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Additional file 3: Table S1. 15q13.3 microdeletion syndrome and corresponding mutant mice. –: not assessed, n.s.: no significant difference, M: male, F: female, Ref: references.

Additional file 2: Figure S2. Normal biomonoamine levels in the brains (except the cerebellum) of Trpm1−/− mice. Quantification of monoamine neurotransmitters in brain regions except the cerebellum at 4 months old. n = 24 for both genotypes. No significant changes; Student’s t test or Welch’s t test.

Additional file 1: Figure S1. Behavioral and physiological characteristics of Trpm1−/− mice. (A–E) General health and neurological screen; (A) body weight, (B) body temperature, (C) grip strength, (D) wire hang test, (E) latency to fall in the rotarod test. n = 4 for both genotypes (A–D), n = 23 for both genotypes (E). (F–K) Gait analysis of front and hind paws; (F) stride duration, (G) stance duration, (H) stride length, (I) stance width, (J) step angle, (K) paw angle. n = 24 Trpm1−/−, n = 23 WT. (L, M) Porsolt forced swimming test; (L) distance traveled, and (M) proportion of time spent immobile in each 1-min period. n = 24 for both genotypes. (N) Percentage of time spent immobile in each 1-min period in the tail suspension test. n = 24 for both genotypes. (O) Serum corticosterone was measured at 4 months of age. n = 4 for both genotypes. (P) Social activity averaging 3 days in home cage test. n = 22 Trpm1−/−, n = 21 WT. *P

Figure S2. Withdrawal threshold to mechanical stimulation in von Frey test. Basal mechanical sensitivity was evaluated by von Frey test. Withdrawal threshold of wild-type (WT, white column) and Caskin1-KO (black column) mice were assessed in 8–15 week-old mice. Data are expressed as described in “Material and Methods” as the mean ± SEM (n = 17–18). P-value indicates genotype effect in Mann-Whitney U-test. (PDF 55 kb)