1. Self-assembled raccoon dog parvovirus VP2 protein confers immunity against RDPV disease in raccoon dogs: in vitro and in vivo studies
- Author
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Lei Wang, Mingjie Wu, Linya Xia, Yuhe Yin, Guoliang Luo, Congmei Wu, and Ning Zhang
- Subjects
0301 basic medicine ,viruses ,030106 microbiology ,Infectious and parasitic diseases ,RC109-216 ,Biology ,Antibodies, Viral ,Parvoviridae Infections ,Parvovirus ,03 medical and health sciences ,Virus-like particle ,Immunity ,In vivo ,Virology ,Escherichia coli ,Animals ,Vaccines, Virus-Like Particle ,Hemagglutination assay ,Research ,Antibody titer ,virus diseases ,Raccoon Dogs ,Virus like particle ,biology.organism_classification ,Immunogenicity ,030104 developmental biology ,Infectious Diseases ,Vaccines, Inactivated ,Trigger factor 16 ,Inactivated vaccine ,Capsid Proteins ,Raccoons ,Raccoon dog parvovirus - Abstract
Background Raccoon dog parvovirus (RDPV) causes acute infectious diseases in raccoon dogs and may cause death in severe cases. The current treatment strategy relies on the extensive usage of classical inactivated vaccine which is marred by large doses, short immunization cycles and safety concerns. Methods The present study aimed at optimization of RDPV VP2 gene, subcloning the gene into plasmid pET30a, and its subsequent transfer to Escherichia coli with trigger factor 16 for co-expression. The protein thus expressed was purified with ammonium sulfate precipitation, hydrophobic chromatography, and endotoxin extraction procedures. VLPs were examined by transmission electron microscopy, dynamic light scattering, and the efficacy of VLPs vaccine was tested in vivo. Results Results indicated that RDPV VP2 protein could be expressed soluble. Transmission electron microscopy and dynamic light scattering results indicated that RDPV VP2 self-assembled into VLPs. Hemagglutination inhibition antibody titers elicited by Al(OH)3 adjuvanted RDPV VLPs were comparable with RDPV inactivated vaccines, and the viral loads in the blood of the struck raccoon dogs were greatly reduced. Hematoxylin and eosin and Immunohistochemical results indicated that RDPV VLPs vaccine could protect raccoon dogs against RDPV infections. Conclusions These results suggest that RDPV VLPs can become a potential vaccine candidate for RDPV therapy.
- Published
- 2021