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Text S1. Capture methods. Figure S1. Epsilon-trap as used for sampling tsetse species of the Morsitans group found in southern Africa. Figure S2. Vehicle-mounted electric target (VET). Figure S3. Artificial refuge - photograph and plan. Text S2. Tsetse life-cycle. Text S3. Ovarian dissection: estimation of fly age. Figure S4. Diagrammatic representation of the relative sizes of oocytes of female tsetse (Glossina spp) during successive ovulation cycles. Text S4 Climate profiles at Rekomitjie Research Station. Figure S5. Average monthly maximum and minimum temperatures and rainfall for the period 1 January 1989 to 31 December 1993 at Rekomitjie Research Station. Table S1. Multivariable analysis of the effects of ovarian age, month, year and method of capture, and interactions between age and the three other variables, on the mean wing length of female tsetse captured in the field at Rekomitjie Research Station, 1989â 1993. (DOCX 961 kb)