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We Media is a way for the general public to disseminate their facts and news to the outside world through Internet or other means. It is a way for the general public to provide and share their information after being connected to the global knowledge system. This study is to explore the communicators, the content and effect of the dissemination of We Media. The dissemination of traditional culture not only provides the audience with rich cultural knowledge but also has great importance in shaping their values. From the cases of Li Ziqi and Dianxi Xiaoge, we can find that this strategy requires the vloggers to master a combination of skills, not only in filming and editing but also in cultural understanding and learning ability.

The nucleus in many cell types is a stiff organelle, but fat-filled lipid droplets (FDs) in cytoplasm are seen to indent and displace the nucleus. FDs are phase-separated liquids with a poorly understood interfacial tension γ that determines how FDs interact with other organelles. Here, micron-sized FDs remain spherical as they indent peri-nuclear actomyosin and the nucleus, while causing local dilution of Lamin-B1 independent of Lamin-A,C and sometimes triggering nuclear rupture. Focal accumulation of the cytosolic DNA sensor cGAS at the rupture site is accompanied by sustained mislocalization of DNA repair factors to cytoplasm, increased DNA damage, and delayed cell cycle. Macrophages show FDs and engulfed rigid beads cause similar indentation dilution. Spherical shapes of small FDs indicate a high γ, which we measure for FDs mechanically isolated from fresh adipose tissue as ∼40 mN/m. This value is far higher than that of protein condensates, but typical of oils in water and sufficiently rigid to perturb cell structures including nuclei.

Рассматривается ветвящееся случайное блуждание $(V_u)_{u\in \mathcal T^{\mathrm{IGW}}}$ в пространстве $\mathbb Z^d$ с генеалогическим деревом $\mathcal T^{\mathrm{IGW}}$, образованным последовательностью независимых одинаково распределенных критических деревьев Гальтона-Ватсона. Пусть $R_n$ - множество точек в пространстве $\mathbb Z^d$, которые посещает ветвящееся случайное блуждание $(V_u)$, когда индекс $u$ пробегает первые $n$ поддеревьев дерева $\mathcal T^{\mathrm{IGW}}$. Основной результат работы состоит в том, что для $d\in \{3,4,5\}$ при $n\to \infty $ емкость множества $R_n$ имеет почти наверное порядок $n^{(d-2)/{2}+o(1)}$.

Alpha-hemolysin (Hla) is one of the important exotoxins of

Research on shear thickening colloidal suspensions demonstrates that measurements of the microstructure can elucidate the source of the rheological material properties in the shear thickened state as well as critically test simulations and theory based on a variety of mechanisms such as enhanced lubrication hydrodynamics, elastohydrodynamics, and contact friction. Prior work on continuous shear thickening dispersions with a well-defined shear thickened state identified the formation of hydroclusters as characteristic of this state, determined the anisotropy in the nearest neighbor distribution, and used this information to test prevailing theories and simulations. However, important questions remain about the mesoscale (

Alpha-hemolysin (Hla) is one of the important exotoxins of Staphylococcus aureus (S. aureus) and can be used as a target to reduce the virulence of S. aureus. This study explored the inhibitory effect of Lysine (Lys) on Hla and its application in food safety. Lys significantly inhibited the expression of Hla at sub-inhibitory concentrations and directly interacted with Hla to interfere with its oligomerization and thus significantly inhibited its hemolytic activity. Notably, Lys attenuated S. aureus damage to mouse small intestine and Caco-2 cells and delayed mouse mortality. In the food model, Lys inhibited the expression of Hla of S. aureus and had no significant effect on the sensory score. Moreover, Lys had no obvious damage effect on the main organs of mice, which indicated that Lys has good biocompatibility and has the potential to be used in the food industry as an anti-S. aureus preparation.

This paper introduces a heuristic tentacle algorithm for local path planning of unmanned skid-steering vehicle. Mobility, safety and economy are three mainly focused aspects in the navigation of unmanned ground vehicles. Critical skidding and slipping often occur during the turning motion, which effect the vehicle's motion apparently. So vehicle kinematics are discussed and applied to construct the cluster of tentacles. Several path assessment criteria named obstacle avoidance, terrain roughness and distance to the global path are discussed. Based on the multi-density clustering processed in the global path planning, heuristic method is introduced to guide to search in sparse region. The simulation analysis shows the generated local path can avoid the obstacles along the global path. Simultaneously. the global path can be smoothed through kinematic aware tentacle algorithm.

ISWI chromatin remodelers mobilize nucleosomes to control DNA accessibility. Complexes isolated to date pair one of six regulatory subunits with one of two highly similar ATPases. However, we find that each endogenously expressed ATPase co-purifies with every regulatory subunit, substantially increasing the diversity of ISWI complexes, and we additionally identify BAZ2B as a novel, seventh regulatory subunit. Through reconstitution of catalytically active human ISWI complexes, we demonstrate that the new interactions described here are stable and direct. Finally, we profile the nucleosome remodeling functions of the now expanded family of ISWI chromatin remodelers. By revealing the combinatorial nature of ISWI complexes, we provide a basis for better understanding ISWI function in normal settings and disease.

By introducing a new measure for the infinite Galton-Watson process and providing estimates for (discrete) Green's functions on trees, we establish the asymptotic behavior of the capacity of critical branching random walks: in high dimensions $d\ge 7$, the capacity grows linearly; and in the critical dimension $d=6$, it grows asymptotically proportional to $\frac{n}{\log n}$., Comment: 33 pages, 7 figures

Path planning is one of the key abilities in artificial intelligence. Many different approaches exist, focusing on finding the shortest path. Obstacle density, which is often ignored by conventional approaches, has a great influence on path quality especially in off-road environment. Because obstacles along a path compromise driving safety and frequent obstacle avoidance increases vehicle control difficulty while decreases traveling speed. In this paper, a simple and efficient approach of hierarchical path planning algorithm based on multi-density clustering is proposed, aiming at finding a clear and short path to achieve an integrated goal of obstacle avoidance and path length shortness. A simulation evaluation shows that our proposed approach can find short path bypassing regions with dense obstacles efficiently.

Two colloidal suspensions of paucidisperse, spherical silica particles with different surface chemistries leading to extreme limits of surface contact friction are studied to identify experimental differences in shear rheology and microstructure and quantitatively test theory and simulation models. The nonequilibrium microstructure in the plane of shear is measured by flow-small angle neutron scattering for steady shear states spanning the shear thinning and shear thickening regimes. The shear rheology and microstructure are compared against predictions from theory for Brownian hard sphere suspensions and state-of-the-art simulation methods that incorporate either contact friction or enhanced lubrication hydrodynamics. The first normal stress differences are confirmed to distinguish between these micromechanical mechanisms for stress enhancement in the shear thickened regime. The nonequilibrium microstructure in the plane of shear shows more anisotropy for the suspension with higher interparticle friction. A significant fourfold symmetry is confirmed and found to be amplified with increasing surface contact friction in the shear thickened state. The differences in shear-induced microstructures between suspensions with varying contact friction demonstrate that the nonequilibrium microstructure can distinguish between nanotribological interactions in the shear thickened state. Statistical comparison of experiments with simulations indicates that better resolution of microstructures in simulation models is required to be validated by the experimental data presented. Implications for the development of theories for colloidal suspension rheology are discussed.

Members of the ISWI family of chromatin remodelers mobilize nucleosomes to control DNA accessibility and, in some cases, are required for recovery from DNA damage. However, it remains poorly understood how the non-catalytic ISWI subunits BAZ1A and BAZ1B might contact chromatin to direct the ATPase SMARCA5. Here, we find that the plant homeodomain of BAZ1A, but not that of BAZ1B, has the unusual function of binding DNA. Furthermore, the BAZ1A bromodomain has a non-canonical gatekeeper residue and binds relatively weakly to acetylated histone peptides. Using CRISPR-Cas9-mediated genome editing we find that BAZ1A and BAZ1B each recruit SMARCA5 to sites of damaged chromatin and promote survival. Genetic engineering of structure-designed bromodomain and plant homeodomain mutants reveals that reader modules of BAZ1A and BAZ1B, even when non-standard, are critical for DNA damage recovery in part by regulating ISWI factors loading at DNA lesions and supporting transcriptional programs required for survival., ISWI chromatin remodelers regulate DNA accessibility and have been implicated in DNA damage repair. Here, the authors uncover functions, in response to DNA damage, for the bromodomain of the ISWI subunit BAZ1B and for the non-canonical PHD and bromodomain modules of the paralog BAZ1A.

Defense engineering in Henan area is mainly the local fortress, but some areas have not form a fortress, but emphasize courtyard defense. This paper will analyze the composition and characteristics of courtyard defense engineering of Henan traditional dwellings through the on-the-spot investigation, hoping to show the defense engineering of Henan traditional dwellings which is different from fortress system.