1. Baseline circulating stem-like cells predict survival in patients with metastatic breast Cancer
- Author
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Yung-Chan Lin, Tzu-Tsen Yen, Feng-Che Kuan, Tyler Min-Hsien Wu, Jen-Shi Chen, Chun-Hui Lee, Ting-Shiuan Yeh, Wen-Chi Chou, Pei-Hung Chang, Ching-Chih Hu, Wen-Kuan Huang, Jason Chia-Hsun Hsieh, Chia-Lin Lee, Hung-Ming Wang, Tsung-Min Hung, and Sheng-Chieh Chan
- Subjects
Adult ,0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Breast Neoplasms ,Cell Count ,lcsh:RC254-282 ,03 medical and health sciences ,Breast cancer ,0302 clinical medicine ,Circulating tumor cell ,Cancer stem cell ,Surgical oncology ,Internal medicine ,Genetics ,medicine ,Humans ,Chemotherapy ,AC133 Antigen ,Prospective Studies ,CD133 ,Prospective cohort study ,Aged ,Aged, 80 and over ,Cancer stem cells ,business.industry ,Carcinoma, Ductal, Breast ,Liquid Biopsy ,Circulating tumor cells ,Cancer ,Middle Aged ,Neoplastic Cells, Circulating ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Survival Analysis ,Metastatic breast cancer ,Treatment Outcome ,030104 developmental biology ,030220 oncology & carcinogenesis ,Neoplastic Stem Cells ,Female ,business ,Research Article - Abstract
Background Circulating tumor cells (CTCs) are associated with breast cancer prognosis. Research is limited regarding the role of circulating cancer stem-like cells (cCSCs) considering the treatment response and survival among patients with metastatic breast cancer. Accordingly, we performed this prospective study to clarify the prognostic significance of baseline cCSCs for metastatic breast cancer in terms of first-line chemotherapy. Methods Between April 2014 and January 2016, we prospectively enrolled 48 patients with stage IV breast invasive ductal carcinoma who underwent first-line chemotherapy. We identified and analyzed CTCs and cCSCs by using a protocol based on negative selection and flow cytometry before chemotherapy. CTCs were identified as EpCAM+Hoechst+CD45– cells and cCSCs as CD133+EpCAM+Hoechst+CD45– cells. cCSCs were expressed as a percentage of CTCs. The associations between CTCs, cCSCs, and the clinicopathological variables that were predictive of the treatment response and survival outcome were analyzed using univariate and multivariate analyses. Results We identified CTCs in all the enrolled patients, with a median number of 33.9/mL CTCs. CSCs were isolated in 97.9% of the patients; the median percentage of cCSCs was 14.7%. A high baseline level of cCSCs was correlated with an inferior tumor response rate (54.2% vs. 95.8%, p Conclusions Baseline cCSCs predict the treatment response as well as survival in patients with metastatic breast cancer undergoing first-line chemotherapy. Therefore, the measurement of cCSCs may assist in identifying early cancer treatment response and prognosis.
- Published
- 2019