-2 virus does not exist in the vaginal fluids of severe COVID-19 patients.
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- 2020
32. Super-dominant pathobiontic bacteria in the nasopharyngeal microbiota as causative agents of secondary bacterial infection in influenza patients
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Zhifeng Qiu, Pengcheng Du, Junrong Liang, Taisheng Li, Taoran Geng, Hongyu Ren, Tian Qin, Yang Han, Tiekuan Du, Haijian Zhou, Jianguo Xu, Wei Jiang, and Xudong Nie
- Subjects
Adult ,Male ,0301 basic medicine ,Epidemiology ,Klebsiella pneumoniae ,030106 microbiology ,Immunology ,medicine.disease_cause ,Microbiology ,Article ,03 medical and health sciences ,Taxonomic composition ,lower-respiratory tract infection ,Nasopharynx ,Virology ,Lower respiratory tract infection ,Influenza, Human ,Drug Discovery ,medicine ,Humans ,super-dominant pathobiontic genus ,Aged ,biology ,Coinfection ,Pseudomonas aeruginosa ,Microbiota ,Bacterial Infections ,General Medicine ,Middle Aged ,16S ribosomal RNA ,biology.organism_classification ,medicine.disease ,16S rRNA V3–V4 ,Acinetobacter baumannii ,Influenza patients ,030104 developmental biology ,Infectious Diseases ,medicine.anatomical_structure ,Female ,Parasitology ,Bacteria ,nasopharyngeal microbiota ,Respiratory tract - Abstract
The source of secondary lower respiratory tract bacterial infections in influenza patients is not fully understood. A case–control study was conducted during the 2017–2018 influenza epidemic period in Beijing, China. Nasopharyngeal swabs were collected from 52 virologically confirmed influenza patients and 24 healthy medical staff. The nasopharyngeal microbiota taxonomic composition was analysed using high-throughput sequencing of the 16S rRNA gene V3–V4 regions. The super-dominant pathobiontic bacterial genus (SDPG) was defined as that accounting for >50% of sequences in a nasopharyngeal swab. We attempted to isolate bacteria of this genus from both nasopharyngeal swabs and lower-respiratory tract samples and analyse their genetic similarities. We observed a significantly lower taxonomy richness in influenza cases compared with healthy controls. A SDPG was detected in 61% of severe cases but in only 24% of mild cases and 29% of healthy controls. In 10 cases, the species isolated from lower-respiratory tract infection sites were identified as belonging to the nasopharyngeal microbiota SDPG. Genetically identical strains were isolated from both nasopharyngeal swabs and lower-respiratory tract infection sites, including 23 Acinetobacter baumannii strains from six severe cases, six Klebsiella pneumoniae strains from two severe cases, five Pseudomonas aeruginosa strains from one severe and one mild case, and four Corynebacterium striatum strains from two severe cases. The SDPG in the nasopharyngeal microbiota are the likely cause of subsequent infection in influenza patients.
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- 2020
33. Prolonged presence of viral nucleic acid in clinically recovered COVID-19 patients was not associated with effective infectiousness
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Wei Cao, Taisheng Li, Yuan Wei, Ke Hong, Li Chen, Lianguo Ruan, Xiaosheng Liu, Xing Zhou, Yan Zeng, Yang Han, Zhengyin Liu, and Ling Lin
- Subjects
Male ,0301 basic medicine ,Viral nucleic acid ,China ,medicine.medical_specialty ,Disease onset ,Coronavirus disease 2019 (COVID-19) ,Epidemiology ,Secondary infection ,Pneumonia, Viral ,030106 microbiology ,Immunology ,Polymerase Chain Reaction ,Microbiology ,prolonged viral RNA presence ,Betacoronavirus ,03 medical and health sciences ,Virology ,Internal medicine ,Drug Discovery ,medicine ,Humans ,infectiousness ,Viral rna ,Pandemics ,Aged ,SARS-CoV-2 ,business.industry ,COVID-19 ,General Medicine ,Middle Aged ,Disease control ,secondary transmission ,030104 developmental biology ,Infectious Diseases ,Nucleic acid ,RNA, Viral ,Original Article ,Female ,Parasitology ,Coronavirus Infections ,business ,Research Article ,Follow-Up Studies - Abstract
Prolonged presence of viral nucleic acid was reported in certain patients with coronavirus disease 2019 (COVID-19), with unclear clinical and epidemiological significance. We here described the clinical and epidemiological characteristics of 37 recovered COVID-19 patients with prolonged presence of viral RNA in Wuhan, China. For those who had been discharged and re-admitted, their close contacts outside the hospital were traced and evaluated. The median age of the 37 patients was 62 years (IQR 50, 68), and 24 (64.9%) were men. They had common or severe COVID-19. With prolonged positive RT-PCR, most patients were clinically stable, 29 (78.4%) denied any symptoms. A total of 431 PCR tests were carried out, with each patient at a median of 8 time points. The median time of PCR positivity to April 18 was 78 days (IQR 67.7, 84.5), and the longest 120 days. 22 of 37 patients had been discharged at a median of 44 days (IQR 22.3, 50) from disease onset, and 9 had lived with their families without personal protections for a total of 258 person-days and no secondary infection was identified through epidemiological investigation, nucleic acid and antibody screening. Infectiousness in COVID-19 patients with prolonged presence of viral nucleic acid should not solely be evaluated by RT–PCR. Those patients who have clinically recovered and whose disease course has exceeded four weeks were associated with very limited infectiousness. Reconsideration of disease control in such patients is needed.
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- 2020
34. Broadly neutralizing antibodies for HIV-1: efficacies, challenges and opportunities
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Ming Sun, Taisheng Li, Wei Cao, and Yubin Liu
- Subjects
0301 basic medicine ,Cart ,Epidemiology ,030106 microbiology ,Immunology ,Human immunodeficiency virus (HIV) ,HIV Infections ,challenges ,Review ,efficacies ,medicine.disease_cause ,Microbiology ,03 medical and health sciences ,opportunities ,broadly neutralizing antibodies (bNAbs) ,Virology ,Drug Resistance, Viral ,Drug Discovery ,Animals ,Humans ,Medicine ,Clinical Trials as Topic ,biology ,business.industry ,virus diseases ,General Medicine ,Antiretroviral therapy ,030104 developmental biology ,Infectious Diseases ,HIV-1 ,biology.protein ,Parasitology ,Antibody ,business ,Broadly Neutralizing Antibodies - Abstract
Combination antiretroviral therapy (cART) is effective but not curative, and no successful vaccine is currently available for human immunodeficiency virus-1 (HIV-1). Broadly neutralizing antibodies (bNAbs) provide a new approach to HIV-1 prevention and treatment, and these promising candidates advancing into clinical trials have shown certain efficacies in infected individuals. In addition, bNAbs have the potential to kill HIV-1-infected cells and to affect the course of HIV-1 infection by directly engaging host immunity. Nonetheless, challenges accompany the use of bNAbs, including transient suppression of viraemia, frequent emergence of resistant viruses in rebound viraemia, suboptimal efficacy in virus cell-to-cell transmission, and unclear effects on the cell-associated HIV-1 reservoir. In this review, we discuss opportunities and potential strategies to address current challenges to promote the future use of immunotherapy regimens.
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- 2020
35. China CDC’s HIV/AIDS Vaccine Efforts, from Basic Research to Clinical Studies
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Ying Liu, Jing Xu, Tong Zhang, Dan Li, Shuhui Wang, Yingqi Wu, Li Ren, Yiming Shao, Yanling Hao, Hao Wu, Taisheng Li, and Lihua Wu
- Subjects
medicine.medical_specialty ,Acquired immunodeficiency syndrome (AIDS) ,Basic research ,business.industry ,Family medicine ,MEDLINE ,Medicine ,General Agricultural and Biological Sciences ,China ,business ,medicine.disease ,Perspectives - Published
- 2020
36. A Review of Clinical Pharmacokinetic and Pharmacodynamic Profiles of Select Antiretrovirals: Focus on Differences among Chinese Patients
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Xiaoli Du, Taisheng Li, Qing Ma, Wenxiu Peng, Zhu Zhu, and Qiang Fu
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Nevirapine ,Efavirenz ,Anti-HIV Agents ,030106 microbiology ,Cmax ,HIV Infections ,030204 cardiovascular system & hematology ,03 medical and health sciences ,chemistry.chemical_compound ,Zidovudine ,0302 clinical medicine ,Asian People ,Internal medicine ,medicine ,Humans ,Pharmacology (medical) ,business.industry ,Racial Groups ,Lamivudine ,Lopinavir ,chemistry ,Area Under Curve ,Pharmacodynamics ,Ritonavir ,business ,medicine.drug - Abstract
Objective To identify the pharmacokinetic differences of antiretroviral drugs between HIV-infected Chinese patients and patients of other race/ethnicities. Study design Results from prospective, open-label pharmacokinetic studies among Chinese and historical data from other race/ethnicities. Patients Pharmacokinetics of six commonly used antiretroviral drugs, including zidovudine, lamivudine, tenofovir disoproxil fumarate, nevirapine, efavirenz and lopinavir/ritonavir, was evaluated in HIV-infected Chinese patients and compared with historical data from other race/ethnicities. Analysis Pharmacokinetic analyses were performed at the steady state among HIV-infected Chinese patients. Safety data were collected during the follow-up. The pharmacokinetic parameters including maximal concentrations (Cmax), area-under-curve (AUC) and clearance (Cl/F) from the Chinese patients were compared to the historic data from other race/ethnicities. Results Current evidence, though limited, suggested that these antiretroviral agents were generally safe and effective among HIV-infected Chinese patients. However, compared with other racial groups, Chinese patients exhibited higher Cmax , AUC and lower Cl/F for most of the agents, and the incidences of adverse reactions, for example, liver toxicity, rash, and bone health, were more frequent. Conclusions These pharmacokinetic differences suggest that lower dosages for commonly prescribed antiretroviral drugs in China might be appropriate to reduce drug-related adverse reactions, while maintain the antiviral efficacy.
- Published
- 2019
37. Combined multi-omics and network pharmacology approach reveals the role of Tripterygium Wilfordii Hook F in treating HIV immunological non-responders
- Author
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Xiaosheng Liu, Ling Lin, Tingxia Lv, Lianfeng Lu, Xiaodi Li, Yang Han, Zhifeng Qiu, Xiaoxia Li, Yanling Li, Xiaojing Song, Wei Cao, and Taisheng Li
- Subjects
Pharmacology ,Proteomics ,Complementary and alternative medicine ,Tripterygium ,Drug Discovery ,Leukocytes, Mononuclear ,Pharmaceutical Science ,Molecular Medicine ,Humans ,HIV Infections ,Network Pharmacology ,Drugs, Chinese Herbal - Abstract
The HIV-1 infected immunological non-responders (INRs) are characterized by poor immune reconstitution after long-term treatment. Tripterygium Wilfordii Hook F (TwHF) pill is a traditional Chinese patent drug with extensive immunosuppressive effects and has been clinically proven efficacy in treating INRs.The therapeutic mechanism of TwHF pills in the treatment of INRs was investigated by the combined multi-omics analysis on clinical samples and network pharmacology approach.Clinically, the peripheral blood mononuclear cells (PBMC) samples of TwHF-treated INRs from different time points were collected to conduct the transcriptomic and proteomic profiling. Key effector pathways of TwHF were enriched and analyzed by the ingenuity pathway analysis (IPA). Computationally, the TwHF-related compounds were obtained from traditional Chinese medicine databases, and literature search and structural prediction were performed to identify TwHF-related targets. Integrated with the INR-related targets, the 'TwHF-compounds-targets-INR' network was constructed to analyze core effector targets by centrality measurement. Experimentally, the effects of TwHF compounds on the T cells activation and expression of identified targets were evaluated with in vitro cell culture.33 INRs were included and treated with TwHF pills for 17 (IQR, 12-24) months. These patients experienced rapid growth in the CD4Our research is the first to systemic verify the mechanisms of TwHF in treating INRs. The IFN signaling pathway and the STAT1 would be the major effector targets of TwHF pills in treating INRs. The TPL would be the major bioactive compound to inhibit the IFN response and the phosphorylation of STAT1. Our observations suggest the basis for further application of TPL analogous in treating INRs.
- Published
- 2021
38. Safety and efficacy of long-acting intramuscular cabotegravir and rilpivirine in adults with HIV-1 infection: a systematic review and meta-analysis protocol
- Author
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Yuan-ni Wu, Hai Yu, Lianfeng Lu, Xiaodi Li, Xiaosheng Liu, Wei Cao, and Taisheng Li
- Subjects
Adult ,Anti-Retroviral Agents ,Meta-Analysis as Topic ,Pyridones ,Rilpivirine ,HIV-1 ,Humans ,HIV Infections ,General Medicine ,Systematic Reviews as Topic - Abstract
BackgroundCurrent antiretroviral regimens have, for the most part, achieved optimal antiretroviral efficacy and tolerability, transforming HIV infection from a deadly disease into a manageable chronic condition. However, adherence to daily oral drug intake remains an issue, as it is the most important determinant for sustained viral suppression and prevention of the emergence of drug-resistant viral strains. The long-acting injection antiretroviral cabotegravir and rilpivirine combination, a novel drug delivery approach, is about to revolutionise the therapy for people living with HIV. In this protocol, we aim to generate a clinically useful summary of the interventions based on their efficacy.Methods and analysisWe searched the literature for eligible studies published from inception up to 16 August 2022 through PubMed, EMBASE, Cochrane Library, Scopus and ClinicalTrials.gov. Two methodologically trained researchers will select the qualified studies for data extraction independently. Cochrane Risk of Bias tool will be used to assess the risk of bias in included studies. Statistical heterogeneity will be computed by Cochrane X2and I2tests. Sensitivity analysis will be conducted to evaluate the stability of the results. Publication biases will be evaluated by Begg’s and Egger’s tests. The quality of evidence will be assessed by the Grading of Recommendations Assessment, Development and Evaluation system. The RevMan V.5.3 and Stata V.14.0 software will be applied for statistical analyses.Ethics and disseminationEthical approval will not be required for this systematic review because the data used are not linked to the individual patient. The results of this review will be disseminated by being published in a peer-reviewed journal.PROSPERO registration numberCRD42022310414
- Published
- 2022
39. HIV-Related Immune Activation and Inflammation: Current Understanding and Strategies
- Author
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Taisheng Li, Wei Cao, and Tingxia Lv
- Subjects
CD4-Positive T-Lymphocytes ,Immunology ,Human immunodeficiency virus (HIV) ,Inflammation ,HIV Infections ,Disease ,Review Article ,CD8-Positive T-Lymphocytes ,medicine.disease_cause ,Lymphocyte Activation ,Virus Replication ,Antiretroviral Therapy, Highly Active ,medicine ,Immunology and Allergy ,Humans ,Lymphocyte Count ,Mechanism (biology) ,business.industry ,T-cell depletion ,General Medicine ,RC581-607 ,Antiretroviral therapy ,Clinical trial ,HIV-1 ,Immunologic diseases. Allergy ,medicine.symptom ,business ,Immune activation - Abstract
Although antiretroviral therapy effectively controls human immunodeficiency virus (HIV) replication, a residual chronic immune activation/inflammation persists throughout the disease. This aberrant immune activation and inflammation are considered an accelerator of non-AIDS-related events and one of the driving forces of CD4+ T cell depletion. Unfortunately, HIV-associated immune activation is driven by various factors, while the mechanism of excessive inflammation has not been formally clarified. To date, several clinical interventions or treatment candidates undergoing clinical trials have been proposed to combat this systemic immune activation/inflammation. However, these strategies revealed limited results, or their nonspecific anti-inflammatory properties are similar to previous interventions. Here, we reviewed recent learnings of immune activation and persisting inflammation associated with HIV infection, as well as the current directions to overcome it. Of note, a more profound understanding of the specific mechanisms for aberrant inflammation is still imperative for identifying an effective clinical intervention strategy.
- Published
- 2021
40. Association between tachyarrhythmia and mortality in a cohort of critically ill patients with coronavirus disease 2019 (COVID-19)
- Author
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Zhengyin Liu, Ran Tian, Yan Qin, Taisheng Li, Shuyang Zhang, Hao Qian, Xiu-Wu Bian, Xiaowei Yan, Peng Gao, Fan Guo, Dachun Zhao, Xue Lin, Wei Wu, Xiang Zhou, and Jinglan Wang
- Subjects
medicine.medical_specialty ,Myocarditis ,business.industry ,Proportional hazards model ,Hazard ratio ,Atrial fibrillation ,Retrospective cohort study ,General Medicine ,030204 cardiovascular system & hematology ,Lower risk ,medicine.disease ,Ventricular tachycardia ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Cohort ,Medicine ,Original Article ,030212 general & internal medicine ,business - Abstract
BACKGROUND: Cardiovascular involvement manifesting as arrhythmias has been confirmed in patients with coronavirus disease 2019 (COVID-19), so we aimed to explore the association between primary tachyarrhythmia and death in critically ill patients with COVID-19 in this retrospective study. METHODS: A total of 79 critically ill patients with COVID-19 were included. Demographic characteristics, clinical data (past history, vital signs, therapeutic management, and outcomes), and results of laboratory findings and cardiac investigations were collected. All statistical analyses were performed using SPSS 23.0 software (IBM, Armonk, NY, USA). RESULTS: The median age was 65±12 years, and 53 patients (67%) were male. A total of 57 (72%) patients died, and compared with survivors, these patients were older and had significantly higher Acute Physiology and Chronic Health Evaluation (APACHE) II score, Sequential Organ Failure Assessment (SOFA) score and fewer lymphocytes as well as higher heart rate (P
- Published
- 2021
41. Circulating Activated Immune Cells as a Potential Blood Biomarkers of Non-small Cell Lung Cancer Occurrence and Progression
- Author
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Hongsheng Liu, Changting Meng, Wei Liu, Rui Zhu, Yuping Ge, Taisheng Li, Zhao Sun, Na Zhou, Yang Gao, Yingyi Wang, Xiaoyuan Li, and Chunmei Bai
- Subjects
Adult ,Male ,Pulmonary and Respiratory Medicine ,Lung Neoplasms ,Lymphocyte ,T cell ,Clinicopathologic characteristics ,Basophil ,CD38 ,NSCLC ,Advance cancer stage ,Diseases of the respiratory system ,Immune system ,Carcinoma, Non-Small-Cell Lung ,Biomarkers, Tumor ,Tumor Microenvironment ,Humans ,Medicine ,Lymphocyte Count ,Lymphocytes ,Cancer occurrence ,Aged ,Retrospective Studies ,Aged, 80 and over ,RC705-779 ,business.industry ,Research ,Immune cells ,CD28 ,Middle Aged ,Eosinophil ,Flow Cytometry ,medicine.anatomical_structure ,Disease Progression ,Cancer research ,Female ,business ,CD8 - Abstract
Background Treatment for non-small cell lung cancer (NSCLC) has greatly improved in recent years. However, noninvasive early screening for carcinogenesis and progression unclear. The aim of this study was to explore the predictive value of peripheral blood immune cells in untreated NSCLC patients. Methods We retrospectively enrolled 305 untreated NSCLC patients and 132 healthy participants from February 2016 to August 2019 in Peking Union Medical College Hospital. Immune cell levels were determined by flow cytometry and routine blood tests. Results NSCLC patients had lower levels of T lymphocytes, NK cells, CD8+ T cells, naïve CD4+/CD4+, naïve CD4+ T cells and higher levels of CD4+ T cells, memory CD4+/CD4+ T cells, memory CD4+ T cells, CD4+CD28+/CD4+ T cells, CD4+CD28+ T cells, CD8+CD28+/CD8+ T cells, CD8+HLA-DR+/CD8+ T cells, CD8+HLA-DR+ T cells T cells, CD8+CD38+/CD8+ T cells, CD8+CD38+ T cells and CD4+/CD8+ T cells than those in controls. The percentages of specific lymphocyte subtypes were significantly different in cancer patients versus healthy individuals. For instance, cancer patients had lower levels of B cells, CD4+ T cells, naïve CD4+/CD4+ T cells, naïve CD4+ T cells, CD4+CD28+ T cells, CD8+CD28+ T cells and higher levels of NK cells, white blood cells (WBC), monocytes, neutrophils, eosinophils, basophils, monocytes to lymphocyte ratio (MLR), neutrophils to lymphocyte ratio (NLR), eosinophil to lymphocyte ratio (ELR), basophil to lymphocyte ratio (BLR), and blood platelet to lymphocyte ratio (PLR). Conclusions Abnormal T cell levels can be used as an independent predictive biomarker for noninvasive early screening in NSCLC occurrence and progression.
- Published
- 2021
42. Validation of a six-item dietary calcium screening tool among HIV patients in China
- Author
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Wenyan Shi, Wenni Xie, Evelyn Hsieh, Taisheng Li, Hui Lyu, Yun He, Zhangping Yu, Leslie Yingzhijie Tseng, Wei Pan, and Wei Chen
- Subjects
Adult ,Male ,medicine.medical_specialty ,China ,Osteoporosis ,Human immunodeficiency virus (HIV) ,Medicine (miscellaneous) ,HIV Infections ,medicine.disease_cause ,Diet Surveys ,Article ,Internal medicine ,medicine ,Humans ,Screening tool ,Dietary calcium ,Nutrition and Dietetics ,Receiver operating characteristic ,business.industry ,Public Health, Environmental and Occupational Health ,Reproducibility of Results ,medicine.disease ,Diet Records ,Calcium, Dietary ,Quartile ,Hiv patients ,business ,Relative validity - Abstract
Objective:Individuals with HIV are at increased risk for osteoporosis. A healthy diet with adequate Ca is recommended to promote bone health. However, lengthy nutritional assessments pose barriers to routine screenings in clinical practice. This study aimed to examine the validity and reproducibility of a six-item dietary Ca screening tool among Chinese individuals with HIV.Design:We conducted a two time-point study in an outpatient setting. Volunteers self-administered the six-item tool upon enrolment and again at 1-month follow-up. At baseline, participants also completed a validated FFQ and surveys regarding demographic and clinical risk factors.Setting:Beijing, China; Shenzhen, Guangdong, China.Participants:Upon enrolment, 127 individuals with HIV participated in the study, of whom 83 completed the follow-up screening.Results:Mean age of participants was 35·2 (sd 9·3) years, average BMI was 22·8 (sd 3·8) kg/m2 and 89 % were men. Among the participants, 54·7 % reported Ca intake less than 800 mg/d. The six-item tool demonstrated fair-to-moderate relative validity with a correlation of 0·39 and 75·7 % of subjects classified in same/adjacent quartiles as the reference, and moderate-to-good reproducibility with a correlation of 0·60 and 83·1 % of subjects classified in same/adjacent quartiles. Finally, receiver operating characteristic analyses yielded a sensitivity of 87·0 % and a specificity of 39·4 % with optimised cut-off level.Conclusions:The six-item tool presented adequate validity and reproducibility to identify individuals with low Ca intake among the target population, providing a convenient instrument for categorising Ca intake in clinical practice, prompting referrals for further assessment, and raising awareness of dietary Ca in bone disease prevention.
- Published
- 2021
43. An analysis of the feeding behavior of three stages of Toxoptera citricida by <scp>DC</scp> electrical penetration graph waveforms
- Author
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Xuefeng Wang, Changyong Zhou, Runa Zhao, Yingqin He, Zhuoyue Lu, Wenlong Chen, and Taisheng Li
- Subjects
Citrus unshiu ,Horticulture ,Feeding behavior ,Rutaceae ,biology ,Insect Science ,Electrical penetration graph ,Aphididae ,biology.organism_classification ,Nymph ,Toxoptera citricida ,Hemiptera ,Ecology, Evolution, Behavior and Systematics - Published
- 2019
44. Cerebral Vasoreactivity Evaluated by the Breath-Holding Challenge Correlates With Performance on a Cognitive Screening Test in Persons Living With Treated HIV Infection in China
- Author
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Natasha Mehta, Taisheng Li, Huanling Wang, Wei Lü, Felicia C. Chow, Jing Xie, Weihai Xu, Yang Han, Yanling Li, and Yinghuan Hu
- Subjects
Adult ,Male ,China ,medicine.medical_specialty ,AIDS Dementia Complex ,Urban Population ,Anti-HIV Agents ,Human immunodeficiency virus (HIV) ,MEDLINE ,HIV Infections ,Pilot Projects ,medicine.disease_cause ,Article ,Breath Holding ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Mass Screening ,Medicine ,Pharmacology (medical) ,030212 general & internal medicine ,Diagnostic Tests, Routine ,business.industry ,Extramural ,Diagnostic test ,Middle Aged ,Test (assessment) ,Infectious Diseases ,Cognitive screening ,Emergency medicine ,Female ,Cerebral Arterial Diseases ,business ,030217 neurology & neurosurgery - Published
- 2018
45. Naïve CD4
- Author
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Ling, Xu, Yubin, Liu, Xiaojing, Song, Yanling, Li, Yang, Han, Ting, Zhu, Wei, Cao, and Taisheng, Li
- Subjects
Adult ,CD4-Positive T-Lymphocytes ,Male ,Immune activation ,LTNPs ,naïve CD4+ T cell count diminishing ,Research ,High HIV RNA levels ,HIV Infections ,CD8-Positive T-Lymphocytes ,Viral Load ,Lymphocyte Activation ,Cohort Studies ,Memory T Cells ,Blood products ,T-Lymphocyte Subsets ,Disease Progression ,HIV Non-Progressors ,HIV-1 ,Humans ,Lymphocyte Count ,Follow-Up Studies - Abstract
Background Some long-term non-progressors (LTNPs) have decreasing CD4+ T cell counts and progress to AIDS. Exploring which subsets of CD4+ T cell decreasing and the determinants associated with the decay in these patients will improve disease progression surveillance and provide further understanding of HIV pathogenesis. Methods Twenty-five LTNPs infected with HIV by blood products were classified as decreased (DG) if their CD4+ cell count dropped to
- Published
- 2021
46. Clinical characteristics and survival analysis in critical and non-critical patients with COVID-19 in Wuhan, China: a single-center retrospective case control study
- Author
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Jihua Shi, Jinglan Wang, Chunyao Wang, Peng Gao, Taisheng Li, Zhiyu Zhang, Yan Qin, Fan Guo, Shuyang Zhang, Hao Qian, Ran Tian, Zhengyin Liu, Wenbin Li, Xiang Zhou, Wei Wu, Qingfeng Luo, Haopeng Xu, Xiaowei Yan, and Haiyu Pang
- Subjects
Male ,medicine.medical_specialty ,Organ Dysfunction Scores ,Pneumonia, Viral ,lcsh:Medicine ,Kaplan-Meier Estimate ,Single Center ,Severity of Illness Index ,Article ,Fibrin Fibrinogen Degradation Products ,Betacoronavirus ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Severity of illness ,Epidemiology ,Odds Ratio ,medicine ,Humans ,Hospital Mortality ,Lymphocyte Count ,030212 general & internal medicine ,lcsh:Science ,Pandemics ,Survival analysis ,Aged ,Retrospective Studies ,Respiratory tract diseases ,Multidisciplinary ,Interleukin-6 ,SARS-CoV-2 ,business.industry ,lcsh:R ,Case-control study ,COVID-19 ,Retrospective cohort study ,Odds ratio ,Middle Aged ,Logistic Models ,Case-Control Studies ,030220 oncology & carcinogenesis ,Infectious diseases ,lcsh:Q ,Female ,SOFA score ,Coronavirus Infections ,business - Abstract
Since the outbreak of COVID-19 in China at the end of 2019, the world has experienced a large-scale epidemic caused by the SARS-CoV-2. Epidemiological and clinical course of COVID-19 patients have been reported, but there have been few analyses about the characteristics, predictive risk factors and outcomes of critical patients. In this single-center retrospective case-control study, 90 adult inpatients hospitalized at Tongji Hospital (Wuhan, China) were included. Demographic, clinical, laboratory test and treatment data were obtained and compared between critical and non-critical patients. We found that compared with non-critical patients, the critical patients had higher SOFA score and qSOFA scores. Critical patients had lower lymphocyte and platelet count, elevated D-dimer, decreased fibrinogen, and elevated high-sensitivity C-reactive protein (hsCRP) and interleukin-6(IL-6). More critical patients received treatment including antibiotics, anticoagulation, corticosteroid and oxygen therapy than non-critical ones. Multivariable regression showed higher qSOFA score and elevation of IL-6 were related to critical patients. Antibiotic usage and anticoagulation were associated with decreased in-hospital mortality. And critical grouping contributed greatly to in-hospital death. Critical COVID-19 patients have a more severe clinical cours. qSOFA score and elevation of IL-6 are risk factors for critical condition. Non-critical grouping, positive antibiotic application and anticoagulation may be beneficial for patient survival.
- Published
- 2020
47. Long-term trabecular bone score and bone mineral density changes in Chinese antiretroviral-treated HIV-infected individuals
- Author
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Yanling Li, Zaizhu Zhang, Wei Pan, Wei Cao, Junping Tian, Xiaojing Song, Wenmin Guan, Ying Xu, Qiang Lin, Wei Yu, Evelyn Hsieh, and Taisheng Li
- Subjects
musculoskeletal diseases ,0301 basic medicine ,Adult ,medicine.medical_specialty ,China ,030209 endocrinology & metabolism ,HIV Infections ,Article ,03 medical and health sciences ,0302 clinical medicine ,Trabecular bone score ,Absorptiometry, Photon ,Bone Density ,Internal medicine ,Chart review ,Hiv infected ,medicine ,Humans ,Orthopedics and Sports Medicine ,Femoral neck ,Retrospective Studies ,Bone mineral ,Lumbar Vertebrae ,business.industry ,musculoskeletal system ,Regimen ,medicine.anatomical_structure ,Decreased bone mineral density ,Orthopedic surgery ,Cancellous Bone ,030101 anatomy & morphology ,business - Abstract
This is the first 5-year analysis among persons with HIV (PWH) that reports both trabecular bone score (TBS), which is a novel index that estimates bone microarchitecture, and bone mineral density (BMD) over time to evaluate the long-term impact of antiretroviral therapy (ART) on bone health. PURPOSE: HIV infection and antiretroviral therapy (ART) have been associated with decreased bone mineral density (BMD). This study aims to evaluate long-term changes in trabecular bone score (TBS), a novel index that estimates bone microarchitecture, and BMD among Chinese persons with HIV (PWH) treated with ART. METHODS: We conducted a retrospective chart review of adult PWH at a large tertiary care hospital in China. Patients who had a DXA scan prior to ART and at least one follow-up DXA after ART initiation were included. Subgroup analyses examined the TBS and BMD changes in patients who switch from a non-TDF-containing regimen to one containing TDF, as compared to those who did not switch. RESULTS: Four hundred fifty-nine PWH were included. Among 68 patients ≥ 50 years, 13 patients (19.1%) had a normal BMD but partially degraded or degraded TBS. The mean percent decrease in lumbar spine (LS) BMD nadired at 48 weeks after ART initiation and then gradually improved. Percent decrease in femoral neck (FN) and total hip (TH) BMD nadired at 96 weeks and remained stably low thereafter. After switch to a TDF-containing regimen, only percent change in TH BMD was significant (−3.2%, p = 0.006). In the regression analyses, switch to a TDF-containing regimen was not associated with long-term change in TBS or BMD. CONCLUSION: This is the first study among PWH to evaluate the long-term impact of ART on TBS and BMD. At baseline, approximately 20% of patients had a normal BMD but impaired bone microstructure based upon TBS. For patients with 5 years of exposure to ART, there is a stabilization of TBS and BMD after initial nadir in the first 144 weeks. However, FN BMD, TH BMD, and TBS remained low at 5 years relative to baseline.
- Published
- 2020
48. Whole blood as an alternative to peripheral blood mononuclear cell for detection of total HIV-1 DNA
- Author
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Zhifeng Qiu, Wei Cao, Lei-Yan Wei, Yang Han, Taisheng Li, Yongsong Yue, Ling Lin, Xiaojing Song, and Nidan Wang
- Subjects
0301 basic medicine ,Adult ,Male ,Anti-HIV Agents ,CD4-CD8 Ratio ,HIV Infections ,HIV-1 DNA ,Real-Time Polymerase Chain Reaction ,Peripheral blood mononuclear cell ,lcsh:Infectious and parasitic diseases ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Humans ,lcsh:RC109-216 ,030212 general & internal medicine ,Hiv 1 dna ,Whole blood ,Retrospective Studies ,Reservoir ,business.industry ,Viral Load ,AIDS ,030104 developmental biology ,Infectious Diseases ,Real-time polymerase chain reaction ,Parasitology ,DNA, Viral ,HIV-1 ,Leukocytes, Mononuclear ,Female ,business ,Viral load ,CD8 ,Student's t-test ,Follow-Up Studies ,Research Article - Abstract
Background A more time saving, convenient, reproducible, and scalable method is needed to assess total HIV-1 DNA levels. Methods Frozen whole blood and peripheral blood mononuclear cell (PBMC) samples both 200 μl at the same point were used to detect total HIV-1 DNA. Automatic extraction of total HIV-1 DNA was used to ensure the consistency of sample extraction efficiency. The detection reagent was HIV-1 DNA quantitative detection kit and real-time quantitative PCR was utilized. Results Of the 44 included patients, 42 were male and 2 were female, with a median age of 33 years. Thirty-three cases were collected after receiving antiviral treatment, with a median duration of treatment of 3 months, and the other 11 cases were collected before antiviral treatment. The median viral load was 1.83 log10 copies/mL, the median CD4 and CD8 count were 94 and 680 cells/μL, and the median CD4/CD8 ratio was 0.18. The results of the two samples were 3.02 ± 0.39 log10 copies/106 PBMCs in PBMC samples and 3.05 ± 0.40 log10 copies/106 PBMCs in whole blood samples. The detection results of the two methods were highly correlated and consistent by using paired t test (P = 0.370), pearson correlation (r = 0.887, P P Conclusions The results of the whole blood sample test for total HIV-1 DNA are consistent with those of PBMC samples. In a clinical setting it is recommended to use whole blood samples directly for the evaluation of the HIV reservoir.
- Published
- 2020
49. Immune checkpoint inhibitor therapy for cancer patients infected with HIV: A systematic review
- Author
-
Yan Xu, Ling Luo, and Taisheng Li
- Subjects
Oncology ,medicine.medical_specialty ,Lung Neoplasms ,Population ,HIV Infections ,Pembrolizumab ,Malignancy ,03 medical and health sciences ,0302 clinical medicine ,Carcinoma, Non-Small-Cell Lung ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,education ,Adverse effect ,Immune Checkpoint Inhibitors ,education.field_of_study ,business.industry ,Cancer ,General Medicine ,medicine.disease ,Clinical trial ,Nivolumab ,Tolerability ,030220 oncology & carcinogenesis ,business ,hormones, hormone substitutes, and hormone antagonists - Abstract
BACKGROUND Immune checkpoint inhibitor (ICI) therapy represents a breakthrough in cancer treatment. However, clinical trials of ICI have usually excluded people living with HIV (PLWH). The efficacy and safety data of ICI in PLWH is scarce. METHODS Literature on the efficacy and safety of ICI in PLWH with advanced cancers and on the role in decreasing HIV reservoir were searched via PubMed. RESULTS One hundred and thirty patients were identified. The most commonly used ICIs are nivolumab (48.5%) and pembrolizumab (36.9%). The most common malignancy in this population analyzed in this review is non-small cell lung cancer (NSCLC, 43.1%). The control rates of ICIs observed for PLWH with advanced NSCLC was consistent with that reported for general population. In this analysis, ICI therapy was generally well tolerated, with grade 3 or higher adverse events in 15 of 130 patients (11.5%) and has not deleterious effects on HIV virologic control. ICI has also shown a role in decreasing HIV reservoir. CONCLUSIONS The review shows that ICI appears to be feasible in this specific population. Efficacy and tolerability seemed to be comparable with that of general population with advanced cancers.
- Published
- 2020
50. Incidence of hypertension among persons living with HIV in China: a multicenter cohort study
- Author
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Wei-Ti Chen, Wei Lv, Xiaojing Song, Jing Xie, Yanling Li, Yang Han, Fuping Guo, Evelyn Hsieh, Hongwei Fan, and Taisheng Li
- Subjects
Adult ,Male ,China ,medicine.medical_specialty ,Clinical Trials and Supportive Activities ,HIV Infections ,Comorbidity ,030204 cardiovascular system & hematology ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Clinical Research ,Internal medicine ,Epidemiology ,Humans ,Medicine ,Longitudinal Studies ,Prospective Studies ,030212 general & internal medicine ,Proportional Hazards Models ,business.industry ,Proportional hazards model ,lcsh:Public aspects of medicine ,Incidence ,Prevention ,Incidence (epidemiology) ,Hazard ratio ,Public Health, Environmental and Occupational Health ,HIV ,lcsh:RA1-1270 ,Middle Aged ,Confidence interval ,Infectious Diseases ,Good Health and Well Being ,Hypertension ,Public Health and Health Services ,HIV/AIDS ,Female ,Public Health ,Infection ,business ,Viral load ,Body mass index ,Research Article ,Cohort study - Abstract
BackgroundLife expectancy among persons living with HIV (PLWH) has improved with increasing access to antiretroviral therapy (ART), however incidence of chronic comorbidities has simultaneously increased. No data are available regarding the incidence of hypertension among Chinese PLWH.MethodsWe analyzed data collected from patients enrolled in two prospective longitudinal multicenter studies of PLWH initiating ART in China. Incidence rate of hypertension per 100 person-years (PYs) among PLWH was calculated, and Cox proportional hazards models was used to evaluate the association between incident hypertension and traditional and HIV-associated risk factors.ResultsOf 1078 patients included in this analysis, 984 ART-naïve patients were hypertension-free at baseline, and contributed 2337.7 PYs of follow up, with a median follow-up period of 1.8 years (range: 1.2–3.2) after initiation of ART. Incidence of hypertension was 7.6 [95% confidence interval (CI): 6.5–8.7] per 100 PYs. In the Cox regression analysis, incidence of hypertension was positively associated with body mass index [adjusted hazard ratio (aHR) 1.07 (1.01,1.13),p = 0.02] and recent viral load (aHR 1.28, 95% CI:1.08–1.51,p p p ConclusionsThe incidence of hypertension was relatively high among Chinese PLWH initiating ART. Recent low CD4+/CD8+ ratio and detectable HIV viremia were associated with incident hypertension, whereas receipt of ART was associated with reduced risk. Hypertension may be mitigated, in part, by excellent HIV care, including viral suppression with ART.Trial registrationClinicalTrials.gov Identifier:NCT00872417registered on 31 March, 2009, andNCT01844297registered on 1 May, 2013.
- Published
- 2020
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