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The downward shortwave radiation (DSR) is a key input parameter for land surface models and climate models. Based on the daily averaged Global Land Surface Satellite downward shortwave radiation (GLASS-DSR) dataset over the Yunnan-Kweichow Plateau (YKP) from 1984 to 2018, this paper analyzes variation trend and breakpoints of DSR. The results show that: annual averaged DSR decreases at a decreasing rate of −1.84 W·m−2·decade−1 over the YKP from 1984 to 2018; the overall distribution of interannual averaged DSR shows higher in the mid-west, and gradually decreasing from west to northeast over the YKP; the estimated averaged DSR is larger in spring than in summer due to the influence of the monsoon; monthly averaged DSR reaches its maximum in May and its minimum in December; breakpoints are found in the seasonal and trend components of daily averaged DSR. Eleven driving factors are examined for their effects on DSR variation, including annual average temperature, precipitation, 10 m wind speed, aerosol optical thickness (AOT), total cloud cover, elevation, slope, aspect, longitude, latitude, and climate zones. According to the findings, AOT predominates in the spatio-temporal distribution of DSR over the YKP. This study will contribute to studies related to climate change and highland radiation.